IT1303767B1 - Metodo di quantificazione di acidi nucleici. - Google Patents
Metodo di quantificazione di acidi nucleici.Info
- Publication number
- IT1303767B1 IT1303767B1 IT1998MI002491A ITMI982491A IT1303767B1 IT 1303767 B1 IT1303767 B1 IT 1303767B1 IT 1998MI002491 A IT1998MI002491 A IT 1998MI002491A IT MI982491 A ITMI982491 A IT MI982491A IT 1303767 B1 IT1303767 B1 IT 1303767B1
- Authority
- IT
- Italy
- Prior art keywords
- nucleic acids
- quantification
- calibrator
- probes
- nucleic acid
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/702—Specific hybridization probes for retroviruses
- C12Q1/703—Viruses associated with AIDS
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/705—Specific hybridization probes for herpetoviridae, e.g. herpes simplex, varicella zoster
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Virology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- AIDS & HIV (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Priority Applications (11)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT1998MI002491A IT1303767B1 (it) | 1998-11-17 | 1998-11-17 | Metodo di quantificazione di acidi nucleici. |
| DE69936532T DE69936532D1 (de) | 1998-11-17 | 1999-11-17 | Verfahren zum quantitativen nachweis von nukleinsäuren |
| EP99956010A EP1131466B1 (en) | 1998-11-17 | 1999-11-17 | Method for the quantitative detection of nucleic acids |
| AT99956010T ATE366822T1 (de) | 1998-11-17 | 1999-11-17 | Verfahren zum quantitativen nachweis von nukleinsäuren |
| AU12720/00A AU1272000A (en) | 1998-11-17 | 1999-11-17 | Method for the quantitative detection of nucleic acids |
| US09/831,820 US7029843B1 (en) | 1998-11-17 | 1999-11-17 | Method for the quantitative detection of nucleic acids |
| PCT/EP1999/008847 WO2000029613A1 (en) | 1998-11-17 | 1999-11-17 | Method for the quantitative detection of nucleic acids |
| JP2000582594A JP2002530090A (ja) | 1998-11-17 | 1999-11-17 | 核酸の定量的検出方法 |
| CA002351740A CA2351740A1 (en) | 1998-11-17 | 1999-11-17 | Method for the quantitative detection of nucleic acids |
| US11/169,694 US7384769B2 (en) | 1998-11-17 | 2005-06-30 | Method for the quantitative detection of nucleic acids |
| US12/133,561 US8012719B2 (en) | 1998-11-17 | 2008-06-05 | Method for the quantitative detection of nucleic acids |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT1998MI002491A IT1303767B1 (it) | 1998-11-17 | 1998-11-17 | Metodo di quantificazione di acidi nucleici. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ITMI982491A1 ITMI982491A1 (it) | 2000-05-17 |
| IT1303767B1 true IT1303767B1 (it) | 2001-02-23 |
Family
ID=11381075
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IT1998MI002491A IT1303767B1 (it) | 1998-11-17 | 1998-11-17 | Metodo di quantificazione di acidi nucleici. |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US7029843B1 (it) |
| EP (1) | EP1131466B1 (it) |
| JP (1) | JP2002530090A (it) |
| AT (1) | ATE366822T1 (it) |
| AU (1) | AU1272000A (it) |
| CA (1) | CA2351740A1 (it) |
| DE (1) | DE69936532D1 (it) |
| IT (1) | IT1303767B1 (it) |
| WO (1) | WO2000029613A1 (it) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8012719B2 (en) * | 1998-11-17 | 2011-09-06 | Paolo Lusso | Method for the quantitative detection of nucleic acids |
| JP2002125687A (ja) * | 2000-10-30 | 2002-05-08 | Tosoh Corp | Ｈｉｖ−１検出のためのオリゴヌクレオチドおよび検出法 |
| AU2002237982A1 (en) * | 2001-01-30 | 2002-08-12 | Vertex Pharmaceuticals Incorporated | A quantitative assay for nucleic acids |
| EP1236804A1 (en) | 2001-03-02 | 2002-09-04 | Boehringer Mannheim Gmbh | A method for determination of a nucleic acid using a control |
| ES2305144T3 (es) * | 2001-03-02 | 2008-11-01 | F. Hoffmann-La Roche Ag | Metodo para la determinacion de un acido nucleico utilizando un control. |
| WO2003075837A2 (en) * | 2002-03-04 | 2003-09-18 | U.S. Army Medical Research And Materiel Command | Internal positive control for probe-based nucleic acid molecule assays and methods of making and using therof |
| US7052848B2 (en) | 2002-03-04 | 2006-05-30 | The United States Of America As Represented By The Secretary Of The Army | Internal positive control for probe-based nucleic acid molecule assays and methods of making and using thereof |
| EP2163651A1 (en) * | 2002-07-26 | 2010-03-17 | Abbott Laboratories | Method of detecting and quantifying hepatitis C virus |
| US7396646B2 (en) * | 2003-01-22 | 2008-07-08 | Modular Genetics, Inc. | Alien sequences |
| JP4805158B2 (ja) * | 2003-05-16 | 2011-11-02 | アメリカ合衆国 | 核酸増幅系に用いる内部コントロール核酸分子 |
| US8044184B2 (en) | 2004-04-26 | 2011-10-25 | Wako Pure Chemical Industries, Ltd. | Probe and primer for tubercle bacillus detection, and method of detecting human tubercle bacillus therewith |
| US20060110724A1 (en) | 2004-11-19 | 2006-05-25 | Burkhardt William Iii | Quantitative real-time assay for noroviruses and enteroviruses with built in quality control standard |
| JP2008182920A (ja) * | 2007-01-29 | 2008-08-14 | Sony Corp | 蛍光標識オリゴヌクレオチドと該オリゴヌクレオチドを利用する方法 |
| US20080274458A1 (en) * | 2007-05-01 | 2008-11-06 | Latham Gary J | Nucleic acid quantitation methods |
| US8366652B2 (en) | 2007-08-17 | 2013-02-05 | The Invention Science Fund I, Llc | Systems, devices, and methods including infection-fighting and monitoring shunts |
| JP5223267B2 (ja) * | 2007-08-23 | 2013-06-26 | 東レ株式会社 | 遺伝子増幅産物の回収方法および遺伝子増幅産物の回収キット |
| EP2141246A1 (en) * | 2008-07-01 | 2010-01-06 | Koninklijke Philips Electronics N.V. | Separation-free methods of PCR detection using SERRS |
| EP2384168B1 (en) | 2008-12-04 | 2014-10-08 | Searete LLC | Actively-controllable sterilizing excitation delivery implants |
| US20110295089A1 (en) | 2008-12-04 | 2011-12-01 | Searete Llc, A Limited Liability Corporation Of The State Of Delaware | Systems, devices, and methods including implantable devices with anti-microbial properties |
| WO2011002418A1 (en) * | 2009-07-03 | 2011-01-06 | Agency For Science, Technology And Research | Method and/or primers for the detection of mycobacterium tuberculosis |
| US8877464B2 (en) | 2010-07-29 | 2014-11-04 | Roche Molecular Systems, Inc. | Qualitative and quantitative detection of microbial nucleic acids |
| CN102154515A (zh) * | 2011-03-18 | 2011-08-17 | 武汉大学 | 人类疱疹病毒6型实时荧光定量pcr试剂盒 |
| EP2707496A1 (en) | 2011-05-11 | 2014-03-19 | Diagon Kft. | Procedure for rapid determination of viruses using nucleic acid-based molecular diagnostics, and a kit for this purpose |
| US9101678B2 (en) | 2011-11-03 | 2015-08-11 | Elwha Llc | Heat-sanitization of surfaces |
| CA2873370C (en) * | 2012-07-16 | 2021-06-08 | Kabushiki Kaisha Dnaform | Nucleic acid probe, method for designing nucleic acid probe, and method for detecting target sequence |
| JP5598784B2 (ja) | 2012-08-30 | 2014-10-01 | 株式会社ダナフォーム | 標的核酸の分析方法、キットおよび分析機器 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5457027A (en) | 1993-05-05 | 1995-10-10 | Becton, Dickinson And Company | Internal controls for isothermal nucleic acid amplification reactions |
| WO1995034684A1 (en) | 1994-06-10 | 1995-12-21 | Georgetown University | Recombinant virus and related method of quantitating same |
| US6395470B2 (en) * | 1997-10-31 | 2002-05-28 | Cenetron Diagnostics, Llc | Method for monitoring nucleic acid assays using synthetic internal controls with reversed nucleotide sequences |
-
1998
- 1998-11-17 IT IT1998MI002491A patent/IT1303767B1/it active
-
1999
- 1999-11-17 EP EP99956010A patent/EP1131466B1/en not_active Expired - Lifetime
- 1999-11-17 JP JP2000582594A patent/JP2002530090A/ja active Pending
- 1999-11-17 CA CA002351740A patent/CA2351740A1/en not_active Abandoned
- 1999-11-17 AT AT99956010T patent/ATE366822T1/de not_active IP Right Cessation
- 1999-11-17 AU AU12720/00A patent/AU1272000A/en not_active Abandoned
- 1999-11-17 DE DE69936532T patent/DE69936532D1/de not_active Expired - Lifetime
- 1999-11-17 US US09/831,820 patent/US7029843B1/en not_active Expired - Fee Related
- 1999-11-17 WO PCT/EP1999/008847 patent/WO2000029613A1/en active IP Right Grant
-
2005
- 2005-06-30 US US11/169,694 patent/US7384769B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| US7029843B1 (en) | 2006-04-18 |
| US20050239128A1 (en) | 2005-10-27 |
| ITMI982491A1 (it) | 2000-05-17 |
| CA2351740A1 (en) | 2000-05-25 |
| EP1131466B1 (en) | 2007-07-11 |
| DE69936532D1 (de) | 2007-08-23 |
| US7384769B2 (en) | 2008-06-10 |
| JP2002530090A (ja) | 2002-09-17 |
| WO2000029613A1 (en) | 2000-05-25 |
| ATE366822T1 (de) | 2007-08-15 |
| EP1131466A1 (en) | 2001-09-12 |
| AU1272000A (en) | 2000-06-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| IT1303767B1 (it) | Metodo di quantificazione di acidi nucleici. | |
| FI953445A0 (fi) | Menetelmä nukleiinihappojen herkkään osoitukseen | |
| ATE243759T1 (de) | Verfahren zur dämpfung der fluoreszene in lösung von fluoreszenzmarkierten oligonucleotidsonden | |
| ES2136137T3 (es) | Controles internos para reacciones de amplificacion isotermica de acidos nucleicos. | |
| CA2163587A1 (en) | Method for the particularly sensitive detection of nucleic acids | |
| ATE207543T1 (de) | Isotherme amplifikation von nukleinsäuren durch strangverdrängung | |
| ES8609472A1 (es) | Una sonda de deteccion de acidos nucleicos | |
| GB9707531D0 (en) | Method for detecting nucleic acids | |
| WO2003073067A3 (en) | Rna detection enzymes | |
| EP0272098A3 (en) | Method for amplification and detection of rna sequences | |
| EP1686191A3 (en) | Fluorometric method for monitoring and detecting nucleic acid amplification | |
| ATE203775T1 (de) | Methode zur detektion einer gezielten nukleinsäure | |
| DE69332666D1 (de) | Verfahren zur einfachen nukleotid-primer-verlängerung zum nachweis spezifischer allele und dafür geeigneter kit | |
| ES2081848T3 (es) | Composiciones a base de conjugados de una enzima y de una hidrazona o hidrazina de un acido nucleico, sondas y metodos. | |
| GB9510954D0 (en) | Detection of DNA damage | |
| CA2140331A1 (en) | Detecting and Amplifying Target Nucleic Acids Using Exonucleolytic Activity | |
| WO1997008531A3 (en) | Detection of hepatitis gb virus genotypes | |
| WO2001016374A3 (en) | Methods and reagents for in situ amplification | |
| AU1911395A (en) | Method, reagent and kit for the detection and amplification of nucleic acid sequences | |
| AU2001256220A1 (en) | Specific multiplex analysis of nucleic acids | |
| EP1170383A3 (en) | Oligonucleotides and method for detection of small round structured virus (SRSV) RNA | |
| WO1999055847A3 (en) | Enzymatic nucleic acids molecules which modulate the expressions and/or replication of hepatitis c virus | |
| WO2001029256A3 (en) | Cephalic pain susceptibility marker | |
| NO20004780D0 (no) | Nye overføringsmidler for nukleinsyrer, preparater inneholdende disse samt midlenes anvendelse | |
| IT1285298B1 (it) | Metodo di rivelazione di acidi nucleici per doppia ibridazione e trascrizione |