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IS8375A - Aðferð til að hreinsa prótín framleidd í plöntum - Google Patents

Aðferð til að hreinsa prótín framleidd í plöntum

Info

Publication number
IS8375A
IS8375A IS8375A IS8375A IS8375A IS 8375 A IS8375 A IS 8375A IS 8375 A IS8375 A IS 8375A IS 8375 A IS8375 A IS 8375A IS 8375 A IS8375 A IS 8375A
Authority
IS
Iceland
Prior art keywords
plants
proteins produced
purifying proteins
purifying
produced
Prior art date
Application number
IS8375A
Other languages
English (en)
Inventor
Mäntylä Einar
L βrus örvar Björn
Original Assignee
ORF Líftækni hf.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/IS2004/000010 external-priority patent/WO2005021762A2/en
Application filed by ORF Líftækni hf. filed Critical ORF Líftækni hf.
Publication of IS8375A publication Critical patent/IS8375A/is

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424Serine endopeptidases (3.4.21)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/06Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/20Fusion polypeptide containing a tag with affinity for a non-protein ligand

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
IS8375A 2003-08-27 2006-03-27 Aðferð til að hreinsa prótín framleidd í plöntum IS8375A (is)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US49793503P 2003-08-27 2003-08-27
IS6929 2003-08-27
PCT/IS2004/000010 WO2005021762A2 (en) 2003-08-27 2004-08-27 A process for proteolytic cleavage and purification of recombinant proteins produced in plants

Publications (1)

Publication Number Publication Date
IS8375A true IS8375A (is) 2006-03-27

Family

ID=36791759

Family Applications (1)

Application Number Title Priority Date Filing Date
IS8375A IS8375A (is) 2003-08-27 2006-03-27 Aðferð til að hreinsa prótín framleidd í plöntum

Country Status (2)

Country Link
CN (1) CN1842600B (is)
IS (1) IS8375A (is)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102174532B (zh) * 2011-01-19 2013-05-01 中国科学技术大学 家族3纤维素结合域在真核生物中作为重组蛋白质表达和纯化的亲和标签的应用
CN105483193B (zh) * 2015-11-24 2019-03-29 上海联合赛尔生物工程有限公司 从含酶切位点的融合蛋白中纯化蛋白的方法及试剂盒
CN110650972A (zh) * 2017-03-29 2020-01-03 耶路撒冷希伯来大学伊森姆研究发展有限公司 对感兴趣的剂的纯化
CN111620926B (zh) * 2019-02-28 2022-12-23 康码(上海)生物科技有限公司 一种将被多糖物质吸附的蛋白质释放到溶液中的方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6048715A (en) * 1988-07-08 2000-04-11 Univ. Of British Columbia Two-phase partition affinity separation system and affinity separated cell-containing composition

Also Published As

Publication number Publication date
CN1842600A (zh) 2006-10-04
CN1842600B (zh) 2012-05-16

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