HRP20131058T1 - Geni i postupci za poveä†anje otpornosti biljaka na bolesti - Google Patents
Geni i postupci za poveä†anje otpornosti biljaka na bolesti Download PDFInfo
- Publication number
- HRP20131058T1 HRP20131058T1 HRP20131058AT HRP20131058T HRP20131058T1 HR P20131058 T1 HRP20131058 T1 HR P20131058T1 HR P20131058A T HRP20131058A T HR P20131058AT HR P20131058 T HRP20131058 T HR P20131058T HR P20131058 T1 HRP20131058 T1 HR P20131058T1
- Authority
- HR
- Croatia
- Prior art keywords
- promoter
- plant
- nucleotide sequence
- seq
- cell
- Prior art date
Links
- 208000035240 Disease Resistance Diseases 0.000 title claims 2
- 108090000623 proteins and genes Proteins 0.000 title 1
- 241000196324 Embryophyta Species 0.000 claims 19
- 239000002773 nucleotide Substances 0.000 claims 7
- 125000003729 nucleotide group Chemical group 0.000 claims 7
- 241000207199 Citrus Species 0.000 claims 4
- 235000020971 citrus fruits Nutrition 0.000 claims 4
- 201000010099 disease Diseases 0.000 claims 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims 4
- 230000009261 transgenic effect Effects 0.000 claims 4
- 229920001184 polypeptide Polymers 0.000 claims 3
- 102000004196 processed proteins & peptides Human genes 0.000 claims 3
- 108090000765 processed proteins & peptides Proteins 0.000 claims 3
- 108010068086 Polyubiquitin Proteins 0.000 claims 2
- 102100037935 Polyubiquitin-C Human genes 0.000 claims 2
- 235000013399 edible fruits Nutrition 0.000 claims 2
- 235000017317 Fortunella Nutrition 0.000 claims 1
- 108700028146 Genetic Enhancer Elements Proteins 0.000 claims 1
- 241001299723 Limonia <Rutaceae> Species 0.000 claims 1
- 235000002319 Microcitrus Nutrition 0.000 claims 1
- 241000158764 Murraya Species 0.000 claims 1
- 241001523486 Poncirus Species 0.000 claims 1
- 241001093501 Rutaceae Species 0.000 claims 1
- 239000003623 enhancer Substances 0.000 claims 1
- 230000001172 regenerating effect Effects 0.000 claims 1
- 230000001131 transforming effect Effects 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8281—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for bacterial resistance
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Claims (14)
1. Konstrukt koji sadrži (i) nukleotidnu sekvencu prikazanu u SEQ ID NO: 1 i (ii) promotora koji djeluje u biljkama, naznačen time da je nukleotidna sekvenca funkcionalno povezana na promotor tako da ovaj eksprimira polipeptidnu sekvencu prikazanu u SEQ ID NO: 14.
2. Konstrukt prema zahtjevu 1, naznačen time da je promotor odabran od CaMV 35S promotora, poliubikvitin promotora, specifičnog tkivnog promotora, te preferiranog tkivnog promotora.
3. Stanica biljke naznačena time da sadrži konstrukt prema zahtjevu 1.
4. Postupak za povećanje otpornosti na bolest raka citrusa u biljci ili stanici, naznačen time da se sastoji od prekomjernog eksprimiranja nukleotidne sekvence prikazane u SEQ ID NO: 1.
5. Postupak za povećanje otpornosti na bolest raka citrusa u biljci ili stanici, naznačen time da se sastoji od
(a) transformiranja biljke ili stanice sa konstruktom koji sadrži nukleotidnu sekvencu prikazanu u SEQ ID NO: 1, funkcionalno povezanu na promotor koji je aktivan u stanicama biljke;
(b) regeneriranja biljke iz navedene transformirane biljke ili stanice;
(c) odabira biljke ili stanice koja ima povećanu otpornost na bolest raka citrusa u odnosu na kontrolnu biljku.
6. Postupak prema zahtjevu 5, naznačen time da promotor je konstitutivni promotor ili specifični tkivni promotor.
7. Postupak prema zahtjevu 6, naznačen time da je specifični tkivni promotor ksilem- specifični promotor, floem-specifični promotor, ili ksilem/floem-specifični promotor.
8. Postupak prema zahtjevu 5, naznačen time da je promotor funkcionalno povezan na pojačivač.
9. Postupak prema zahtjevu 5, naznačen time da je biljka član porodice Rutaceae.
10. Postupak prema zahtjevu 9, naznačen time da je navedena biljka odabrana iz roda Citrus, Poncirus, Fortunella, Murraya, Microcitrus, Limonia, i Eremocitrus.
11. Transgena biljka ili njeno potomstvo, voće, ili sjeme, navedena biljka, potomstvo, voće ili sjeme naznačena time da ima u svojem genomu uključuje
(i) nukleotidnu sekvencu koja kodira polipeptid otporan na bolest koji je prikazan u SEQ ID NO: 14, i
(ii) promotor koji djeluje u biljkama i koji je operativno povezan na nukleotidnu sekvencu,
pri čemu navedena biljka prekomjerno eksprimira polipeptid otporan na bolest.
12. Transgena biljka prema zahtjevu 11, naznačena time da je nukleotidna sekvenca jedna koja je prikazana u SEQ ID NO: 1.
13. Transgena biljka prema zahtjevu 11, naznačena time da dodatno ima u svoj genom uključenu (iii) sekvencu pojačivača koja je kondenzirana na promotor.
14. Transgena biljka prema bilo kojem zahtjevu od 11 do 13, naznačena time da je promotor odabran od CaMV 35S promotora, poliubikvitin promotora, specifičnog tkivnog promotora, te preferiranog tkivnog promotora.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US5145908P | 2008-05-08 | 2008-05-08 | |
PCT/IB2009/005842 WO2009136293A2 (en) | 2008-05-08 | 2009-05-08 | Genes and methods for increasing disease resistance in plants |
Publications (1)
Publication Number | Publication Date |
---|---|
HRP20131058T1 true HRP20131058T1 (hr) | 2014-06-06 |
Family
ID=41265093
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
HRP20131058AT HRP20131058T1 (hr) | 2008-05-08 | 2013-11-07 | Geni i postupci za poveä†anje otpornosti biljaka na bolesti |
Country Status (9)
Country | Link |
---|---|
US (1) | US9051580B2 (hr) |
EP (2) | EP2283034B1 (hr) |
BR (1) | BRPI0912531A8 (hr) |
DK (1) | DK2283034T3 (hr) |
ES (2) | ES2584325T3 (hr) |
HR (1) | HRP20131058T1 (hr) |
PL (1) | PL2283034T3 (hr) |
SI (1) | SI2283034T1 (hr) |
WO (1) | WO2009136293A2 (hr) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BR102012008162B1 (pt) * | 2012-04-09 | 2018-08-14 | Empresa Brasileira De Pesquisa Agropecuária - Embrapa | composições e métodos para modificar a expressão de genes de interesse |
CN103882031B (zh) * | 2014-01-03 | 2016-08-24 | 上海交通大学 | 柑橘溃疡病感病基因CsLOB |
Family Cites Families (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4987071A (en) | 1986-12-03 | 1991-01-22 | University Patents, Inc. | RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods |
US5316931A (en) | 1988-02-26 | 1994-05-31 | Biosource Genetics Corp. | Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes |
US5496938A (en) | 1990-06-11 | 1996-03-05 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligands to HIV-RT and HIV-1 rev |
US5622854A (en) | 1992-05-14 | 1997-04-22 | Ribozyme Pharmaceuticals Inc. | Method and reagent for inhibiting T-cell leukemia virus replication |
US5583032A (en) | 1992-10-21 | 1996-12-10 | The Cleveland Clinic Foundation And National Institutes Of Health | Method of cleaving specific strands of RNA |
US5591601A (en) | 1993-05-14 | 1997-01-07 | Ohio University Edison Animal Biotechnology Institute | DNA polymerase gene expression system utilizing an RNA polymerase co-delivered with the gene expression vector system |
US5559021A (en) | 1993-11-09 | 1996-09-24 | Synaptic Pharmaceutical Corporation | DNA encoding a novel mammalian transporter homologous to neurotransmitter transporters and uses thereof |
US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
US5580967A (en) | 1994-05-13 | 1996-12-03 | The Scripps Research Institute | Optimized catalytic DNA-cleaving ribozymes |
AU2786100A (en) | 1999-02-26 | 2000-09-21 | Cropdesign N.V. | Method of selecting transformed cells and tissues |
US6723896B1 (en) | 1999-11-12 | 2004-04-20 | The Rockefeller University | Inducible site-specific recombination for the activation and removal of transgenes in transgenic plants |
US6506963B1 (en) | 1999-12-08 | 2003-01-14 | Plant Genetic Systems, N.V. | Hybrid winter oilseed rape and methods for producing same |
AU784571B2 (en) | 2000-02-08 | 2006-05-04 | Sakata Seed Corporation | Novel methods and constructs for plant transformation |
US6613960B1 (en) | 2000-02-15 | 2003-09-02 | Cornell Research Foundation, Inc. | Phloem-loading-specific promoter |
JPWO2002055689A1 (ja) | 2001-01-05 | 2004-05-20 | 日本たばこ産業株式会社 | イネスクローストランスポーター遺伝子プロモーター |
WO2003068911A2 (en) * | 2001-11-15 | 2003-08-21 | University Of Florida | Citrus tristeza virus resistance genes and methods of use |
DE102004043207C5 (de) | 2004-09-03 | 2010-09-09 | Südzucker AG Mannheim/Ochsenfurt | Wurzel- und xylemparenchymspezifischer Promotor |
WO2007137075A2 (en) | 2006-05-16 | 2007-11-29 | Monsanto Technology Llc | Use of non-agrobacterium bacterial species for plant transformation |
-
2009
- 2009-05-08 EP EP09742466.7A patent/EP2283034B1/en not_active Not-in-force
- 2009-05-08 PL PL09742466T patent/PL2283034T3/pl unknown
- 2009-05-08 ES ES13179392.9T patent/ES2584325T3/es active Active
- 2009-05-08 SI SI200930763T patent/SI2283034T1/sl unknown
- 2009-05-08 WO PCT/IB2009/005842 patent/WO2009136293A2/en active Application Filing
- 2009-05-08 BR BRPI0912531A patent/BRPI0912531A8/pt not_active Application Discontinuation
- 2009-05-08 DK DK09742466.7T patent/DK2283034T3/da active
- 2009-05-08 ES ES09742466T patent/ES2434742T3/es active Active
- 2009-05-08 US US12/991,412 patent/US9051580B2/en active Active
- 2009-05-08 EP EP13179392.9A patent/EP2682403B1/en not_active Not-in-force
-
2013
- 2013-11-07 HR HRP20131058AT patent/HRP20131058T1/hr unknown
Also Published As
Publication number | Publication date |
---|---|
EP2283034A2 (en) | 2011-02-16 |
BRPI0912531A8 (pt) | 2017-01-10 |
ES2584325T3 (es) | 2016-09-27 |
WO2009136293A3 (en) | 2010-08-26 |
EP2283034B1 (en) | 2013-08-07 |
BRPI0912531A2 (pt) | 2016-08-09 |
EP2682403B1 (en) | 2016-04-20 |
SI2283034T1 (sl) | 2015-07-31 |
US20110107459A1 (en) | 2011-05-05 |
EP2682403A3 (en) | 2014-04-02 |
DK2283034T3 (da) | 2013-11-18 |
EP2682403A2 (en) | 2014-01-08 |
WO2009136293A2 (en) | 2009-11-12 |
ES2434742T3 (es) | 2013-12-17 |
PL2283034T3 (pl) | 2014-05-30 |
US9051580B2 (en) | 2015-06-09 |
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