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HRP20131058T1 - Geni i postupci za poveä†anje otpornosti biljaka na bolesti - Google Patents

Geni i postupci za poveä†anje otpornosti biljaka na bolesti Download PDF

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Publication number
HRP20131058T1
HRP20131058T1 HRP20131058AT HRP20131058T HRP20131058T1 HR P20131058 T1 HRP20131058 T1 HR P20131058T1 HR P20131058A T HRP20131058A T HR P20131058AT HR P20131058 T HRP20131058 T HR P20131058T HR P20131058 T1 HRP20131058 T1 HR P20131058T1
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HR
Croatia
Prior art keywords
promoter
plant
nucleotide sequence
seq
cell
Prior art date
Application number
HRP20131058AT
Other languages
English (en)
Inventor
Ana Claudia Rasera Silva
Gustavo Adolfo Astua Monge
Original Assignee
Monsanto Do Brasil Ltda
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Do Brasil Ltda filed Critical Monsanto Do Brasil Ltda
Publication of HRP20131058T1 publication Critical patent/HRP20131058T1/hr

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8281Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for bacterial resistance
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Botany (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Claims (14)

1. Konstrukt koji sadrži (i) nukleotidnu sekvencu prikazanu u SEQ ID NO: 1 i (ii) promotora koji djeluje u biljkama, naznačen time da je nukleotidna sekvenca funkcionalno povezana na promotor tako da ovaj eksprimira polipeptidnu sekvencu prikazanu u SEQ ID NO: 14.
2. Konstrukt prema zahtjevu 1, naznačen time da je promotor odabran od CaMV 35S promotora, poliubikvitin promotora, specifičnog tkivnog promotora, te preferiranog tkivnog promotora.
3. Stanica biljke naznačena time da sadrži konstrukt prema zahtjevu 1.
4. Postupak za povećanje otpornosti na bolest raka citrusa u biljci ili stanici, naznačen time da se sastoji od prekomjernog eksprimiranja nukleotidne sekvence prikazane u SEQ ID NO: 1.
5. Postupak za povećanje otpornosti na bolest raka citrusa u biljci ili stanici, naznačen time da se sastoji od (a) transformiranja biljke ili stanice sa konstruktom koji sadrži nukleotidnu sekvencu prikazanu u SEQ ID NO: 1, funkcionalno povezanu na promotor koji je aktivan u stanicama biljke; (b) regeneriranja biljke iz navedene transformirane biljke ili stanice; (c) odabira biljke ili stanice koja ima povećanu otpornost na bolest raka citrusa u odnosu na kontrolnu biljku.
6. Postupak prema zahtjevu 5, naznačen time da promotor je konstitutivni promotor ili specifični tkivni promotor.
7. Postupak prema zahtjevu 6, naznačen time da je specifični tkivni promotor ksilem- specifični promotor, floem-specifični promotor, ili ksilem/floem-specifični promotor.
8. Postupak prema zahtjevu 5, naznačen time da je promotor funkcionalno povezan na pojačivač.
9. Postupak prema zahtjevu 5, naznačen time da je biljka član porodice Rutaceae.
10. Postupak prema zahtjevu 9, naznačen time da je navedena biljka odabrana iz roda Citrus, Poncirus, Fortunella, Murraya, Microcitrus, Limonia, i Eremocitrus.
11. Transgena biljka ili njeno potomstvo, voće, ili sjeme, navedena biljka, potomstvo, voće ili sjeme naznačena time da ima u svojem genomu uključuje (i) nukleotidnu sekvencu koja kodira polipeptid otporan na bolest koji je prikazan u SEQ ID NO: 14, i (ii) promotor koji djeluje u biljkama i koji je operativno povezan na nukleotidnu sekvencu, pri čemu navedena biljka prekomjerno eksprimira polipeptid otporan na bolest.
12. Transgena biljka prema zahtjevu 11, naznačena time da je nukleotidna sekvenca jedna koja je prikazana u SEQ ID NO: 1.
13. Transgena biljka prema zahtjevu 11, naznačena time da dodatno ima u svoj genom uključenu (iii) sekvencu pojačivača koja je kondenzirana na promotor.
14. Transgena biljka prema bilo kojem zahtjevu od 11 do 13, naznačena time da je promotor odabran od CaMV 35S promotora, poliubikvitin promotora, specifičnog tkivnog promotora, te preferiranog tkivnog promotora.
HRP20131058AT 2008-05-08 2013-11-07 Geni i postupci za poveä†anje otpornosti biljaka na bolesti HRP20131058T1 (hr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US5145908P 2008-05-08 2008-05-08
PCT/IB2009/005842 WO2009136293A2 (en) 2008-05-08 2009-05-08 Genes and methods for increasing disease resistance in plants

Publications (1)

Publication Number Publication Date
HRP20131058T1 true HRP20131058T1 (hr) 2014-06-06

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
HRP20131058AT HRP20131058T1 (hr) 2008-05-08 2013-11-07 Geni i postupci za poveä†anje otpornosti biljaka na bolesti

Country Status (9)

Country Link
US (1) US9051580B2 (hr)
EP (2) EP2283034B1 (hr)
BR (1) BRPI0912531A8 (hr)
DK (1) DK2283034T3 (hr)
ES (2) ES2584325T3 (hr)
HR (1) HRP20131058T1 (hr)
PL (1) PL2283034T3 (hr)
SI (1) SI2283034T1 (hr)
WO (1) WO2009136293A2 (hr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR102012008162B1 (pt) * 2012-04-09 2018-08-14 Empresa Brasileira De Pesquisa Agropecuária - Embrapa composições e métodos para modificar a expressão de genes de interesse
CN103882031B (zh) * 2014-01-03 2016-08-24 上海交通大学 柑橘溃疡病感病基因CsLOB

Family Cites Families (18)

* Cited by examiner, † Cited by third party
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US4987071A (en) 1986-12-03 1991-01-22 University Patents, Inc. RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods
US5316931A (en) 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US5496938A (en) 1990-06-11 1996-03-05 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands to HIV-RT and HIV-1 rev
US5622854A (en) 1992-05-14 1997-04-22 Ribozyme Pharmaceuticals Inc. Method and reagent for inhibiting T-cell leukemia virus replication
US5583032A (en) 1992-10-21 1996-12-10 The Cleveland Clinic Foundation And National Institutes Of Health Method of cleaving specific strands of RNA
US5591601A (en) 1993-05-14 1997-01-07 Ohio University Edison Animal Biotechnology Institute DNA polymerase gene expression system utilizing an RNA polymerase co-delivered with the gene expression vector system
US5559021A (en) 1993-11-09 1996-09-24 Synaptic Pharmaceutical Corporation DNA encoding a novel mammalian transporter homologous to neurotransmitter transporters and uses thereof
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5580967A (en) 1994-05-13 1996-12-03 The Scripps Research Institute Optimized catalytic DNA-cleaving ribozymes
AU2786100A (en) 1999-02-26 2000-09-21 Cropdesign N.V. Method of selecting transformed cells and tissues
US6723896B1 (en) 1999-11-12 2004-04-20 The Rockefeller University Inducible site-specific recombination for the activation and removal of transgenes in transgenic plants
US6506963B1 (en) 1999-12-08 2003-01-14 Plant Genetic Systems, N.V. Hybrid winter oilseed rape and methods for producing same
AU784571B2 (en) 2000-02-08 2006-05-04 Sakata Seed Corporation Novel methods and constructs for plant transformation
US6613960B1 (en) 2000-02-15 2003-09-02 Cornell Research Foundation, Inc. Phloem-loading-specific promoter
JPWO2002055689A1 (ja) 2001-01-05 2004-05-20 日本たばこ産業株式会社 イネスクローストランスポーター遺伝子プロモーター
WO2003068911A2 (en) * 2001-11-15 2003-08-21 University Of Florida Citrus tristeza virus resistance genes and methods of use
DE102004043207C5 (de) 2004-09-03 2010-09-09 Südzucker AG Mannheim/Ochsenfurt Wurzel- und xylemparenchymspezifischer Promotor
WO2007137075A2 (en) 2006-05-16 2007-11-29 Monsanto Technology Llc Use of non-agrobacterium bacterial species for plant transformation

Also Published As

Publication number Publication date
EP2283034A2 (en) 2011-02-16
BRPI0912531A8 (pt) 2017-01-10
ES2584325T3 (es) 2016-09-27
WO2009136293A3 (en) 2010-08-26
EP2283034B1 (en) 2013-08-07
BRPI0912531A2 (pt) 2016-08-09
EP2682403B1 (en) 2016-04-20
SI2283034T1 (sl) 2015-07-31
US20110107459A1 (en) 2011-05-05
EP2682403A3 (en) 2014-04-02
DK2283034T3 (da) 2013-11-18
EP2682403A2 (en) 2014-01-08
WO2009136293A2 (en) 2009-11-12
ES2434742T3 (es) 2013-12-17
PL2283034T3 (pl) 2014-05-30
US9051580B2 (en) 2015-06-09

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