HK1199891A1 - Rapid method for cloning and expression of cognate antibody variable region gene segments - Google Patents
Rapid method for cloning and expression of cognate antibody variable region gene segmentsInfo
- Publication number
- HK1199891A1 HK1199891A1 HK15100396.3A HK15100396A HK1199891A1 HK 1199891 A1 HK1199891 A1 HK 1199891A1 HK 15100396 A HK15100396 A HK 15100396A HK 1199891 A1 HK1199891 A1 HK 1199891A1
- Authority
- HK
- Hong Kong
- Prior art keywords
- cloning
- expression
- variable region
- gene segments
- antibody variable
- Prior art date
Links
- 238000010367 cloning Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0635—B lymphocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Analytical Chemistry (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP11194861 | 2011-12-21 | ||
| PCT/EP2012/076155 WO2013092716A1 (en) | 2011-12-21 | 2012-12-19 | Rapid method for cloning and expression of cognate antibody variable region gene segments |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HK1199891A1 true HK1199891A1 (en) | 2015-07-24 |
Family
ID=47522537
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HK15100396.3A HK1199891A1 (en) | 2011-12-21 | 2015-01-14 | Rapid method for cloning and expression of cognate antibody variable region gene segments |
Country Status (12)
| Country | Link |
|---|---|
| US (3) | US20140302559A1 (xx) |
| EP (1) | EP2794652B1 (xx) |
| JP (3) | JP2015500655A (xx) |
| KR (1) | KR20140104963A (xx) |
| CN (2) | CN104011074A (xx) |
| BR (1) | BR112014013034A2 (xx) |
| CA (1) | CA2854243A1 (xx) |
| HK (1) | HK1199891A1 (xx) |
| MX (1) | MX354049B (xx) |
| RU (1) | RU2612910C2 (xx) |
| SG (1) | SG11201403365TA (xx) |
| WO (1) | WO2013092716A1 (xx) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PT2417156E (pt) | 2009-04-07 | 2015-04-29 | Roche Glycart Ag | Anticorpos trivalentes, biespecíficos |
| US9676845B2 (en) | 2009-06-16 | 2017-06-13 | Hoffmann-La Roche, Inc. | Bispecific antigen binding proteins |
| RU2015153109A (ru) | 2009-09-16 | 2019-01-15 | Дженентек, Инк. | Содержащие суперспираль и/или привязку белковые комплексы и их применения |
| AR080793A1 (es) | 2010-03-26 | 2012-05-09 | Roche Glycart Ag | Anticuerpos biespecificos |
| KR101586128B1 (ko) | 2010-08-24 | 2016-01-15 | 에프. 호프만-라 로슈 아게 | 디술피드 안정화 ― Ｆｖ 단편을 포함하는 이중특이적 항체 |
| JP5766296B2 (ja) | 2010-12-23 | 2015-08-19 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | ポリペプチド−ポリヌクレオチド複合体、およびエフェクター成分の標的化された送達におけるその使用 |
| AR085403A1 (es) | 2011-02-28 | 2013-09-25 | Hoffmann La Roche | Proteinas monovalentes que se unen a antigenos |
| CA2825081A1 (en) | 2011-02-28 | 2012-09-07 | Birgit Bossenmaier | Antigen binding proteins |
| CN104011074A (zh) * | 2011-12-21 | 2014-08-27 | 弗·哈夫曼-拉罗切有限公司 | 用于克隆和表达关联抗体可变区基因区段的快速方法 |
| JP6486686B2 (ja) | 2012-02-10 | 2019-03-20 | ジェネンテック， インコーポレイテッド | 単鎖抗体及び他のヘテロ多量体 |
| WO2014001324A1 (en) | 2012-06-27 | 2014-01-03 | Hoffmann-La Roche Ag | Method for selection and production of tailor-made highly selective and multi-specific targeting entities containing at least two different binding entities and uses thereof |
| BR112014028368A2 (pt) | 2012-06-27 | 2017-11-14 | Hoffmann La Roche | método de produção de conjugado de região fc de anticorpo, conjugado de região fc de anticorpo e formulação farmacêutica |
| WO2015000624A1 (en) * | 2014-05-19 | 2015-01-08 | F. Hoffmann-La Roche Ag | Method for producing antibodies using ovine b-cells and uses thereof |
| US20160075766A1 (en) * | 2013-05-21 | 2016-03-17 | Roche Diagnostics Operations, Inc. | Method for producing antibodies using ovine b-cells and uses thereof |
| CA2922912A1 (en) | 2013-10-11 | 2015-04-16 | F. Hoffmann-La Roche Ag | Multispecific domain exchanged common variable light chain antibodies |
| WO2016087416A1 (en) | 2014-12-03 | 2016-06-09 | F. Hoffmann-La Roche Ag | Multispecific antibodies |
| WO2017167714A1 (en) | 2016-03-30 | 2017-10-05 | F. Hoffmann-La Roche Ag | B-cell cultivation method |
| KR20240133790A (ko) * | 2016-12-23 | 2024-09-04 | 비스테라, 인크. | 결합 폴리펩타이드 및 이의 제조 방법 |
| CN110121554B (zh) | 2017-01-02 | 2023-08-15 | 豪夫迈·罗氏有限公司 | B细胞培养方法 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9815909D0 (en) * | 1998-07-21 | 1998-09-16 | Btg Int Ltd | Antibody preparation |
| CN100383244C (zh) * | 2003-01-07 | 2008-04-23 | 西福根有限公司 | 用于生产重组多克隆蛋白质的方法 |
| US20060051348A1 (en) | 2004-09-09 | 2006-03-09 | Jorn Gorlach | Method of producing a plurality of isolated antibodies to a plurality of cognate antigens |
| US8642513B2 (en) | 2005-09-15 | 2014-02-04 | Crucell Holland B.V. | Method for preparing immunoglobulin libraries |
| US20070269868A1 (en) | 2006-05-19 | 2007-11-22 | Carvalho Jensen Anne E | Culture method for obtaining a clonal population of antigen-specific B cells |
| PL3187506T3 (pl) | 2007-05-21 | 2019-09-30 | Alderbio Holdings Llc | Przeciwciała przeciwko IL-6 i ich zastosowanie |
| CN102272158B (zh) * | 2008-11-12 | 2014-05-07 | 乔治·K·刘易斯 | 源自记忆b细胞的人单克隆抗体的快速表达克隆 |
| US8609097B2 (en) * | 2009-06-10 | 2013-12-17 | Hoffmann-La Roche Inc. | Use of an anti-Tau pS422 antibody for the treatment of brain diseases |
| EP2524041A4 (en) * | 2009-11-16 | 2013-08-07 | Acyte Biotech Pty Ltd | RECOMBINANT ANTIBODY VECTOR |
| WO2011112566A2 (en) * | 2010-03-11 | 2011-09-15 | Abbott Laboratories | Basigin binding proteins |
| DK2400298T3 (da) | 2010-05-28 | 2013-09-02 | Hoffmann La Roche | Enkelt B-celle-dyrkningsfremgangsmåde og specifik antistoffremstilling |
| CN104011074A (zh) * | 2011-12-21 | 2014-08-27 | 弗·哈夫曼-拉罗切有限公司 | 用于克隆和表达关联抗体可变区基因区段的快速方法 |
-
2012
- 2012-12-19 CN CN201280060807.3A patent/CN104011074A/zh active Pending
- 2012-12-19 CA CA2854243A patent/CA2854243A1/en not_active Abandoned
- 2012-12-19 KR KR1020147016436A patent/KR20140104963A/ko not_active Application Discontinuation
- 2012-12-19 RU RU2014128987A patent/RU2612910C2/ru not_active IP Right Cessation
- 2012-12-19 MX MX2014007256A patent/MX354049B/es active IP Right Grant
- 2012-12-19 BR BR112014013034A patent/BR112014013034A2/pt active Search and Examination
- 2012-12-19 SG SG11201403365TA patent/SG11201403365TA/en unknown
- 2012-12-19 EP EP12812945.9A patent/EP2794652B1/en active Active
- 2012-12-19 CN CN201910132084.7A patent/CN109810188A/zh active Pending
- 2012-12-19 JP JP2014547976A patent/JP2015500655A/ja active Pending
- 2012-12-19 WO PCT/EP2012/076155 patent/WO2013092716A1/en active Application Filing
-
2014
- 2014-06-20 US US14/310,966 patent/US20140302559A1/en not_active Abandoned
-
2015
- 2015-01-14 HK HK15100396.3A patent/HK1199891A1/xx unknown
-
2017
- 2017-04-06 US US15/480,533 patent/US20170210785A1/en not_active Abandoned
- 2017-06-14 JP JP2017116638A patent/JP6573331B2/ja active Active
-
2019
- 2019-01-10 US US16/244,909 patent/US12024548B2/en active Active
- 2019-06-03 JP JP2019103602A patent/JP2019162144A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP2794652A1 (en) | 2014-10-29 |
| JP6573331B2 (ja) | 2019-09-11 |
| WO2013092716A1 (en) | 2013-06-27 |
| JP2017221190A (ja) | 2017-12-21 |
| EP2794652B1 (en) | 2017-11-15 |
| US12024548B2 (en) | 2024-07-02 |
| RU2014128987A (ru) | 2016-02-20 |
| US20190382469A1 (en) | 2019-12-19 |
| CN104011074A (zh) | 2014-08-27 |
| SG11201403365TA (en) | 2014-07-30 |
| MX2014007256A (es) | 2014-08-01 |
| RU2612910C2 (ru) | 2017-03-13 |
| CN109810188A (zh) | 2019-05-28 |
| MX354049B (es) | 2018-02-09 |
| US20170210785A1 (en) | 2017-07-27 |
| JP2015500655A (ja) | 2015-01-08 |
| JP2019162144A (ja) | 2019-09-26 |
| KR20140104963A (ko) | 2014-08-29 |
| CA2854243A1 (en) | 2013-06-27 |
| BR112014013034A2 (pt) | 2017-12-19 |
| US20140302559A1 (en) | 2014-10-09 |
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