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HK1198336A1 - Method for large-scale synthesis of long-chain nucleic acid molecule - Google Patents

Method for large-scale synthesis of long-chain nucleic acid molecule

Info

Publication number
HK1198336A1
HK1198336A1 HK14111273.9A HK14111273A HK1198336A1 HK 1198336 A1 HK1198336 A1 HK 1198336A1 HK 14111273 A HK14111273 A HK 14111273A HK 1198336 A1 HK1198336 A1 HK 1198336A1
Authority
HK
Hong Kong
Prior art keywords
long
nucleic acid
acid molecule
chain nucleic
scale synthesis
Prior art date
Application number
HK14111273.9A
Other languages
English (en)
Chinese (zh)
Inventor
馬千君
李紹路
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed filed Critical
Publication of HK1198336A1 publication Critical patent/HK1198336A1/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
HK14111273.9A 2011-07-12 2014-11-06 Method for large-scale synthesis of long-chain nucleic acid molecule HK1198336A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN2011101936354A CN102876658A (zh) 2011-07-12 2011-07-12 一种大规模合成长链核酸分子的方法
PCT/CN2012/000818 WO2013007099A1 (fr) 2011-07-12 2012-06-13 Procédé de synthèse à grande échelle d'une molécule d'acide nucléique à chaîne longue

Publications (1)

Publication Number Publication Date
HK1198336A1 true HK1198336A1 (en) 2015-06-19

Family

ID=47478177

Family Applications (1)

Application Number Title Priority Date Filing Date
HK14111273.9A HK1198336A1 (en) 2011-07-12 2014-11-06 Method for large-scale synthesis of long-chain nucleic acid molecule

Country Status (6)

Country Link
US (1) US9695417B2 (fr)
EP (1) EP2733208B1 (fr)
JP (1) JP6069314B2 (fr)
CN (1) CN102876658A (fr)
HK (1) HK1198336A1 (fr)
WO (1) WO2013007099A1 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102584955A (zh) * 2011-12-31 2012-07-18 北京唯尚立德生物科技有限公司 一种转录激活子样效应因子的固相合成方法
AU2018350212A1 (en) 2017-10-13 2020-04-23 Ribbon Biolabs Gmbh A novel method for synthesis of polynucleotides using a diverse library of oligonucleotides
CN109825431A (zh) * 2019-03-18 2019-05-31 中国人民解放军军事科学院军事医学研究院 寡核苷酸原位组装仪
CN113041967B (zh) * 2021-02-26 2022-09-27 通用生物(安徽)股份有限公司 一种超长链核酸合成生产工艺

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6479262B1 (en) * 2000-05-16 2002-11-12 Hercules, Incorporated Solid phase enzymatic assembly of polynucleotides
US7575865B2 (en) * 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
CN101133166B (zh) * 2005-03-10 2011-02-02 高晓莲 平行制备核酸及应用
US9012184B2 (en) * 2006-02-08 2015-04-21 Illumina Cambridge Limited End modification to prevent over-representation of fragments

Also Published As

Publication number Publication date
US20140221255A1 (en) 2014-08-07
EP2733208A4 (fr) 2015-02-25
EP2733208A1 (fr) 2014-05-21
JP6069314B2 (ja) 2017-02-01
US9695417B2 (en) 2017-07-04
WO2013007099A1 (fr) 2013-01-17
EP2733208B1 (fr) 2017-04-05
CN102876658A (zh) 2013-01-16
JP2014521312A (ja) 2014-08-28

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