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GB9324955D0 - Protecting nucleic acids and methods of analysis - Google Patents

Protecting nucleic acids and methods of analysis

Info

Publication number
GB9324955D0
GB9324955D0 GB939324955A GB9324955A GB9324955D0 GB 9324955 D0 GB9324955 D0 GB 9324955D0 GB 939324955 A GB939324955 A GB 939324955A GB 9324955 A GB9324955 A GB 9324955A GB 9324955 D0 GB9324955 D0 GB 9324955D0
Authority
GB
United Kingdom
Prior art keywords
nucleic acid
analysis
methods
nucleic acids
protecting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB939324955A
Other versions
GB2285445A (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
PNA Diagnostics ApS Denmark
Original Assignee
PNA Diagnostics ApS Denmark
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by PNA Diagnostics ApS Denmark filed Critical PNA Diagnostics ApS Denmark
Priority to GB9324955A priority Critical patent/GB2285445A/en
Publication of GB9324955D0 publication Critical patent/GB9324955D0/en
Priority to EP95903782A priority patent/EP0733062B1/en
Priority to JP7515942A priority patent/JPH09506255A/en
Priority to KR1019960703043A priority patent/KR100221399B1/en
Priority to DE69410291T priority patent/DE69410291T2/en
Priority to ES95903782T priority patent/ES2119370T3/en
Priority to AU12729/95A priority patent/AU682563B2/en
Priority to CA002177603A priority patent/CA2177603C/en
Priority to DK95903782T priority patent/DK0733062T3/en
Priority to PCT/EP1994/003973 priority patent/WO1995015974A1/en
Priority to AT95903782T priority patent/ATE166066T1/en
Priority to IL11183194A priority patent/IL111831A/en
Priority to ZA949643A priority patent/ZA949643B/en
Publication of GB2285445A publication Critical patent/GB2285445A/en
Priority to NO19962331A priority patent/NO317934B1/en
Priority to FI962340A priority patent/FI111266B/en
Priority to US08/659,529 priority patent/US5861250A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
    • C07K14/003—Peptide-nucleic acids (PNAs)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

A selected region of a nucleic acid is protected from attach by nuclease by completing to the nucleic acid a nucleic acid analogue of the PNA type. The surviving sequence may be detected in an assay, optionally after amplification. A PCR reaction may be sterilized and its product assayed by protecting a characterizing region of the product by PNA hybridization followed by nuclease degradation of unprotected nucleic acid.
GB9324955A 1993-12-06 1993-12-06 Protecting nucleic acids and methods of analysis Withdrawn GB2285445A (en)

Priority Applications (16)

Application Number Priority Date Filing Date Title
GB9324955A GB2285445A (en) 1993-12-06 1993-12-06 Protecting nucleic acids and methods of analysis
IL11183194A IL111831A (en) 1993-12-06 1994-11-30 Method of detecting the presence of a nucleic acid in a sample
DK95903782T DK0733062T3 (en) 1993-12-06 1994-11-30 Protection of nucleic acids and method of analysis
AT95903782T ATE166066T1 (en) 1993-12-06 1994-11-30 PROTECTION OF NUCLEIC ACIDS AND METHOD FOR ANALYSIS
KR1019960703043A KR100221399B1 (en) 1993-12-06 1994-11-30 Protecting nucleic acids and method of analysis
DE69410291T DE69410291T2 (en) 1993-12-06 1994-11-30 PROTECTION OF NUCLEIC ACIDS AND METHOD FOR ANALYSIS
ES95903782T ES2119370T3 (en) 1993-12-06 1994-11-30 PROTECTION OF NUCLEIC ACIDS AND METHODS OF ANALYSIS.
AU12729/95A AU682563B2 (en) 1993-12-06 1994-11-30 Protecting nucleic acids and methods of analysis
CA002177603A CA2177603C (en) 1993-12-06 1994-11-30 Protecting nucleic acids and methods of analysis
EP95903782A EP0733062B1 (en) 1993-12-06 1994-11-30 Protecting nucleic acids and methods of analysis
PCT/EP1994/003973 WO1995015974A1 (en) 1993-12-06 1994-11-30 Protecting nucleic acids and methods of analysis
JP7515942A JPH09506255A (en) 1993-12-06 1994-11-30 Nucleic acid protection method and analysis method
ZA949643A ZA949643B (en) 1993-12-06 1994-12-05 Protecting nucleic acids and methods of analysis
NO19962331A NO317934B1 (en) 1993-12-06 1996-06-05 Method of protecting and mapping a nucleic acid sequence using a nucleic acid analog, method of performing a nucleic acid amplification, and method of working up reaction product thereof.
FI962340A FI111266B (en) 1993-12-06 1996-06-05 Protection of nucleic acids and methods of analysis
US08/659,529 US5861250A (en) 1993-12-06 1996-06-06 Protecting nucleic acids and methods of analysis

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB9324955A GB2285445A (en) 1993-12-06 1993-12-06 Protecting nucleic acids and methods of analysis

Publications (2)

Publication Number Publication Date
GB9324955D0 true GB9324955D0 (en) 1994-01-26
GB2285445A GB2285445A (en) 1995-07-12

Family

ID=10746180

Family Applications (1)

Application Number Title Priority Date Filing Date
GB9324955A Withdrawn GB2285445A (en) 1993-12-06 1993-12-06 Protecting nucleic acids and methods of analysis

Country Status (16)

Country Link
US (1) US5861250A (en)
EP (1) EP0733062B1 (en)
JP (1) JPH09506255A (en)
KR (1) KR100221399B1 (en)
AT (1) ATE166066T1 (en)
AU (1) AU682563B2 (en)
CA (1) CA2177603C (en)
DE (1) DE69410291T2 (en)
DK (1) DK0733062T3 (en)
ES (1) ES2119370T3 (en)
FI (1) FI111266B (en)
GB (1) GB2285445A (en)
IL (1) IL111831A (en)
NO (1) NO317934B1 (en)
WO (1) WO1995015974A1 (en)
ZA (1) ZA949643B (en)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996027679A1 (en) 1995-03-04 1996-09-12 Boehringer Mannheim Gmbh Modulation of the binding properties of nucleic acid binding partners
WO1996012397A2 (en) * 1995-06-22 1996-05-02 Dako A/S Recombinant antibody capable of binding to pna/nucleic acid complexes
AU4327196A (en) * 1995-06-22 1996-05-31 Dako A/S Monoclonal antibody capable of binding to pna/nucleic acid complexes
US5677124A (en) * 1996-07-03 1997-10-14 Ambion, Inc. Ribonuclease resistant viral RNA standards
US5939262A (en) * 1996-07-03 1999-08-17 Ambion, Inc. Ribonuclease resistant RNA preparation and utilization
US5853990A (en) 1996-07-26 1998-12-29 Edward E. Winger Real time homogeneous nucleotide assay
DE19633436A1 (en) 1996-08-20 1998-02-26 Boehringer Mannheim Gmbh Method for the detection of nucleic acids by determining the mass
US6617422B1 (en) 1997-05-23 2003-09-09 Peter Nielsen Peptide nucleic acid monomers and oligomers
WO1999034014A2 (en) * 1997-12-23 1999-07-08 Roche Diagnostics Gmbh A method for the determination of a nucleic acid
JP2002517981A (en) * 1998-01-13 2002-06-25 ビオヒプ テヒノロギース ゲゼルシャフト ミット ベシュレンクテル ハフツング Methods for detecting nucleic acid sequences
WO2000034521A1 (en) * 1998-12-08 2000-06-15 Boston Probes, Inc. Methods, kits and compositions for the identification of nucleic acids electrostatically bound to matrices
DK1137807T3 (en) * 1998-12-08 2005-01-24 Boston Probes Inc Methods, Kits and Compositions for Identifying Nucleic Acids Electrostatically Linked to Matrices
EP1192274A2 (en) * 1999-03-25 2002-04-03 Hyseq, Inc. Solution-based methods and materials for sequence analysis by hybridization
US7816098B2 (en) * 2000-01-31 2010-10-19 Sense Proteomic Limited Methods of making and using a protein array
AU2001236589A1 (en) * 2000-02-04 2001-08-14 Aeomica, Inc. Methods and apparatus for high-throughput detection and characterization of alternatively spliced genes
EP1263773A4 (en) 2000-03-14 2005-08-31 Active Motif Oligonucleotide analogues, methods of synthesis and methods of use
US6962906B2 (en) * 2000-03-14 2005-11-08 Active Motif Oligonucleotide analogues, methods of synthesis and methods of use
US20040014644A1 (en) * 2000-03-14 2004-01-22 Vladimir Efimov Oligonucleotide analogues and methods of use for modulating gene expression
GB0406864D0 (en) 2004-03-26 2004-04-28 Qiagen As Nucleic acid sequencing
US20080268451A1 (en) * 2007-03-30 2008-10-30 Bruce Seligmann Measurement of an insoluble analyte in a sample
US20100129902A1 (en) 2008-11-24 2010-05-27 Erhard Ralf Schoenbrunner Replication Stable and RNase Resistant Chimeras of Pestivirus with Insertion in 3' Nontranslated Region (3'NTR)
AU2010315303B2 (en) * 2009-11-03 2015-08-06 Htg Molecular Diagnostics, Inc. Quantitative Nuclease Protection Sequencing (qNPS)
WO2012151111A1 (en) 2011-05-04 2012-11-08 Htg Molecular Diagnostics, Inc. Quantitative nuclease protection assay (qnpa) and sequencing (qnps) improvements
BR112016016130A2 (en) 2014-01-10 2017-08-08 Glaxosmithkline Ip No 2 Ltd COMPOUNDS AND METHODS
US10689689B2 (en) * 2015-12-28 2020-06-23 Roche Molecular Systems, Inc. Generic method for the stabilization of specific RNA

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5578468A (en) * 1987-08-10 1996-11-26 Duke University Site-specific RNA cleavage
DK51092D0 (en) * 1991-05-24 1992-04-15 Ole Buchardt OLIGONUCLEOTIDE ANALOGUE DESCRIBED BY PEN, MONOMERIC SYNTHONES AND PROCEDURES FOR PREPARING THEREOF, AND APPLICATIONS THEREOF
US5539082A (en) * 1993-04-26 1996-07-23 Nielsen; Peter E. Peptide nucleic acids

Also Published As

Publication number Publication date
GB2285445A (en) 1995-07-12
ES2119370T3 (en) 1998-10-01
NO962331D0 (en) 1996-06-05
IL111831A0 (en) 1995-01-24
ATE166066T1 (en) 1998-05-15
FI962340A (en) 1996-06-05
NO962331L (en) 1996-08-06
US5861250A (en) 1999-01-19
JPH09506255A (en) 1997-06-24
AU682563B2 (en) 1997-10-09
KR100221399B1 (en) 1999-10-01
CA2177603C (en) 2000-07-18
DE69410291T2 (en) 1998-10-29
EP0733062A1 (en) 1996-09-25
CA2177603A1 (en) 1995-06-15
IL111831A (en) 2000-12-06
KR960706504A (en) 1996-12-09
ZA949643B (en) 1996-06-05
EP0733062B1 (en) 1998-05-13
FI962340A0 (en) 1996-06-05
DE69410291D1 (en) 1998-06-18
DK0733062T3 (en) 1999-03-15
FI111266B (en) 2003-06-30
AU1272995A (en) 1995-06-27
NO317934B1 (en) 2005-01-10
WO1995015974A1 (en) 1995-06-15

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Legal Events

Date Code Title Description
WAP Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1)