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GB2587178B - Method - Google Patents

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Publication number
GB2587178B
GB2587178B GB1900312.8A GB201900312A GB2587178B GB 2587178 B GB2587178 B GB 2587178B GB 201900312 A GB201900312 A GB 201900312A GB 2587178 B GB2587178 B GB 2587178B
Authority
GB
United Kingdom
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
GB1900312.8A
Other versions
GB201900312D0 (en
GB2587178A (en
Inventor
Jain Nisha
Holme John
Asquith Steven
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
3cr Bioscience Ltd
Original Assignee
3cr Bioscience Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 3cr Bioscience Ltd filed Critical 3cr Bioscience Ltd
Priority to GB1900312.8A priority Critical patent/GB2587178B/en
Publication of GB201900312D0 publication Critical patent/GB201900312D0/en
Priority to PCT/GB2020/050040 priority patent/WO2020144480A1/en
Priority to ES20700852T priority patent/ES2980651T3/en
Priority to EP20700852.5A priority patent/EP3908677B1/en
Priority to US17/309,998 priority patent/US20220090194A1/en
Publication of GB2587178A publication Critical patent/GB2587178A/en
Application granted granted Critical
Publication of GB2587178B publication Critical patent/GB2587178B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/161—A competitive reaction step
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/101—Interaction between at least two labels
    • C12Q2565/1015—Interaction between at least two labels labels being on the same oligonucleotide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/107—Alteration in the property of hybridised versus free label oligonucleotides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
GB1900312.8A 2019-01-09 2019-01-09 Method Active GB2587178B (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
GB1900312.8A GB2587178B (en) 2019-01-09 2019-01-09 Method
PCT/GB2020/050040 WO2020144480A1 (en) 2019-01-09 2020-01-09 Method for detecting a nucleic acid sequence
ES20700852T ES2980651T3 (en) 2019-01-09 2020-01-09 Method for detecting a nucleic acid sequence
EP20700852.5A EP3908677B1 (en) 2019-01-09 2020-01-09 Method for detecting a nucleic acid sequence
US17/309,998 US20220090194A1 (en) 2019-01-09 2020-01-09 Method for detecting a nucleic acid sequence

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB1900312.8A GB2587178B (en) 2019-01-09 2019-01-09 Method

Publications (3)

Publication Number Publication Date
GB201900312D0 GB201900312D0 (en) 2019-02-27
GB2587178A GB2587178A (en) 2021-03-24
GB2587178B true GB2587178B (en) 2021-10-06

Family

ID=65528023

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1900312.8A Active GB2587178B (en) 2019-01-09 2019-01-09 Method

Country Status (1)

Country Link
GB (1) GB2587178B (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6270967B1 (en) * 1996-05-04 2001-08-07 Zeneca Limited Method for detecting a nucleic acid base sequence
US20060141518A1 (en) * 2004-03-24 2006-06-29 Lao Kai Q Detection of gene expression
GB2526445A (en) * 2014-07-14 2015-11-25 Identigen Ltd A method for detecting PCR amplification in a sample
WO2016172632A2 (en) * 2015-04-24 2016-10-27 Atila Biosystems, Inc Amplification with primers of limited nucleotide composition

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0510979D0 (en) 2005-05-28 2005-07-06 Kbiosciences Ltd Detection system for PCR assay

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6270967B1 (en) * 1996-05-04 2001-08-07 Zeneca Limited Method for detecting a nucleic acid base sequence
US20060141518A1 (en) * 2004-03-24 2006-06-29 Lao Kai Q Detection of gene expression
GB2526445A (en) * 2014-07-14 2015-11-25 Identigen Ltd A method for detecting PCR amplification in a sample
WO2016172632A2 (en) * 2015-04-24 2016-10-27 Atila Biosystems, Inc Amplification with primers of limited nucleotide composition

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Applied and Environmental Microbiology, Vol. 71, 2005, Zhili H.E. et al., "Empirical establishment of oligonucleotide probe design criteria " pp. 3753 - 3760 *
Clinical Chemistry, Vol. 59, 2013, Faltin B. et al., "Current Methods for Fluorescence-Based Universal Sequence-Dependent Detection of Nucleic Acids in Homogenous Assays and Clinical Applications", pp. 1567-1582 *
PCR Methods & Applications, Vol. 6, 1996, "Uniform Amplification of a mixture of deoxyribonucleic acids with varying GC content", pp. 633-638 *

Also Published As

Publication number Publication date
GB201900312D0 (en) 2019-02-27
GB2587178A (en) 2021-03-24

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