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FR3055339B1 - METHOD FOR IN VITRO DETECTION AND IDENTIFICATION OF ONE OR MORE TARGET PATHOGENS PRESENT IN A BIOLOGICAL SAMPLE - Google Patents

METHOD FOR IN VITRO DETECTION AND IDENTIFICATION OF ONE OR MORE TARGET PATHOGENS PRESENT IN A BIOLOGICAL SAMPLE Download PDF

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Publication number
FR3055339B1
FR3055339B1 FR1658143A FR1658143A FR3055339B1 FR 3055339 B1 FR3055339 B1 FR 3055339B1 FR 1658143 A FR1658143 A FR 1658143A FR 1658143 A FR1658143 A FR 1658143A FR 3055339 B1 FR3055339 B1 FR 3055339B1
Authority
FR
France
Prior art keywords
identification
biological sample
vitro detection
target pathogens
pathogens present
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
FR1658143A
Other languages
French (fr)
Other versions
FR3055339A1 (en
Inventor
Cyrielle Gasc
Jeremie Denonfoux
Eric Peyretaillade
Pierre Peyret
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Universite Blaise Pascal Clermont Ferrand II
Universite Clermont Auvergne
Original Assignee
Centre National de la Recherche Scientifique CNRS
Universite Blaise Pascal Clermont Ferrand II
Universite Clermont Auvergne
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS, Universite Blaise Pascal Clermont Ferrand II, Universite Clermont Auvergne filed Critical Centre National de la Recherche Scientifique CNRS
Priority to FR1658143A priority Critical patent/FR3055339B1/en
Priority to PCT/EP2017/071488 priority patent/WO2018041747A1/en
Publication of FR3055339A1 publication Critical patent/FR3055339A1/en
Application granted granted Critical
Publication of FR3055339B1 publication Critical patent/FR3055339B1/en
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La présente invention concerne une méthode de détection et d'identification in vitro d'un ou plusieurs pathogènes cibles présents dans un échantillon biologique, mettant en œuvre une méthode de capture de gènes couplée à du séquençage haut-débit, la méthode de capture de gènes permettant la capture de fragments d'ADN ayant une longueur d'au moins 6 000 paires de bases.The present invention relates to a method of in vitro detection and identification of one or more target pathogens present in a biological sample, implementing a gene capture method coupled with high-throughput sequencing, the gene capture method allowing the capture of DNA fragments having a length of at least 6000 base pairs.

FR1658143A 2016-09-01 2016-09-01 METHOD FOR IN VITRO DETECTION AND IDENTIFICATION OF ONE OR MORE TARGET PATHOGENS PRESENT IN A BIOLOGICAL SAMPLE Active FR3055339B1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
FR1658143A FR3055339B1 (en) 2016-09-01 2016-09-01 METHOD FOR IN VITRO DETECTION AND IDENTIFICATION OF ONE OR MORE TARGET PATHOGENS PRESENT IN A BIOLOGICAL SAMPLE
PCT/EP2017/071488 WO2018041747A1 (en) 2016-09-01 2017-08-28 Method for the in vitro detection and identification of at least one target pathogen present in a biological sample

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1658143 2016-09-01
FR1658143A FR3055339B1 (en) 2016-09-01 2016-09-01 METHOD FOR IN VITRO DETECTION AND IDENTIFICATION OF ONE OR MORE TARGET PATHOGENS PRESENT IN A BIOLOGICAL SAMPLE

Publications (2)

Publication Number Publication Date
FR3055339A1 FR3055339A1 (en) 2018-03-02
FR3055339B1 true FR3055339B1 (en) 2020-05-22

Family

ID=57539388

Family Applications (1)

Application Number Title Priority Date Filing Date
FR1658143A Active FR3055339B1 (en) 2016-09-01 2016-09-01 METHOD FOR IN VITRO DETECTION AND IDENTIFICATION OF ONE OR MORE TARGET PATHOGENS PRESENT IN A BIOLOGICAL SAMPLE

Country Status (2)

Country Link
FR (1) FR3055339B1 (en)
WO (1) WO2018041747A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110684765A (en) * 2019-09-20 2020-01-14 浙江大学 Screening method of dementia-related pathogenic gene capture probe, captured genome, product and application
CN113512600B (en) * 2021-06-18 2023-04-11 广西壮族自治区水牛研究所 Primer and method for detecting pseudomonas putida and application thereof
CN117987578B (en) * 2024-04-03 2024-07-09 北京市动物疫病预防控制中心 Microfluidic chip for simultaneously detecting pathogenic escherichia coli, salmonella and drug resistance genes and application thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10227635B2 (en) * 2012-04-16 2019-03-12 Molecular Loop Biosolutions, Llc Capture reactions
US20140274741A1 (en) * 2013-03-15 2014-09-18 The Translational Genomics Research Institute Methods to capture and sequence large fragments of dna and diagnostic methods for neuromuscular disease
US9896686B2 (en) * 2014-01-09 2018-02-20 AgBiome, Inc. High throughput discovery of new genes from complex mixtures of environmental microbes

Also Published As

Publication number Publication date
FR3055339A1 (en) 2018-03-02
WO2018041747A1 (en) 2018-03-08

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Owner name: UNIVERSITE CLERMONT AUVERGNE, FR

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Owner name: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, FR

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