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FR2602241A1 - Plate for the microscopic examination of living cell cultures - Google Patents

Plate for the microscopic examination of living cell cultures Download PDF

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Publication number
FR2602241A1
FR2602241A1 FR8611024A FR8611024A FR2602241A1 FR 2602241 A1 FR2602241 A1 FR 2602241A1 FR 8611024 A FR8611024 A FR 8611024A FR 8611024 A FR8611024 A FR 8611024A FR 2602241 A1 FR2602241 A1 FR 2602241A1
Authority
FR
France
Prior art keywords
microscope
well
glass
cover
cell cultures
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
FR8611024A
Other languages
French (fr)
Inventor
Pierre Travo
Philippe Bodin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jouan SA
Original Assignee
Jouan SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jouan SA filed Critical Jouan SA
Priority to FR8611024A priority Critical patent/FR2602241A1/en
Publication of FR2602241A1 publication Critical patent/FR2602241A1/en
Withdrawn legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/20—Material Coatings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/045—Connecting closures to device or container whereby the whole cover is slidable

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Clinical Laboratory Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Sustainable Development (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Microscoopes, Condenser (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to a plate for the microscopic examination of living cell cultures. This plate comprises at least one well 12 whose bottom is made of a glass microscope slide 14 and whose walls are made from a food-grade plastic material, the said wells being assembled with the slide by gluing, the height of the assembly being chosen to allow the passage of the plate between the stage and the front lens of a microscope condenser.

Description

Botte Pour í lobservation au microscope de cultures cellulaires vivantes. Boot For microscopic observation of living cell cultures.

Dans l'état actuel de la technique, il est pratiquement impossible d'observer des cultures cellulaires vivantes à l'aide d'un microscope utilisant une optique à lumière polarisée, par exemple le contraste d'interférence différentieN
Les boîtes de Petri qui sont couramment utilisées pour contenir les cultures cellulaires ne se prêtent pas à une telle observation, étant donné que la matière plastique dans laquelle elles sont réalisées est fortement dépolarisante.In the current state of the art, it is practically impossible to observe living cell cultures using a microscope using polarized light optics, for example differentiated interference contrast.
The Petri dishes which are commonly used to contain cell cultures do not lend themselves to such an observation, since the plastic material in which they are made is highly depolarizing.

De plus, leur grande hauteur, même pour les plus petits modèles, en interdit le passage entre la platine du microscope et la lentille frontale du condenseur.In addition, their great height, even for the smallest models, prevents them from passing between the stage of the microscope and the front lens of the condenser.

La présente invention vise à remédier à ces inconvénients et à cet effet, elle concerne une bote pour l'observation au microscope de cultures cellulaires, qui se caractérise en ce qu'elle comprend au moins un puits dont le fond est réalisé par une lame porte-objet en verre pour microscope, et dont les parois sont réalisées en une matière plastique de qualité alimentaire, ledit puitsétant assemblé à la lame porte-objet par collage, la hauteur de l'ensemble étant choisie pour permettre le passage de la boîte entre la platine et la lentille frontale d'un condenseur de microscope. The present invention aims to remedy these drawbacks and to this end, it relates to a box for the observation under the microscope of cell cultures, which is characterized in that it comprises at least one well, the bottom of which is produced by a holder blade. - glass object for microscope, and whose walls are made of a food-grade plastic, said well being assembled to the object-slide by gluing, the height of the assembly being chosen to allow the passage of the box between the stage and the front lens of a microscope condenser.

La boIte est recouverte d'un couvercle constitué d'une fine lamelle de verre. Celle-ci est maintenue en place par des moyens de guidage, par exemple par des glissières latérales formées sur le bord de la face supérieure de la boite. The box is covered with a cover made of a thin glass strip. The latter is held in place by guiding means, for example by lateral slides formed on the edge of the upper face of the box.

Ces boltes sont entreposées dans un incubateur à gaz carbonique et les échanges gazeux avec I'atmosphère de l'incubateur s'effectuent à travers les minusculs passages formés entre le couvercle et les rugosités de surface du corps.These bolts are stored in a carbon dioxide incubator and the gas exchanges with the atmosphere of the incubator take place through the tiny passages formed between the cover and the surface roughness of the body.

Un mode de réalisation de l'invention sera décrit à présent en détail à titre d'exemple non limitatif, en regard de l'unique figure annexée qui représente une vue en perspective de la boîte et de son couvercle.  An embodiment of the invention will now be described in detail by way of nonlimiting example, with reference to the single appended figure which represents a perspective view of the box and its cover.

La botte comprend un corps 10 réalisé en une matière plastique alimentaire, par exemple en polychlorure de vinyle, et dans l'exemple illustré, elle a la forme d'un parallélépi perde rectangle aplati pourvu de trois alvéoles ou puits 12 disposés cate-à-cate sur la longueur du corps et qui traversent ce dernier de part en part. Mais, une telle forme n'est pas limitative, car le corps peut être également en forme de disque circulaire ou ovale ou avoir toute autre forme aplatie De même, il peut comporter un nombre quelconaue de puits disposés en une ou plusieurs rangées, ou radialement0 Les puits sont séparés par des cloisons 13. The boot comprises a body 10 made of a food-grade plastic material, for example polyvinyl chloride, and in the example illustrated, it has the shape of a flat rectangular rectangle parallelepi provided with three cells or wells 12 arranged cate-à- cate along the length of the body and which cross the latter right through. However, such a shape is not limiting, since the body can also be in the form of a circular or oval disc or have any other flattened shape. Similarly, it can include any number of wells arranged in one or more rows, or radially. The wells are separated by partitions 13.

Sur l'une des grandes faces du corps 10 est fixée par tout moyen approprié, et de préférence par collage, une lame porte-objet 14 en verre pour microscope, destinée à constituer le fond des puits et à assurer en même tenps la rigidité du corps, Sur cette lame et dans chaque puits, est déposée une certaine quantité de suspension de culture cellulaire. On one of the large faces of the body 10 is fixed by any appropriate means, and preferably by gluing, a glass slide 14 made of glass for microscope, intended to constitute the bottom of the wells and at the same time ensure the rigidity of the body, On this slide and in each well, a certain amount of cell culture suspension is deposited.

Sur son autre grande face, le corps présente deux rebords longitudinaux 16, 18 surélevés par rapport au plan de ladite face et longeant les deux catés longitudinaux du corps, et un rebord transversal 20. Un couvercle 22 de surface sensiblement égale à celle définie entre les trois rebords et constitué par une fine lame de verre, peut être glissé entre les rebords longitudinaux jusqu a venir en butée contre le rebord transversal. On its other large face, the body has two longitudinal edges 16, 18 raised relative to the plane of said face and along the two longitudinal sides of the body, and a transverse edge 20. A cover 22 of surface area substantially equal to that defined between the three edges and constituted by a thin glass blade, can be slid between the longitudinal edges until it comes into abutment against the transverse edge.

Les cultures cellulaires vivantes contenues dans les puits doivent être en communication avec le milieu gazeux extérieur. I1 n'est toutefois pas nécessaire pour cela de former sur le corps des ouvertures de communication. En effet, les rugosités de surface de la matière plastique suffisent à établir de points d'échange gazeux avec l'atmosphare de l'incubateur. The living cell cultures contained in the wells must be in communication with the external gaseous medium. I1 is however not necessary for this to form on the body of the communication openings. In fact, the surface roughness of the plastic material is sufficient to establish gas exchange points with the atmosphere of the incubator.

Comme on l'a déjà précisé, la hauteur de la boite est choisie pour que cette dernière puisse être passée entre la platine d'un microscope et la lentille frontale du condenseur. De plus, le rapport surface/hauteur de la boite est choisi pour faciliter les échanges gazeux. As already specified, the height of the box is chosen so that it can be passed between the stage of a microscope and the front lens of the condenser. In addition, the surface / height ratio of the box is chosen to facilitate gas exchange.

Après la fabrication et l'assemblage de la boite, celle-ci est soumise, avant la premiere utilisation, à une série de traitements, tels que des rinçages à l'eau, afin d'éliminer le maximum de substances toxiques diffusibles, contenues dans la matière plastique et dans la colle, puis à une stérilisation en la rinçant par exemple avec de l'alcool éthylique. After the manufacture and assembly of the box, it is subjected, before the first use, to a series of treatments, such as rinsing with water, in order to remove the maximum of diffusible toxic substances, contained in the plastic and in the glue, then to sterilization by rinsing it for example with ethyl alcohol.

En utilisation chaque puits peut recevoir jusqu'à 0,4 ml de suspension cellulaire.  In use each well can receive up to 0.4 ml of cell suspension.

Claims (6)

REVENDICATIONS 1.- Boute pour l'observation au microscope de cultures cellulaires vivantes, caractérisée en ce qu'elle comprend au moins un puits (12) dont le fond est réalisé par une lame porte-objet (14) en verre pour microscope, et dont les parois sont réalisées en une matière plastique de qualité alimentaire, ledit puits étant assemblé à la lame porte-objet par collage, la hauteur de l'ensemble étant choisie pour permettre le passage de la botte entre la platine et la lentille frontale d'un condenseur de microscope. 1.- Boute for the observation under the microscope of living cell cultures, characterized in that it comprises at least one well (12), the bottom of which is produced by a glass slide (14) made of glass for microscope, and of which the walls are made of a food-grade plastic, said well being assembled to the microscope slide by gluing, the height of the assembly being chosen to allow passage of the boot between the plate and the front lens of a microscope condenser. 2.- Botte selon la revendication 1, caractériséeen ce qu'elle est recouverte d'un couvercle (22) constitué d'une fine lamelle de verre. 2.- Boot according to claim 1, characterized in that it is covered with a cover (22) consisting of a thin glass strip. 3.- Botte selon la revendication 1, caractérisée en ce qu'elle comprend un corps plat en matiere plastique alimentaire, des forme quelconque et de préférence en forme de parallélépipède ou de disque circulaire ou ovale, dans lequel sont formés un ou plusieurs alvéoles distincts ou puits (12) qui le traversent de part en part. 3.- Boot according to claim 1, characterized in that it comprises a flat body of food plastic, any shape and preferably in the form of a parallelepiped or circular or oval disc, in which are formed one or more distinct alveoli or well (12) which pass right through it. 4.- Botte selon l'une des revendications précédentes, caractérisée en ce que la lame porte-objet (14) est collée sur l'une des grandes faces dudit corps et en ce que le corps est pourvu sur son autre grande face de moyens de guidage et de maintien permettant de mettre en place et de maintenir la lamelle en verre. 4.- Boot according to one of the preceding claims, characterized in that the object-holder blade (14) is glued to one of the large faces of said body and in that the body is provided on its other large face with means guiding and holding for positioning and maintaining the glass coverslip. 5.- Boîte selon la revendication 4, caractérisée en ce que lesdits moyens de guidage et de maintien sont constitués par deux rebords parallèles (16, 18) disposés par exemple le long des deux côtés longitudinaux du corps, et surélevés par rapport au plan de ladite face. 5.- Box according to claim 4, characterized in that said guide and holding means are constituted by two parallel flanges (16, 18) arranged for example along the two longitudinal sides of the body, and raised relative to the plane of said face. 6.- Botte selon la revendication 5, caractérisée en ce qu'un troisième rebord surélevé (20) est également formé le long d'un côté transversal du corps de manière a constituer une butée pour le couvercle dans l'un des sens de glissement, ledit couvercle ayant une surface sensiblement égale à celle définie entre les rebords longitudinaux et le rebord transversal.  6.- Boot according to claim 5, characterized in that a third raised rim (20) is also formed along a transverse side of the body so as to constitute a stop for the cover in one of the sliding directions , said cover having an area substantially equal to that defined between the longitudinal edges and the transverse edge.
FR8611024A 1986-07-30 1986-07-30 Plate for the microscopic examination of living cell cultures Withdrawn FR2602241A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR8611024A FR2602241A1 (en) 1986-07-30 1986-07-30 Plate for the microscopic examination of living cell cultures

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR8611024A FR2602241A1 (en) 1986-07-30 1986-07-30 Plate for the microscopic examination of living cell cultures

Publications (1)

Publication Number Publication Date
FR2602241A1 true FR2602241A1 (en) 1988-02-05

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FR8611024A Withdrawn FR2602241A1 (en) 1986-07-30 1986-07-30 Plate for the microscopic examination of living cell cultures

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FR (1) FR2602241A1 (en)

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993007254A1 (en) * 1991-10-04 1993-04-15 Istituto Di Ricerche Biologiche F. Redi Microculture slide ready for use
WO2002027380A1 (en) * 2000-09-28 2002-04-04 Radiumhospitalets Forskningsstiftelse Microscope slide
WO2002042824A2 (en) * 2000-11-24 2002-05-30 P.A.L.M. Microlaser Technologies Ag Receptacle for receiving an object, in particular a biological object, to be examined by a microscope
GR20060100290A (en) * 2006-05-15 2008-01-16 Γ.Παπανικολαου-Σ.Σοφου Ο.Ε. Microsope dish with a fixed slide and a sealing cover as well as device for fixing a rack of an array of microbiological-testing tubes.
EP1886792A3 (en) * 2004-03-22 2008-05-14 ibidi GmbH Device for microscope investigation
EP2681757A2 (en) * 2011-03-03 2014-01-08 California Institute of Technology E-petri dishes, devices, and systems
US9343494B2 (en) 2011-03-03 2016-05-17 California Institute Of Technology Light guided pixel configured for emissions detection and comprising a guide layer with a wavelength selective filter material and a light detector layer
US9426429B2 (en) 2010-10-26 2016-08-23 California Institute Of Technology Scanning projective lensless microscope system
US9569664B2 (en) 2010-10-26 2017-02-14 California Institute Of Technology Methods for rapid distinction between debris and growing cells
US9643184B2 (en) 2010-10-26 2017-05-09 California Institute Of Technology e-Petri dishes, devices, and systems having a light detector for sampling a sequence of sub-pixel shifted projection images
US9743020B2 (en) 2010-03-23 2017-08-22 California Institute Of Technology Super resolution optofluidic microscopes for 2D and 3D imaging

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3620596A (en) * 1970-09-18 1971-11-16 Aerojet General Co Microscope slides
US3656833A (en) * 1970-06-29 1972-04-18 Medical Plastics Inc Combined plastic-glass microscope slides
FR2114673A5 (en) * 1970-11-18 1972-06-30 Miles Lab
US3928142A (en) * 1973-07-24 1975-12-23 Dennis B Smith Culture chamber for the study of biological systems and method of fabrication thereof
US4171866A (en) * 1978-04-20 1979-10-23 Tolles Walter E Disposable volumetric slide
US4501496A (en) * 1982-05-07 1985-02-26 Griffin Gladys B Specimen slide for analysis of liquid specimens

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3656833A (en) * 1970-06-29 1972-04-18 Medical Plastics Inc Combined plastic-glass microscope slides
US3620596A (en) * 1970-09-18 1971-11-16 Aerojet General Co Microscope slides
FR2114673A5 (en) * 1970-11-18 1972-06-30 Miles Lab
US3928142A (en) * 1973-07-24 1975-12-23 Dennis B Smith Culture chamber for the study of biological systems and method of fabrication thereof
US4171866A (en) * 1978-04-20 1979-10-23 Tolles Walter E Disposable volumetric slide
US4501496A (en) * 1982-05-07 1985-02-26 Griffin Gladys B Specimen slide for analysis of liquid specimens

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993007254A1 (en) * 1991-10-04 1993-04-15 Istituto Di Ricerche Biologiche F. Redi Microculture slide ready for use
WO2002027380A1 (en) * 2000-09-28 2002-04-04 Radiumhospitalets Forskningsstiftelse Microscope slide
WO2002042824A2 (en) * 2000-11-24 2002-05-30 P.A.L.M. Microlaser Technologies Ag Receptacle for receiving an object, in particular a biological object, to be examined by a microscope
WO2002042824A3 (en) * 2000-11-24 2002-08-08 Palm Microlaser Tech Gmbh Receptacle for receiving an object, in particular a biological object, to be examined by a microscope
EP1886792A3 (en) * 2004-03-22 2008-05-14 ibidi GmbH Device for microscope investigation
GR20060100290A (en) * 2006-05-15 2008-01-16 Γ.Παπανικολαου-Σ.Σοφου Ο.Ε. Microsope dish with a fixed slide and a sealing cover as well as device for fixing a rack of an array of microbiological-testing tubes.
US9743020B2 (en) 2010-03-23 2017-08-22 California Institute Of Technology Super resolution optofluidic microscopes for 2D and 3D imaging
US9426429B2 (en) 2010-10-26 2016-08-23 California Institute Of Technology Scanning projective lensless microscope system
US9569664B2 (en) 2010-10-26 2017-02-14 California Institute Of Technology Methods for rapid distinction between debris and growing cells
US9643184B2 (en) 2010-10-26 2017-05-09 California Institute Of Technology e-Petri dishes, devices, and systems having a light detector for sampling a sequence of sub-pixel shifted projection images
US9343494B2 (en) 2011-03-03 2016-05-17 California Institute Of Technology Light guided pixel configured for emissions detection and comprising a guide layer with a wavelength selective filter material and a light detector layer
EP2681757A4 (en) * 2011-03-03 2014-07-23 California Inst Of Techn E-petri dishes, devices, and systems
EP2681757A2 (en) * 2011-03-03 2014-01-08 California Institute of Technology E-petri dishes, devices, and systems

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