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ES2075155T3 - ENZYMATIC SEPARATION OF A SEQUENCE OF A PROTEIN WITH TERMINAL AMINO. - Google Patents

ENZYMATIC SEPARATION OF A SEQUENCE OF A PROTEIN WITH TERMINAL AMINO.

Info

Publication number
ES2075155T3
ES2075155T3 ES90304890T ES90304890T ES2075155T3 ES 2075155 T3 ES2075155 T3 ES 2075155T3 ES 90304890 T ES90304890 T ES 90304890T ES 90304890 T ES90304890 T ES 90304890T ES 2075155 T3 ES2075155 T3 ES 2075155T3
Authority
ES
Spain
Prior art keywords
protein
sequence
terminal amino
compounds
enzymatic separation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90304890T
Other languages
Spanish (es)
Inventor
Gerald Wayne Becker
Thomas Charles Furman
Warren C Mackellar
James Patrick Mcdonough
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eli Lilly and Co
Original Assignee
Eli Lilly and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eli Lilly and Co filed Critical Eli Lilly and Co
Application granted granted Critical
Publication of ES2075155T3 publication Critical patent/ES2075155T3/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Endocrinology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Diabetes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

This disclosure relates to new compounds and to a process for the controlled conversion of such compounds to produce a Protein that comprises an amino acid sequence which is useful as a precursor to human insulin or to a modified human insulin, the amino terminus portion of which sequence does not define a cathepsin C dipeptide removal stop point. The compounds have the formula Met-Y-Protein in which Y is Tyr or Arg.
ES90304890T 1989-05-09 1990-05-04 ENZYMATIC SEPARATION OF A SEQUENCE OF A PROTEIN WITH TERMINAL AMINO. Expired - Lifetime ES2075155T3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/349,472 US5126249A (en) 1989-05-09 1989-05-09 Enzymatic removal of a protein amino-terminal sequence

Publications (1)

Publication Number Publication Date
ES2075155T3 true ES2075155T3 (en) 1995-10-01

Family

ID=23372543

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90304890T Expired - Lifetime ES2075155T3 (en) 1989-05-09 1990-05-04 ENZYMATIC SEPARATION OF A SEQUENCE OF A PROTEIN WITH TERMINAL AMINO.

Country Status (12)

Country Link
US (2) US5126249A (en)
EP (1) EP0397420B1 (en)
JP (1) JP2592981B2 (en)
AT (1) ATE124997T1 (en)
AU (1) AU630951B2 (en)
CA (1) CA2016049A1 (en)
DE (1) DE69020794T2 (en)
DK (1) DK0397420T3 (en)
ES (1) ES2075155T3 (en)
HU (1) HU215582B (en)
IE (1) IE67531B1 (en)
IL (1) IL94305A (en)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ232375A (en) * 1989-02-09 1992-04-28 Lilly Co Eli Insulin analogues modified at b29
US5514646A (en) * 1989-02-09 1996-05-07 Chance; Ronald E. Insulin analogs modified at position 29 of the B chain
IL104086A (en) * 1991-12-18 1998-03-10 Lilly Co Eli Translational activating oligonucleotide sequence useful for the high level expression of polypeptides and a method for producing such polypeptides
CA2089541A1 (en) * 1992-02-18 1993-08-19 Bruce H. Frank Selective removal of n-terminal extensions from folded insulin precursors using dipeptidyl-aminopeptidase-1
TW257792B (en) * 1992-10-01 1995-09-21 Lilly Co Eli
JP2887060B2 (en) * 1993-06-30 1999-04-26 日清製粉株式会社 Gricentin production method
US5573923A (en) * 1993-12-22 1996-11-12 Eli Lilly And Company Method for removing N-terminal dipeptides from precursor polypeptides with immobilized dipeptidylaminopeptidase from dictyostelium discoideum
JP2887063B2 (en) * 1993-12-28 1999-04-26 日清製粉株式会社 Gricentin manufacturing method
DK0759931T3 (en) * 1994-05-09 1999-07-12 Unizyme Lab Aps Enzymatic process for producing a desired protein from an amino terminal extended protein
CZ290079B6 (en) * 1994-12-29 2002-05-15 Bio-Technology General Corp. Process for preparing insulin and intermediate for this process
US5840517A (en) * 1995-04-26 1998-11-24 Eli Lilly And Company Process for preparing obesity protein analogs
US5614379A (en) * 1995-04-26 1997-03-25 Eli Lilly And Company Process for preparing anti-obesity protein
US6008041A (en) * 1996-05-08 1999-12-28 Eli Lilly And Company Bovine dipeptidylaminopeptidase 1
EP0938504A2 (en) * 1996-06-07 1999-09-01 Eli Lilly And Company Ob PROTEIN BINDING PROTEIN
DE19943177C2 (en) * 1999-09-09 2002-10-24 Dieter Jenne Process for the production of active series proteases and inactive variants
IL141647A0 (en) * 2001-02-26 2002-03-10 Yeda Res & Dev Synthetic human peptides and pharmaceutical compositions comprising them for the treatment of systemic lupus erythematosus
ES2295370T3 (en) * 2001-07-06 2008-04-16 Lg Life Sciences Ltd. NEW AMINOPEPTIDASA DERIVED FROM BACILLUS LICHENIFORMIS, GEN CODIFYING THE AMINOPEPTIDASE, VECTOR OF EXPRESSION CONTAINING THE GEN, TRANSFORMANT AND METHOD FOR PREPARATION.
ATE512674T1 (en) * 2003-01-06 2011-07-15 Angiochem Inc ANGIOPEP-1, RELATED COMPOUNDS, AND USES THEREOF
NZ541658A (en) * 2003-01-14 2008-04-30 Teva Pharma Parenteral formulations of peptides for the treatment of systemic lupus erythematosus
MXPA05007552A (en) * 2003-01-14 2006-05-19 Teva Pharma Parenteral formulations of a peptide for the treatment of systemic lupus erythematosus.
US8618054B2 (en) * 2004-05-05 2013-12-31 Valorisation-Rechereche Société en Commandite Interleukin-1 receptor antagonists, compositions, and methods of treatment
CA2607113C (en) 2004-05-05 2013-11-12 Valorisation-Recherche, Societe En Commandite Interleukin-1 receptor antagonists, compositions, and methods of treatment
US20090048161A1 (en) 2005-05-05 2009-02-19 Valorisation Hsj, Societe En Commandite Cytokine receptor modulators and uses thereof
US9365634B2 (en) * 2007-05-29 2016-06-14 Angiochem Inc. Aprotinin-like polypeptides for delivering agents conjugated thereto to tissues
EP2789628A3 (en) 2007-05-29 2015-03-04 Angiochem Inc. Aprotinin-like polypeptides for delivering agents conjugated thereto to tissues
ES2721148T3 (en) 2008-04-18 2019-07-29 Angiochem Inc Pharmaceutical compositions of paclitaxel, paclitaxel analogues or paclitaxel conjugates and related methods of preparation and use
WO2010043049A1 (en) 2008-10-15 2010-04-22 Angiochem Inc. Etoposide and doxorubicin conjugates for drug delivery
EP2346906A4 (en) 2008-10-15 2013-04-24 Angiochem Inc Conjugates of glp-1 agonists and uses thereof
AU2009322043A1 (en) 2008-12-05 2011-07-07 Angiochem Inc. Conjugates of neurotensin or neurotensin analogs and uses thereof
US8853353B2 (en) 2008-12-17 2014-10-07 Angiochem, Inc. Membrane type-1 matrix metalloprotein inhibitors and uses thereof
MX2011011023A (en) 2009-04-20 2012-01-20 Angiochem Inc Treatment of ovarian cancer using an anticancer agent conjugated to an angiopep-2 analog.
CA2766537A1 (en) 2009-07-02 2011-01-06 Angiochem Inc. Multimeric peptide conjugates and uses thereof
JP2013530993A (en) 2010-07-02 2013-08-01 アンジオケム インコーポレーテッド Short and D-amino acid containing polypeptides for therapeutic conjugates and uses thereof
WO2014096985A2 (en) * 2012-12-19 2014-06-26 Wockhardt Limited A stable aqueous composition comprising human insulin or an analogue or derivative thereof
US11326255B2 (en) 2013-02-07 2022-05-10 Uchicago Argonne, Llc ALD reactor for coating porous substrates
WO2016205367A1 (en) 2015-06-15 2016-12-22 Angiochem Inc. Methods for the treatment of leptomeningeal carcinomatosis
US11111578B1 (en) 2020-02-13 2021-09-07 Uchicago Argonne, Llc Atomic layer deposition of fluoride thin films
US12065738B2 (en) 2021-10-22 2024-08-20 Uchicago Argonne, Llc Method of making thin films of sodium fluorides and their derivatives by ALD
US11901169B2 (en) 2022-02-14 2024-02-13 Uchicago Argonne, Llc Barrier coatings

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ199391A (en) * 1981-01-02 1985-12-13 Genentech Inc Chimeric polypeptides comprising a proinsulin sequence,and preparation by recombinant dna technique;production of human insulin
DK55685A (en) * 1985-02-07 1986-08-08 Nordisk Gentofte ENZYM OR ENZYM COMPLEX WITH PROTEOLYTIC ACTIVITY
DE3586773T2 (en) * 1984-08-16 1993-03-18 Bio Technology General Corp METHOD FOR THE REMOVAL OF N-ENDAMINOAID REMAINS FROM EUKARYOTPOLYPEPTIDE PLANTS AND POLYPEPTIDES GENERATED IN THIS PROCESS.
US4946828A (en) * 1985-03-12 1990-08-07 Novo Nordisk A/S Novel insulin peptides
US4865974A (en) * 1985-09-20 1989-09-12 Cetus Corporation Bacterial methionine N-terminal peptidase
PT83613B (en) * 1985-10-28 1988-11-21 Lilly Co Eli Process for the selective chemical removal of a protein amino-terminal residue
IL91771A0 (en) * 1988-09-30 1990-06-10 Lilly Co Eli Increased expression of small molecular weight recombinant proteins
AU641631B2 (en) * 1988-12-23 1993-09-30 Novo Nordisk A/S Human insulin analogues
NZ232375A (en) * 1989-02-09 1992-04-28 Lilly Co Eli Insulin analogues modified at b29

Also Published As

Publication number Publication date
AU5483390A (en) 1990-11-15
CA2016049A1 (en) 1990-11-09
IL94305A (en) 1995-12-08
HU902697D0 (en) 1990-09-28
EP0397420B1 (en) 1995-07-12
IE67531B1 (en) 1996-04-03
AU630951B2 (en) 1992-11-12
DK0397420T3 (en) 1995-08-21
JP2592981B2 (en) 1997-03-19
IL94305A0 (en) 1991-03-10
HUT54213A (en) 1991-01-28
US5126249A (en) 1992-06-30
DE69020794D1 (en) 1995-08-17
IE901673L (en) 1990-11-09
HU215582B (en) 1999-01-28
DE69020794T2 (en) 1995-12-14
JPH0334995A (en) 1991-02-14
EP0397420A1 (en) 1990-11-14
ATE124997T1 (en) 1995-07-15
US5352769A (en) 1994-10-04

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