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EP3810805A4 - Method for detection and quantification of genetic alterations - Google Patents

Method for detection and quantification of genetic alterations Download PDF

Info

Publication number
EP3810805A4
EP3810805A4 EP19825310.6A EP19825310A EP3810805A4 EP 3810805 A4 EP3810805 A4 EP 3810805A4 EP 19825310 A EP19825310 A EP 19825310A EP 3810805 A4 EP3810805 A4 EP 3810805A4
Authority
EP
European Patent Office
Prior art keywords
quantification
detection
genetic alterations
genetic
alterations
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP19825310.6A
Other languages
German (de)
French (fr)
Other versions
EP3810805A1 (en
Inventor
Yukti CHOUDHURY
Hao Chen
Min-Han Tan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lucence Life Sciences Pte Ltd
Original Assignee
Lucence Life Sciences Pte Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lucence Life Sciences Pte Ltd filed Critical Lucence Life Sciences Pte Ltd
Publication of EP3810805A1 publication Critical patent/EP3810805A1/en
Publication of EP3810805A4 publication Critical patent/EP3810805A4/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP19825310.6A 2018-06-25 2019-06-25 Method for detection and quantification of genetic alterations Pending EP3810805A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SG10201805450Y 2018-06-25
PCT/SG2019/050317 WO2020005159A1 (en) 2018-06-25 2019-06-25 Method for detection and quantification of genetic alterations

Publications (2)

Publication Number Publication Date
EP3810805A1 EP3810805A1 (en) 2021-04-28
EP3810805A4 true EP3810805A4 (en) 2022-03-23

Family

ID=68985923

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19825310.6A Pending EP3810805A4 (en) 2018-06-25 2019-06-25 Method for detection and quantification of genetic alterations

Country Status (4)

Country Link
EP (1) EP3810805A4 (en)
CN (1) CN112639127A (en)
SG (1) SG11202012687SA (en)
WO (1) WO2020005159A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113436679B (en) * 2020-03-23 2024-05-10 北京合生基因科技有限公司 Method and system for determining mutation rate of nucleic acid sample to be tested
WO2022025823A1 (en) * 2020-07-29 2022-02-03 Lucence Life Sciences Pte. Ltd Methods and kits for detecting and sequencing rna viruses

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011019964A1 (en) * 2009-08-12 2011-02-17 Nugen Technologies, Inc. Methods, compositions, and kits for generating nucleic acid products substantially free of template nucleic acid
WO2014093330A1 (en) * 2012-12-10 2014-06-19 Clearfork Bioscience, Inc. Methods for targeted genomic analysis
WO2015039006A1 (en) * 2013-09-16 2015-03-19 The General Hospital Corporation Methods of treating cancer
WO2018111835A1 (en) * 2016-12-12 2018-06-21 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015200541A1 (en) * 2014-06-24 2015-12-30 Bio-Rad Laboratories, Inc. Digital pcr barcoding
WO2016081798A1 (en) * 2014-11-20 2016-05-26 Children's Medical Center Corporation Methods relating to the detection of recurrent and non-specific double strand breaks in the genome

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011019964A1 (en) * 2009-08-12 2011-02-17 Nugen Technologies, Inc. Methods, compositions, and kits for generating nucleic acid products substantially free of template nucleic acid
WO2014093330A1 (en) * 2012-12-10 2014-06-19 Clearfork Bioscience, Inc. Methods for targeted genomic analysis
WO2015039006A1 (en) * 2013-09-16 2015-03-19 The General Hospital Corporation Methods of treating cancer
WO2018111835A1 (en) * 2016-12-12 2018-06-21 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
QING WANG ET AL: "Targeted sequencing of both DNA strands barcoded and captured individually by RNA probes to identify genome-wide ultra-rare mutations", SCIENTIFIC REPORTS, vol. 7, no. 1, 13 June 2017 (2017-06-13), XP055551336, DOI: 10.1038/s41598-017-03448-8 *
SCOTT R KENNEDY ET AL: "Detecting ultralow-frequency mutations by Duplex Sequencing", NATURE PROTOCOLS, vol. 9, no. 11, 9 October 2014 (2014-10-09), GB, pages 2586 - 2606, XP055745195, ISSN: 1754-2189, DOI: 10.1038/nprot.2014.170 *
See also references of WO2020005159A1 *

Also Published As

Publication number Publication date
WO2020005159A1 (en) 2020-01-02
SG11202012687SA (en) 2021-01-28
CN112639127A (en) 2021-04-09
EP3810805A1 (en) 2021-04-28

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