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EP0272963B1 - Sampling and dosing pipette; process and device for its calibration - Google Patents

Sampling and dosing pipette; process and device for its calibration Download PDF

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Publication number
EP0272963B1
EP0272963B1 EP87402678A EP87402678A EP0272963B1 EP 0272963 B1 EP0272963 B1 EP 0272963B1 EP 87402678 A EP87402678 A EP 87402678A EP 87402678 A EP87402678 A EP 87402678A EP 0272963 B1 EP0272963 B1 EP 0272963B1
Authority
EP
European Patent Office
Prior art keywords
pipette
volume
sampling
knurled knob
samplings
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP87402678A
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German (de)
French (fr)
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EP0272963A1 (en
Inventor
Eric Marteau D'autry
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Individual
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Individual
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Publication date
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Priority to AT87402678T priority Critical patent/ATE97347T1/en
Publication of EP0272963A1 publication Critical patent/EP0272963A1/en
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Publication of EP0272963B1 publication Critical patent/EP0272963B1/en
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0224—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type having mechanical means to set stroke length, e.g. movable stops
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/025—Displaying results or values with integrated means
    • B01L2300/026—Drum counters

Definitions

  • the present invention relates to a method and a device for calibrating a sampling and dosing pipette as well as to a pipette allowing a fully automated implementation of the method which is the subject of the invention.
  • the sampling and dosing pipettes currently available on the market comprise a pipette body 1 allowing the display of the sampling volume.
  • the sample volume is determined by a piston movable in the pipette body and actuated by a central rod 5 and a return spring 6.
  • An axial adjustment screw 2 meshes with means 20 for displaying the sample volume, the screw adjustment being integral with a knurled button 3 allowing the adjustment of the piston stroke in the pipette body.
  • the central rod 5, and the piston on reaction of the return spring comes to rest in abutment with an adjustable stop in position 70 at the end of the knurled button 3 in order to allow the adjustment of the zero of the stroke of the central rod 5 and the piston, and, consequently, the sampling volume.
  • this calibration consisting in fact of setting or adjusting the zero of the sample volume.
  • the aforementioned calibration operations can hardly be carried out except manually, appreciably, and the production on an industrial scale of such types of pipettes implies, prior to their placing on the market, the implementation of a calibration station which is costly in terms of labor and material, in order to ensure sufficient production.
  • the essentially manual nature of the abovementioned calibration operation cannot make it possible to be entirely free from errors or faults in adjustment or calibration, precisely because of the human factor which is inevitably a source of errors and imprecision. , the reduction of this type of error requiring for example additional statistical control operations or the like.
  • the object of the present invention is to remedy the aforementioned drawbacks by implementing a method and a device for calibrating a sampling and dosing pipette as well as a pipette specially adapted for fully automated calibration.
  • Another object of the present invention is the implementation of a method and a device for calibrating a sampling and dosing pipette, in which human intervention is substantially eliminated, the method being carried out in an automated manner. using the device object of the invention.
  • Another object of the present invention is also the implementation of a method and a device for calibrating a sampling and dosing pipette allowing very high precision and very high homogeneity to be obtained. calibration on very large batches of pipettes subjected to the process.
  • a sampling and dosing pipette in accordance with the object of the invention will firstly be described in connection with FIG. 2a.
  • the sampling and metering pipette which is the subject of the invention comprises, in a similar manner to the pipette of the prior art, as shown in FIG. 1, a pipette body 1 with display of the sampling volume, an adjusting screw 2 meshing with means for displaying the sampling volume, the adjusting screw being integral with a knurled button 3 to allow the volume of a sampling chamber formed by a piston actuated by a central rod to be adjusted 5.
  • the piston 4 is movable in the pipette body 1 on action of the central rod and reaction of a return spring 6.
  • the central rod on reaction of the return spring 6 abuts at the end of the adjustment screw 2 comprising the knurled knob 3.
  • the stop is formed by a brake screw for adjusting the volume of the chamber to zero. sample.
  • the brake screw is adjustable in the translational position relative to the end of the adjustment screw 2 and to the knurled knob 3 by a rotational movement.
  • the brake screw comprises a threaded element 70 engaged in a thread provided in a housing of the knurled button 3.
  • a friction element 71 forming a brake against the wall of the housing is also provided.
  • mechanical means 72 are arranged on the external face of the threaded element 70, external face perpendicular to the axis ⁇ of the mechanical means of the threaded element constituting the worm which is at the same time the longitudinal arc of the pipette body.
  • the mechanical means 72 arranged on the external face of the threaded element 70, face perpendicular to the axis of the mechanical means of the threaded element 70, consist of a plurality of holes arranged for example according to a circular arrangement on the aforementioned external face. These holes are intended to allow the drive of the brake screw 70, 71 for adjustment, by means of a tool specially adapted for this purpose, which will be described later in the description.
  • the friction element 71 forming a brake can advantageously be disposed between the external face of the threaded element comprising the above-mentioned mechanical means 72 and a thread intended to be engaged in the tapping of the housing of the knurled button 3 as described above.
  • the friction element 71 can advantageously consist of a rubber ring for example or a ring of a flexible material, allowing the locking of the threaded element relative to the knurled button 3 in the absence of any setting torque. in rotation exerted on the threaded element 70.
  • the actuation of the knurled button 3 by the operator allows the operator to adjust the stroke of the central rod 5 and of the piston (not shown) and ultimately the choice of sampling volume defined by the stroke of the piston in the pipette body 1, stroke between the rest position of the piston and the end of the pipette body 1 not shown in the drawing.
  • the adjustment of the stroke of the piston and of the central rod 5 is thus carried out by screwing the adjusting screw 2, by means of the knurled button 3, relative to the pipette body 1, the assembly constituted by the knurled button 3, the adjusting screw 2, the central rod 5 and the piston thus moving in translation along the longitudinal axis of symmetry of the pipette.
  • the adjusting screw 2 meshing with the means for displaying the sampling volume, this volume is displayed directly in a window provided in the pipette body.
  • the zero setting of the sampling volume can be obtained by adjusting the relative position of the threaded element 70 adjustable in position, relative to the knurled knob 3 or adjusting screw 2.
  • the choice and the display of the sampling volume having been carried out by the operator, as described above, the latter can then actuate the central rod 5 by means of the push button 50, so as to bring the piston, not shown in FIG.
  • the spring 6 then allows, when the effort on the push button 50 is removed by the operator, the return of the assembly constituted by the piston and the central rod 5 to its initial position, the withdrawal of a quantity predetermined substance to be dosed thus being carried out.
  • this consists, firstly, of adjusting the volume of the sampling chamber to zero by means of the knurled knob 3, then of actuating the brake screw 70, 71 d 'adjustment, to substantially cancel the piston stroke.
  • VO - m a minimum withdrawal volume, denoted VO - m, corresponding to the position denoted m of the brake screw 70, 71, the latter being screwed to the maximum in the housing provided for this purpose in the knurled button 3.
  • VO sampling volume substantially zero, this being effected by adjusting the knurled button 3 , setting position of the brake screw 70, 71 for adjusting the previously defined position m, makes it possible to define a substantially zero piston stroke.
  • the volume of the sampling chamber is then adjusted to a value corresponding to a non-zero volume of sampling.
  • a plurality of samples of the aforementioned volume of a reference substance can then be taken.
  • a consistency test of the plurality of the abovementioned samples can then be carried out.
  • An average of the calculated values of the volumes of the plurality of abovementioned samples can then be taken, this average making it possible to define an average value of the sample volume.
  • a new adjustment of the brake screw 70, 71 is then carried out, the brake screw 70, 71 then being moved in rotation by a number of turns corresponding in value and as a sign of the difference between the value of the volume displayed. by the display means of the pipette body 1 and the aforementioned average value of the sample volume. It is understood that the adjustment of a number of turns corresponding in value and in sign to the difference between the value of the volume displayed and the aforementioned average value, can consist of a fraction of turns of screwing of the brake screw d 'adjustment 70, 71.
  • the pipette can advantageously be subjected to a control by multiple samples allowing either the acceptance of the pipette as a calibrated pipette, or the resumption of the proceeded to the previous step, step consisting in carrying out an average of the values of the volumes of the plurality of withdrawals.
  • the consistency test can consist in admitting each of the samples forming the plurality of samples if the latter is included in a dispersion range determined with respect to a reference value.
  • the values of the volumes of the plurality of multiple samples can advantageously be calculated by gravimetry.
  • the reference substance can consist of any non-volatile substance.
  • the pipette can be subjected to a calibration correction in accordance with the steps previously described in the description, steps consisting in carrying out, following a consistency test of the plurality of abovementioned samples, an average of these samples, then in carrying out a new adjustment of the adjustment brake screw 70, 71, by a corresponding number of turns in value and as a sign of the difference between the value of the volume displayed and the average value displayed.
  • the device which is the subject of the invention comprises at least means denoted 100 for driving the brake screw 70, 71 and the knurled knob 3.
  • Means denoted 300 for control are also provided, so as to allow the control of the means 100 for driving the brake screw 70, 71 and the knurled button 3.
  • the means 100 for driving the brake screw 70, 71 may advantageously include lugs 1020 intended to be mechanically coupled to the mechanical means 72 provided on the external face of the threaded element 70. These lugs allow the application of a rotation torque of the threaded element 70 constituting the brake screw 70, 71.
  • the lug pin 102 can be constituted by the head of the rod body 2101 in which the central rod 5 is engaged.
  • the rod body 2101 can advantageously be mounted movable in rotation relative to a part with ball bearing 2102.
  • the latter to constitute the drive means of the knurled button 3, also comprises lugs 2120 intended to come into engagement in corresponding orifices denoted 31, 32 of the knurled button 3, in order to immobilize or drive the knurled button 3, with the part 2102, prior to an adjustment operation, by screwing or unscrewing the brake screw 70, 71.
  • the drive means 100 may also comprise a drive motor 101 coupled by a clutch transmission of conventional type by belt by example, to the stem body 2101 and to the piece 2102.
  • control means 300 of the drive means 100 of the brake screw can be constituted by a microcomputer, provided with its peripherals.
  • Peripherals are understood, of course, to be permanent memory of the memory type with magnetic support, intercom keyboard with an operator and display monitor, allowing interactive dialogue between the operator and the entire system.
  • the implementation of the calibration method, object of the invention, using the device as shown in Figure 4 will now be described.
  • the pipette having been brought into position, vis-à-vis respectively of the lugs of the lug pin 102, of the lugs 2120 of the head of the piece 2102, firstly, the volume of the sampling chamber is adjusted to zero by means of the knurled button 3, by means of the motor 101 and the lugs 2120 of the part 2102, the latter having as their object to drive the knurled button 3.
  • the adjusting brake screw 70 is then actuated by means of the pin 102 with pins 1020, for substantially canceling the stroke of the piston.
  • the volume of the sampling chamber is adjusted by rotating the knurled button 3, by means of the motor 101 and of the part 2102 and of the lugs 2120, in order to define an average value of the sampling volume.
  • a new adjustment of the brake screw 70, 71 of adjustment is carried out by rotation thereof, by means of the motor 101 and of the lug pin 1020, of a corresponding number of turns in value and in sign, to the difference between the value of the volume displayed by the display means of the pipette body 1 and the average value of the abovementioned sample volume.
  • the method for calibrating the sampling and dosing pipette, object of the invention and the device allowing the implementation of this method are particularly remarkable in that they allow, with a low expenditure of material, very precise control. calibration of the sampling and dosing pipette, as well as a very high uniformity of calibration on a very large batch of pipettes intended for marketing.

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  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

The invention relates to a process and a device for calibrating a sampling and metering pipette and a pipette adapted to be used in the process with the device. The volume of the sampling chamber of the pipette is adjusted to zero by means of a knurled button (3) and an adjusting screw (7) is actuated to set the stroke of a plunger for taking a sample at a zero value. The volume of the sample chamber is adjusted by means of the knurled button (3) to determine a mean value of the sample volume, and a new adjustment of the brake screw (7) is made to a value corresponding to the difference between a displayed volume value and the mean value of the sample volume.

Description

La présente invention est relative à un procédé et un dispositif de calibrage d'une pipette de prélèvement et de dosage ainsi qu'à une pipette permettant une mise en oeuvre entièrement automatisée du procédé objet de l'invention.The present invention relates to a method and a device for calibrating a sampling and dosing pipette as well as to a pipette allowing a fully automated implementation of the method which is the subject of the invention.

Les pipettes de prélèvement et de dosage actuellement disponibles dans le commerce, ainsi que représenté en figure 1, comportent un corps de pipette 1 permettant l'affichage du volume de prélèvement. Le volume de prélèvement est déterminé par un piston mobile dans le corps de pipette et actionné par une tige centrale 5 et un ressort de rappel 6. Une vis de réglage axiale 2 engrène des moyens d'affichage 20 du volume de prélèvement, la vis de réglage étant solidaire d'un bouton moleté 3 permettant le réglage de la course du piston dans le corps de pipette. La tige centrale 5, et le piston sur réaction du ressort de rappel, vient au repos en butée d'une butée réglable en position 70 à l'extrémité du bouton moleté 3 afin de permettre le réglage du zéro de la course de la tige centrale 5 et du piston, et, en conséquence, du volume de prélèvement.The sampling and dosing pipettes currently available on the market, as shown in FIG. 1, comprise a pipette body 1 allowing the display of the sampling volume. The sample volume is determined by a piston movable in the pipette body and actuated by a central rod 5 and a return spring 6. An axial adjustment screw 2 meshes with means 20 for displaying the sample volume, the screw adjustment being integral with a knurled button 3 allowing the adjustment of the piston stroke in the pipette body. The central rod 5, and the piston on reaction of the return spring, comes to rest in abutment with an adjustable stop in position 70 at the end of the knurled button 3 in order to allow the adjustment of the zero of the stroke of the central rod 5 and the piston, and, consequently, the sampling volume.

Ce type de pipette a jusqu'à ce jour donné entière satisfaction tant du point de vue de la fiabilité d'utilisation que de la fidélité des mesures ou dosages répétitifs.This type of pipette has so far given complete satisfaction both from the point of view of reliability of use and of the precision of repetitive measurements or dosages.

Cependant, préalablement à la commercialisation de ce type d'appareil, il est nécessaire d'effectuer un calibrage à l'unité, ce calibrage consistant en fait en un réglage ou ajustement du zéro du volume de prélèvement.However, prior to the commercialization of this type of device, it is necessary to perform a unit calibration, this calibration consisting in fact of setting or adjusting the zero of the sample volume.

En raison de la structure propre du système de réglage ou ajustement du zéro du volume de prélèvement, les opérations de calibrage précédemment citées ne peuvent guère être menées à bien que manuellement,sensiblement,et la production à l'échelle industrielle de tels types de pipettes implique, préalablement à leur mise sur le marché, la mise en oeuvre d'un poste de calibrage coûteux en main d'oeuvre et en matériel, en vue d'assurer une production suffisante. En outre, le caractère essentiellement manuel de l'opération de calibrage précitée ne peut permettre de s'affranchir en totalité d'erreurs ou de défauts de réglage ou de calibrae, en raison précisément du facteur humain inévitablement source d'erreurs et d'imprécision, la réduction de ce type d'erreur nécessitant par exemple des opérations de contrôle statistique supplémentaires ou analogues.Due to the specific structure of the zero volume sampling adjustment or adjustment system, the aforementioned calibration operations can hardly be carried out except manually, appreciably, and the production on an industrial scale of such types of pipettes implies, prior to their placing on the market, the implementation of a calibration station which is costly in terms of labor and material, in order to ensure sufficient production. In addition, the essentially manual nature of the abovementioned calibration operation cannot make it possible to be entirely free from errors or faults in adjustment or calibration, precisely because of the human factor which is inevitably a source of errors and imprecision. , the reduction of this type of error requiring for example additional statistical control operations or the like.

La présente invention a pour but de remédier aux inconvénients précités par la mise en oeuvre d'un procédé et d'un dispositif de calibrage d'une pipette de prélèvement et de dosage ainsi que d'une pipette spécialement adaptée à un calibrage entièrement automatisé.The object of the present invention is to remedy the aforementioned drawbacks by implementing a method and a device for calibrating a sampling and dosing pipette as well as a pipette specially adapted for fully automated calibration.

Un autre objet de la présente invention est la mise en oeuvre d'un procédé et d'un dispositif de calibrage d'une pipette de prélèvement et de dosage, dans lesquels l'intervention humaine est sensiblement supprimée, le procédé étant conduit de manière automatisée à l'aide du dispositif objet de l'invention.Another object of the present invention is the implementation of a method and a device for calibrating a sampling and dosing pipette, in which human intervention is substantially eliminated, the method being carried out in an automated manner. using the device object of the invention.

Un autre objet de la présente invention est également la mise en oeuvre d'un procédé et d'un dispositif de calibrage d'une pipette de prélèvement et de dosage permettant l'obtention d'une très grande précision et d'une très grande homogénéité de calibrage sur des lots très importants de pipettes soumises au procédé.Another object of the present invention is also the implementation of a method and a device for calibrating a sampling and dosing pipette allowing very high precision and very high homogeneity to be obtained. calibration on very large batches of pipettes subjected to the process.

La pipette objet de l'invention est définie par la revendication 1.The pipette which is the subject of the invention is defined by claim 1.

Le procédé et le dispositif de calibrage d'une telle pipette de prélèvement et de dosage, objet de l'invention, sont définis par les revendications 2 et 6.The method and device for calibrating such a sampling and dosing pipette, which is the subject of the invention, are defined by claims 2 and 6.

L'invention sera mieux comprise à la lecture de la description et à l'observation des dessins ci-après, dans lesquels :

  • la figure 1 représente, en coupe selon un plan longitudinal de symétrie, une vue relative à une pipette de prélèvement et dosage de l'art antérieur,
  • la figure 2a représente, en coupe selon un plan longitudinal de symétrie, une vue relative à une pipette de prélèvement et dosage conformément à la présente invention,
  • la figure 2b représente une vue partielle de la figure 2a,
  • la figure 3 représente un schéma illustratif du procédé de calibrage, objet de l'invention,
  • la figure 4 représente en coupe un schéma illustratif d'un dispositif de calibrage, conformément à la présente invention.
The invention will be better understood on reading the description and on observing the drawings below, in which:
  • FIG. 1 represents, in section along a longitudinal plane of symmetry, a view relating to a prior art sampling and dosing pipette,
  • FIG. 2a represents, in section along a longitudinal plane of symmetry, a view relating to a sampling and dosing pipette according to the present invention,
  • FIG. 2b represents a partial view of FIG. 2a,
  • FIG. 3 represents an illustrative diagram of the calibration process, object of the invention,
  • Figure 4 shows in section an illustrative diagram of a calibration device, in accordance with the present invention.

Une pipette de prélèvement et de dosage conformément à l'objet de l'invention sera tout d'abord décrite en liaison avec la figure 2a.A sampling and dosing pipette in accordance with the object of the invention will firstly be described in connection with FIG. 2a.

Selon la figure précitée, la pipette de prélèvement et de dosage objet de l'invention comprend, de manière analogue à la pipette de l'art antérieur, telle que représentée en figure 1, un corps de pipette 1 à affichage du volume de prélèvement, une vis de réglage 2 engrenant des moyens d'affichage du volume de prélèvement, la vis de réglage étant solidaire d'un bouton moleté 3 pour permettre le réglage du volume d'une chambre de prélèvement formée d'un piston actionné par une tige centrale 5. Le piston 4 est mobile dans le corps de pipette 1 sur action de la tige centrale et réaction d'un ressort de rappel 6.According to the above-mentioned figure, the sampling and metering pipette which is the subject of the invention comprises, in a similar manner to the pipette of the prior art, as shown in FIG. 1, a pipette body 1 with display of the sampling volume, an adjusting screw 2 meshing with means for displaying the sampling volume, the adjusting screw being integral with a knurled button 3 to allow the volume of a sampling chamber formed by a piston actuated by a central rod to be adjusted 5. The piston 4 is movable in the pipette body 1 on action of the central rod and reaction of a return spring 6.

La tige centrale sur réaction du ressort de rappel 6 vient en butée au niveau de l'extrémité de la vis de réglage 2 comportant le bouton moleté 3. La butée est formée par une vis frein d'ajustement du zéro du volume de la chambre de prélèvement. La vis frein est réglable en position de translation par rapport à l'extrémité de la vis de réglage 2 et au bouton moleté 3 par un mouvement de rotation.The central rod on reaction of the return spring 6 abuts at the end of the adjustment screw 2 comprising the knurled knob 3. The stop is formed by a brake screw for adjusting the volume of the chamber to zero. sample. The brake screw is adjustable in the translational position relative to the end of the adjustment screw 2 and to the knurled knob 3 by a rotational movement.

La vis frein comprend un élément fileté 70 engagé dans un taraudage prévu dans un logement du bouton moleté 3. Un élément de friction 71 formant frein contre la paroi du logement est en outre prévu.The brake screw comprises a threaded element 70 engaged in a thread provided in a housing of the knurled button 3. A friction element 71 forming a brake against the wall of the housing is also provided.

En outre des moyens mécaniques 72 sont disposés sur la face externe de l'élément fileté 70, face externe perpendiculaire à l'axe Δ des moyens mécaniques de l'élément fileté constituant la vis sans fin qui est en même temps l'arc longitudinal du corps de pipette.In addition, mechanical means 72 are arranged on the external face of the threaded element 70, external face perpendicular to the axis Δ of the mechanical means of the threaded element constituting the worm which is at the same time the longitudinal arc of the pipette body.

Ainsi qu'il a été représenté en figure 2b, les moyens mécaniques 72 disposés sur la face externe de l'élément fileté 70, face perpendiculaire à l'axe des moyens mécaniques de l'élément fileté 70, consistent en une pluralité de trous disposés par exemple selon un arrangement circulaire sur la face externe précitée. Ces trous sont destinés à permettre l'entraînement de la vis frein 70, 71 d'ajustement, par l'intermédiaire d'un outil spécialement adapté à cet effet, lequel sera décrit ultérieurement dans la description.As shown in FIG. 2b, the mechanical means 72 arranged on the external face of the threaded element 70, face perpendicular to the axis of the mechanical means of the threaded element 70, consist of a plurality of holes arranged for example according to a circular arrangement on the aforementioned external face. These holes are intended to allow the drive of the brake screw 70, 71 for adjustment, by means of a tool specially adapted for this purpose, which will be described later in the description.

En outre, l'élément de friction 71 formant frein peut avantageusement être disposé entre la face externe de l'élément fileté comportant des moyens mécaniques 72 précités et un filetage destiné à être engagé dans le taraudage du logement du bouton moleté 3 ainsi que précédemment décrit. L'élément de friction 71 peut, avantageusement, être constitué par un anneau en caoutchouc par exemple ou un anneau en un matériau souple, permettant le blocage de l'élément fileté par rapport au bouton moleté 3 en l'absence de tout couple de mise en rotation exercé sur l'élément fileté 70.In addition, the friction element 71 forming a brake can advantageously be disposed between the external face of the threaded element comprising the above-mentioned mechanical means 72 and a thread intended to be engaged in the tapping of the housing of the knurled button 3 as described above. . The friction element 71 can advantageously consist of a rubber ring for example or a ring of a flexible material, allowing the locking of the threaded element relative to the knurled button 3 in the absence of any setting torque. in rotation exerted on the threaded element 70.

Un mode de réalisation avantageux du procédé de calibrage d'une pipette de prélèvement et de dosage conformément à l'invention sera décrit en liaison avec la figure 3.An advantageous embodiment of the method for calibrating a sampling and dosing pipette according to the invention will be described in connection with FIG. 3.

Préalablement à la description du procédé objet de l'invention, un rappel succint du fonctionnement de la pipette telle que représentée en figure 2a par exemple sera tout d'abord effectué.Prior to the description of the process which is the subject of the invention, a brief reminder of the operation of the pipette as shown in FIG. 2a for example will first be made.

L'actionnement du bouton moleté 3 par l'opérateur permet à celui-ci le réglage de la course de la tige centrale 5 et du piston (non représentés) et en définitive le choix du volume de prélèvement défini par la course du piston dans le corps de pipette 1, course comprise entre la position de repos du piston et l'extrémité du corps de pipette 1 non représentée au dessin. Le réglage de la course du piston et de la tige centrale 5 est ainsi effectué par vissage de la vis de réglage 2,par l'intermédiaire du bouton moleté 3, par rapport au corps de pipette 1, l'ensemble constitué par le bouton moleté 3, la vis de réglage 2, la tige centrale 5 et le piston se déplaçant ainsi en translation selon l'axe longitudinal de symétrie de la pipette. En outre, la vis de réglage 2 engrènant les moyens d'affichage du volume de prélèvement, ce volume est affiché directement dans une fenêtre ménagée dans le corps de pipette. On comprendra bien entendu que le réglage du zéro du volume de prélèvement peut être obtenu par le réglage de la position relative de l'élément fileté 70 réglable en position, par rapport au bouton moleté 3 ou vis de réglage 2. Le choix et l'affichage du volume de prélèvement ayant été effectués par l'opérateur, ainsi que décrit précédemment, celui-ci peut alors actionner la tige centrale 5 par l'intermédiaire du bouton poussoir 50, de façon à amener le piston, non représenté en figure 1, en position de butée inférieure, le ressort de rappel 6 permet alors, lors de la suppression de l'effort sur le bouton poussoir 50 par l'opérateur,le retour de l'ensemble constitué par le piston et la tige centrale 5 en sa position initiale, le prélèvement d'une quantité prédéterminée de la substance à doser étant ainsi effectué.The actuation of the knurled button 3 by the operator allows the operator to adjust the stroke of the central rod 5 and of the piston (not shown) and ultimately the choice of sampling volume defined by the stroke of the piston in the pipette body 1, stroke between the rest position of the piston and the end of the pipette body 1 not shown in the drawing. The adjustment of the stroke of the piston and of the central rod 5 is thus carried out by screwing the adjusting screw 2, by means of the knurled button 3, relative to the pipette body 1, the assembly constituted by the knurled button 3, the adjusting screw 2, the central rod 5 and the piston thus moving in translation along the longitudinal axis of symmetry of the pipette. In addition, the adjusting screw 2 meshing with the means for displaying the sampling volume, this volume is displayed directly in a window provided in the pipette body. It will of course be understood that the zero setting of the sampling volume can be obtained by adjusting the relative position of the threaded element 70 adjustable in position, relative to the knurled knob 3 or adjusting screw 2. The choice and the display of the sampling volume having been carried out by the operator, as described above, the latter can then actuate the central rod 5 by means of the push button 50, so as to bring the piston, not shown in FIG. 1, in the lower stop position, the spring 6 then allows, when the effort on the push button 50 is removed by the operator, the return of the assembly constituted by the piston and the central rod 5 to its initial position, the withdrawal of a quantity predetermined substance to be dosed thus being carried out.

Conformément à un aspect du procédé objet de l'invention, celui-ci consiste, dans un premier temps, à régler le volume de la chambre de prélèvement à zéro au moyen du bouton moleté 3, puis à actionner la vis frein 70, 71 d'ajustement, pour annuler sensiblement la course du piston. Sur le diagramme représenté en figure 3, on comprendra que pour un réglage du volume de prélèvement noté VO effectué au moyen du bouton moleté 3, le réglage de la vis frein 70, 71 d'ajustement en position, selon l'axe longitudinal du corps de pipette noté Δ, correspondant en fait à un réglage de position de la butée de l'équipage constitué par la tige centrale 5 et le piston, permet en fait d'obtenir un réglage effectif du volume de prélèvement VO, compris entre VO + M pour une position extrême saillante de la vis frein 70, 71 par rapport au bouton moleté 3, volume noté VO + M sur le diagramme de la figure 3, et un volume de prélèvement minimum, noté VO - m, correspondant à la position notée m de la vis frein 70, 71, celle-ci étant vissée au maximum dans le logement ménagé à cet effet dans le bouton moleté 3. Pour un choix du volume de prélèvement VO sensiblement nul, celui-ci étant effectué par réglage du bouton moleté 3, la mise en position de la vis frein 70, 71 d'ajustement en position m précédemment définie, permet de définir une course de piston sensiblement nulle.In accordance with one aspect of the process which is the subject of the invention, this consists, firstly, of adjusting the volume of the sampling chamber to zero by means of the knurled knob 3, then of actuating the brake screw 70, 71 d 'adjustment, to substantially cancel the piston stroke. On the diagram represented in FIG. 3, it will be understood that for an adjustment of the sampling volume denoted VO carried out by means of the knurled button 3, the adjustment of the brake screw 70, 71 of adjustment in position, along the longitudinal axis of the body pipette noted Δ, corresponding in fact to a position adjustment of the stop of the crew constituted by the central rod 5 and the piston, in fact makes it possible to obtain an effective adjustment of the sampling volume VO, between VO + M for an extreme projecting position of the brake screw 70, 71 relative to the knurled button 3, volume denoted VO + M on the diagram in FIG. 3, and a minimum withdrawal volume, denoted VO - m, corresponding to the position denoted m of the brake screw 70, 71, the latter being screwed to the maximum in the housing provided for this purpose in the knurled button 3. For a choice of the VO sampling volume substantially zero, this being effected by adjusting the knurled button 3 , setting position of the brake screw 70, 71 for adjusting the previously defined position m, makes it possible to define a substantially zero piston stroke.

Conformément au procédé objet de l'invention, le volume de la chambre de prélèvement est ensuite réglé sur une valeur correspondant à un volume non nul de prélèvement. Une pluralité de prélèvements du volume précité d'une substance de référence peut alors être effectuée.In accordance with the process which is the subject of the invention, the volume of the sampling chamber is then adjusted to a value corresponding to a non-zero volume of sampling. A plurality of samples of the aforementioned volume of a reference substance can then be taken.

Afin de garantir une grande fiabilité de calibrage du procédé objet de l'invention, un test de cohérence de la pluralité des prélèvements précités peut alors être effectué. Une moyenne des valeurs calculée des volumes de la pluralité des prélèvements précités peut alors être effectuée, cette moyenne permettant de définir une valeur moyenne du volume de prélèvement.In order to guarantee high reliability of calibration of the method which is the subject of the invention, a consistency test of the plurality of the abovementioned samples can then be carried out. An average of the calculated values of the volumes of the plurality of abovementioned samples can then be taken, this average making it possible to define an average value of the sample volume.

Un nouvel ajustement de la vis frein 70, 71 d'ajustement est alors effectué, la vis frein 70, 71 étant alors mue en rotation d'un nombre de tours correspondant en valeur et en signe à l'écart entre la valeur du volume affiché par les moyens d'affichage du corps de pipette 1 et la valeur moyenne du volume de prélèvement précitée. Il est bien entendu que l'ajustement d'un nombre de tours correspondant en valeur et en signe à l'écart entre la valeur du volume affiché et la valeur moyenne précitée, peut consister en une fraction de tours de vissage de la vis frein d'ajustement 70, 71.A new adjustment of the brake screw 70, 71 is then carried out, the brake screw 70, 71 then being moved in rotation by a number of turns corresponding in value and as a sign of the difference between the value of the volume displayed. by the display means of the pipette body 1 and the aforementioned average value of the sample volume. It is understood that the adjustment of a number of turns corresponding in value and in sign to the difference between the value of the volume displayed and the aforementioned average value, can consist of a fraction of turns of screwing of the brake screw d 'adjustment 70, 71.

Selon une caractéristique du procédé objet de l'invention précédemment décrit, suite à l'étape consistant à effectuer le nouvel ajustement de la vis frein 70, 71 d'ajustement, la pipette peut avantageusement être soumise à un contrôle par prélèvements multiples permettant soit l'acceptation de la pipette comme pipette calibrée, soit la reprise du procédé à l'étape précédente, étape consistant à effectuer une moyenne des valeurs des volumes de la pluralité des prélèvements.According to a characteristic of the method which is the subject of the invention previously described, following the step consisting in carrying out the new adjustment of the adjusting screw 70, 71, the pipette can advantageously be subjected to a control by multiple samples allowing either the acceptance of the pipette as a calibrated pipette, or the resumption of the proceeded to the previous step, step consisting in carrying out an average of the values of the volumes of the plurality of withdrawals.

Selon une caractéristique du procédé de l'invention, le test de cohérence peut consister à admettre chacun des prélèvements formant la pluralité de prélèvements si celui-ci est compris dans une plage de dispersion déterminée par rapport à une valeur de référence. Les valeurs des volumes de la pluralité des prélèvements multiples peuvent avantageusement être calculées par gravimétrie.According to a characteristic of the process of the invention, the consistency test can consist in admitting each of the samples forming the plurality of samples if the latter is included in a dispersion range determined with respect to a reference value. The values of the volumes of the plurality of multiple samples can advantageously be calculated by gravimetry.

La substance de référence peut être constituée par toute substance non volatile.The reference substance can consist of any non-volatile substance.

Dans le cas où l'écart entre la valeur du volume affiché et la moyenne des valeurs des multiples prélèvements est supérieure à une valeur de tolérance déterminée, la pipette peut être soumise à une correction de calibrage conformément aux étapes précédemment décrites dans la description, étapes consistant à effectuer, suite à un test de cohérence de la pluralité de prélèvements précités, une moyenne de ces prélèvements, puis à effectuer un nouvel ajustement de la vis frein d'ajustement 70, 71, d'un nombre de tours correspondant en valeur et en signe à l'écart entre la valeur du volume affiché et la valeur moyenne affichée.In the case where the difference between the value of the displayed volume and the average of the values of the multiple samples is greater than a determined tolerance value, the pipette can be subjected to a calibration correction in accordance with the steps previously described in the description, steps consisting in carrying out, following a consistency test of the plurality of abovementioned samples, an average of these samples, then in carrying out a new adjustment of the adjustment brake screw 70, 71, by a corresponding number of turns in value and as a sign of the difference between the value of the volume displayed and the average value displayed.

Un dispositif de calibrage d'une pipette de prélèvement et de dosage selon le procédé objet de l'invention sera maintenant décrit en liaison avec la figure 4.A device for calibrating a sampling and dosing pipette according to the process which is the subject of the invention will now be described in connection with FIG. 4.

Conformément à cette figure, le dispositif objet de l'invention comporte au moins des moyens notés 100 d'entraînement de la vis frein 70, 71 et du bouton moleté 3. Des moyens notés 300 de commande sont en outre prévus, de façon à permettre la commande des moyens 100 d'entraînement de la vis frein 70, 71 et du bouton moleté 3. Les moyens 100 d'entraînement de la vis frein 70, 71 peuvent comporter avantageusement des ergots 1020 destinés à s'accoupler mécaniquement aux moyens mécaniques 72 prévus sur la face externe de l'élément fileté 70. Ces ergots permettent l'application d'un couple de mise en rotation de l'élément fileté 70 constituant la vis frein 70, 71. De manière avantageuse, la broche à ergots 102 peut être constituée par la tête du corps de tige 2101 dans lequel est engagée la tige centrale 5. Le corps de tige 2101 peut, de manière avantageuse, être monté mobile en rotation par rapport à une pièce à roulement à billes 2102. Cette dernière, pour constituer les moyens d'entraînement du bouton moleté 3, comporte également des ergots 2120 destinés à venir s'engager dans des orifices correspondants notés 31, 32 du bouton moleté 3, afin d'immobiliser ou d'entraîner le bouton moleté 3, avec la pièce 2102, préalablement à une opération de réglage, par vissage ou dévissage de la vis frein 70, 71. Les moyens d'entraînement 100 peuvent en outre, comprendre un moteur d'entraînement 101 couplé par une transmission à embrayage de type classique par courroie par exemple, au corps de tige 2101 et à la pièce 2102.According to this figure, the device which is the subject of the invention comprises at least means denoted 100 for driving the brake screw 70, 71 and the knurled knob 3. Means denoted 300 for control are also provided, so as to allow the control of the means 100 for driving the brake screw 70, 71 and the knurled button 3. The means 100 for driving the brake screw 70, 71 may advantageously include lugs 1020 intended to be mechanically coupled to the mechanical means 72 provided on the external face of the threaded element 70. These lugs allow the application of a rotation torque of the threaded element 70 constituting the brake screw 70, 71. Advantageously, the lug pin 102 can be constituted by the head of the rod body 2101 in which the central rod 5 is engaged. The rod body 2101 can advantageously be mounted movable in rotation relative to a part with ball bearing 2102. The latter, to constitute the drive means of the knurled button 3, also comprises lugs 2120 intended to come into engagement in corresponding orifices denoted 31, 32 of the knurled button 3, in order to immobilize or drive the knurled button 3, with the part 2102, prior to an adjustment operation, by screwing or unscrewing the brake screw 70, 71. The drive means 100 may also comprise a drive motor 101 coupled by a clutch transmission of conventional type by belt by example, to the stem body 2101 and to the piece 2102.

Ainsi qu'il a été représenté schématiquement en figure 4, les moyens de commande 300 des moyens d'entraînement 100 de la vis frein peuvent être constitués par un microordinateur, muni de ses périphériques. Par périphériques, on entend bien entendu, mémoire permanentes de type mémoire à support magnétique, clavier d'intercommunication avec un opérateur et moniteur d'affichage, permettant un dialogue interactif entre l'opérateur et l'ensemble du système. La mise en oeuvre du procédé de calibrage, objet de l'invention, à l'aide du dispositif tel que représenté en figure 4 sera maintenant décrite. La pipette ayant été amenée en position, vis-à-vis respectivement des ergots de la broche à ergots 102, des ergots 2120 de la tête de la pièce 2102, dans un premier temps, le volume de la chambre de prélèvement est réglé à zéro au moyen du bouton moleté 3, par l'intermédiaire du moteur 101 et des ergots 2120 de la pièce 2102, ces derniers ayant pour objet d'entraîner le bouton moleté 3. La vis frein 70 d'ajustement est ensuite actionnée au moyen de la broche 102 à ergots 1020, pour annuler sensiblement la course du piston. Le volume de prélèvements minimum noté Vo-Vm, correspondant à la position m de la vis frein 70 vissée au maximum dans son logement ménagé dans le bouton moleté 3, pour un volume Vo sensiblement nul, permet de définir une course du piston sensiblement nulle.As shown schematically in Figure 4, the control means 300 of the drive means 100 of the brake screw can be constituted by a microcomputer, provided with its peripherals. Peripherals are understood, of course, to be permanent memory of the memory type with magnetic support, intercom keyboard with an operator and display monitor, allowing interactive dialogue between the operator and the entire system. The implementation of the calibration method, object of the invention, using the device as shown in Figure 4 will now be described. The pipette having been brought into position, vis-à-vis respectively of the lugs of the lug pin 102, of the lugs 2120 of the head of the piece 2102, firstly, the volume of the sampling chamber is adjusted to zero by means of the knurled button 3, by means of the motor 101 and the lugs 2120 of the part 2102, the latter having as their object to drive the knurled button 3. The adjusting brake screw 70 is then actuated by means of the pin 102 with pins 1020, for substantially canceling the stroke of the piston. The minimum volume of samples noted Vo-Vm, corresponding to the position m of the brake screw 70 screwed at the maximum in its housing formed in the knurled button 3, for a volume Vo substantially zero, makes it possible to define a stroke of the piston substantially zero.

Puis le volume de la chambre de prélèvement est réglé par mise en rotation du bouton moleté 3, par l'intermédiaire du moteur 101 et de la pièce 2102 et des ergots 2120, afin de définir une valeur moyenne du volume de prélèvement.Then the volume of the sampling chamber is adjusted by rotating the knurled button 3, by means of the motor 101 and of the part 2102 and of the lugs 2120, in order to define an average value of the sampling volume.

Un nouvel ajustement de la vis frein 70, 71 d'ajustement est effectué par mise en rotation de celle-ci, au moyen du moteur 101 et de la broche à ergots 1020, d'un nombre de tours correspondant en valeur et en signe, à l'écart entre la valeur du volume affiché par les moyens d'affichage du corps de pipette 1 et la valeur moyenne du volume de prélèvement précitée.A new adjustment of the brake screw 70, 71 of adjustment is carried out by rotation thereof, by means of the motor 101 and of the lug pin 1020, of a corresponding number of turns in value and in sign, to the difference between the value of the volume displayed by the display means of the pipette body 1 and the average value of the abovementioned sample volume.

Le procédé de calibrage de pipette de prélèvement et de dosage, objet de l'invention et le dispositif permettant la mise en oeuvre de ce procédé, sont particulièrement remarquables en ce qu'ils permettent, moyennant une faible dépense en matériel, un contrôle très précis du calibrage de pipette de prélèvement et de dosage, ainsi qu'une très grande homogénéité de calibrage sur un lot très important de pipettes destinées à la commercialisation.The method for calibrating the sampling and dosing pipette, object of the invention and the device allowing the implementation of this method, are particularly remarkable in that they allow, with a low expenditure of material, very precise control. calibration of the sampling and dosing pipette, as well as a very high uniformity of calibration on a very large batch of pipettes intended for marketing.

Claims (8)

  1. Sampling and dispensing pipette comprising a pipette body (1) with a display of the sampling volume, an adjustment screw (2) engaging with the means of displaying the said sampling volume, the adjustment screw being fixed to a knurled knob (3) so as to make it possible to adjust the volume of a sampling chamber partially defined by a piston able to move in the body of the pipette (1) and actuated by a central rod (5) and a return spring (6), the central rod (5), when acted on by the return spring, coming, at the end of the adjustment screw (2) comprising the knurled knob (3), into contact with a stop formed by a threaded component (70) for adjusting the sampling chamber, characterised in that the said threaded component (70) is engaged in a tapping provided in a recess in the knurled knob (3) with the interposing of a friction member (71) forming a restraint against the wall of the said recess, and in that means (72) for driving the said threaded component (70) are provided, on its external face perpendicular to the longitudinal axis Δ of the pipette body (1), in the form of a plurality of holes, so as to allow an adjustment in translational position with respect to the end of the adjustment screw and the knurled knob (3) by means of a rotational movement in order to enable the volume of the pipette sampling chamber to be adjusted.
  2. Method for automatically calibrating a sampling and dispensing pipette as defined in Claim 1, characterised in that the said method consists of:
    a) adjusting the volume of the sampling chamber to zero by acting on the knurled knob (3) of the pipette (1), then actuating the threaded adjusting component (70) by acting on a plurality of holes (72) provided on the external face perpendicular to the longitudinal axis Δ of the body of the pipette (1) in order to reduce the stroke of the piston substantially to zero,
    b) adjusting, by acting on the knurled knob (3), the volume of the sampling chamber to a small, non-zero volume, and then effecting a plurality of samplings of the aforesaid volume of a reference substance,
    c) taking, following a test of consistency of all the aforesaid samplings, an average of the calculated values of the volumes of the aforesaid plurality of samplings,
    d) effecting, still by acting on the said plurality of holes (72), a further adjustment of the threaded adjusting component (70) by a number of turns corresponding in value and sign to the difference between the value of the displayed volume and the aforesaid average value.
  3. Method according to Claim 2, characterised in that, following step d) consisting of effecting the further adjustment of the threaded adjusting component (70), the said pipette is subjected to a check by means of multiple samplings so that either the pipette will be accepted as a calibrated pipette or the process will be resumed at step c) above.
  4. Method according to one of Claims 2 or 3, characterised in that the consistency test consists of accepting each of the samplings forming the plurality of samplings if it lies within a dispersion range determined with respect to a reference value.
  5. Method according to one of Claims 2 to 4, characterised in that the values of the volumes of the plurality of multiple samplings are calculated gravimetrically.
  6. Device for calibrating a sampling and dispensing pipette according to the calibration method of one of Claims 2 to 5, characterised in that it includes at least:
    - a drive motor (101),
    - a spindle with pins (102) connected to the drive motor, the said pins constituting the male part cooperating with the holes (72) in the threaded component (70),
    - a part (2102) able to move in rotation about the spindle with pins (102), the said part (2102) itself being connected to the drive motor (101) and provided with pins (2120) intended to engage in corresponding orifices (31, 32) in the knurled knob (3) in order to actuate the latter,
    - means (300) for controlling the said means of driving the threaded component (70) and knurled knob (3).
  7. Device according to Claim 6, characterised in that the said control means (300) consist of a microprocessor together with its peripherals.
  8. Device according to Claim 7, characterised in that the said microcomputer has a permanent memory in which the commands for controlling the device for implementing the method according to Claims 2 to 5 are stored.
EP87402678A 1986-11-27 1987-11-26 Sampling and dosing pipette; process and device for its calibration Expired - Lifetime EP0272963B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AT87402678T ATE97347T1 (en) 1986-11-27 1987-11-26 DOSING AND SAMPLE PIPETTE; METHOD AND DEVICE FOR THEIR CALIBRATION.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR8616574 1986-11-27
FR8616574A FR2607407B1 (en) 1986-11-27 1986-11-27 METHOD AND DEVICE FOR CALIBRATING A PIPETTE FOR SAMPLING AND DOSING

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EP0272963A1 EP0272963A1 (en) 1988-06-29
EP0272963B1 true EP0272963B1 (en) 1993-11-18

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EP (1) EP0272963B1 (en)
JP (1) JPS63141650A (en)
AT (1) ATE97347T1 (en)
DE (1) DE3788198T2 (en)
FR (1) FR2607407B1 (en)

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US4672857A (en) * 1985-09-10 1987-06-16 Labindustries, Inc. Liquid microdispenser

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ATE97347T1 (en) 1993-12-15
DE3788198D1 (en) 1993-12-23
US4790176A (en) 1988-12-13
DE3788198T2 (en) 1994-06-16
JPH0349616B2 (en) 1991-07-30
EP0272963A1 (en) 1988-06-29
JPS63141650A (en) 1988-06-14
FR2607407B1 (en) 1991-08-02
FR2607407A1 (en) 1988-06-03

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