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EA201300876A1 - Мутантная целлобиогидролаза - Google Patents

Мутантная целлобиогидролаза

Info

Publication number
EA201300876A1
EA201300876A1 EA201300876A EA201300876A EA201300876A1 EA 201300876 A1 EA201300876 A1 EA 201300876A1 EA 201300876 A EA201300876 A EA 201300876A EA 201300876 A EA201300876 A EA 201300876A EA 201300876 A1 EA201300876 A1 EA 201300876A1
Authority
EA
Eurasian Patent Office
Prior art keywords
mutant
biohydrolase
stone
seq
sequence
Prior art date
Application number
EA201300876A
Other languages
English (en)
Other versions
EA023945B1 (ru
Inventor
Ян Метске Лан Ван Дер
Маргот Элизабет Франсуаз Схоневелд-Бергманс
Денисе Ильсе Якобс
Original Assignee
ДСМ АйПи АССЕТС Б.В.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ДСМ АйПи АССЕТС Б.В. filed Critical ДСМ АйПи АССЕТС Б.В.
Publication of EA201300876A1 publication Critical patent/EA201300876A1/ru
Publication of EA023945B1 publication Critical patent/EA023945B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Изобретение относится к мутантной целлобиогидролазе, являющейся мутантом SEQ ID NO:1, которая содержит замены в положении N247(I, F, H, W) в SEQ ID NO:1, которая идентична по меньшей мере на 50% последовательности SEQ ID NO:1 и которая имеет активность CBHI.
EA201300876A 2011-01-31 2012-01-30 Мутантная целлобиогидролаза EA023945B1 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161437804P 2011-01-31 2011-01-31
EP11152691 2011-01-31
PCT/EP2012/051416 WO2012104239A1 (en) 2011-01-31 2012-01-30 Mutant cellobiohydrolase

Publications (2)

Publication Number Publication Date
EA201300876A1 true EA201300876A1 (ru) 2013-12-30
EA023945B1 EA023945B1 (ru) 2016-07-29

Family

ID=44140893

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201300876A EA023945B1 (ru) 2011-01-31 2012-01-30 Мутантная целлобиогидролаза

Country Status (8)

Country Link
US (1) US8790894B2 (ru)
EP (1) EP2670845A1 (ru)
CN (1) CN103348005B (ru)
BR (1) BR112013019514A2 (ru)
CA (1) CA2824494A1 (ru)
EA (1) EA023945B1 (ru)
MX (1) MX340248B (ru)
WO (1) WO2012104239A1 (ru)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112013019039A2 (pt) 2011-01-26 2020-07-21 Novozymes A/S Célula hospedeira microbiana transgênica, métodos para produzir um polipeptídeo, para produzir um mutante de uma célula originária, para produzir uma proteína, para degradar um material celulósico, para produzir um produto de fermentação, 5 e parafermentar um material celulósico, construção de ácido nucleico ou vetor de expressão, polipeptídeo isolado, e polinucleotídeo isolado
EP2668269B1 (en) 2011-01-26 2018-01-03 Novozymes A/S Polypeptides having endoglucanase activity and polynucleotides encoding same
MX2015007233A (es) * 2012-12-12 2015-10-29 Danisco Us Inc Variantes de celobiohidrolasas.
WO2014138672A1 (en) 2013-03-08 2014-09-12 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
JP6441330B2 (ja) 2014-05-27 2018-12-19 国立研究開発法人産業技術総合研究所 熱安定性が改善されたセロビオハイドロラーゼ
WO2016037096A1 (en) 2014-09-05 2016-03-10 Novozymes A/S Carbohydrate binding module variants and polynucleotides encoding same
US10676769B2 (en) 2014-09-08 2020-06-09 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
JP6378622B2 (ja) 2014-12-04 2018-08-22 本田技研工業株式会社 高活性セロビオヒドロラーゼ
DK3262165T3 (da) 2015-02-24 2020-08-24 Novozymes As Cellobiohydrolasevarianter og polynukleotider, som koder for dem
CN105176949B (zh) * 2015-10-21 2018-07-13 青岛蔚蓝生物集团有限公司 一种纤维二糖水解酶突变体
CN109337888B (zh) * 2018-11-12 2021-08-17 南开大学 纤维二糖水解酶突变体及其生产方法和应用
CN110923219B (zh) * 2019-12-27 2022-08-12 荣成市慧海创达生物科技有限公司 纤维二糖水解酶的突变体

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5811381A (en) * 1996-10-10 1998-09-22 Mark A. Emalfarb Cellulase compositions and methods of use
US8637293B2 (en) * 1999-07-13 2014-01-28 Alliance For Sustainable Energy, Llc Cellobiohydrolase I enzymes
EP2295559B1 (en) * 2002-08-16 2015-09-16 Danisco US Inc. Novel variant Hyprocrea jecorina CBH1 cellulases with increase thermal stability comprising substitution or deletion at position T255
CA2509891C (en) 2002-12-19 2016-08-16 Dsm Ip Assets B.V. Novel food production process
US7271244B2 (en) * 2004-02-06 2007-09-18 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2006077258A1 (en) 2005-01-24 2006-07-27 Dsm Ip Assets B.V. Method for producing a compound of interest in a filamentous fungal cell
WO2010005553A1 (en) * 2008-07-07 2010-01-14 Mascoma Corporation Isolation and characterization of schizochytrium aggregatum cellobiohydrolase i (cbh 1)
AU2010240861B2 (en) 2009-04-24 2015-02-05 Dsm Ip Assets B.V. Carbohydrate degrading polypeptide and uses thereof

Also Published As

Publication number Publication date
CN103348005B (zh) 2015-03-18
US8790894B2 (en) 2014-07-29
WO2012104239A1 (en) 2012-08-09
EP2670845A1 (en) 2013-12-11
EA023945B1 (ru) 2016-07-29
MX2013008766A (es) 2014-01-31
MX340248B (es) 2016-07-01
US20130309729A1 (en) 2013-11-21
CA2824494A1 (en) 2012-08-09
CN103348005A (zh) 2013-10-09
BR112013019514A2 (pt) 2017-03-28

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Legal Events

Date Code Title Description
MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG MD TJ TM

MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): RU