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DK567888A - PROCEDURE FOR THE PRODUCTION OF PROTEINS - Google Patents

PROCEDURE FOR THE PRODUCTION OF PROTEINS

Info

Publication number
DK567888A
DK567888A DK567888A DK567888A DK567888A DK 567888 A DK567888 A DK 567888A DK 567888 A DK567888 A DK 567888A DK 567888 A DK567888 A DK 567888A DK 567888 A DK567888 A DK 567888A
Authority
DK
Denmark
Prior art keywords
acid
protein
production
anhydromevalonolactone
hydroxy
Prior art date
Application number
DK567888A
Other languages
Danish (da)
Other versions
DK567888D0 (en
DK175411B1 (en
Inventor
Andrea Stecher-Schilling
Rolf-Guenter Werner
William Werz
Hans Zaehner
Axel Zeeck
Original Assignee
Thomae Gmbh Dr K
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19873734632 external-priority patent/DE3734632A1/en
Priority claimed from DE19873738649 external-priority patent/DE3738649A1/en
Priority claimed from DE19883801562 external-priority patent/DE3801562A1/en
Application filed by Thomae Gmbh Dr K filed Critical Thomae Gmbh Dr K
Publication of DK567888D0 publication Critical patent/DK567888D0/en
Publication of DK567888A publication Critical patent/DK567888A/en
Application granted granted Critical
Publication of DK175411B1 publication Critical patent/DK175411B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

A method for increasing the production of protein, especially t-PA, in cultures of cells producing this protein, where a substance which induces the production of the protein is added to the cell culture, is characterised in that thioglycolic acid, thiodiglycolic acid, L-cysteine, glutathione, butyrylcholine bromide, butyrylcholine chloride, nonactic acid, furan fatty acid, ascorbic acid, aphidicolin, 6-hydroxy-4,6-dimethyl-3-hepten-2-one, fusaric acid, mevalonic acid, trans-anhydromevalonic acid, anhydromevalonolactone, cis-anhydromevalonolactone, D- alpha -hydroxy-substituted (C3 or C4) aliphatic mono- or dicarboxylic acid or the salts thereof is used as protein-inducing substance.
DK198805678A 1987-10-13 1988-10-12 Process for producing proteins DK175411B1 (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
DE3734632 1987-10-13
DE19873734632 DE3734632A1 (en) 1987-10-13 1987-10-13 Process for the preparation of t-PA
DE3738649 1987-11-13
DE19873738649 DE3738649A1 (en) 1987-11-13 1987-11-13 Process for the preparation of proteins
DE3801562 1988-01-20
DE19883801562 DE3801562A1 (en) 1988-01-20 1988-01-20 Process for the preparation of proteins

Publications (3)

Publication Number Publication Date
DK567888D0 DK567888D0 (en) 1988-10-12
DK567888A true DK567888A (en) 1989-04-14
DK175411B1 DK175411B1 (en) 2004-09-27

Family

ID=27196629

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198805678A DK175411B1 (en) 1987-10-13 1988-10-12 Process for producing proteins

Country Status (16)

Country Link
EP (1) EP0315782B1 (en)
JP (1) JP2688222B2 (en)
KR (1) KR890006671A (en)
AT (1) ATE112308T1 (en)
AU (1) AU614999B2 (en)
CA (1) CA1341400C (en)
DE (1) DE3851685D1 (en)
DK (1) DK175411B1 (en)
ES (1) ES2064336T3 (en)
FI (1) FI94963C (en)
HU (1) HU205381B (en)
IE (1) IE65986B1 (en)
IL (1) IL88001A (en)
NO (1) NO174778C (en)
NZ (1) NZ226521A (en)
PT (1) PT88751B (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE210150T1 (en) * 1991-04-18 2001-12-15 Toray Industries METHOD FOR PRODUCING INTERLEUKIN-6 COMPOSITIONS
DE4113750A1 (en) * 1991-04-26 1992-10-29 Boehringer Mannheim Gmbh IMPROVEMENT OF RENATURATION IN THE SECRETION OF DISULFID-BRIDGED PROTEINS
WO1998044146A1 (en) * 1997-04-03 1998-10-08 Yoshitomi Pharmaceutical Industries, Ltd. Process for producing foreign proteins
WO2003048357A1 (en) * 2001-12-04 2003-06-12 Mitsubishi Pharma Corporation Method of activating protein
JPWO2004056872A1 (en) * 2002-12-20 2006-04-20 三菱ウェルファーマ株式会社 Method for protecting thiol groups of proteins
ES2624185T3 (en) 2007-04-26 2017-07-13 Chugai Seiyaku Kabushiki Kaisha Method of cell culture using a medium enriched with amino acids

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3479814D1 (en) * 1984-06-05 1989-10-26 Asahi Chemical Ind Process for the preparation of a plasminogen activator
GB8606386D0 (en) * 1986-03-14 1986-04-23 Celltech Ltd Production of protein

Also Published As

Publication number Publication date
IL88001A0 (en) 1989-06-30
EP0315782A3 (en) 1989-09-20
NO174778C (en) 1994-07-06
HU205381B (en) 1992-04-28
FI94963C (en) 1995-11-27
DE3851685D1 (en) 1994-11-03
IE883079L (en) 1989-04-13
ES2064336T3 (en) 1995-02-01
NO174778B (en) 1994-03-28
AU614999B2 (en) 1991-09-19
FI884652A0 (en) 1988-10-11
EP0315782A2 (en) 1989-05-17
CA1341400C (en) 2002-11-19
NO884549D0 (en) 1988-10-12
JP2688222B2 (en) 1997-12-08
DK567888D0 (en) 1988-10-12
NO884549L (en) 1989-04-14
IL88001A (en) 1996-01-19
IE65986B1 (en) 1995-11-29
DK175411B1 (en) 2004-09-27
EP0315782B1 (en) 1994-09-28
PT88751A (en) 1989-07-31
FI94963B (en) 1995-08-15
KR890006671A (en) 1989-06-15
NZ226521A (en) 1991-04-26
FI884652A (en) 1989-04-14
HUT48306A (en) 1989-05-29
AU2373388A (en) 1989-05-11
JPH01231887A (en) 1989-09-18
ATE112308T1 (en) 1994-10-15
PT88751B (en) 1995-03-01

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