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DK3684930T3 - Modificering af ikke-kodende plante-RNA-molekylers specificitet for inaktivering af genekspression - Google Patents

Modificering af ikke-kodende plante-RNA-molekylers specificitet for inaktivering af genekspression Download PDF

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Publication number
DK3684930T3
DK3684930T3 DK18786034.1T DK18786034T DK3684930T3 DK 3684930 T3 DK3684930 T3 DK 3684930T3 DK 18786034 T DK18786034 T DK 18786034T DK 3684930 T3 DK3684930 T3 DK 3684930T3
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DK
Denmark
Prior art keywords
inactivation
specificity
modification
gene expression
rna molecules
Prior art date
Application number
DK18786034.1T
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English (en)
Inventor
Eyal Maori
Yaron Galanty
Cristina Pignocchi
Garcia Angela Chaparro
Ofir Meir
Original Assignee
Tropic Biosciences Uk Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB1715116.8A external-priority patent/GB201715116D0/en
Priority claimed from GBGB1715113.5A external-priority patent/GB201715113D0/en
Priority claimed from GBGB1719516.5A external-priority patent/GB201719516D0/en
Application filed by Tropic Biosciences Uk Ltd filed Critical Tropic Biosciences Uk Ltd
Application granted granted Critical
Publication of DK3684930T3 publication Critical patent/DK3684930T3/da

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    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
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    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
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    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
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  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
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DK18786034.1T 2017-09-19 2018-09-18 Modificering af ikke-kodende plante-RNA-molekylers specificitet for inaktivering af genekspression DK3684930T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GBGB1715116.8A GB201715116D0 (en) 2017-09-19 2017-09-19 Modifying the specificity of non-coding RNA molecules for silencing of target RNA in eukaryotic cells
GBGB1715113.5A GB201715113D0 (en) 2017-09-19 2017-09-19 Modifying the specificity of plant non-coding RNA molecules for silencing of target RNA
GBGB1719516.5A GB201719516D0 (en) 2017-11-23 2017-11-23 Modifying the specificity of plant non-coding RNA molecules for silencing of target RNA
PCT/IB2018/057160 WO2019058255A1 (en) 2017-09-19 2018-09-18 MODIFICATION OF THE SPECIFICITY OF VEGETABLE NON-CODANT RNA MOLECULES FOR THE SILENCING OF GENE EXPRESSION

Publications (1)

Publication Number Publication Date
DK3684930T3 true DK3684930T3 (da) 2022-06-07

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DK18786034.1T DK3684930T3 (da) 2017-09-19 2018-09-18 Modificering af ikke-kodende plante-RNA-molekylers specificitet for inaktivering af genekspression

Country Status (13)

Country Link
US (4) US20200270604A1 (da)
EP (3) EP3684929A1 (da)
JP (3) JP7462553B2 (da)
KR (3) KR20230050424A (da)
CN (2) CN111448318A (da)
AU (2) AU2018336128B2 (da)
BR (2) BR112020005444A2 (da)
CA (2) CA3074946A1 (da)
DK (1) DK3684930T3 (da)
ES (1) ES2915576T3 (da)
IL (3) IL313387A (da)
SG (2) SG11202002481RA (da)
WO (2) WO2019058253A1 (da)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112020005444A2 (pt) 2017-09-19 2020-09-29 Tropic Biosciences UK Limited modificação da especificidade de moléculas de rna não codificante de plantas para silenciar expressão gênica
CN112654710A (zh) 2018-05-16 2021-04-13 辛瑟高公司 用于指导rna设计和使用的方法和系统
CN110857438B (zh) * 2018-08-20 2022-05-17 中国烟草总公司黑龙江省公司牡丹江烟草科学研究所 一种高效产生siRNA的烟草花叶病毒基因片段、弱毒疫苗、制备方法及其应用
AU2020230897A1 (en) * 2019-03-01 2021-09-02 Syngenta Crop Protection Ag Suppression of target gene expression through genome editing of native miRNAs
GB201903521D0 (en) 2019-03-14 2019-05-01 Tropic Biosciences Uk Ltd No title
GB201903519D0 (en) * 2019-03-14 2019-05-01 Tropic Biosciences Uk Ltd Introducing silencing activity to dysfunctional rna molecules and modifying their specificity against a gene of interest
GB201903520D0 (en) * 2019-03-14 2019-05-01 Tropic Biosciences Uk Ltd Modifying the specificity of non-coding rna molecules for silencing genes in eukaryotic cells
CN110551719B (zh) * 2019-07-30 2023-06-02 中山大学 长链非编码rna基因alex1及其在提高水稻白叶枯病抗性中的应用
CN111508558B (zh) * 2020-03-23 2021-12-14 广州赛业百沐生物科技有限公司 一种基于CRISPR-Cas9技术设计点突变模型的方法及系统
CN111676220B (zh) * 2020-05-21 2022-03-29 扬州大学 杨树长链非编码RNA lnc11及其应用
WO2022006310A2 (en) * 2020-06-30 2022-01-06 Arizona Board Of Regents On Behalf Of The University Of Arizona Compositions for genome editing and methods of use thereof
CN114073760B (zh) * 2020-08-21 2024-02-13 北京大学 Rdr蛋白在肿瘤治疗中的应用
WO2022119881A1 (en) * 2020-12-01 2022-06-09 Emendobio Inc. Differential knockout of a heterozygous allele of lrrk2
CN112359049B (zh) * 2020-12-10 2022-01-28 昆明理工大学 一种岷江百合几丁质酶基因LrCHI2及其应用
CN112795568B (zh) * 2021-01-06 2023-07-18 河南农业大学 一种控制玉米雌穗穗粗杂种优势的非编码基因的应用及其前体序列
GB202103256D0 (en) 2021-03-09 2021-04-21 Tropic Biosciences Uk Ltd Method for silencing genes
CN114134155B (zh) * 2021-06-29 2023-09-12 中国农业科学院油料作物研究所 一种mlo基因突变体及其制备方法与应用
CN113444727B (zh) * 2021-06-30 2022-06-21 中国热带农业科学院热带生物技术研究所 一种LncRNA及其应用
JP2024524924A (ja) 2021-07-02 2024-07-09 トロピック バイオサイエンシーズ ユーケー リミテッド バナナ果実における褐変の遅延又は防止
KR102573947B1 (ko) * 2021-08-09 2023-09-01 경상국립대학교산학협력단 콩 유전자교정 효율 증대를 위한 유전자교정 시스템 및 이의 용도
KR102573952B1 (ko) * 2021-08-09 2023-09-01 경상국립대학교산학협력단 E2와 이의 상동체 동시 타겟 유전자교정 시스템 및 이의 용도
KR102584891B1 (ko) * 2021-08-09 2023-10-04 경상국립대학교산학협력단 GmIPK1 유전자교정 시스템 및 이의 용도
KR102573948B1 (ko) * 2021-08-09 2023-09-01 경상국립대학교산학협력단 Mips1과 이의 상동체 동시 타겟 유전자교정 시스템 및 이의 용도
KR102574819B1 (ko) * 2021-08-09 2023-09-04 경상국립대학교산학협력단 P34와 이의 상동체 동시 타겟 유전자교정 시스템 및 이의 용도
GB202112866D0 (en) 2021-09-09 2021-10-27 Tropic Biosciences Uk Ltd Resistance to fusarium wilt in a banana
GB202112865D0 (en) 2021-09-09 2021-10-27 Tropic Biosciences Uk Ltd Resistance to black sigatoka disease in banana
CN114807128B (zh) * 2022-03-24 2023-09-01 四川农业大学 一种lncRNA-BTRL及其应用
CN114794029B (zh) * 2022-06-09 2023-02-28 上海大学 一种计数秀丽隐杆线虫子宫内发育胚胎数目的方法
CN116064644B (zh) * 2022-10-10 2023-10-10 中国农业科学院生物技术研究所 SbAGO1b蛋白及其编码基因在调控植物抗虫性中的应用
CN115976050B (zh) * 2022-11-16 2024-06-18 西北农林科技大学 猕猴桃感病基因及其适用的vigs沉默体系构建
CN116103334A (zh) * 2022-11-16 2023-05-12 西北农林科技大学 一种猕猴桃抗溃疡病基因功能筛选专用瞬时转化体系
WO2024149907A2 (en) 2023-01-13 2024-07-18 Laverock Therapeutics Limited Novel polynucleotides, cells and methods
GB202305021D0 (en) 2023-04-04 2023-05-17 Tropic Biosciences Uk Ltd Methods for generating breaks in a genome
CN118581143B (zh) * 2024-08-02 2024-10-18 内蒙古农业大学 一个长链非编码RNA基因StlncFAD-AS在马铃薯抗病育种中的应用

Family Cites Families (73)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL154600B (nl) 1971-02-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen.
NL154598B (nl) 1970-11-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking.
NL154599B (nl) 1970-12-28 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking.
US3901654A (en) 1971-06-21 1975-08-26 Biological Developments Receptor assays of biologically active compounds employing biologically specific receptors
US3853987A (en) 1971-09-01 1974-12-10 W Dreyer Immunological reagent and radioimmuno assay
US3867517A (en) 1971-12-21 1975-02-18 Abbott Lab Direct radioimmunoassay for antigens and their antibodies
NL171930C (nl) 1972-05-11 1983-06-01 Akzo Nv Werkwijze voor het aantonen en bepalen van haptenen, alsmede testverpakkingen.
US3850578A (en) 1973-03-12 1974-11-26 H Mcconnell Process for assaying for biologically active molecules
US3935074A (en) 1973-12-17 1976-01-27 Syva Company Antibody steric hindrance immunoassay with two antibodies
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4034074A (en) 1974-09-19 1977-07-05 The Board Of Trustees Of Leland Stanford Junior University Universal reagent 2-site immunoradiometric assay using labelled anti (IgG)
US3984533A (en) 1975-11-13 1976-10-05 General Electric Company Electrophoretic method of detecting antigen-antibody reaction
US4098876A (en) 1976-10-26 1978-07-04 Corning Glass Works Reverse sandwich immunoassay
US4879219A (en) 1980-09-19 1989-11-07 General Hospital Corporation Immunoassay utilizing monoclonal high affinity IgM antibodies
CA1192510A (en) 1981-05-27 1985-08-27 Lawrence E. Pelcher Rna plant virus vector or portion thereof, a method of construction thereof, and a method of producing a gene derived product therefrom
JPS6054684A (ja) 1983-09-05 1985-03-29 Teijin Ltd 新規ｄνａ及びハイブリツドｄνａ
US5011771A (en) 1984-04-12 1991-04-30 The General Hospital Corporation Multiepitopic immunometric assay
US4666828A (en) 1984-08-15 1987-05-19 The General Hospital Corporation Test for Huntington's disease
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
CA1288073C (en) 1985-03-07 1991-08-27 Paul G. Ahlquist Rna transformation vector
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4801531A (en) 1985-04-17 1989-01-31 Biotechnology Research Partners, Ltd. Apo AI/CIII genomic polymorphisms predictive of atherosclerosis
GB8608850D0 (en) 1986-04-11 1986-05-14 Diatech Ltd Packaging system
JPS6314693A (ja) 1986-07-04 1988-01-21 Sumitomo Chem Co Ltd 植物ウイルスｒｎａベクタ−
ES2039474T3 (es) 1986-12-05 1993-10-01 Ciba-Geigy Ag Procedimineto mejorado para la transformacion de protoplastos vegetales.
US5015580A (en) 1987-07-29 1991-05-14 Agracetus Particle-mediated transformation of soybean plants and lines
DE3850683T2 (de) 1987-02-09 1994-10-27 Lubrizol Genetics Inc Hybrides RNS-Virus.
US5316931A (en) 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US5416011A (en) 1988-07-22 1995-05-16 Monsanto Company Method for soybean transformation and regeneration
US5693507A (en) 1988-09-26 1997-12-02 Auburn University Genetic engineering of plant chloroplasts
US5272057A (en) 1988-10-14 1993-12-21 Georgetown University Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for human poly (ADP-ribose) polymerase
US5302523A (en) 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
US7705215B1 (en) 1990-04-17 2010-04-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5550318A (en) 1990-04-17 1996-08-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5192659A (en) 1989-08-25 1993-03-09 Genetype Ag Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes
JP3209744B2 (ja) 1990-01-22 2001-09-17 デカルブ・ジェネティクス・コーポレーション 結実能力のある遺伝子変換コーン
US5484956A (en) 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
US6403865B1 (en) 1990-08-24 2002-06-11 Syngenta Investment Corp. Method of producing transgenic maize using direct transformation of commercially important genotypes
US5384253A (en) 1990-12-28 1995-01-24 Dekalb Genetics Corporation Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
DE69334225D1 (de) 1992-07-07 2008-07-31 Japan Tobacco Inc Verfahren zur transformation einer monokotyledon pflanze
US5281521A (en) 1992-07-20 1994-01-25 The Trustees Of The University Of Pennsylvania Modified avidin-biotin technique
AU670316B2 (en) 1992-07-27 1996-07-11 Pioneer Hi-Bred International, Inc. An improved method of (agrobacterium)-mediated transformation of cultured soybean cells
US5998193A (en) 1994-06-24 1999-12-07 Gene Shears Pty., Ltd. Ribozymes with optimized hybridizing arms, stems, and loops, tRNA embedded ribozymes and compositions thereof
US5693512A (en) 1996-03-01 1997-12-02 The Ohio State Research Foundation Method for transforming plant tissue by sonication
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
AU2848800A (en) 1999-01-14 2000-08-01 Monsanto Technology Llc Soybean transformation method
US9567591B2 (en) 2003-05-15 2017-02-14 Mello Biotechnology, Inc. Generation of human embryonic stem-like cells using intronic RNA
US20050203047A1 (en) * 2004-03-10 2005-09-15 Ulrich Thomann Delivery vectors for short interfering RNA, micro-RNA and antisense RNA
IL179285A (en) 2004-05-14 2011-04-28 Rosetta Genomics Ltd Micrornas and uses thereof
DK2650365T3 (da) 2005-10-18 2016-12-05 Prec Biosciences Rationelt konstruerede meganukleaser med ændret sekvensspecificitet og dna-bindingsaffinitet
EP1945765A2 (en) 2005-10-28 2008-07-23 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Structures of active guide rna molecules and method of selection
JP2010532159A (ja) 2007-06-07 2010-10-07 アグリカルチャー アンド アグリ−フード カナダ ナノキャリアに基づいたトランスフェクション及び形質導入植物
WO2009046384A1 (en) 2007-10-05 2009-04-09 Dow Agrosciences Llc Methods for transferring molecular substances into plant cells
US8212019B2 (en) * 2008-07-30 2012-07-03 University Of Massachusetts Nucleic acid silencing sequences
ES2710179T3 (es) 2009-09-07 2019-04-23 Nobel Biocare Services Ag Conjunto de implantación
EP2646549B1 (en) * 2010-12-02 2015-02-18 Keygene N.V. Targeted alteration of dna
AU2013225619A1 (en) 2012-02-29 2014-10-16 Benitec Biopharma Limited Pain treatment
SG11201505272QA (en) 2013-01-08 2015-08-28 Benitec Biopharma Ltd Age-related macular degeneration treatment
CA2917714A1 (en) 2013-08-07 2015-02-12 Regeneron Pharmaceuticals, Inc. Lincrna-deficient non-human animals
US20150082478A1 (en) 2013-08-22 2015-03-19 E I Du Pont De Nemours And Company Plant genome modification using guide rna/cas endonuclease systems and methods of use
JP2017527256A (ja) * 2013-12-12 2017-09-21 ザ・ブロード・インスティテュート・インコーポレイテッド ＨＢＶ及びウイルス性疾患及び障害のためのＣＲＩＳＰＲ−Ｃａｓ系及び組成物の送達、使用及び治療適用
ES2802984T3 (es) 2014-02-11 2021-01-22 Univ Colorado Regents Ingeniería del genoma multiplex mediante CRISPR
US20160076093A1 (en) 2014-08-04 2016-03-17 University Of Washington Multiplex homology-directed repair
KR102629128B1 (ko) 2014-12-03 2024-01-25 애질런트 테크놀로지스, 인크. 화학적 변형을 갖는 가이드 rna
WO2016100333A1 (en) * 2014-12-15 2016-06-23 Syngenta Participations Ag Pesticidal microrna carriers and use thereof
EP3303585A4 (en) 2015-06-03 2018-10-31 Board of Regents of the University of Nebraska Dna editing using single-stranded dna
CN106701752B (zh) 2015-09-01 2019-07-12 清华大学 人工合成的microRNA簇及其构建方法与所用合成microRNA单元
US12049625B2 (en) * 2016-01-14 2024-07-30 The Brigham And Women's Hospital, Inc. Genome editing for treating glioblastoma
CN105647962A (zh) * 2016-02-15 2016-06-08 浙江大学 运用CRISPR-Cas9系统敲除水稻MIRNA393b茎环序列的基因编辑方法
CN106367435B (zh) 2016-09-07 2019-11-08 电子科技大学 一种水稻miRNA定向敲除的方法
WO2018183878A1 (en) 2017-03-31 2018-10-04 Pioneer Hi-Bred International, Inc. Expression modulating elements and use thereof
BR112020005444A2 (pt) 2017-09-19 2020-09-29 Tropic Biosciences UK Limited modificação da especificidade de moléculas de rna não codificante de plantas para silenciar expressão gênica
AU2020230897A1 (en) 2019-03-01 2021-09-02 Syngenta Crop Protection Ag Suppression of target gene expression through genome editing of native miRNAs

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