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DK0466901T3 - DNA segments and transformed microorganisms comprising the delta-1 dehydrogenase gene and its uses - Google Patents

DNA segments and transformed microorganisms comprising the delta-1 dehydrogenase gene and its uses

Info

Publication number
DK0466901T3
DK0466901T3 DK91904078.2T DK91904078T DK0466901T3 DK 0466901 T3 DK0466901 T3 DK 0466901T3 DK 91904078 T DK91904078 T DK 91904078T DK 0466901 T3 DK0466901 T3 DK 0466901T3
Authority
DK
Denmark
Prior art keywords
delta
dna segments
dehydrogenase gene
transformed microorganisms
segments
Prior art date
Application number
DK91904078.2T
Other languages
Danish (da)
Inventor
Yvon Michel-Briand
Patrick Plesiat
Original Assignee
Roussel Uclaf
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roussel Uclaf filed Critical Roussel Uclaf
Application granted granted Critical
Publication of DK0466901T3 publication Critical patent/DK0466901T3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/99—Oxidoreductases acting on the CH-CH group of donors (1.3) with other acceptors (1.3.99)
    • C12Y103/99004—3-Oxosteroid 1-dehydrogenase (1.3.99.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The cloning of delta?1-dehydrogenase from Pseudomonas testosteroni, DNA segments comprising this gene, microorganisms transformed by the insertion of said segments and the use of said segments and microorganisms for producing delta?1-dehydrogenase and for synthesizing and assaying steroidal substances, are described.
DK91904078.2T 1990-02-07 1991-02-07 DNA segments and transformed microorganisms comprising the delta-1 dehydrogenase gene and its uses DK0466901T3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR909001399A FR2657882B1 (en) 1990-02-07 1990-02-07 DNA FRAGMENTS COMPRISING THE GENE OF 1-DEHYDROGENASE, MICROORGANISMS TRANSFORMED BY SAID FRAGMENTS AND APPLICATIONS OF SUCH FRAGMENTS AND MICROORGANISMS.

Publications (1)

Publication Number Publication Date
DK0466901T3 true DK0466901T3 (en) 1996-08-26

Family

ID=9393457

Family Applications (1)

Application Number Title Priority Date Filing Date
DK91904078.2T DK0466901T3 (en) 1990-02-07 1991-02-07 DNA segments and transformed microorganisms comprising the delta-1 dehydrogenase gene and its uses

Country Status (9)

Country Link
EP (1) EP0466901B1 (en)
JP (1) JPH04505105A (en)
AT (1) ATE140730T1 (en)
DE (1) DE69121024T2 (en)
DK (1) DK0466901T3 (en)
ES (1) ES2090312T3 (en)
FR (1) FR2657882B1 (en)
GR (1) GR3020614T3 (en)
WO (1) WO1991012326A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6020128A (en) * 1997-06-10 2000-02-01 United States Of Ameria DNA polymerase from Treponema pallidum
DE10204798A1 (en) * 2002-02-01 2003-08-14 Schering Ag Procedure for overexpression of dehydrogenases

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3734830A (en) * 1971-02-04 1973-05-22 Squibb & Sons Inc Continuous process for preparing delta-dehydrogenase

Also Published As

Publication number Publication date
EP0466901B1 (en) 1996-07-24
DE69121024D1 (en) 1996-08-29
JPH04505105A (en) 1992-09-10
ES2090312T3 (en) 1996-10-16
WO1991012326A1 (en) 1991-08-22
DE69121024T2 (en) 1997-01-16
EP0466901A1 (en) 1992-01-22
FR2657882A1 (en) 1991-08-09
GR3020614T3 (en) 1996-10-31
ATE140730T1 (en) 1996-08-15
FR2657882B1 (en) 1994-08-12

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