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DE69029785D1 - Kinetische Bestimmungsmethode für Endotoxin unter Verwendung von Limulus-Amoebocyten-Lysat und chromogenem Substrat - Google Patents

Kinetische Bestimmungsmethode für Endotoxin unter Verwendung von Limulus-Amoebocyten-Lysat und chromogenem Substrat

Info

Publication number
DE69029785D1
DE69029785D1 DE69029785T DE69029785T DE69029785D1 DE 69029785 D1 DE69029785 D1 DE 69029785D1 DE 69029785 T DE69029785 T DE 69029785T DE 69029785 T DE69029785 T DE 69029785T DE 69029785 D1 DE69029785 D1 DE 69029785D1
Authority
DE
Germany
Prior art keywords
endotoxin
chromogenic substrate
determination
assay procedure
amoebocyte lysate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69029785T
Other languages
English (en)
Other versions
DE69029785T2 (de
Inventor
Ronald N Berzofsky
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lonza Walkersville Inc
Original Assignee
BioWhittaker Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23698313&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69029785(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by BioWhittaker Inc filed Critical BioWhittaker Inc
Publication of DE69029785D1 publication Critical patent/DE69029785D1/de
Application granted granted Critical
Publication of DE69029785T2 publication Critical patent/DE69029785T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/579—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving limulus lysate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
    • C12Q2337/10—Anilides
    • C12Q2337/12—Para-Nitroanilides p-NA
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
DE69029785T 1989-10-30 1990-10-26 Kinetische Bestimmungsmethode für Endotoxin unter Verwendung von Limulus-Amoebocyten-Lysat und chromogenem Substrat Expired - Lifetime DE69029785T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US42830089A 1989-10-30 1989-10-30

Publications (2)

Publication Number Publication Date
DE69029785D1 true DE69029785D1 (de) 1997-03-06
DE69029785T2 DE69029785T2 (de) 1997-05-28

Family

ID=23698313

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69029785T Expired - Lifetime DE69029785T2 (de) 1989-10-30 1990-10-26 Kinetische Bestimmungsmethode für Endotoxin unter Verwendung von Limulus-Amoebocyten-Lysat und chromogenem Substrat

Country Status (10)

Country Link
US (1) US5310657A (de)
EP (1) EP0426395B1 (de)
JP (1) JP2955345B2 (de)
AT (1) ATE148230T1 (de)
AU (1) AU644109B2 (de)
CA (1) CA2028789C (de)
DE (1) DE69029785T2 (de)
DK (1) DK0426395T3 (de)
ES (1) ES2098256T3 (de)
GR (1) GR3023298T3 (de)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69211973T2 (de) * 1991-12-25 1996-12-19 Maruha Corp Reagenzien und Verfahren zur Bestimmung von Beta-Glucanen
JP3242733B2 (ja) * 1993-02-26 2001-12-25 生化学工業株式会社 エンドトキシン特異的測定剤
US6270982B1 (en) 1997-10-09 2001-08-07 Charles River Laboratories Methods and compositions for the detection of bacterial endotoxins
JP4235279B2 (ja) * 1998-04-10 2009-03-11 生化学工業株式会社 新規酵素反応測定法
BR0309703A (pt) * 2002-04-30 2005-02-09 Biowhittaker Technologies Inc Sistemas de análise de injeção sequencial automatizada para a determinação de nìveis de traços de endotoxina
US7547285B2 (en) * 2003-02-14 2009-06-16 The Charlotte-Mecklenburg Hospital Authority Device and method for collection of exhaled alveolar breath condensate
US20040138577A1 (en) * 2002-12-20 2004-07-15 Kline Jeffrey A. Disposable hand-held device for collection of exhaled breath condensate
US7828741B2 (en) * 2002-12-20 2010-11-09 The Charlotte-Mecklenburg Hospital Authority Utilizing lipopolysaccharide in exhaled breath condensate to diagnose gram negative pneumonia
DE602004024769D1 (de) 2003-03-17 2010-02-04 Charles River Lab Inc Methoden und zusammensetzungen für den nachweis mikrobieller verunreinigungen
US20050048655A1 (en) * 2003-04-18 2005-03-03 Novitsky Thomas J. Kit for detecting endotoxin
EP1627040A2 (de) * 2003-04-18 2006-02-22 Associates Of Cape Cod, Inc. Kit zum nachweis von endotoxin in wässrigen lösungen
JP2007510911A (ja) * 2003-11-07 2007-04-26 キャンブレックス・バイオ・サイエンス・ウォーカーズヴィル・インコーポレーテッド 内毒素レベルを測定するオンライン装置および方法
EP1842069A2 (de) * 2004-12-02 2007-10-10 Charles River Laboratories, Inc. Verfahren und zusammensetzungen zum nachweis und/oder zur quantifizierung grampositiver bakterienkontaminanten
ATE419526T1 (de) 2005-01-13 2009-01-15 Charles River Lab Inc Verfahren zur klassifizierung von mikroben in einer biologischen probe
EP2050820A4 (de) * 2006-07-07 2009-12-16 Seikagaku Kogyo Co Ltd Progerinnungsenzym und verfahren zum nachweis von endotoxin oder (1 3)- -d-glucan unter verwendung davon
US20080124248A1 (en) * 2006-11-29 2008-05-29 Edward Andrew Sobek Endotoxins and glucans quantification in indoor air
US20090286692A1 (en) * 2008-04-15 2009-11-19 Wainwright Norman R Cartridge and Method for Sample Analysis
JP5489680B2 (ja) * 2009-12-02 2014-05-14 興和株式会社 生物由来の生理活性物質の測定方法、それを実行するためのプログラム及び、生物由来の生理活性物質の測定装置
EP2407788A4 (de) * 2009-03-13 2013-04-17 Kowa Co Verfahren zur messung von biologisch aktiven biogenen stoffen, programm zur umsetzung des verfahrens sowie vorrichtung zur messung von biologisch aktiven biogenen stoffen
US10473663B2 (en) 2009-06-26 2019-11-12 Charles River Laboratories, Inc. Heat-treated limulus amebocyte lysates
GB2477752A (en) * 2010-02-11 2011-08-17 Arab Biotechnology Company Detection of bacteria
CU24073B1 (es) 2011-12-27 2015-01-29 Ct De Investigación Y Desarrollo De Medicamentos Cidem COMPOSICIÓN A PARTIR DE EXTRACTO DE HEMOCITOS DE LANGOSTA PARA LA DETECCIÓN DE LIPOPOLISACÁRIDOS, PEPTIDOGLICANOS Y 1,3-ß-D-GLUCANOS
US20130078665A1 (en) * 2012-07-18 2013-03-28 Deepika Bodapati Test strip, a test kit and a method for detection of endotoxin in food
EP2904399B8 (de) * 2012-10-08 2019-03-13 BL Technologies, Inc. Mikrofluidische zentripetale plattform zum testen von lal-reaktiven substanzen
US10309947B2 (en) 2013-02-27 2019-06-04 Trace-Ability, Inc. System and method for radiosynthesis, quality control and dose dispensing
US11002717B2 (en) 2014-02-26 2021-05-11 Trace-Ability, Inc. Systems and methods for characterizing radioactive analytes
US10895563B2 (en) 2014-02-26 2021-01-19 Trace-Ability, Inc. Palette-based systems for analyte characterization
KR102561368B1 (ko) * 2016-08-03 2023-08-01 론자 워커스빌 아이엔씨. 실질적으로 코아굴로겐이 없는 리물루스 아메보사이트 라이세이트를 이용한 내독소 검출방법
CN110192110B (zh) * 2017-01-11 2023-01-10 隆萨沃克斯维尔股份有限公司 无凝固蛋白原的澄清的鲎阿米巴样细胞裂解物
US20190001330A1 (en) 2017-06-28 2019-01-03 Lonza Walkersville, Inc. Cartridge for endotoxin detection
US11892397B2 (en) * 2017-10-17 2024-02-06 Board Of Regents, The University Of Texas System Endotoxin testing assay and method of same
CN109387637A (zh) * 2018-10-12 2019-02-26 四川升和药业股份有限公司 丹参注射液细菌内毒素检测方法
CN111077307B (zh) * 2018-10-21 2022-08-23 厦门鲎试剂生物科技股份有限公司 一种用革兰氏阴性细菌感染快速检测脓毒病的新方法
US12158471B2 (en) 2022-02-11 2024-12-03 Charles River Laboratories, Inc. Recombinant amebocyte lysate and uses thereof

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5415797A (en) * 1977-06-14 1979-02-05 Seikagaku Kogyo Co Ltd Detection and measurement of toxin in cells
US4322217A (en) * 1980-06-27 1982-03-30 Mallinckrodt, Inc. Process for preparing Limulus lysate
US4406832A (en) * 1981-09-03 1983-09-27 Mallinckrodt, Inc. Peptide-type substrates useful in the quantitative determination of endotoxin
US4510241A (en) * 1981-09-03 1985-04-09 Mallinckrodt, Inc. Peptide-type substrates useful in the quantitative determination of endotoxin
DE3439761A1 (de) * 1984-10-31 1986-05-07 Klaus-Peter 6800 Mannheim Becker Verfahren zur bestimmung von endotoxinkonzentrationen

Also Published As

Publication number Publication date
AU644109B2 (en) 1993-12-02
JPH03220456A (ja) 1991-09-27
CA2028789A1 (en) 1991-05-01
DK0426395T3 (de) 1997-03-03
AU6555690A (en) 1991-05-02
GR3023298T3 (en) 1997-07-30
JP2955345B2 (ja) 1999-10-04
ATE148230T1 (de) 1997-02-15
EP0426395B1 (de) 1997-01-22
US5310657A (en) 1994-05-10
EP0426395A1 (de) 1991-05-08
ES2098256T3 (es) 1997-05-01
DE69029785T2 (de) 1997-05-28
CA2028789C (en) 2001-02-06

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