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DE60334109D1 - Yapsin-mangelmutantenstamm von hansenula polymorpha sowie verfahren zur herstellung rekombinanter proteine damit - Google Patents

Yapsin-mangelmutantenstamm von hansenula polymorpha sowie verfahren zur herstellung rekombinanter proteine damit

Info

Publication number
DE60334109D1
DE60334109D1 DE60334109T DE60334109T DE60334109D1 DE 60334109 D1 DE60334109 D1 DE 60334109D1 DE 60334109 T DE60334109 T DE 60334109T DE 60334109 T DE60334109 T DE 60334109T DE 60334109 D1 DE60334109 D1 DE 60334109D1
Authority
DE
Germany
Prior art keywords
polymorpha
foreign protein
yapsin
lack
production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60334109T
Other languages
English (en)
Inventor
Hyun-Ah Kang
Sang-Ki Rhee
Min-Jeong Sohn
Jeong-Yoon Kim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Korea Research Institute of Bioscience and Biotechnology KRIBB
Leadbio Inc
Bioholdings Co Ltd
Original Assignee
Korea Research Institute of Bioscience and Biotechnology KRIBB
Leadbio Inc
Bioholdings Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Korea Research Institute of Bioscience and Biotechnology KRIBB, Leadbio Inc, Bioholdings Co Ltd filed Critical Korea Research Institute of Bioscience and Biotechnology KRIBB
Application granted granted Critical
Publication of DE60334109D1 publication Critical patent/DE60334109D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
DE60334109T 2002-06-29 2003-06-28 Yapsin-mangelmutantenstamm von hansenula polymorpha sowie verfahren zur herstellung rekombinanter proteine damit Expired - Lifetime DE60334109D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20020037718 2002-06-29
PCT/KR2003/001279 WO2004003204A1 (en) 2002-06-29 2003-06-28 Hansenula polymorpha yapsin deficient mutant strain and process for the preparation of recombinant proteins using the same

Publications (1)

Publication Number Publication Date
DE60334109D1 true DE60334109D1 (de) 2010-10-21

Family

ID=29997410

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60334109T Expired - Lifetime DE60334109D1 (de) 2002-06-29 2003-06-28 Yapsin-mangelmutantenstamm von hansenula polymorpha sowie verfahren zur herstellung rekombinanter proteine damit

Country Status (7)

Country Link
US (1) US7262287B2 (de)
EP (1) EP1534835B1 (de)
KR (1) KR100496758B1 (de)
AT (1) ATE480624T1 (de)
AU (1) AU2003237061A1 (de)
DE (1) DE60334109D1 (de)
WO (1) WO2004003204A1 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004092408A2 (en) * 2003-04-18 2004-10-28 Warnex Research Inc. Oligonucleotides for the detection of salmonella species
KR100977446B1 (ko) * 2006-06-16 2010-08-24 한국생명공학연구원 분비 스트레스 반응을 조절하는 한세눌라 폴리모르파의신규한 유전자 및 상기 유전자를 이용하여 재조합 단백질의분비 발현 효율을 증가시키는 방법
CA3069431A1 (en) 2007-04-03 2008-10-09 Oxyrane Uk Limited Glycosylation of molecules
US9120871B2 (en) * 2009-01-23 2015-09-01 Novo Nordisk A/S Process for preparing FGF21 with low degree of O-glycosylation
CN104651428A (zh) 2009-09-29 2015-05-27 根特大学 水解甘露糖-1-磷酸-6-甘露糖连接为磷酸-6-甘露糖
US20130053550A1 (en) 2009-11-19 2013-02-28 Oxyrane Uk Limited Yeast strains producing mammalian-like complex n-glycans
EP2504355B1 (de) * 2009-11-25 2015-07-22 Novo Nordisk A/S Verfahren zur herstellung von polypeptiden
BR112013007263A2 (pt) 2010-09-29 2016-06-14 Oxyrane Uk Ltd desmanosilação de n-glicanos fosforilados
EP2622088A2 (de) 2010-09-29 2013-08-07 Oxyrane UK Limited Mannosidasen zum entkappen von mannose-1-phospho-6-mannose-bindungen und demannosylierung von phosphorylierten n-glykanen sowie verfahren zur ermöglichung der zellulären aufnahme von glykoproteinen durch säugerzellen
RU2644258C2 (ru) 2012-03-15 2018-02-08 Оксирейн Юкей Лимитед Способы и материалы для лечения болезни помпе
US10647975B2 (en) 2017-10-03 2020-05-12 Bolt Threads, Inc. Modified strains for the production of recombinant silk
CN111315763A (zh) * 2017-10-03 2020-06-19 保尔特纺织品公司 用于生产重组丝的经修饰菌株

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0336056B1 (de) * 1988-01-27 1995-04-05 M & D RESEARCH CO., LTD. Protease
US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
JPH07108232B2 (ja) * 1990-10-09 1995-11-22 エム・ディ・リサーチ株式会社 ペプチド又は蛋白質の製造方法
DK82893D0 (da) * 1993-07-08 1993-07-08 Novo Nordisk As Peptid
GB9404270D0 (en) 1994-03-05 1994-04-20 Delta Biotechnology Ltd Yeast strains and modified albumins
DE69717629T2 (de) * 1996-07-05 2003-09-11 Novo Nordisk A/S, Bagsvaerd Verfahren zur produktion von polypeptiden
US6110703A (en) 1996-07-05 2000-08-29 Novo Nordisk A/S Method for the production of polypeptides
KR100246932B1 (ko) * 1997-09-12 2000-03-15 박호군 Yap3 유전자가 결손된 신규한 효모균주 및 그를 이용한 재조합 인체 부갑상선호르몬의 생산
WO2000052133A2 (en) 1999-03-04 2000-09-08 Korea Research Institute Of Bioscience And Biotechnology Hansenula polymorpha mutants and process for the preparation of recombinant proteins using the same
KR100441384B1 (ko) 2002-01-08 2004-07-21 주식회사 안지오랩 인간 혈청 알부민-팀프-2 융합 단백질 발현시스템 및재조합 인간 혈청 알부민-팀프-2 융합 단백질
KR102008884B1 (ko) 2014-01-23 2019-08-09 동우 화인켐 주식회사 실리콘계 화합물막 식각액 조성물
KR102001775B1 (ko) 2018-04-10 2019-07-18 조성은 Led 렌즈 및 이를 포함한 led 패키지

Also Published As

Publication number Publication date
AU2003237061A8 (en) 2004-01-19
WO2004003204A1 (en) 2004-01-08
KR20040002806A (ko) 2004-01-07
EP1534835A1 (de) 2005-06-01
AU2003237061A1 (en) 2004-01-19
KR100496758B1 (ko) 2005-06-22
US7262287B2 (en) 2007-08-28
US20050239074A1 (en) 2005-10-27
EP1534835A4 (de) 2005-12-14
EP1534835B1 (de) 2010-09-08
ATE480624T1 (de) 2010-09-15

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