DE60043889D1 - Tet repressor basierte transkriptionale regulatorische proteine - Google Patents
Tet repressor basierte transkriptionale regulatorische proteineInfo
- Publication number
- DE60043889D1 DE60043889D1 DE60043889T DE60043889T DE60043889D1 DE 60043889 D1 DE60043889 D1 DE 60043889D1 DE 60043889 T DE60043889 T DE 60043889T DE 60043889 T DE60043889 T DE 60043889T DE 60043889 D1 DE60043889 D1 DE 60043889D1
- Authority
- DE
- Germany
- Prior art keywords
- tetracycline
- transkriptionale
- regulatorische
- basierte
- proteine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/108—Plasmid DNA episomal vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
- C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
- C12N2830/003—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor tet inducible
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
- C12N2830/005—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB
- C12N2830/006—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB tet repressible
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Communicable Diseases (AREA)
- Virology (AREA)
- Oncology (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US13798699P | 1999-06-07 | 1999-06-07 | |
PCT/IB2000/000886 WO2000075347A2 (en) | 1999-06-07 | 2000-06-05 | Tet repressor-based transcriptional regulatory proteins |
Publications (1)
Publication Number | Publication Date |
---|---|
DE60043889D1 true DE60043889D1 (en) | 2010-04-08 |
Family
ID=22479925
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE60043889T Expired - Fee Related DE60043889D1 (en) | 1999-06-07 | 2000-06-05 | Tet repressor basierte transkriptionale regulatorische proteine |
Country Status (8)
Country | Link |
---|---|
US (1) | US7541446B2 (de) |
EP (1) | EP1200607B1 (de) |
JP (1) | JP4836375B2 (de) |
AT (1) | ATE458818T1 (de) |
AU (1) | AU783233B2 (de) |
CA (1) | CA2376665C (de) |
DE (1) | DE60043889D1 (de) |
WO (1) | WO2000075347A2 (de) |
Families Citing this family (37)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20030186281A1 (en) * | 2001-12-21 | 2003-10-02 | Wolfgang Hillen | Modified tetracycline repressor protein compositions and methods of use |
US8507277B2 (en) | 2003-10-24 | 2013-08-13 | Gencia Corporation | Nonviral vectors for delivering polynucleotides |
US20090123468A1 (en) | 2003-10-24 | 2009-05-14 | Gencia Corporation | Transducible polypeptides for modifying metabolism |
US8062891B2 (en) | 2003-10-24 | 2011-11-22 | Gencia Corporation | Nonviral vectors for delivering polynucleotides to plants |
US8133733B2 (en) | 2003-10-24 | 2012-03-13 | Gencia Corporation | Nonviral vectors for delivering polynucleotides to target tissues |
US8039587B2 (en) | 2003-10-24 | 2011-10-18 | Gencia Corporation | Methods and compositions for delivering polynucleotides |
WO2006037215A1 (en) * | 2004-10-04 | 2006-04-13 | National Research Council Of Canada | Expression system, components thereof and methods of use |
US7935788B2 (en) * | 2004-10-04 | 2011-05-03 | National Research Council Of Canada | Reverse cumate repressor mutant |
US9068969B2 (en) * | 2004-12-28 | 2015-06-30 | Ptc Therapeutics, Inc. | Cell based methods and systems for the identification of RNA regulatory sequences and compounds that modulate their functions |
JP4944781B2 (ja) | 2005-09-15 | 2012-06-06 | 独立行政法人科学技術振興機構 | テトラサイクリン系抗生物質によるタンパク質分解制御法 |
USRE49583E1 (en) | 2005-11-17 | 2023-07-18 | Tet Systems Gmbh & Co. Kg | Inducible expression systems |
JP2009537139A (ja) * | 2006-05-15 | 2009-10-29 | パラテック ファーマシューティカルズ インコーポレイテッド | 置換されたテトラサイクリン化合物を用いて、遺伝子または遺伝子産物の発現を制御する方法 |
EP2043662B1 (de) * | 2006-07-26 | 2015-09-09 | Intrexon Corporation | Verfahren und zusammensetzungen zur behandlung von krankheiten |
EP2135945B1 (de) | 2007-03-14 | 2014-04-23 | Japan Science and Technology Agency | Verfahren zur doppelten regulation von transkription/abbau für ein protein mittels antibiotikum |
US20080235746A1 (en) | 2007-03-20 | 2008-09-25 | Michael James Peters | Methods and apparatus for content delivery and replacement in a network |
WO2010037593A1 (en) | 2008-10-01 | 2010-04-08 | Tet Systems Holding Gmbh & Co.Kg | Tetracycline inducible transcription control sequence |
US8257956B2 (en) | 2008-10-28 | 2012-09-04 | E. I. Du Pont De Nemours And Company | Sulfonylurea-responsive repressor proteins |
US9866609B2 (en) | 2009-06-08 | 2018-01-09 | Time Warner Cable Enterprises Llc | Methods and apparatus for premises content distribution |
MX2012012211A (es) * | 2010-04-23 | 2012-12-17 | Du Pont | Composiciones de interruptores genicos y metodos de uso de las mismas. |
EP2643459B1 (de) * | 2010-11-24 | 2017-09-27 | Takara Bio USA, Inc. | Transkriptionsmodulatoren mit einem system für induzierbare expression und einer domäne für verteilte proteintransduktion |
JP2014506458A (ja) * | 2011-01-26 | 2014-03-17 | クロンテック・ラボラトリーズ・インコーポレーテッド | タンパク質形質導入の向上 |
US9822379B2 (en) | 2011-05-19 | 2017-11-21 | Fundación Pública Andaluza Progreso Y Salud | Highly inducible dual-promoter lentiviral TET-ON system |
SG10201606970UA (en) | 2012-02-23 | 2016-10-28 | Juno Therapeutics Gmbh | Chromatographic isolation of cells and other complex biological materials |
US20180245038A1 (en) | 2015-09-04 | 2018-08-30 | Universita' Degli Studi Di Padova | Method for generating somatic stem cells |
CN108474002B (zh) | 2015-10-22 | 2023-05-23 | 朱诺治疗学有限公司 | 用于转导的方法、反应剂盒、反应剂和设备 |
MA45489A (fr) | 2015-10-22 | 2018-08-29 | Juno Therapeutics Gmbh | Procédés de culture de cellules, kits et appareil associés |
WO2017205852A2 (en) | 2016-05-27 | 2017-11-30 | Synthex, Inc. | Protein interfaces |
US10687115B2 (en) | 2016-06-01 | 2020-06-16 | Time Warner Cable Enterprises Llc | Cloud-based digital content recorder apparatus and methods |
US10129593B2 (en) | 2017-03-14 | 2018-11-13 | Charter Communications Operating, Llc | Time-based dynamic secondary content placement calls in time-shifted content |
SG11201909931PA (en) | 2017-04-27 | 2019-11-28 | Juno Therapeutics Gmbh | Oligomeric particle reagents and methods of use thereof |
GB2584210B (en) | 2017-11-22 | 2022-03-16 | Synthex Inc | Peptides for inhibiting RAD51 |
US10939142B2 (en) | 2018-02-27 | 2021-03-02 | Charter Communications Operating, Llc | Apparatus and methods for content storage, distribution and security within a content distribution network |
CN113584083A (zh) | 2020-04-30 | 2021-11-02 | 深圳市深研生物科技有限公司 | 用于逆转录病毒载体的生产细胞和包装细胞及其制备方法 |
CN113699147B (zh) | 2020-05-22 | 2023-06-09 | 深圳市深研生物科技有限公司 | 基于四环素和Cumate的共调控序列 |
US11566262B2 (en) | 2021-03-24 | 2023-01-31 | Dna Twopointo Inc. | Tetracycline-inducible expression systems |
CN114134160B (zh) * | 2021-12-04 | 2024-01-09 | 安徽大学 | 一种四环素调控蛋白突变体基因及其在调控基因表达和环境检测中的应用 |
US20240228561A1 (en) | 2023-01-06 | 2024-07-11 | Life Biosciences, Inc. | Methods Of Treating Non-Arteritic Anterior Ischemic Optic Neuropathy |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1994004672A1 (en) * | 1992-08-26 | 1994-03-03 | Dnx Corp. | Tetracycline repressor-mediated binary regulation system for control of gene expression in transgenic animals |
US5464758A (en) * | 1993-06-14 | 1995-11-07 | Gossen; Manfred | Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters |
US5589362A (en) * | 1993-06-14 | 1996-12-31 | Basf Aktiengesellschaft | Tetracycline regulated transcriptional modulators with altered DNA binding specificities |
US5650298A (en) * | 1993-06-14 | 1997-07-22 | Basf Aktiengesellschaft | Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters |
US5866755A (en) * | 1993-06-14 | 1999-02-02 | Basf Aktiengellschaft | Animals transgenic for a tetracycline-regulated transcriptional inhibitor |
US6004941A (en) * | 1993-06-14 | 1999-12-21 | Basf Aktiengesellschaft | Methods for regulating gene expression |
DE4417598A1 (de) * | 1994-05-19 | 1995-12-14 | Max Planck Gesellschaft | Verwendung des Tetracyclinpromotors zur stringent regulierten Produktion von rekombinanten Proteinen in prokaryontischen Zellen |
US5851796A (en) * | 1995-06-07 | 1998-12-22 | Yale University | Autoregulatory tetracycline-regulated system for inducible gene expression in eucaryotes |
US6114148C1 (en) * | 1996-09-20 | 2012-05-01 | Gen Hospital Corp | High level expression of proteins |
US6087166A (en) * | 1997-07-03 | 2000-07-11 | Basf Aktiengesellschaft | Transcriptional activators with graded transactivation potential |
DE19851415B4 (de) | 1998-11-07 | 2010-11-04 | Wolfgang Hillen | Mutanter Transaktivator |
DE19851413C2 (de) | 1998-11-07 | 2003-05-22 | Wolfgang Hillen | Verfahren zur Auswahl von Mutanten von tTA oder rtTA |
-
2000
- 2000-06-05 JP JP2001501629A patent/JP4836375B2/ja not_active Expired - Lifetime
- 2000-06-05 AU AU54191/00A patent/AU783233B2/en not_active Expired
- 2000-06-05 WO PCT/IB2000/000886 patent/WO2000075347A2/en active IP Right Grant
- 2000-06-05 DE DE60043889T patent/DE60043889D1/de not_active Expired - Fee Related
- 2000-06-05 EP EP00938973A patent/EP1200607B1/de not_active Expired - Lifetime
- 2000-06-05 CA CA2376665A patent/CA2376665C/en not_active Expired - Lifetime
- 2000-06-05 AT AT00938973T patent/ATE458818T1/de not_active IP Right Cessation
-
2003
- 2003-06-05 US US10/456,395 patent/US7541446B2/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
JP4836375B2 (ja) | 2011-12-14 |
AU5419100A (en) | 2000-12-28 |
WO2000075347A2 (en) | 2000-12-14 |
US20030208783A1 (en) | 2003-11-06 |
EP1200607A2 (de) | 2002-05-02 |
JP2003515314A (ja) | 2003-05-07 |
ATE458818T1 (de) | 2010-03-15 |
AU783233B2 (en) | 2005-10-06 |
EP1200607B1 (de) | 2010-02-24 |
CA2376665C (en) | 2014-02-25 |
CA2376665A1 (en) | 2000-12-14 |
US7541446B2 (en) | 2009-06-02 |
WO2000075347A3 (en) | 2002-01-10 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition | ||
8339 | Ceased/non-payment of the annual fee |