CN2476023Y - Hepatitis B surface antigen and a-fetoprotein double linking colloidal gold immune chromatography test paper slip - Google Patents
Hepatitis B surface antigen and a-fetoprotein double linking colloidal gold immune chromatography test paper slip Download PDFInfo
- Publication number
- CN2476023Y CN2476023Y CN 01239923 CN01239923U CN2476023Y CN 2476023 Y CN2476023 Y CN 2476023Y CN 01239923 CN01239923 CN 01239923 CN 01239923 U CN01239923 U CN 01239923U CN 2476023 Y CN2476023 Y CN 2476023Y
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- China
- Prior art keywords
- hepatitis
- afp
- hbsag
- fetoprotein
- surface antigen
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- Expired - Fee Related
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- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
The utility model is a hepatitis b surface antigen and Alpha-fetoprotein duplex gold immunochromatographic assay test paper for the detection of hepatitis b surface antigen and Alpha-fetoprotein. The structure thereof is that the two sides of the cellulose membrane above the vector are provided with a colloidal gold conjugate pad and an absorption pad respectively. The membrane between the two pads is provided successively with a control line, an AFP test line and an HBsAg test line and a sample pad is provided above the HBsAg plus AFP colloidal gold conjugate pad. The utility model has advantages of simple and novel structure, easy and speed use; the AFP and HBsAg indexes can be acquired simultaneously through a single test. Thus, the model utility has a broad popularization and application prospect.
Description
The utility model relates to a kind of hepatitis B surface antigen and alpha-fetoprotein duplex colloidal gold immuno-chromatography test paper strip that hepatitis B surface antigen and alpha-fetoprotein are used that detect.
Detecting hepatitis B surface antigen (HBsAg) and alpha-fetoprotein (AFP) is two important lab index of diagnosis of hepatitis b virus infections and auxiliary diagnosis primary carcinoma of liver.Human hepatitis B virus infects with primary carcinoma of liver and has very strong correlativity, and whether the secondary primary carcinoma of liver is helpful to judging the chronic hepatitis patient and detect AFP.Therefore clinically, the patient of or primary carcinoma of liver hepatitis b virus infected for many trouble under a cloud usually needs to measure simultaneously its HBsAg and AFP.In China, the mass survey of liver cancer is all measured HBsAg and two indexs of AFP simultaneously, yet, at present, detect HBsAg, AFP both at home and abroad and generally adopt enzyme immunoadsorption (ELISA) kit to detect human serum HBsAg, AFP.This kit running program complexity, influence factor is many, and experimental period is longer, is subjected to condition restriction, needs to be equipped with certain instrument and equipment, easily causes the operator to infect and environmental pollution, brings inconvenience to experiment.And detect HBsAg and AFP or separated measuring, both time-consuming, inconvenient again.Though the market has the single test strips of HBsAg, AFP, when using, need test respectively, still inconvenient.
The purpose of this utility model provides a kind of novel hepatitis B surface antigen and alpha-fetoprotein duplex colloidal gold immuno-chromatography test paper strip, can solve the problem that one-time detection can obtain hepatitis B surface antigen and alpha-fetoprotein simultaneously effectively.
The purpose of this utility model is achieved like this, on the nitrocellulose filter (being N, C film) on the carrier of making by PVC (PVC plate) top, be fixed with nature controlling line (polyclone body), AFP detection line, HBsAg detection line respectively, one end of film has collaurum bond pad, the sample pad (glass fibre) that contains dry red glue gold mark monoclonal antibody, and the other end has absorption pad (cotton starch plate).
The utility model novel structure, simple, one-time detection can obtain two results of HBsAg and AFP simultaneously, has special, responsive, simple, quick, economic dispatch characteristics, in clinical and crowd's general investigation of desease application value is widely arranged.
Below in conjunction with accompanying drawing structure of the present utility model and operating position are elaborated.
Accompanying drawing is the utility model structural front view (enlarged diagram).
Provide by figure, the utility model is at nitrocellulose filter 2 two ends on carrier 1 top HBsAg+AFP collaurum bond pad 8 and absorption pad 3 to be arranged respectively, on nitrocellulose filter 2, be fixed with nature controlling line 4, AFP detection line 5, HBsAg detection line 6 according to this in the middle of two pads, sample pad 7 arranged on HBsAg+AFP collaurum bond pad 8 tops.
This test strips operating position be because of with monoclonal antibody anti--HBsAg and anti--AFP detection line and polyclonal antibody nature controlling line be separately fixed at N, on the C film (nitrocellulose filter), end at film sticks the collaurum bond pad that contains dry red colloid gold label monoclonal antibody, and serum specimen dripped on an end sample pad of film, utilize N, the capillarity of C film, the absorption pad of being made by cotton starch plate to the other end moves, just as chromatography, serum specimen is travelling to when being fixed with antibody regional, corresponding antigen promptly combines with this antibody generation specificity in the sample, produce macroscopic red stripes, the colour developing depth is directly proportional with antigenic content, thereby reach the purpose that records HBsAg and AFP simultaneously, fast, convenient and accurate, it is applied to cure the disease for mankind's epidemic prevention and make creative contribution.
Claims (1)
1, a kind of hepatitis B surface antigen and alpha-fetoprotein duplex colloidal gold immuno-chromatography test paper strip, nitrocellulose filter (2) two ends that it is characterized in that (1) top on carrier have HBsAg+AFP collaurum bond pad (8) and absorption pad (3) respectively, on nitrocellulose filter (2), be fixed with nature controlling line (4), AFP detection line (5), HBsAg detection line (6) according to this in the middle of two pads, sample pad (7) is arranged on HBsAg+AFP collaurum bond pad (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01239923 CN2476023Y (en) | 2001-04-29 | 2001-04-29 | Hepatitis B surface antigen and a-fetoprotein double linking colloidal gold immune chromatography test paper slip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01239923 CN2476023Y (en) | 2001-04-29 | 2001-04-29 | Hepatitis B surface antigen and a-fetoprotein double linking colloidal gold immune chromatography test paper slip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2476023Y true CN2476023Y (en) | 2002-02-06 |
Family
ID=33652258
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 01239923 Expired - Fee Related CN2476023Y (en) | 2001-04-29 | 2001-04-29 | Hepatitis B surface antigen and a-fetoprotein double linking colloidal gold immune chromatography test paper slip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2476023Y (en) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1311239C (en) * | 2004-09-07 | 2007-04-18 | 李人 | Immune chromatograph testing strip and production thereof |
| WO2008014709A1 (en) * | 2006-07-26 | 2008-02-07 | Abon Biopharm (Hangzhou) Co., Ltd. | Analysis device for biologicla sample |
| CN1831536B (en) * | 2005-03-08 | 2010-05-05 | 中国人民解放军军事医学科学院微生物流行病研究所 | Immunization chromatograph test paper for detecting plague yersinia and preparation method thereof |
| US7837939B2 (en) | 2003-11-14 | 2010-11-23 | Alere Switzerland Gmbh | Rapid sample collection and analysis device and methods of use |
| CN101915842A (en) * | 2010-07-26 | 2010-12-15 | 河南省科学院生物研究所有限责任公司 | Binary detection test strip of beta-exhilarant ractopamine and salbutamol and preparation method thereof |
| CN102323425A (en) * | 2011-06-03 | 2012-01-18 | 正元盛邦(天津)生物科技有限公司 | Method for semi-quantitatively diagnosing alpha-fetoprotein by using double-indicatrix immunochromatography |
| CN101566636B (en) * | 2008-04-24 | 2012-10-24 | 北京科美生物技术有限公司 | Preparation method of magnetic immunochromatographic test strip for quantitatively detecting alpha-fetoprotein in blood |
| US8871155B2 (en) | 2005-11-30 | 2014-10-28 | Alere Switzerland Gmbh | Devices for detecting analytes in fluid sample |
| CN104730237A (en) * | 2013-12-23 | 2015-06-24 | 国家纳米科学中心 | Test paper, application and method for alpha-fetoprotein antigen, hepatitis B surface antigen or HIV gp41 antibody |
| CN105807070A (en) * | 2016-06-08 | 2016-07-27 | 广西中医药大学附属瑞康医院 | Kit for detecting hepatitis B virus |
| CN106405091A (en) * | 2016-08-28 | 2017-02-15 | 李献兵 | Mediastinal malignant teratoma detecction colloidal gold immunochromatographic test paper and preparation method thereof |
-
2001
- 2001-04-29 CN CN 01239923 patent/CN2476023Y/en not_active Expired - Fee Related
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7837939B2 (en) | 2003-11-14 | 2010-11-23 | Alere Switzerland Gmbh | Rapid sample collection and analysis device and methods of use |
| CN1311239C (en) * | 2004-09-07 | 2007-04-18 | 李人 | Immune chromatograph testing strip and production thereof |
| CN1831536B (en) * | 2005-03-08 | 2010-05-05 | 中国人民解放军军事医学科学院微生物流行病研究所 | Immunization chromatograph test paper for detecting plague yersinia and preparation method thereof |
| US8871155B2 (en) | 2005-11-30 | 2014-10-28 | Alere Switzerland Gmbh | Devices for detecting analytes in fluid sample |
| US8071394B2 (en) | 2006-07-26 | 2011-12-06 | Alere Switzerland Gmbh | Test device for detecting an analyte in a liquid sample |
| WO2008014709A1 (en) * | 2006-07-26 | 2008-02-07 | Abon Biopharm (Hangzhou) Co., Ltd. | Analysis device for biologicla sample |
| CN101566636B (en) * | 2008-04-24 | 2012-10-24 | 北京科美生物技术有限公司 | Preparation method of magnetic immunochromatographic test strip for quantitatively detecting alpha-fetoprotein in blood |
| CN101915842A (en) * | 2010-07-26 | 2010-12-15 | 河南省科学院生物研究所有限责任公司 | Binary detection test strip of beta-exhilarant ractopamine and salbutamol and preparation method thereof |
| CN101915842B (en) * | 2010-07-26 | 2013-04-03 | 河南省科学院生物研究所有限责任公司 | Binary detection test strip of beta-exhilarant ractopamine and salbutamol and preparation method thereof |
| CN102323425A (en) * | 2011-06-03 | 2012-01-18 | 正元盛邦(天津)生物科技有限公司 | Method for semi-quantitatively diagnosing alpha-fetoprotein by using double-indicatrix immunochromatography |
| CN104730237A (en) * | 2013-12-23 | 2015-06-24 | 国家纳米科学中心 | Test paper, application and method for alpha-fetoprotein antigen, hepatitis B surface antigen or HIV gp41 antibody |
| CN105807070A (en) * | 2016-06-08 | 2016-07-27 | 广西中医药大学附属瑞康医院 | Kit for detecting hepatitis B virus |
| CN106405091A (en) * | 2016-08-28 | 2017-02-15 | 李献兵 | Mediastinal malignant teratoma detecction colloidal gold immunochromatographic test paper and preparation method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |