CN221572418U - Protein electrophoresis gel film transferring device suitable for underwater operation - Google Patents
Protein electrophoresis gel film transferring device suitable for underwater operation Download PDFInfo
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- CN221572418U CN221572418U CN202322915046.3U CN202322915046U CN221572418U CN 221572418 U CN221572418 U CN 221572418U CN 202322915046 U CN202322915046 U CN 202322915046U CN 221572418 U CN221572418 U CN 221572418U
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- liquid storage
- cooling
- storage tank
- liquid
- conductive columns
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- 238000001962 electrophoresis Methods 0.000 title claims abstract description 39
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 23
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 23
- 108010025899 gelatin film Proteins 0.000 title abstract description 5
- 239000007788 liquid Substances 0.000 claims abstract description 80
- 238000001816 cooling Methods 0.000 claims abstract description 38
- 238000012546 transfer Methods 0.000 claims description 32
- 238000003825 pressing Methods 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 239000000110 cooling liquid Substances 0.000 claims description 5
- 238000007789 sealing Methods 0.000 claims description 5
- 238000002347 injection Methods 0.000 claims description 4
- 239000007924 injection Substances 0.000 claims description 4
- 239000002033 PVDF binder Substances 0.000 abstract description 29
- 229920002981 polyvinylidene fluoride Polymers 0.000 abstract description 29
- 239000012528 membrane Substances 0.000 abstract description 26
- 238000000034 method Methods 0.000 abstract description 12
- 230000000694 effects Effects 0.000 abstract description 4
- 238000002474 experimental method Methods 0.000 description 3
- 238000013519 translation Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 2
- 238000003119 immunoblot Methods 0.000 description 2
- 230000003044 adaptive effect Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
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- Peptides Or Proteins (AREA)
Abstract
The utility model discloses a protein electrophoresis gel film transferring device suitable for underwater operation, which comprises a liquid storage tank and a tank cover, wherein a pair of conductive columns are arranged in the liquid storage tank at intervals, the upper ends of the conductive columns vertically extend upwards to penetrate out of the tank cover, a plurality of slots are formed in the bottom surface of the liquid storage tank between the two conductive columns, and the slots are arranged in parallel along the connecting line direction of the two conductive columns; the slot top is provided with the horizontal clamp plate of sliding from top to bottom, is connected with the cooling box through the rotating arm on the liquid reserve tank mouth edge of horizontal clamp plate both sides, and the cooling box has cooling position and application of sample position. The utility model has the advantages that the membrane transferring clamp loading of the PAGE gel and the PVDF membrane is easily realized in the electrophoresis liquid, the exposure of the PAGE gel and the PVDF membrane to the air is avoided, the entry of gas in the gap between the PAGE gel and the PVDF membrane is prevented, the damage of the electrophoresis membrane transferring process caused by the influence of the air is avoided, the membrane transferring effect is improved, and the accuracy of the experimental result is ensured.
Description
Technical Field
The utility model relates to the technical field of immunoblotting experiments, in particular to a protein electrophoresis gel membrane transferring device suitable for underwater operation.
Background
In performing western blot experiments (i.e., immunoblotting experiments), protein samples are added to a PAGE gel (i.e., polyacrylamide gel), and proteins of different molecular weights are then electrophoretically accumulated on different bands of the PAGE gel, for example: under the same voltage, proteins with small molecular weight have a high electrophoresis speed and accumulate in the lower layer of the PAGE gel, while proteins with large molecular weight have a low electrophoresis speed and accumulate in the upper layer of the PAGE gel.
After electrophoresis, proteins are transferred onto a PVDF film (namely a polyvinylidene fluoride film), then an antibody is coated, after antigen-antibody reaction, a developing solution is dripped on the PVDF film, and finally a film scanning instrument is used for scanning, so that the concentration of specific proteins in different samples is displayed, and further quantitative analysis is carried out on the proteins.
The conventional transfer operation at present is: first, a PAGE gel and PVDF membrane are placed between the two clamping plates of a membrane transfer clamp 100 (shown in FIG. 2) and the filter paper and the foam cushion are lined in front and back; then, clamping by using a film transfer clamp 100, and inserting the film transfer clamp 100 into a film transfer groove, wherein the front and back sides (the clamping plate on one side of the conventional film transfer clamp 100 on the market is black, the clamping plate on the other side of the conventional film transfer clamp is transparent, and the film transfer groove is black at one side, so that the film transfer clamp 100 and the film transfer groove are aligned), and one side of gel is required to correspond to the black clamping plate and the black side of the film transfer groove; then, applying constant current to carry out electrophoresis transfer film; during membrane transfer, current penetrates from the gel to the PVDF membrane, thereby transferring the proteins thereon to the PVDF membrane as well.
In the film transfer process, bubbles cannot be required to exist between the PAGE gel and the PVDF film, and if bubbles exist, the proteins cannot smoothly transfer to the PVDF film, so that experimental errors can be caused; in addition, the existing film is usually turned into a film by 300 mA for 1-2 hours, a large amount of heat is generated in the film transferring process, and the film transferring groove needs to be cooled.
In addition, when laying the PAGE gel and the PVDF film on the film transfer clip 100, the film transfer liquid is usually inverted into a tray, then a sponge pad and thick filter paper are laid on the film transfer clip 100 in sequence, then the PAGE gel is placed, and then the PVDF film is placed on the gel; the gel and the membrane are exposed to the air in the process, or only a small part of the gel and the membrane are placed in water, the membrane transferring clamp 100 is clamped after the membrane is laid, the membrane transferring clamp 100 is transferred from the membrane transferring liquid to be inserted into the membrane transferring groove, and the PAGE gel and the PVDF membrane are exposed to the air in the process, so that gas in a gap between the PAGE gel and the PVDF membrane is often caused to enter, the membrane transferring process is damaged, the membrane transferring effect is poor, and the experimental result is influenced.
Disclosure of Invention
The utility model aims to provide a protein electrophoresis gel transfer device suitable for underwater operation.
In order to achieve the above purpose, the present utility model may adopt the following technical scheme:
The utility model relates to a protein electrophoresis gel film transferring device suitable for underwater operation, which comprises a liquid storage tank with an upper opening structure and a tank cover which is adapted to be sealed and buckled on the liquid storage tank, wherein a pair of conductive columns are arranged in the liquid storage tank at intervals, the upper ends of the conductive columns vertically extend upwards to penetrate out of the tank cover, a plurality of slots for inserting a film transferring clamp are arranged on the bottom surface of the liquid storage tank between the two conductive columns, and the slots are arranged in parallel along the connecting line direction of the two conductive columns; the cooling box is characterized in that a horizontal pressing plate which slides up and down along the wall of the liquid storage box is arranged above the slot, a cooling box for containing cooling liquid is connected to the opening edge of the liquid storage box at two sides of the horizontal pressing plate through a rotating arm, and the cooling box is driven by the rotating arm to have a cooling position for rotating into the liquid storage box and a sample feeding position for rotating out of the liquid storage box.
The utility model has the advantages that the membrane transferring clamp loading of the PAGE gel and the PVDF membrane is easily realized in the electrophoresis liquid by the slot which is arranged in the liquid storage tank and is used for vertically inserting the membrane transferring clamp and matching with the horizontal pressing plate above the slot; meanwhile, the cooling box which can be transferred into the liquid storage box is matched to cool the electrophoresis liquid, so that the box cover can be directly buckled for electrophoresis; the whole process can ensure that the PAGE gel, the PVDF film and the film transferring clamp are always operated in the electrophoresis liquid, avoid exposing the PAGE gel and the PVDF film in the air, prevent the air from entering the gap between the PAGE gel and the PVDF film, avoid being influenced by the air to cause the damage of the electrophoresis film transferring process, improve the film transferring effect and ensure the accuracy of experimental results.
Specifically, set up vertical spout on the inside wall of liquid reserve tank, slide in the vertical spout be provided with the slider that horizontal clamp plate links firmly mutually, easily realize horizontal clamp plate along the upper and lower translation slip of the wall of liquid reserve tank through vertical spout and the slider of mutually supporting.
Further, for stability when the horizontal pressing plate moves horizontally up and down, the vertical sliding grooves are symmetrically formed in two groups on the same side wall of the liquid storage tank, and each vertical sliding groove is internally provided with one sliding block in a sliding mode.
Furthermore, water permeable strip holes are uniformly distributed on the horizontal pressing plate, so that the upper and lower translation of the horizontal pressing plate can not disturb the electrophoresis liquid in the liquid storage tank.
Furthermore, the cooling box is provided with a liquid injection port, and the liquid injection port is provided with a sealing cover in an adaptive sealing manner, so that cooling liquid can be conveniently filled into the cooling box or replaced.
Further, the bottom of the liquid storage tank is provided with a cooling seat, a power connection wire is arranged on the cooling seat, and the continuous low temperature of the electrophoresis liquid in the liquid storage tank can be easily ensured through the cooling seat.
Drawings
Fig. 1 is a schematic structural view of the present utility model.
Fig. 2 is a schematic structural view of a conventional film transfer clip.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
It should be noted that all directional indicators (such as up, down, left, right, front, and rear … …) in the embodiments of the present utility model are merely used to explain the relative positional relationship, movement, etc. between the components in a particular posture (as shown in the drawings), and if the particular posture is changed, the directional indicator is changed accordingly.
In addition, in the present utility model, unless explicitly stated and limited otherwise, the terms "connected," "secured," and the like are to be construed broadly, and for example, "secured" may be a fixed connection, a removable connection, or an integral body; can be mechanically or electrically connected; either directly or indirectly, through intermediaries, or both, may be in communication with each other or in interaction with each other, unless expressly defined otherwise. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.
As shown in fig. 1, the protein electrophoresis gel transfer device suitable for underwater operation comprises a liquid storage tank 1 with an upper opening structure and a tank cover 2 which is adapted to be sealed and buckled on the liquid storage tank 1; preferably, the liquid storage tank 1 has a rectangular structure, and at this time, the chamber inside the liquid storage tank 1 is also a rectangular chamber with an upper opening, and the tank cover 2 matched with the liquid storage tank 1 is also a rectangular cover body.
A pair of conductive columns 3 are arranged at intervals in the middle of the rectangular cavity of the liquid storage tank 1, and the two conductive columns 3 are arranged at intervals along the width direction of the liquid storage tank 1 and are respectively stuck to the inner sides of the side walls of the two long sides of the liquid storage tank 1; the upper end of the conductive post 3 extends vertically upwards out of the case cover 2, namely: the case cover 2 is provided with a pair of perforations 4 which are in one-to-one correspondence with the two conductive columns 3, so that when the case cover 2 is buckled on the liquid storage case 1, the upper ends of the conductive columns 3 just can penetrate out of the perforations 4, and then the case cover 2 is conveniently buckled, and then an electrode plug is directly connected to the conductive columns 3 on the outer side of the case cover 2 for electrophoresis operation.
A plurality of slots 5 for inserting a film transferring clip 100 (shown in fig. 2) are arranged on the bottom surface of the liquid storage tank 1 between the two conductive columns 3, and the slots 5 are arranged in parallel along the connecting line direction of the two conductive columns 3, namely: the length direction of each slot 5 is consistent with the length direction of the liquid storage tank 1.
A horizontal pressing plate 6 which slides up and down along the wall of the liquid storage tank 1 is arranged above the slot 5, specifically, a vertical sliding groove 7 can be formed on the inner side wall of the liquid storage tank 1, and a sliding block 8 fixedly connected with the horizontal pressing plate 6 is arranged in the vertical sliding groove 7 in a sliding manner, so that the horizontal pressing plate 6 can easily slide up and down along the wall of the liquid storage tank 1 in a sliding manner through the mutual matching of the vertical sliding groove 7 and the sliding block 8; of course, in order to enhance the stability of the horizontal pressing plate 6 during the vertical sliding, the vertical sliding grooves 7 should be two groups symmetrically arranged on the same side wall of the liquid storage tank 1, and a sliding block 8 is slidably arranged in each vertical sliding groove 7. In addition, in order to ensure that the up-and-down translation of the horizontal pressing plate 6 does not disturb the electrophoretic fluid in the liquid storage tank 1, a plurality of water permeable strip holes 9 are uniformly distributed on the horizontal pressing plate 6; of course, since the thickness of the PAGE gel and PVDF film is generally not more than 1.5mm, the width of the water permeable strip hole 9 should be greater than 1.5mm, and the length of the water permeable strip hole 9 should be slightly greater than the length of the PAGE gel and PVDF film so as to be able to put the PAGE gel and PVDF film in the water permeable strip hole 9.
In addition, the opening edge of the liquid storage tank 1 positioned at two sides of the horizontal pressing plate 6 is also connected with a cooling box 11 for containing cooling liquid through a rotating arm 10, the cooling box 11 is driven by the rotating arm 10 to have a cooling position which is shifted into the liquid storage tank 1 and a sample adding position which is shifted out of the liquid storage tank 1, and when the cooling box 11 is positioned at the cooling position, the rotating arm 10 and the cooling box 11 are positioned in the liquid storage tank 1, and meanwhile, the rotating arm 10 and the cooling box 11 are positioned below the upper opening edge of the liquid storage tank 1 so as to ensure that the locking of the box cover 2 is not influenced. Of course, in order to facilitate filling or replacing cooling liquid into the cooling box 11 easily, the cooling box 11 should also be provided with a liquid filling port 12, and the liquid filling port 12 is adapted with a sealing cover 13.
Further, in order to easily keep the continuous low temperature of the electrophoresis liquid in the liquid storage tank 1, a cooling seat 14 can be further arranged at the bottom of the liquid storage tank 1, and a power connection wire 15 which can be connected with an external power supply is arranged on the cooling seat 14, so that the cooling seat 14 is connected with the power supply through the power connection wire 15, and then the cooling seat 14 is opened and adjusted to a proper temperature, so that the electrophoresis liquid in the liquid storage tank 1 is continuously cooled, and the cooling of the PAGE gel and the PVDF film in the film transfer clamp 100 is continuously ensured in the electrophoresis film transfer process.
When in use, the case cover 2 is opened, the cooling box 11 is rotated to the sample adding position, and the electrophoresis liquid is poured into the liquid storage case 1; then the horizontal pressing plate 6 can be translated upwards to leave enough space for the lower part, and the PAGE gel, the PVDF film and the film transferring clamp 100 are put into the liquid storage tank 1 below the horizontal pressing plate 6, so that the clamping process of the PAGE gel and the PVDF film is completed in the electrophoresis liquid; after the clamping is completed, the film transfer clamp 100 can be vertically inserted into the slot 5 (taking care of ensuring that the front and back sides of the film transfer clamp 100 meet the requirements), and the horizontal pressing plate 6 is translated downwards to fix the film transfer clamp 100; then filling the cooling box 11 with the refrigerating fluid, and buckling the sealing cover 13 to rotate the cooling box 11 to a cooling position; then the case cover 2 can be buckled, and electrode plugs are connected to the two conductive posts 3 extending out of the case cover 2 to carry out electrophoresis film transferring operation, and the temperature reduction and 14 can be continuously opened in the electrophoresis process so as to keep the continuous low temperature of the electrophoresis liquid in the liquid storage case 1.
The whole protein electrophoresis gel film transferring device can easily realize the loading of the film transferring clamp 100 for finishing the PAGE gel and the PVDF film in the electrophoresis liquid, ensure that the PAGE gel, the PVDF film and the film transferring clamp 100 are always operated in the electrophoresis liquid, avoid exposing the PAGE gel and the PVDF film in the air, prevent the air from entering the gap between the PAGE gel and the PVDF film, avoid being influenced by the air to cause the damage of the electrophoresis film transferring process, improve the film transferring effect and ensure the accuracy of experimental results.
Claims (6)
1. A protein electrophoresis gel transfer device suitable for underwater operation, which is characterized in that: the liquid storage box comprises a liquid storage box with an upper opening structure and a box cover which is adapted to be sealed and buckled on the liquid storage box, wherein a pair of conductive columns are arranged in the liquid storage box at intervals, the upper ends of the conductive columns vertically extend upwards to penetrate out of the box cover, a plurality of slots for inserting a rotating film clamp are formed in the bottom surface of the liquid storage box between the two conductive columns, and the slots are arranged in parallel along the connecting line direction of the two conductive columns; the cooling box is characterized in that a horizontal pressing plate which slides up and down along the wall of the liquid storage box is arranged above the slot, a cooling box for containing cooling liquid is connected to the opening edge of the liquid storage box at two sides of the horizontal pressing plate through a rotating arm, and the cooling box is driven by the rotating arm to have a cooling position for rotating into the liquid storage box and a sample feeding position for rotating out of the liquid storage box.
2. The protein electrophoresis gel transfer device adapted for underwater operation according to claim 1, wherein: a vertical sliding groove is formed in the inner side wall of the liquid storage tank, and a sliding block fixedly connected with the horizontal pressing plate is arranged in the vertical sliding groove in a sliding mode.
3. The protein electrophoresis gel transfer device adapted for underwater operation according to claim 2, wherein: the vertical sliding grooves are symmetrically formed in two groups on the same side wall of the liquid storage tank, and each vertical sliding groove is internally provided with one sliding block in a sliding manner.
4. The protein electrophoresis gel transfer device adapted for underwater operation according to claim 1, wherein: and water permeable strip holes are uniformly distributed on the horizontal pressing plate.
5. The protein electrophoresis gel transfer device adapted for underwater operation according to claim 1, wherein: the cooling box is provided with a liquid injection port, and a sealing cover is matched and buckled on the liquid injection port.
6. The protein electrophoresis gel transfer device adapted for underwater operation according to claim 1, wherein: the liquid storage tank bottom is provided with the cooling seat, be provided with the power connecting wire on the cooling seat.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322915046.3U CN221572418U (en) | 2023-10-30 | 2023-10-30 | Protein electrophoresis gel film transferring device suitable for underwater operation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322915046.3U CN221572418U (en) | 2023-10-30 | 2023-10-30 | Protein electrophoresis gel film transferring device suitable for underwater operation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN221572418U true CN221572418U (en) | 2024-08-20 |
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ID=92272103
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322915046.3U Active CN221572418U (en) | 2023-10-30 | 2023-10-30 | Protein electrophoresis gel film transferring device suitable for underwater operation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN221572418U (en) |
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2023
- 2023-10-30 CN CN202322915046.3U patent/CN221572418U/en active Active
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