CN212503919U - Stem cell culture medium partial shipment device - Google Patents
Stem cell culture medium partial shipment device Download PDFInfo
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- CN212503919U CN212503919U CN202021138385.5U CN202021138385U CN212503919U CN 212503919 U CN212503919 U CN 212503919U CN 202021138385 U CN202021138385 U CN 202021138385U CN 212503919 U CN212503919 U CN 212503919U
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- culture medium
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- medium storage
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Abstract
The utility model provides a stem cell culture medium partial shipment device, concretely relates to biological medium technical field. The utility model comprises a culture medium storage bottle, wherein a bottle cap is arranged at the bottle mouth of the culture medium storage bottle, and a vent pipe is arranged on the bottle cap; a piston is arranged in the culture medium storage bottle, and the edge of the piston is in sliding connection with the inner side wall of the culture medium storage bottle; the piston is provided with a pull rod, the pull rod is provided with volume scale marks and penetrates through the bottle cap, and the pull rod is connected with the bottle cap and the piston in a sliding manner; be equipped with the transfer line in the culture medium apotheca, the transfer line runs through piston and bottle lid, transfer line and piston sliding connection, and the bottle bottom of culture medium apotheca is located to the one end of transfer line, and the outside of culture medium apotheca is located to the other end of transfer line, is equipped with the valve on the transfer line. The utility model provides a stem cell culture medium partial shipment device can prevent that the liquid in the culture medium storage bottle from receiving the pollution at the in-process of culture medium partial shipment, guarantees the accuracy of experimental result.
Description
Technical Field
The utility model belongs to the technical field of biological medium, concretely relates to stem cell culture medium partial shipment device.
Background
In the process of biological subculture, the culture medium needs to be subpackaged and replaced for many times. At present, the experimental operation of the split charging of the culture medium is divided into two types, one type is to open the bottle cap of a culture medium storage bottle, a liquid transfer device extends into the bottle to take liquid, and the other type is to directly pour the liquid in the bottle. However, these two operations easily cause the culture medium in the bottle to be polluted, which affects the experimental results. Therefore, there is a need for a stem cell culture medium dispensing device that can solve the above problems.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a stem cell culture medium partial shipment device can prevent that the liquid in the culture medium storage bottle from receiving the pollution at the in-process of culture medium partial shipment, guarantees the accuracy of experimental result.
The utility model provides a following technical scheme:
a stem cell culture medium subpackaging device comprises a culture medium storage bottle, wherein a bottle cap is arranged at the bottle mouth of the culture medium storage bottle, and a vent pipe is arranged on the bottle cap; a piston is arranged in the culture medium storage bottle, and the edge of the piston is in sliding connection with the inner side wall of the culture medium storage bottle; a pull rod is arranged on the piston, volume scale marks are arranged on the pull rod, the pull rod penetrates through the bottle cap, and the pull rod is connected with the bottle cap and the piston in a sliding mode; the culture medium storage bottle is characterized in that a liquid conveying pipe is arranged in the culture medium storage bottle and penetrates through the piston and the bottle cap, the liquid conveying pipe is in sliding connection with the piston, one end of the liquid conveying pipe is arranged at the bottom of the culture medium storage bottle, the other end of the liquid conveying pipe is arranged on the outer side of the culture medium storage bottle, and a valve is arranged on the liquid conveying pipe.
Preferably, one end of the vent pipe is communicated with the bottle cap, one end of the vent pipe is provided with a microbial filter, and the other end of the vent pipe is provided with the valve.
Preferably, the lower end of the pull rod is provided with a clamping plate, and the end face of the clamping plate is connected with the pull rod.
Preferably, the upper end of the piston is provided with a cavity matched with the clamping plate, and the cavity wall of the cavity is rotatably connected with the clamping plate.
Preferably, the edge of the piston is provided with a groove.
Preferably, the outer side wall of the pull rod is provided with threads, and the pull rod is in threaded connection with the bottle cap.
Preferably, the outer side wall of the infusion tube is provided with a fixing ring, and the inner side wall of the fixing ring is connected with the infusion tube.
Preferably, a clamping groove matched with the fixing ring is arranged in the bottle cap, and the groove wall of the clamping groove is connected with the fixing ring.
The utility model discloses a profitable effect:
the bottle mouth of the culture medium storage bottle is provided with the bottle cap, the bottle cap is provided with the vent pipe, the piston is arranged in the culture medium storage bottle, the edge of the piston is in sliding connection with the inner side wall of the culture medium storage bottle, the vent pipe can keep the air pressure inside and outside the culture medium storage bottle the same, the piston can move conveniently, and the piston can extrude the liquid in the culture medium storage bottle;
the piston is provided with a pull rod, the pull rod is provided with volume scale marks and penetrates through the bottle cap, the pull rod is connected with the bottle cap and the piston in a sliding mode, the pull rod is convenient for the piston to move, and the volume scale marks can read the volume of liquid in the culture medium storage bottle;
be equipped with the transfer line in the culture medium storage bottle, the transfer line runs through piston and bottle lid, the bottle end of culture medium storage bottle is located to the one end of transfer line, the outside of culture medium storage bottle is located to the other end of transfer line, be equipped with the valve on the transfer line, pass through the transfer line outflow by extruded liquid, the switch of the steerable transfer line of valve, liquid in the whole partial shipment process culture medium storage bottle does not have with external gas contact, liquid in the culture medium storage bottle has been prevented from receiving the pollution, guarantee the accuracy of experimental result.
Drawings
The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description serve to explain the invention and not to limit the invention.
In the drawings:
fig. 1 is a schematic structural diagram of the present invention;
description of reference numerals:
1. a culture medium storage bottle; 2. a bottle cap; 3. a breather pipe; 4. a piston; 5. a pull rod; 6. volume scale lines; 7. a transfusion tube; 8. a valve; 9. a microbial filter; 10. clamping a plate; 11. a concave cavity; 12. a fixing ring; 13. a clamping groove.
Detailed Description
As shown in figure 1, a stem cell culture medium subpackaging device comprises a culture medium storage bottle 1, a bottle cap 2 is arranged at the bottle mouth of the culture medium storage bottle 1, a through vent pipe 3 is arranged on the bottle cap 2, one end of the vent pipe 3 is connected with the bottle cap 1, a microbial filter 9 is arranged at one end of the vent pipe 3, a valve 8 is arranged at the other end of the vent pipe 3, a piston 4 is arranged in the culture medium storage bottle 1, the edge of the piston 4 is in sliding connection with the inner side wall of the culture medium storage bottle 1, a groove 11 is arranged at the edge of the piston 4, the groove 11 can reduce the friction force between the piston 4 and the culture medium storage bottle 1, the piston 4 can be conveniently moved, the vent pipe 3 can keep the same air pressure inside and outside the culture medium storage bottle 1, the piston 4 can be conveniently moved, the valve 8 can control the opening and closing of the vent pipe 3, the microbial filter 9, the piston 4 can press the liquid in the medium storage bottle 1.
The upper end of the piston 4 is provided with a pull rod 5, the lower end of the pull rod 5 is provided with a clamping plate 10, the upper end face of the clamping plate 10 is connected with the pull rod 5, the upper end of the piston 4 is provided with a cavity 11 matched with the clamping plate 10, the cavity wall of the cavity 11 is rotatably connected with the clamping plate 10, the pull rod 5 is provided with volume scale marks 6, the pull rod 5 penetrates through the bottle cap 2, the outer side wall of the pull rod 5 is provided with threads 12, the pull rod 5 is in threaded connection with the bottle cap 2, the threads 12 are convenient for the pull rod 5 to slowly push the piston 4 to move, the volume of liquid in the culture medium storage bottle 1 can be read by the volume scale marks 6, and the; a liquid conveying pipe 7 is arranged in the culture medium storage bottle 1, the liquid conveying pipe 7 penetrates through the piston 4 and the bottle cap 2, the liquid conveying pipe 7 is connected with the piston 4 in a sliding mode, one end of the liquid conveying pipe 7 is arranged at the bottom of the culture medium storage bottle 1, the other end of the liquid conveying pipe 7 is arranged on the outer side of the culture medium storage bottle 1, a valve 8 is arranged on the liquid conveying pipe 7, extruded liquid flows out through the liquid conveying pipe 7, and the valve 8 can control the liquid conveying pipe 7 to be; the outer side wall of the infusion tube 7 is provided with a fixing ring 12, the inner side wall of the fixing ring 12 is connected with the infusion tube 7, a clamping groove 13 matched with the fixing ring is arranged in the bottle cap 2, the groove wall of the clamping groove 13 is connected with the fixing ring 12, and the fixing ring 12 and the clamping groove 13 enable the infusion tube 7 to be fixed more firmly.
The utility model discloses a working method: open valve 8 on breather pipe 3 and the transfer line 7, rotate pull rod 5, piston 4 slowly moves down, and the liquid in the culture medium storage bottle 1 passes through transfer line 7 and flows out, through the change of observing the volume scale mark around the partial shipment, calculates the liquid volume that flows out, and the liquid in the whole partial shipment process culture medium storage bottle 1 does not contact with external gas, has prevented that the liquid in the culture medium storage bottle 1 from receiving the pollution, guarantees the accuracy of experimental result.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described in the foregoing embodiments, or equivalents may be substituted for elements thereof. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (8)
1. The utility model provides a stem cell culture medium partial shipment device, includes the culture medium storage bottle, its characterized in that: a bottle cap is arranged at the bottle mouth of the culture medium storage bottle, and a vent pipe is arranged on the bottle cap; a piston is arranged in the culture medium storage bottle, and the edge of the piston is in sliding connection with the inner side wall of the culture medium storage bottle; a pull rod is arranged on the piston, volume scale marks are arranged on the pull rod, the pull rod penetrates through the bottle cap, and the pull rod is connected with the bottle cap and the piston in a sliding mode; the culture medium storage bottle is characterized in that a liquid conveying pipe is arranged in the culture medium storage bottle and penetrates through the piston and the bottle cap, the liquid conveying pipe is in sliding connection with the piston, one end of the liquid conveying pipe is arranged at the bottom of the culture medium storage bottle, the other end of the liquid conveying pipe is arranged on the outer side of the culture medium storage bottle, and a valve is arranged on the liquid conveying pipe.
2. The stem cell culture medium dispensing device of claim 1, wherein: one end of the vent pipe is communicated with the bottle cap, one end of the vent pipe is provided with a microbial filter, and the other end of the vent pipe is provided with the valve.
3. The stem cell culture medium dispensing device of claim 1, wherein: the lower end of the pull rod is provided with a clamping plate, and the end face of the clamping plate is connected with the pull rod.
4. The stem cell culture medium dispensing device of claim 3, wherein: the upper end of the piston is provided with a cavity matched with the clamping plate, and the cavity wall of the cavity is rotatably connected with the clamping plate.
5. The stem cell culture medium dispensing device of claim 1, wherein: the edge of the piston is provided with a groove.
6. The stem cell culture medium dispensing device of claim 1, wherein: the outer side wall of the pull rod is provided with threads, and the pull rod is in threaded connection with the bottle cap.
7. The stem cell culture medium dispensing device of claim 1, wherein: the outer side wall of the infusion tube is provided with a fixing ring, and the inner side wall of the fixing ring is connected with the infusion tube.
8. The stem cell culture medium dispensing device of claim 7, wherein: the bottle cap is internally provided with a clamping groove matched with the fixing ring, and the groove wall of the clamping groove is connected with the fixing ring.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021138385.5U CN212503919U (en) | 2020-06-18 | 2020-06-18 | Stem cell culture medium partial shipment device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021138385.5U CN212503919U (en) | 2020-06-18 | 2020-06-18 | Stem cell culture medium partial shipment device |
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| Publication Number | Publication Date |
|---|---|
| CN212503919U true CN212503919U (en) | 2021-02-09 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021138385.5U Active CN212503919U (en) | 2020-06-18 | 2020-06-18 | Stem cell culture medium partial shipment device |
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| Country | Link |
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| CN (1) | CN212503919U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115232713A (en) * | 2022-09-02 | 2022-10-25 | 安徽大学 | Portable laboratory simple and easy device of oxygen in getting rid of culture medium |
-
2020
- 2020-06-18 CN CN202021138385.5U patent/CN212503919U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115232713A (en) * | 2022-09-02 | 2022-10-25 | 安徽大学 | Portable laboratory simple and easy device of oxygen in getting rid of culture medium |
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