CN212270121U

CN212270121U - Portable frozen cell recovery box

Info

Publication number: CN212270121U
Application number: CN202020553767.8U
Authority: CN
Inventors: 郑晓佳
Original assignee: Tianjin Kangting Biological Engineering Group Co Ltd
Current assignee: Tianjin Kangting Biological Engineering Group Co Ltd
Priority date: 2020-04-15
Filing date: 2020-04-15
Publication date: 2021-01-01
Anticipated expiration: 2030-04-15

Abstract

The utility model relates to a portable cryopreservation cell resuscitation box, which comprises a base, a pot body frame arranged on the base, a lead screw frame arranged on the base, a water bath pot arranged on the pot body frame and a circulating water mechanism arranged on the left side of the water bath pot; be provided with the lead screw on the upper portion crossbeam of lead screw frame, the servo motor is equipped with admittedly on the lead screw, servo motor lower extreme coaxial arrangement pivot, the pivot extends to insert in the water bath, and the pivot tip is equipped with one admittedly and freezes a tub recovery basket, freezes to deposit tub recovery basket lead screw and drives servo motor and reciprocate, and servo motor drive freezes to deposit tub recovery basket internal rotation in the water bath. Combine together lead screw and servo motor and circulating water mechanism, utilize control panel can realize the recovery operation, the work load that significantly reduces effectively ensures that the cell strain is whole to be melted in one minute, improves work efficiency, and the cell strain in each cryopreserved pipe melts the rate unanimous basically, guarantees the survival rate of cell.

Description

Portable frozen cell recovery box

Technical Field

The utility model belongs to the technical field of the experiment auxiliary assembly, a cryopreserved cell resuscitation technique is related to, especially a portable cryopreserved cell resuscitation box.

Background

Cells are indispensable research objects in the field of modern life science research, have irreplaceable effects on medical and pharmacological research, and the medical clinic gradually uses stem cells for health care of human bodies and immune cells for treatment of diseases. From in vitro cell experiments to in vivo cell experiments, all links have little participation of cells, the survival rate of the cells in the experimental processes has vital influence on the success or failure of the whole experiment and treatment, different methods of cell preservation and resuscitation can seriously influence the survival rate of the cells, and further the vitality of the cells has great influence on the research of life science.

At present, the cell recovery is realized by regulating the water temperature of a water bath kettle to 38-41 ℃, cells are thawed by using a buoy or a laboratory worker to hold a cell freezing tube in the water bath kettle to shake, the water bath kettle is heavy and cannot move at any time, the temperature can not be accurately controlled, and the laboratory worker is very easy to immerse the tube opening of the freezing tube into the water of the water bath kettle when holding the freezing tube in the hand, so that the possibility of cell pollution is increased; when the buoy is used, on one hand, the loading and unloading of the buoy are time-consuming and trouble-consuming, on the other hand, the position of the fixed freezing storage pipe of the buoy is too tight and cannot be in direct contact with water, so that cells at the position are not easy to freeze, the recovery time is too long, and the cell activity is reduced.

The following patent documents relevant to the present application are found by search, and specific disclosures are as follows:

1. portable cryopreserved cell recovery box (CN209652321U), comprises a box bod, the box inner chamber is equipped with the fixed disk, and evenly is equipped with vertical through-hole in the fixed disk to alternate in the vertical through-hole and have cryopreserved pipe, the fixed disk outside is equipped with the step through-hole, and the step through-hole communicates with each other with vertical through-hole to alternate in the step through-hole has the connecting rod, the connecting rod sets up to "T" shape structure, and connecting rod outer wall cover has the spring to the spring external diameter is greater than fixed disk step through-hole one side internal diameter, connecting rod one side is equipped with the mount that is connected, and the mount sets up to "V" type structure, the fixed disk lower extreme is.

2. The utility model provides a laboratory is with portable freezing cell recovery box (CN205473725U), includes the box body, installs the support in the box body of taking the hole, installs at least one anchor clamps that can fixed cell freezing tube on the support, is located to be equipped with temperature control system on the box body inner wall of support below, still is equipped with the heater in the box body, and temperature control system links to each other with the heater for give the water heating in the box body.

3. Novel cell recovery device (CN210134124U), including jar body, cover and cryopreserving a tub recovery basket, jar side wall middle part is filled the heat preservation, jar body side-mounting drain pipe, jar body upper portion is sealed to be provided with cover, its characterized in that: the middle part of the tank cover is coaxially and penetratingly hinged with a rotating shaft through a shaft sleeve, the upper end of the rotating shaft is fixedly provided with a handle, the lower end of the rotating shaft is coaxially hinged with a cryopreservation tube recovery basket, a spring barrel is arranged on the rotating shaft between the handle and the cryopreservation tube recovery basket, the spring barrel is coaxially arranged on the upper surface of the tank cover, a spring is arranged in the spring barrel, the outer end of the spring is fixedly arranged on the fixed end of the inner side wall of the spring barrel, the inner end of the spring is fixedly arranged on the rotating shaft, the stress of the spring drives the cryopreservation tube recovery basket to rotate in the tank body, and cells in the cryopreservation tube are heated uniformly and are molten; the utility model discloses a recovery basket, including resuscitation basket body and fixed picture peg, the freezing pipe resuscitation basket of depositing includes resuscitation basket body and fixed picture peg, this internal lower part of resuscitation basket is provided with fixed picture peg, resuscitation basket body upper surface equipartition interval system has a plurality of circular freezing pipe recess of depositing and deposits the hole, freezing pipe recess of depositing downthehole inlay the dress and freeze and deposit the pipe, it still makes to freeze and deposits a tub draw-in groove to correspond to freeze on depositing the fixed picture peg that the pipe recess was deposited the hole position, the side from last equipartition interval system of resuscitation basket body has a plurality of inlet openings.

Through the contrast of technical characteristics, above-mentioned patent literature of disclosing with the utility model discloses a technical structure is inequality, can not influence the creativity and the novelty of the utility model application.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to overcome prior art's weak point, provide a simple structure, keep warm effectual, constancy of temperature, melt efficiency fast and labour saving and time saving's portable cryopreserved cell recovery box.

The utility model provides a its technical problem take following technical scheme to realize:

a portable cryopreservation cell recovery box comprises a base, a pot body frame arranged on the base, a screw rod frame arranged on the base, a water bath pot arranged on the pot body frame, and a circulating water mechanism arranged on the left side of the water bath pot; the water bath includes the pot body, sets up at the insulation cover of the external side of pot, sets up in the constant temperature heating ring and the pot lid of coaxial arrangement in pot body upper end of bottom in the pot body, be provided with the lead screw on the upper portion crossbeam of lead screw frame, be equipped with servo motor on the lead screw admittedly, servo motor lower extreme coaxial arrangement pivot, in the pivot extension inserts the water bath, the pivot tip is equipped with one and freezes a tub recovery basket admittedly, it reciprocates to freeze tub recovery basket lead screw drive servo motor, and the servo motor drive freezes deposits tub recovery basket at the water bath internal rotation.

And, circulating water mechanism include with the drain pipe of pot body bottom intercommunication with the inlet tube of pot body upper portion intercommunication and connection the water pump of drain pipe and inlet tube, the water inlet of drain pipe is located the below position of constant temperature heating ring.

And, circulating water mechanism still include with the moisturizing pipe of drain pipe intercommunication, all installed the electromagnetism on moisturizing pipe, drain pipe and the inlet tube.

And, it manages recovery basket including recovery basket body and fixed picture peg to freeze to deposit, the side from last equipartition interval system of recovery basket body has a plurality of inlet openings, this internal lower part of recovery basket is provided with fixed picture peg, recovery basket body upper surface equipartition interval system has a plurality of circular freezing to deposit tub recess and deposits the hole, it deposits downthehole the inlaying of dress and freezes to deposit the pipe to freeze to deposit tub recess, and it deposits the fixed picture peg of hole position and still to make on the fixed picture peg of hole position to correspond to freeze to deposit tub recess and be used for the fixed freezing of depositing tub bottom portion and deposit tub draw-in groove.

And a detachable thermometer and a temperature sensor are arranged in the water bath kettle.

And, still be provided with control panel on the lead screw frame, the last display screen that installs of control panel, control panel respectively with servo motor, water pump, constant temperature heating ring and temperature sensor link to each other.

And the lower end surface of the outer edge of the pot cover is provided with an elastic rubber ring.

The utility model has the advantages that:

1. the utility model discloses simple structure, design benefit combines together lead screw and servo motor and circulating water mechanism, utilizes control panel to realize the operation of coming back to life or consciousness promptly, and the work load that significantly reduces effectively ensures that the cell trunk is whole to be melted in one minute, improves work efficiency, and each freezes the intraductal cell trunk of depositing and melts rate unanimous basically, guarantees the survival rate of cell.

2. The utility model discloses a cell cryopreserving pipe can be rotatory in 37 ℃ water bath, and the cell thawing speed is fast, efficient, and through cryopreserving a tub draw-in groove of cryopreserving tub recovery basket with cryopreserve a tub recess and deposit the fixed pipe of cryopreserving of hole cooperation, stability is strong.

3. The utility model discloses a combination of insulation cover, circulating water mechanism and constant temperature heating ring has realized the waters temperature control when the cell is come back to life or consciousness, for the cell is come back to life or consciousness provides required constant temperature waters, utilizes the utility model discloses when carrying out the cell and come back to life or consciousness, can improve the security in the operation process, ensure cell and come back to life or consciousness efficiency and cell stability, utilize the utility model discloses a many batches of cells of coming back to life or consciousness simultaneously temporary storage device can come back to life or consciousness, provides necessary experimental apparatus for experiment and scientific research cell come back to life or consciousness.

Drawings

Fig. 1 is a schematic structural diagram of the present invention.

Detailed Description

The present invention will be described in further detail with reference to the following embodiments, which are illustrative, not restrictive, and the scope of the invention should not be limited thereto.

A portable cryopreservation cell recovery box comprises a base 18, a pot body frame 13 arranged on the base, a lead screw frame 5 arranged on the base, a water bath pot arranged on the pot body frame and a circulating water mechanism arranged on the left side of the water bath pot; the water bath kettle comprises a kettle body 8, a heat insulation sleeve 12 arranged on the outer side of the kettle body, a constant temperature heating ring arranged at the bottom in the kettle body and a kettle cover 6 coaxially arranged at the upper end of the kettle body, wherein a screw rod frame is correspondingly arranged at the upper part of the water bath kettle, a screw rod is arranged on a cross beam at the upper part of the screw rod frame, a servo motor 1 is fixedly arranged on the screw rod, a rotating shaft 2 is coaxially arranged at the lower end of the servo motor, the rotating shaft is extended and inserted into the water bath kettle, a freezing tube recovery basket 9 is fixedly arranged at the end part of the rotating shaft, the freezing tube recovery basket is coaxially arranged in the water bath kettle and is not contacted with the bottom of the kettle body, the screw rod of the freezing tube recovery basket drives;

the circulating water mechanism comprises a drain pipe 16 communicated with the bottom of the pot body, a water inlet pipe 20 communicated with the upper part of the pot body and a water pump 19 connected with the drain pipe and the water inlet pipe, wherein a water inlet of the drain pipe is positioned below the heating ring, so that water heated by the constant-temperature heating ring at the bottom of the pot body is conveyed to the upper part of the pot body through the drain pipe and the water inlet pipe in sequence by the water pump;

in this embodiment, the circulating water mechanism further comprises a water replenishing pipe 14 communicated with the water discharging pipe, and

electromagnetic valves

17 and 15 are arranged on the water replenishing pipe, the water discharging pipe and the water inlet pipe. The water in the pot body is kept to flow circularly through the circulating water mechanism, and the circulating water is kept to be at a constant temperature through the constant-temperature heating ring, so that the operation is simple, and the use is safe and reliable;

in the specific implementation of the utility model, the cryopreservation tube resuscitation basket comprises a resuscitation basket body and a fixed inserting plate 10, the resuscitation basket body is cylindrical, a plurality of water inlet holes are uniformly distributed on the side surface of the resuscitation basket body from top to bottom at intervals, so that the resuscitation basket body is soaked with water, the fixed inserting plate is arranged on the lower part in the resuscitation basket body, a plurality of circular cryopreservation tube groove storage holes are uniformly distributed on the upper surface of the resuscitation basket body at intervals, the cryopreservation tubes 11 are embedded in the cryopreservation tube groove storage holes, and cryopreservation tube clamping grooves are also formed on the fixed inserting plate corresponding to the positions of the cryopreservation tube groove storage holes and can fix the bottoms of the cryopreservation tubes, so that the cryopreservation tubes are prevented from rotating in the cryopreservation tube groove storage holes and are prevented from being separated from the resuscitation basket body by buoyancy of the cryopreservation tube water;

the detachable thermometer 7 and the temperature sensor are arranged in the water bath kettle of the utility model, which is convenient for measuring the water temperature;

the utility model discloses in the implementation, still be provided with control panel 4 on the lead screw frame, control panel is last to install display screen 3, control panel respectively with motor, water pump, constant temperature heating ring and temperature sensor link to each other, have realized through control panel that the temperature is visual, and the heating is even, and the work load that significantly reduces effectively ensures that the cell strain is whole to melt in a minute, improves work efficiency, and each freezes the intraductal cell strain of depositing and melts rate unanimous basically, guarantees the survival rate of cell.

In this embodiment, the lower terminal surface of pot cover outward flange is provided with the elastic rubber circle, and during the use, the pot cover passes through the elastic rubber circle to be connected with pot body top, has avoided servo motor vibrations to the influence of the pot body in operation process.

The utility model discloses when doing cell resuscitation and melting, cover the pot cover on the pot body, and the experimenter can keep the certain distance with the water-bath, effectively reduces the probability that the frozen stock explodes the pipe injury experimenter, ensures experimenter safety.

The utility model discloses a cell cryopreserving pipe can be rotatory in 37 ℃ water bath, and the cell thawing speed is fast, efficient, and through cryopreserving a tub draw-in groove of cryopreserving tub recovery basket with cryopreserve a tub recess and deposit the fixed pipe of cryopreserving of hole cooperation, stability is strong.

The utility model discloses a combination of insulation cover, circulating water mechanism and constant temperature heating ring has realized the waters temperature control when the cell is come back to life or consciousness, for the cell is come back to life or consciousness provides required constant temperature waters, utilizes the utility model discloses when carrying out the cell and come back to life or consciousness, can improve the security in the operation process, ensure cell and come back to life or consciousness efficiency and cell stability, utilize the utility model discloses a many batches of cells of coming back to life or consciousness simultaneously temporary storage device can come back to life or consciousness, provides necessary experimental apparatus for experiment and scientific research cell come back to life or consciousness.

Although the embodiments of the present invention and the accompanying drawings are disclosed for illustrative purposes, those skilled in the art will appreciate that: various substitutions, changes and modifications are possible without departing from the spirit and scope of the present invention and the appended claims, and therefore, the scope of the present invention is not limited to the disclosure of the embodiments and the accompanying drawings.

Claims

1. The utility model provides a portable cryopreserved cell resuscitation box which characterized in that: comprises a base, a pot body frame arranged on the base, a screw rod frame arranged on the base, a water bath pot arranged on the pot body frame and a circulating water mechanism arranged on the left side of the water bath pot; the water bath includes the pot body, sets up at the insulation cover of the external side of pot, sets up in the constant temperature heating ring and the pot lid of coaxial arrangement in pot body upper end of bottom in the pot body, be provided with the lead screw on the upper portion crossbeam of lead screw frame, be equipped with servo motor on the lead screw admittedly, servo motor lower extreme coaxial arrangement pivot, in the pivot extension inserts the water bath, the pivot tip is equipped with one and freezes a tub recovery basket admittedly, it reciprocates to freeze tub recovery basket lead screw drive servo motor, and the servo motor drive freezes deposits tub recovery basket at the water bath internal rotation.

2. The portable cryopreserved cell resuscitation case of claim 1, wherein: the circulating water mechanism comprises a drain pipe communicated with the bottom of the pot body, a water inlet pipe communicated with the upper part of the pot body and a water pump connected with the drain pipe and the water inlet pipe, and a water inlet of the drain pipe is positioned below the constant-temperature heating ring.

3. The portable cryopreserved cell resuscitation case of claim 2, wherein: the circulating water mechanism also comprises a water replenishing pipe communicated with the water discharging pipe, and the water replenishing pipe, the water discharging pipe and the water inlet pipe are all provided with electromagnetism.

4. The portable cryopreserved cell resuscitation case of claim 1, wherein: the pipe recovery basket of depositing by freezing includes recovery basket body and fixed picture peg, the side from last equipartition interval system of recovery basket body has a plurality of inlet openings, this internal lower part of recovery basket is provided with fixed picture peg, recovery basket body upper surface equipartition interval system has a plurality of circular freezing to deposit a tub recess and deposits the hole, freezing to deposit a tub recess and deposit downthehole inlaying and adorn the freezing pipe of depositing, it deposits still to have the freezing of tub bottom of tubes portion to be used for fixed freezing to deposit to correspond to freeze to deposit on the fixed picture peg of tub recess position and deposit the hole.

5. The portable cryopreserved cell resuscitation case of claim 1, wherein: and a detachable thermometer and a temperature sensor are arranged in the water bath kettle.

6. The portable cryopreserved cell resuscitation case of claim 1, wherein: still be provided with control panel on the lead screw frame, the last display screen that installs of control panel, control panel respectively with servo motor, water pump, constant temperature heating ring and temperature sensor link to each other.

7. The portable cryopreserved cell resuscitation case of claim 1, wherein: and an elastic rubber ring is arranged on the lower end surface of the outer edge of the pot cover.