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CN212199205U - Photobioreactor for culturing algae - Google Patents

Photobioreactor for culturing algae Download PDF

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Publication number
CN212199205U
CN212199205U CN202020209482.2U CN202020209482U CN212199205U CN 212199205 U CN212199205 U CN 212199205U CN 202020209482 U CN202020209482 U CN 202020209482U CN 212199205 U CN212199205 U CN 212199205U
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tank
culture
cache
algae
photobioreactor
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CN202020209482.2U
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涂桂洪
邓惠平
王明日
谭志康
潘忠林
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Guangzhou Jinyinyuan Biotechnology Co ltd
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Guangzhou Jinyinyuan Biotechnology Co ltd
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Abstract

The utility model discloses a photobioreactor for culturing algae, each culture unit comprises an illumination culture tank and cache tanks connected with the illumination culture tank, each cache tank is arranged in sequence, adjacent cache tanks are communicated with each other to form a closed circulating system, the pollution of external microorganisms to culture liquid is avoided, and each culture unit is mutually independent, the step culture of algae is realized, and different illumination culture tanks can receive different illumination intensities, thereby being capable of fully adapting to the growth characteristics of algae in different stages; simultaneously, mutual independence between constant temperature equipment, air feed mechanism and each cultivation unit, it is more convenient to manage, is more convenient for realize extensive cultivation, and this utility model is used for bioengineering technical field.

Description

Photobioreactor for culturing algae
Technical Field
The utility model relates to the technical field of bioengineering, especially relate to a cultivate photobioreactor of alga.
Background
The existing photobioreactor for algae cultivation is generally in the form of an open raceway pond or a closed glass pipeline, but the cultivation of algae by using the open raceway pond is difficult to adjust the cultivation conditions of growth temperature, illumination and the like of algae, is easy to pollute, has unstable products, adopts an artificial light source when cultivating algae in a laboratory, has small production scale and high production cost, and is not suitable for large-scale production of algae products. Although the pollution problem is solved for the culture of algae by using the photobioreactor with the closed glass pipeline, the reactor cannot adjust the illumination intensity and temperature, the growth of the algae is greatly influenced, and the production period is prolonged.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to solve one of the technical problem that exists among the prior art at least, provide a be convenient for cultivate the photobioreactor of the cultivation alga of management on a large scale.
The photobioreactor for cultivating algae comprises a constant temperature device, a water tank, a heating device arranged on the water tank and a thermometer arranged in the water tank, wherein the water level in the water tank is not lower than two thirds of the height of the water tank; the culture units of each group respectively comprise an illumination culture tank and cache tanks connected with the illumination culture tank, algae and culture solution for algae culture are hermetically contained in each cache tank, each cache tank is arranged in a water tank, the bottom of each cache tank is immersed in water, and the cache tanks are sequentially arranged and adjacent cache tanks are communicated with each other; the air supply mechanism comprises an air supply main pipe and a plurality of air inlet branch pipes communicated with the air supply main pipe, the number of the air inlet branch pipes is the same as that of the culture units, and each air inlet branch pipe penetrates into a cache tank respectively.
According to the utility model discloses cultivate photobioreactor of alga has following beneficial effect at least: according to the photobioreactor for culturing algae, each culture unit comprises an illumination culture tank and cache tanks connected with the illumination culture tank, the cache tanks are sequentially arranged, adjacent cache tanks are mutually communicated to form a closed circulating system, pollution of external microorganisms to a culture solution is avoided, the culture units are mutually independent, the algae are cultured in a grading manner, and different illumination culture tanks can receive different illumination intensities, so that the growth characteristics of the algae in different stages can be fully adapted; meanwhile, the constant temperature device, the air supply mechanism and each culture unit are mutually independent, so that the management is more convenient and faster, and the large-scale culture is more conveniently realized.
According to the utility model discloses a some embodiments, each an air distributor, each are connected respectively to intake branch's end the bottom of buffer tank is arranged in to the air distributor equipartition.
According to some embodiments of the utility model, each illumination culture tank is including the cultivation upper tank and the cultivation lower tank of range upon range of setting respectively, cultivate the upper tank and cultivate to be connected through an at least communicating pipe between the lower tank.
According to some embodiments of the utility model, a feed liquor pipe, drain pipe are connected respectively between buffer tank and the cultivation upper tank, the cultivation lower tank, dispose the delivery pump on the drain pipe, the directional groove of cultivateing down of export of delivery pump.
According to some embodiments of the utility model, install the three-way valve on the feed liquor pipe, the three-way valve is the flow divider, the entry of flow divider with cultivate the upper trough switch-on and one of them directional buffer memory jar of export, another export of flow divider and the entrance connection of a discharging pipe, the exit end of discharging pipe stretches into in the adjacent buffer memory jar.
According to some embodiments of the present invention, each of the illumination culture tank and the buffer tank is made of a transparent material.
According to some embodiments of the utility model, each illumination culture tank top-down is range upon range of to be arranged, distributes from bottom to top illumination culture tank is connected with the buffer memory jar one-to-one that switches on in proper order from beginning to end.
According to some embodiments of the present invention, a water cooler is disposed beside the water tank; the import of cold water machine is connected to the lateral wall of water tank through outlet pipe, and the import is connected through the bottom of oral siphon and water tank.
According to some embodiments of the invention, the sterilizing filter is mounted at the inlet of the gas supply main.
According to some embodiments of the utility model, each the top of buffer memory jar sets up at least one exhaust hole respectively.
Drawings
The present invention will be further explained with reference to the accompanying drawings:
FIG. 1 is a schematic view of the overall structure of a photobioreactor for culturing algae according to an embodiment of the present invention;
FIG. 2 is a schematic structural view of a culture unit according to an embodiment of the present invention.
Detailed Description
This section will describe in detail the embodiments of the present invention, preferred embodiments of the present invention are shown in the attached drawings, which are used to supplement the description of the text part of the specification with figures, so that one can intuitively and vividly understand each technical feature and the whole technical solution of the present invention, but they cannot be understood as the limitation of the protection scope of the present invention.
In the description of the present invention, it should be understood that the orientation or positional relationship indicated with respect to the orientation description, such as up, down, front, rear, left, right, etc., is based on the orientation or positional relationship shown in the drawings, and is only for convenience of description and simplification of description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
In the description of the present invention, a plurality of means are one or more, a plurality of means are two or more, and the terms greater than, less than, exceeding, etc. are understood as not including the number, and the terms greater than, less than, within, etc. are understood as including the number. If the first and second are described for the purpose of distinguishing technical features, they are not to be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated or implicitly indicating the precedence of the technical features indicated.
In the description of the present invention, unless there is an explicit limitation, the words such as setting, installation, connection, etc. should be understood in a broad sense, and those skilled in the art can reasonably determine the specific meanings of the above words in combination with the specific contents of the technical solution.
Referring to fig. 1 and 2, a photobioreactor for culturing algae according to an embodiment of the present invention includes a thermostat device 1, a gas supply mechanism 2, and three sets of culture units. It should be noted that the number of culture units can be adjusted according to different situations, and is not limited to three sets in this embodiment.
Specifically, the thermostatic device 1 comprises a water tank 11, a heating device 12 arranged on the water tank 11 and a thermometer 13 arranged in the water tank 11, wherein the water level in the water tank 11 is not lower than two thirds of the height of the water tank 11; each group of culture units respectively comprises an illumination culture tank 31 and cache tanks 32 connected with the illumination culture tank 31, algae and culture solution for algae culture are hermetically contained in each cache tank 32, each cache tank 32 is arranged in the water tank 11, the bottom of each cache tank 32 is immersed in water, and further, each cache tank 32 is sequentially arranged and adjacent cache tanks 32 are communicated with each other; the air supply mechanism 2 comprises an air supply main pipe 21 and a plurality of air inlet branch pipes 22 communicated with the air supply main pipe 21, the number of the air inlet branch pipes 22 is the same as that of the culture units, and each air inlet branch pipe 22 penetrates into a cache tank 32.
According to the utility model discloses a photobioreactor for cultivating algae, it possesses constant temperature equipment 1, air feed mechanism 2 and three group culture units, each culture unit includes illumination culture tank 31, the buffer memory jar 32 of being connected with illumination culture tank 31, each buffer memory jar 32 arranges in proper order and communicates each other between the adjacent buffer memory jar 32, form closed circulation system, avoid external microorganism to cause the pollution to the culture solution, and each culture unit is independent each other, realized the stage culture to algae, and different illumination culture tanks 31 can accept different illumination intensity, thereby can fully adapt to the growth characteristic of algae in different stages; meanwhile, the constant temperature device 1, the air supply mechanism 2 and each culture unit are mutually independent, so that the management is more convenient and faster, and the large-scale culture is more conveniently realized.
Specifically, as shown in fig. 1, the buffer tanks 32 located at the initial position are connected to a feeding pipe 4, the feeding pipe 4 is connected to an external culture solution supplementing device, further, an air vent 321 is respectively disposed at the top of each buffer tank 32, and the air provided by the air supply mechanism 2 provides carbon dioxide for the algae in the buffer tanks 32, so that the oxygen generated by the photosynthesis performed by the algae can be exhausted from the air vent 321. Furthermore, a water cooler 14 is arranged beside the water tank 11; the inlet of the water cooler 14 is connected to the side wall of the water tank 11 through a water outlet pipe, and the inlet is connected to the bottom of the water tank 11 through a water inlet pipe. Furthermore, the thermometer 13, the water cooler 14 and the heating device 12 are in signal connection with a controller, the thermometer 13 measures the water temperature in the water tank 11 and feeds back the measurement structure to the controller, and the controller receives the signal and then controls the water cooler 14 and the heating device 12 to work, so that the water temperature in the water tank 11 is kept constant, and the normal survival of algae is guaranteed. More specifically, the heating device 12 in the present embodiment is a heating coil provided at the bottom of the water tank 11.
In some embodiments of the present invention, the end of each intake branch 22 is connected to an air distributor 23, and each air distributor 23 is disposed at the bottom of the buffer tank 32; specifically, referring to FIG. 1, the air distributor 23 employs a perforated collar. So set up for the air that gets into in the buffer tank 32 can be dispersed rapidly to make the distribution of air in buffer tank 32 more even, realize the high efficiency of air feed, provide sufficient carbon dioxide for the growth of algae, thereby improve the growth rate of algae.
In some embodiments of the present invention, each illumination cultivation tank 31 includes an upper cultivation tank 311 and a lower cultivation tank 312, which are stacked, and the upper cultivation tank 311 and the lower cultivation tank 312 are connected through at least one connection pipe 313. Meanwhile, a liquid inlet pipe 33 and a liquid outlet pipe 34 are respectively connected between the buffer tank 32 and the upper culture tank 311 and the lower culture tank 312, a delivery pump 341 is configured on the liquid outlet pipe, and the outlet of the delivery pump 341 points to the lower culture tank 312; further, in order to ensure that the culture solution is not contaminated and to improve the efficiency and stability of the transfer, a peristaltic pump is used as the transfer pump 341 in the embodiment. More specifically, three-way valves 331 are respectively installed on each liquid inlet pipe, each three-way valve 331 is a flow dividing valve, an inlet of the flow dividing valve is communicated with the culture upper tank 311, one outlet of the flow dividing valve points to the buffer tank 32, the other outlet of the flow dividing valve is connected with an inlet end of a discharge pipe 5, and an outlet end of the discharge pipe 5 extends into the adjacent buffer tank 32. The discharging pipe 5 is utilized to realize the effective connection of the adjacent buffer tanks 32, so that the relatively independent culture units are linked, the continuous culture of algae is realized, and the production period can be effectively shortened.
In some embodiments of the present invention, each illumination cultivation tank 31 is made of transparent material to ensure that light such as sunlight can smoothly enter the illumination cultivation tank 31, so that the algae can smoothly perform photosynthesis. In some embodiments, the material of each light culture tank 31 is plexiglass. More specifically, the light culture tanks 31 are arranged in a stacked manner from top to bottom, and the light culture tanks 31 arranged from bottom to top are connected with the buffer tanks 32 which are sequentially connected from beginning to end in a one-to-one correspondence manner. The light culture tanks 31 stacked from top to bottom form a photoreactor, and when the photoreactor is placed under the sun, sunlight sequentially passes through the culture solution in each light culture tank 31 from the top, and the light intensity of each light culture tank 31 is also sequentially weakened, so that the photoreactor can be better adapted to the biological characteristics of algae in different growth stages.
In some embodiments of the utility model, in order to guarantee the purity of the air that gets into in the cultivation unit, avoid external microorganism to cause the pollution to culture solution etc., install bacteria removal filter 24 in the entrance of air supply header pipe.
When the photobioreactor provided by the embodiment of the utility model is used for culturing haematococcus pluvialis to synthesize astaxanthin, the culture solution temperature in the water tank 11 and the buffer tank 32 is maintained at 22-27 ℃, the three groups of culture units are arranged to divide the culture of the haematococcus pluvialis into three stages, the first stage is a primary culture stage, the inoculation amount of the haematococcus pluvialis is 3% -10%, the required illumination intensity is only 1200-2000 Lux because the algae is in a delayed stage and the culture solution is clear and transparent, so the haematococcus pluvialis in the first stage is cultured in the culture unit of the illumination culture tank 31 positioned at the bottom layer; the second stage is a second stage culture stage, the haematococcus pluvialis is in a logarithmic growth phase, and as the density of algae in the culture solution is higher, the culture solution is green, the illumination intensity in the culture tank is weakened, and the illumination intensity is required to be 2000-5000 Lux, the three-way valve 331 is adjusted, and the haematococcus pluvialis is conveyed to the middle cache tank 32 through the discharge pipe 5, so that the haematococcus pluvialis can enter the illumination culture tank 31 in the middle layer for photosynthesis; the third stage is a stress stage, in which the algae is in a stable stage, and the haematococcus pluvialis needs to meet a large amount of astaxanthin under a stress environment with higher light intensity, so that the haematococcus pluvialis is conveyed to the culture unit of the light culture tank 31 positioned at the upper layer in the third stage.
The haematococcus pluvialis in each stage can enter the next stage after being cultured for 5-20 days. The algae in the third stage has synthesized a large amount of astaxanthin, and haematococcus pluvialis can be discharged and collected from the discharge pipe 5 by adjusting the three-way valve 341, and can be processed in the next step. Utilize the utility model discloses photobioreactor cultivates haematococcus pluvialis, has realized the semi-continuous cultivation to haematococcus pluvialis, only needs simple operation can get into next cultivation stage with haematococcus pluvialis, and production cycle shortens greatly.
Naturally, the invention is not limited to the above-described embodiments, and those skilled in the art can make equivalent modifications or substitutions without departing from the spirit of the invention, and such equivalent modifications or substitutions are included in the scope of the claims of the present application.

Claims (10)

1. A photobioreactor for culturing algae, comprising:
the constant temperature device comprises a water tank, a heating device arranged on the water tank and a thermometer arranged in the water tank, wherein the water level in the water tank is not lower than two thirds of the height of the water tank;
the culture units of each group respectively comprise an illumination culture tank and cache tanks connected with the illumination culture tank, algae and culture solution for algae culture are hermetically contained in each cache tank, each cache tank is arranged in a water tank, the bottom of each cache tank is immersed in water, and the cache tanks are sequentially arranged and adjacent cache tanks are communicated with each other;
the air supply mechanism comprises an air supply main pipe and a plurality of air inlet branch pipes communicated with the air supply main pipe, the number of the air inlet branch pipes is the same as that of the culture units, and each air inlet branch pipe penetrates into a cache tank respectively.
2. The photobioreactor for cultivating algae of claim 1, wherein: the tail end of each air inlet branch pipe is respectively connected with an air distributor, and each air distributor is uniformly distributed at the bottom of the cache tank.
3. The photobioreactor for cultivating algae of claim 1, wherein: each illumination culture tank comprises an upper culture tank and a lower culture tank which are arranged in a stacked mode, and the upper culture tank and the lower culture tank are connected through at least one communicating pipe.
4. The photobioreactor for cultivating algae according to claim 3, wherein: the buffer tank is connected a feed liquor pipe, drain pipe respectively with cultivate the upper trough, cultivate between the lower trough, dispose the delivery pump on the drain pipe, the groove is down cultivated to the delivery pump export orientation.
5. The photobioreactor for cultivating algae according to claim 4, wherein: the feed liquor is last to install the three-way valve, the three-way valve is the flow divider, the entry and the cultivation upper tank switch-on of flow divider and the directional buffer memory jar of one of them export, another export of flow divider is connected with the entrance point of a discharging pipe, the exit end of discharging pipe stretches into in the adjacent buffer memory jar.
6. The photobioreactor for cultivating algae of claim 1, wherein: each illumination culture tank and each cache tank are made of transparent materials.
7. The photobioreactor for cultivating algae of claim 6, wherein: the illumination culture tanks are arranged from top to bottom in a stacked mode, and the illumination culture tanks distributed from bottom to top are connected with the cache tanks which are sequentially communicated from beginning to end in a one-to-one correspondence mode.
8. The photobioreactor for cultivating algae of claim 1, wherein: a water cooler is arranged beside the water tank; the import of cold water machine is connected to the lateral wall of water tank through outlet pipe, and the import is connected through the bottom of oral siphon and water tank.
9. The photobioreactor for cultivating algae of claim 1, wherein: and a sterilizing filter is installed at the inlet of the air supply main pipe.
10. The photobioreactor for cultivating algae of claim 1, wherein: the top of each cache tank is provided with at least one exhaust hole.
CN202020209482.2U 2020-02-25 2020-02-25 Photobioreactor for culturing algae Active CN212199205U (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111154624A (en) * 2020-02-25 2020-05-15 广州市金因源生物技术有限公司 Photobioreactor for culturing algae

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111154624A (en) * 2020-02-25 2020-05-15 广州市金因源生物技术有限公司 Photobioreactor for culturing algae

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