CN211739631U - Protein sample storage device - Google Patents

Abstract

The utility model discloses a protein sample save set belongs to the refrigeration plant field. Comprises a box body, wherein the box body comprises a vacuum working chamber, a storage chamber and a freezing working chamber from top to bottom; a plurality of vacuum branch pipes are arranged in the vacuum working chamber; each vacuum branch pipe is provided with an electric control valve; the storage room is provided with a plurality of storage cabinets, and the storage trays are arranged in the storage cabinets; the preservation cabinet is provided with a vertical downward cornice plate; the storage disc is arranged in the storage cabinet in a drawing way; the push-pull front end of the object placing disc is provided with a push-pull panel, and the contact surface of the push-pull panel and the cornice plate is provided with a sealing strip. The device can effectively solve the problems of easy oxidation and denaturation of protein through vacuum and freezing, and ensures the stability of the protein reagent. Through setting for a plurality of independent vacuum's the cabinet of preserving in this device, the convenience of taking, to other reagent step influences, and the convenience of taking in corresponding single cabinet of preserving, and quick, be difficult to make mistakes.

Description

Protein sample storage device

Technical Field

The utility model relates to a refrigeration plant field, the device that protein sample was preserved during concretely relates to protein purification.

Background

Protein purification uses the inherent similarity and difference between different proteins, uses the similarity between various proteins to remove contamination of non-protein substances, and uses the difference between proteins to purify a target protein from other proteins. The size, shape, charge, hydrophobicity, solubility and biological activity of each protein can vary, and the protein can be extracted from a mixture such as E.coli lysate to obtain recombinant protein. In any case, care must be taken to maintain its stability, preserve its activity, and operate as much as possible on ice or in a refrigerated storage. Therefore, the preservation of the purified form also requires the preservation in a refrigerator. However, the existing refrigerating chamber is generally a door, and after the door is opened, patterns in the refrigerating chamber are all exposed, so that the stability of protein is influenced by opening and closing the door. Meanwhile, the refrigerating chamber is easy to be infected with microorganisms, which causes protein deterioration. The contact with oxygen during the preservation process can cause the oxidative deterioration of the reagent. If the reagent is stored in a freezing way by isolating air, the reagent can be ensured not to deteriorate for a long time. The preservation of some other tamper evident reagents is also a significant problem, as the reagents tend to deteriorate after tampering. This requires a more low temperature, oxygen-free environment for storage.

SUMMERY OF THE UTILITY MODEL

In order to solve the problem, the utility model provides an independently preserve and take, do not influence the protein sample save set of the stability of other reagents.

In order to achieve the above purpose, the present invention is realized by the following technical solutions:

a protein sample preservation device comprises a box body, wherein the box body comprises a vacuum working chamber, a preservation chamber and a freezing working chamber from top to bottom; a vacuum pump is arranged in the vacuum working chamber and connected with a vacuum main pipe, and the vacuum main pipe is connected with a plurality of vacuum branch pipes; each vacuum branch pipe is provided with an electric control valve; a plurality of preservation cabinets are arranged in the preservation room, and storage trays are arranged in the preservation cabinets; the preservation cabinet is provided with a vertical downward cornice plate; the storage disc is arranged in the storage cabinet in a drawing mode; the push-pull front end of the object placing disc is provided with a push-pull panel, and the push-pull panel is arranged below the cornice; the contact surface of the push-pull panel and the cornice plate is provided with a sealing strip; a vacuum pipe orifice is fixedly arranged at the top of the preservation cabinet; the vacuum pipe orifices are connected with the vacuum branch pipes in a one-to-one correspondence manner; a freezing device is arranged in the freezing working chamber and comprises an air compressor, a liquid nitrogen device, an air inlet and a cold air outlet; the storage chamber is provided with a cold air inlet which is connected with the cold air outlet; an air outlet is arranged in the storage chamber and is in butt joint with the air inlet; be equipped with discharge valve, manometer and temperature monitor on the cornice.

Furthermore, the box body is provided with an exhaust port, and the exhaust port is connected with the vacuum pump through a pipeline.

Further, the sealing strip is a vacuum chuck circular sealing strip.

Furthermore, a master controller is arranged on the side face of the box body, and a sub-controller is arranged on each cornice; the master controller and the slave controllers are in driving connection with the vacuum pump; and the master controller is in driving connection with all the electric control valves.

Further, an air pressure sensor and a photosensitive sensor are arranged in the storage cabinet.

Furthermore, each sub-controller is respectively connected with an air pressure sensor and a photosensitive sensor on the corresponding cornice plate; the electric control valve on the vacuum branch pipe on the storage cabinet corresponding to each cornice plate is in driving connection with the corresponding branch controller.

Furthermore, a handle is arranged on the push-pull panel.

The utility model discloses protein sample save set, its beneficial effect lies in:

(1) the device can effectively solve the problems of easy oxidation and denaturation of protein through vacuum and freezing, and ensures the stability of the protein reagent.

(2) Through setting for a plurality of independent vacuum's the cabinet of preserving in this device, the convenience of taking, to other reagent step influences, and the convenience of taking in corresponding single cabinet of preserving, and quick, be difficult to make mistakes.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without inventive exercise.

Fig. 1 is a schematic structural diagram of the present invention;

FIG. 2 is an expanded view of the storage cabinet;

1, a box body, 2, a vacuum working chamber, 3, a storage chamber and 4, a freezing working chamber; 5, an exhaust port, 6 a master controller and 7 branch controllers;

201 vacuum pump, 202 vacuum main pipe, 203 vacuum branch pipe and 204 electric control valve;

301 preservation cabinet, 302 storage tray, 303 cornice plate, 304 push-pull panel, 305 sealing strip and 306 cold air inlet; 307 air outlet; 308 exhaust valve, 309 pressure gauge, 310 temperature display; 311 a handle;

401 air compressor, 402 liquid nitrogen device, 403 air inlet, 404 cold air outlet.

Detailed Description

The present invention will be described in further detail with reference to the following embodiments and accompanying drawings.

A protein sample preservation device is shown in figures 1 and 2 and comprises a box body 1, wherein the box body 1 comprises a vacuum working chamber 2, a preservation chamber 3 and a freezing working chamber 4 from top to bottom; a vacuum pump 201 is arranged in the vacuum working chamber 2, the vacuum pump 201 is connected with a vacuum main pipe 202, and the vacuum main pipe 202 is connected with a plurality of vacuum branch pipes 203; each vacuum branch pipe 203 is provided with an electric control valve 204; an exhaust port 5 is arranged on the box body 1, and the exhaust port 5 is connected with a vacuum pump 201 through a pipeline; the exhaust port 5 is mainly used for exhaust in vacuum pumping; a plurality of preservation cabinets 301 are arranged in the preservation room 3, and storage trays 302 are arranged in the preservation cabinets 301; the preservation cabinet 301 is provided with a vertical downward cornice plate 303; the storage tray 302 is arranged in the storage cabinet 301 in a drawing mode, a push-pull panel 304 is arranged at the push-pull front end of the storage tray 302, and the push-pull panel 304 is arranged below the cornice 303; a handle 311 is arranged on the push-pull panel 304; the contact surface of the push-pull panel 304 and the cornice 303 is provided with a sealing strip 305; the sealing strip 305 is a vacuum cup circular sealing strip 305; a vacuum pipe orifice is fixedly arranged at the top of the preservation cabinet 301; the vacuum pipe orifices are correspondingly connected with the vacuum branch pipes 203 one by one; a freezing device is arranged in the freezing working chamber 4 and comprises an air compressor 401, a liquid nitrogen device 402, an air inlet 403 and a cold air outlet 404; the storage chamber 3 is provided with a cold air inlet 306, and the cold air inlet 306 is connected with the cold air outlet 404; an air outlet 307 is arranged in the storage chamber 3, and the air outlet 307 is in butt joint with the air inlet 403; the eaves plate 303 is provided with an exhaust valve 308, a pressure gauge 309 and a temperature display 310. A main controller 6 is arranged on the side surface of the box body 1, and a sub-controller 7 is arranged on each cornice 303; the master controller 6 and the slave controllers 7 are in driving connection with the vacuum pump 201. The master controller 6 is in driving connection with all the electric control valves 204; an air pressure sensor and a photosensitive sensor are arranged in the preservation cabinet 301, and each sub-controller 7 is respectively connected with the corresponding air pressure sensor and the photosensitive sensor on the cornice plate 303; the electric control valve 204 on the vacuum branch pipe 203 on the storage cabinet 301 corresponding to each cornice 303 is in driving connection with the corresponding branch controller 7.

The side wall of the preservation cabinet 301 is made of stainless steel with good heat transfer coefficient; the air compressor 401 in the freezing chamber 4 compresses air through the air inlet 403, inputs the compressed air into the liquid nitrogen device 402, cools the compressed air into the cold air through the liquid nitrogen device 402, and the cold air enters the storage chamber 3 through the cold air outlet 404 and the cold air inlet 306, so that the storage chamber 3 is cooled, and the cold air fills the space between the storage cabinets 301, so that the interior of the storage cabinet 301 is kept at a low temperature. The vacuum pump 201 evacuates the inside of the storage cabinet 301 through the vacuum branch pipe 203 and the vacuum main pipe 202. The preservation cabinet 301 is kept in a vacuum state, and the stability of the protein sample is effectively kept. When the user needs to take the storage cabinet, the corresponding exhaust valve 308 of the storage cabinet 301 is opened, and the push-pull panel 304 is pulled to take the storage cabinet after the pressure gauge 309 is normal; after the collection is finished, the push-pull panel 304 is closed, no light exists in the preservation cabinet 301, the photosensitive sensor senses no light and transmits a signal to the sub-controller 7, the sub-controller 7 controls the electric control valve 204 of the corresponding preservation cabinet 301 to be opened, and meanwhile, the vacuum pump 201 is started to vacuumize, so that the preservation cabinet 301 is restored to a vacuum state; when the air pressure sensor detects a vacuum state, the signal is transmitted to the sub-controller 7, and the sub-controller 7 controls the electric control valve 204 of the corresponding preservation cabinet 301 to be closed, so that the vacuum preservation is performed. Vacuum pump 201 in this device can be a plurality of, and different preservation cabinets 301 are managed and controlled respectively to a plurality of vacuum pumps 201. The vacuum pump 201 and the refrigerating device in the device are conventional devices. The controller in the present invention is a conventional controller, which mainly performs signal receiving and driving control, and such a controller is common knowledge and commercially available, and the structure, principle, model, etc. thereof are not described herein again.

The technical solutions provided by the embodiments of the present invention are described in detail above, and the principles and embodiments of the present invention are explained herein by using specific examples, and the descriptions of the above embodiments are only applicable to help understand the principles of the embodiments of the present invention; meanwhile, for a person skilled in the art, according to the embodiments of the present invention, there may be variations in the specific implementation manners and application ranges, and in summary, the content of the description should not be construed as a limitation to the present invention.

Claims (7)

1. A protein sample storage device characterized in that: the refrigerator comprises a box body, wherein the box body comprises a vacuum working chamber, a storage chamber and a freezing working chamber from top to bottom; a vacuum pump is arranged in the vacuum working chamber and connected with a vacuum main pipe, and the vacuum main pipe is connected with a plurality of vacuum branch pipes; each vacuum branch pipe is provided with an electric control valve; a plurality of preservation cabinets are arranged in the preservation room, and storage trays are arranged in the preservation cabinets; the preservation cabinet is provided with a vertical downward cornice plate; the storage disc is arranged in the storage cabinet in a drawing mode; the push-pull front end of the object placing disc is provided with a push-pull panel, and the push-pull panel is arranged below the cornice; the contact surface of the push-pull panel and the cornice plate is provided with a sealing strip; a vacuum pipe orifice is fixedly arranged at the top of the preservation cabinet; the vacuum pipe orifices are connected with the vacuum branch pipes in a one-to-one correspondence manner; a freezing device is arranged in the freezing working chamber and comprises an air compressor, a liquid nitrogen device, an air inlet and a cold air outlet; the storage chamber is provided with a cold air inlet which is connected with the cold air outlet; an air outlet is arranged in the storage chamber and is in butt joint with the air inlet; be equipped with discharge valve, manometer and temperature monitor on the cornice.

2. The protein sample storage device according to claim 1, wherein: the box body is provided with an exhaust port, and the exhaust port is connected with the vacuum pump through a pipeline.

3. The protein sample storage device according to claim 2, wherein: the sealing strip is a vacuum chuck circular sealing strip.

4. The protein sample storage device according to claim 1, wherein: a master controller is arranged on the side face of the box body, and a sub-controller is arranged on each cornice; the master controller and the slave controllers are in driving connection with the vacuum pump; and the master controller is in driving connection with all the electric control valves.

5. The protein sample storage device according to claim 1, wherein: an air pressure sensor and a photosensitive sensor are arranged in the preservation cabinet.

6. The protein sample storage device according to claim 4, wherein: each sub-controller is respectively connected with an air pressure sensor and a photosensitive sensor on the corresponding cornice; the electric control valve on the vacuum branch pipe on the storage cabinet corresponding to each cornice plate is in driving connection with the corresponding branch controller.

7. The protein sample storage device according to claim 1, wherein: the push-pull panel is provided with a handle.

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