CN210656940U

CN210656940U - Sterilization and subpackage integrated culture medium preparation device

Info

Publication number: CN210656940U
Application number: CN201920800022.4U
Authority: CN
Inventors: 王培�; 段海升; 聂晖; 宁春华
Original assignee: Jinan Baibo Biotechnology Co ltd
Current assignee: Jinan Baibo Biotechnology Co ltd
Priority date: 2019-05-30
Filing date: 2019-05-30
Publication date: 2020-06-02
Anticipated expiration: 2029-05-30

Abstract

The utility model relates to the technical field of medical equipment, in particular to a sterilization and split charging integrated culture medium preparation device, which comprises an outer barrel, an inner barrel and a material barrel, wherein an isolation layer is arranged outside the inner barrel; the outer barrel is matched with an outer cover, filling equipment is arranged on the outer cover, a material sucking pipe of the filling equipment extends into the material barrel, a material discharging pipe of the filling equipment is sleeved on a split charging pipe, the split charging pipe is installed on the outer cover in a penetrating mode, and an additive adding device is further arranged on the outer cover. The utility model reduces the pollution in the middle process, greatly reduces the probability of bacteria contamination, and has better sterilization effect; the waste is reduced, the loss of manpower is reduced, the waste of materials is reduced, and the production efficiency is also improved.

Description

Sterilization and subpackage integrated culture medium preparation device

Technical Field

The utility model relates to the technical field of medical equipment, in particular to sterilization partial shipment culture medium preparation ware as an organic whole.

Background

The culture medium is a mixed nutrient for providing nutrient substances for the non-growth and reproduction of microorganisms and plant activities, and is prepared from different nutrient substances. In the culture medium production process, often sterilization and partial shipment are separately gone on, just so increased the probability of contracting fungus, and when partial shipment to the barrel bottom portion, often the partial shipment is incomplete, has wasted the resource, has caused economic loss.

Disclosure of Invention

The utility model provides a make up the defect of prior art, provide a sterilization partial shipment culture medium preparation ware as an organic whole that high-efficient sterilization, partial shipment are thorough, simple and convenient swift.

The utility model discloses a realize through following technical scheme:

a sterilization and split charging integrated culture medium preparation device comprises a PLC control system and is characterized by further comprising an outer barrel, an inner barrel and a material barrel which are sequentially sleeved together from outside to inside, wherein the outer barrel and the inner barrel are fixedly connected together, the inner barrel and the material barrel are connected in a sliding mode, a hollow isolating layer is arranged outside the inner barrel, a pressurizing device and a cooling device are arranged between the outer barrel and the inner barrel, a heating and heat-insulating control device and a gravity sensor are arranged at the bottom of the inner barrel, a lifting device is arranged at the bottom of the outer barrel, and the lifting device is located on the diameter far away from the circle center; a magnetic stirrer is arranged at the bottom of the material barrel; the outer barrel is matched with an outer cover, filling equipment is arranged on the outer cover, a material sucking pipe of the filling equipment extends into the material barrel, a material discharging pipe of the filling equipment is sleeved on a subpackaging pipe, the subpackaging pipe is installed on the outer cover in a penetrating mode, and an additive adding device is further arranged on the outer cover.

The cooling device comprises: the air outlet pipe is arranged on the upper portion of the isolation layer, the condensation pipe is arranged at the lower end of the isolation layer, the air outlet pipe extends out of the outer barrel, an air blower is arranged at the lower end of the isolation layer, an air inlet pipe of the air blower extends out of the outer barrel, the air blower is connected with the condensation pipe, and the air outlet pipe and the air inlet pipe are both provided with filter membranes.

The supercharging equipment comprises a supercharger, a supercharging inlet pipe of the supercharger extends out of the outer barrel, a supercharging outlet pipe of the supercharger extends into the inner barrel, and filter membranes are arranged on the supercharging inlet pipe and the supercharging outlet pipe; the booster circuit is connected with the air pressure sensor.

The heating and heat-preserving control equipment is respectively connected with a temperature detection controller and a time controller which are arranged on the outer cover through circuits, and the temperature and the time are displayed through a display on the outer cover.

The additive adding device comprises an adding pipe, an additive inlet pipe and an additive outlet pipe, wherein the additive inlet pipe and the additive outlet pipe are both made of iron, the adding pipe is made of hydrophobic materials, and the subpackaging pipe and the additive inlet pipe are respectively in threaded through connection on the outer cover.

The subpackaging pipe and the additive inlet pipe are respectively in threaded connection with a subpackaging cover and an adding cover.

And a clamping groove and a clamping rail which are matched with each other are arranged between the material barrel and the inner barrel, and the clamping groove is arranged in the material barrel.

The material barrel is provided with a handle.

And a sealing ring is arranged between the outer barrel and the outer cover.

And the bottom of the outer barrel is provided with a roller.

The utility model has the advantages that:

the utility model is provided with the additive adding channel, the additive is convenient to add, the sterilization and the split charging are carried out in the same container, the pollution in the middle process is reduced, the probability of bacteria contamination is greatly reduced, the heating and heat preservation control equipment and the supercharging equipment are arranged to heat, boost and preserve heat, and the sterilization effect is more excellent; the magnetic stirrer works all the time in the processes of sterilization, cooling, heat preservation and split charging, so that the materials are kept uniform all the time; the lifting equipment makes the whole device incline when the materials are not much, and the materials flow towards the material suction pipe, so that the materials are subpackaged thoroughly, the waste is reduced, the loss of manpower is reduced, the waste of the materials is reduced, and the production efficiency is also improved.

Drawings

The present invention will be further described with reference to the accompanying drawings.

FIG. 1 is a schematic sectional view of the present invention;

fig. 2 is a schematic view of the connection of the inner barrel and the material barrel.

In the figure, 1 outer barrel, 2 inner barrel, 3 material barrel, 4 outer cover, 5 rollers, 6 gaps, 7 lifting equipment, 8 gravity sensor, 9 magnetic stirrer, 10 supercharger, 11 supercharging inlet pipe, 12 supercharging outlet pipe, 13 air pressure sensor, 14 display, 15 isolation layer, 16 air outlet pipe, 17 condensation pipe, 18 blower, 19 air inlet pipe, 20 heating and heat preservation control equipment, 21 temperature monitoring controller, 22 time controller, 23 clamping groove, 24 clamping rail, 25 clamping spring, 26 handle, 27 filling equipment, 28 suction pipe, 29 discharge pipe, 30 split charging pipe, 31 split charging cover, 32 adding pipe, 33 additive inlet pipe, 34 additive outlet pipe, 35 adding cover and 36 filter membrane.

Detailed Description

The attached drawings are specific embodiments of the invention. As shown in figures 1 to 2, the sterilization and subpackage integrated culture medium preparation device is carried out under a PLC control system, and comprises an outer barrel 1, the outer barrel 1 is matched with an outer cover 4 and is connected in a hinged mode, the outer barrel 1 and the outer cover 4 are sealed through a sealing ring, then an automatic lock is matched, just like a lock of a draw-bar box, but other modes such as a lock catch, a buckle and the like can be adopted, as long as the lock can be locked, a roller 5 such as a universal wheel is arranged at the bottom of the outer barrel 1, at least three rollers are arranged, four rollers are preferably used for facilitating pushing for safety, the outer barrel 1 is cylindrical, an inner barrel 2 is fixed in the outer barrel 1, the inner barrel 2 is also cylindrical, a relatively large gap 6 is reserved between the inner barrel 2 and the outer barrel 1, a lifting device 7 is arranged on the bottom radius (far away from the circle center) of the outer barrel 1, and the lifting device 7 is, for example, a fork shearing machine, a jack and the like are only small in model, a base of the lifting device 7 is installed on the bottom surface of the outer barrel 1, the telescopic part faces downwards, when the lifting device is not used, the telescopic part is suspended and extends out to support the ground when the lifting device is used, then the top of the outer barrel 1 is inclined by a counterforce, the inclination amplitude is not too large, namely 5-10 degrees, a gravity sensor 8 and a magnetic stirrer 9 are installed at the bottom of the inner barrel 2, and the gravity sensor 8 sends signals to the lifting device 7 through a PLC control system; a pressurizing device is arranged in a gap 6 between an inner barrel 2 and an outer barrel 1, the pressurizing device comprises a pressurizer 10 capable of controlling the upper limit of pressure, a pressurizing inlet pipe 11 of the pressurizer 10 extends out of the outer barrel 1, a pressurizing outlet pipe 12 of the pressurizer 10 extends upwards to the inner barrel 2, filter membranes 36 are arranged at the end parts of the pressurizing inlet pipe 11 and the pressurizing outlet pipe 12 to filter impurities in air, the pressurizer 10 is electrically connected with an air pressure sensor 13, the air pressure sensor 13 is arranged on an outer cover 4, the air pressure sensor 13 is connected to a display 14, and the display 14 is also arranged on the outer cover 4.

The outer cladding of interior bucket 2 has round isolation layer 15, and is hollow, and an air-out pipe 16 of isolation layer 15 upper portion installation leads to outside bucket 1 outside, at an air-out pipe 16 outer port department installation filter membrane 36, at an isolation layer 15 lower extreme installation condenser pipe 17, and the air-blower 18 is connected to the condenser pipe 17, and the air-supply line 19 of air-blower 18 stretches out from outer bucket 1, and a filter membrane 36 is installed to the outer port of air-supply line 19.

The bottom of the inner barrel 2 is also provided with a heating and heat-preserving control device 20, the heating and heat-preserving control device 20 belongs to the prior art, such as an electric cooker, and generally comprises a heating element and a controller, and details are not repeated, the heating and heat-preserving control device 20 is electrically connected with a temperature monitoring controller 21 and a time controller 22, the temperature monitoring controller 21 and the time controller 22 are both arranged on the outer cover 4, and the temperature and the time are also displayed through the display 14 on the outer cover 4.

A material barrel 3 is arranged in the inner barrel 2, the inner barrel 2 and the material barrel 3 are connected in a sliding mode through a clamping groove 23 and a clamping rail 24, the clamping rail 24 is inserted into the clamping groove 23, the inner barrel 2 and the material barrel 3 cannot rotate relatively, the material barrel 3 can only be lifted upwards, and the material barrel 3 can be taken out of the inner barrel 2; a clamp spring 25 is arranged in the material barrel 3, the clamp spring 25 and the lifting device 7 are positioned at two sides of the circle center of the outer barrel 1, and a handle 26 is arranged on the material barrel 3.

Installation filling equipment 27 in enclosing cover 4, can control filling volume and speed, filling equipment 27 inhale material pipe 28 and stretch into 3 interior cards of material bucket on jump ring 25, jump ring 25 can install several more, filling equipment 27's discharging pipe 29 overlaps on a partial shipment pipe 30, partial shipment pipe 30 external screw thread, screw up enclosing cover 4 through the external screw thread, partial outsider 4 that stretches out of partial shipment pipe 30, supporting partial shipment lid 31, inhale material pipe 28 and discharging pipe 29 and all be the plastic tubing that can high temperature and high pressure sterilization, inhale material pipe 28 and can stretch to 3 bottoms of material bucket.

The additive adding device is also arranged in the outer cover 4 and comprises an additive inlet pipe 33 and an additive outlet pipe 34 which are arranged at two ends of the additive adding pipe 32 and the additive outlet pipe 32, the additive inlet pipe 33 and the additive outlet pipe 34 are both made of iron pipes, the additive adding pipe 32 is made of hydrophobic materials, the additive inlet pipe 33 is provided with external threads and is screwed on the outer cover 4 through the external threads, one part of the additive inlet pipe 33 extends out of the outer cover 4, and then an adding cover 35 is matched.

The working process is as follows:

1 taking the preparation of nutrient agar culture medium as an example: the utility model is pushed to a sterilization room, a material barrel 3 is placed in an inner barrel 2 and fixed through a clamping groove 23 and a clamping rail 24, ingredients are weighed, quantitative purified water is added into the space between the material barrel 3 and the inner barrel 2, half of the configured culture water is added into the material barrel 3, a culture medium is added without chatting, then the rest water is added, after a magnetic stirrer 9 is opened and stirred for 30 minutes (the magnetic stirrer 9 works all the time in the following process until the filling is finished), an outer cover 4 is covered, an automatic lock is tightly locked, a split charging cover 31 and an adding cover 35 are screwed, parameter data such as sterilization temperature, sterilization time, heat preservation temperature, split charging temperature, rest weight, highest air pressure and the like are set on a display 14, then a heating and heat preservation control device 20 and a pressurizing device are started to sterilize, the heating and heat preservation control device 20 and the pressurizing device rapidly heat and pressurize, after the temperature is set, the timing begins, until the sterilization is accomplished, supercharging equipment stop work, air-blower 18 begins work, cooling, air-supply line 19 filters and carries out the air condensation through air-blower 18 behind the dust particle in the air, through condenser pipe 17, the aseptic air after the condensation is let in to isolation layer 15 to filter membrane 36 on the condensation mouth, then discharge through air-out pipe 16, microorganism or dust particle in the air enter into isolation layer 15 when filter membrane 36 on the air-out pipe 16 plays to prevent the backward flow, when dropping to the settlement heat preservation temperature, heating heat preservation controlgear 20 begins to keep warm, wait for the partial shipment.

Will the utility model discloses pushing away the partial shipment workshop, sheatheing the filling pipeline of sterilization on partial shipment pipe 30 in the disinfection environment, adjusting filling volume and filling speed, carrying out the partial shipment, when the partial shipment is to 3 bottoms surplus quantitative materials of material bucket, reach gravity sensor 8's setting value this moment, gravity sensor 8 gives PLC control system transmission signal, and PLC control system provides the signal for lifting equipment 7 again, and lifting equipment 7 starts, will the utility model discloses one side is raised, makes it slope, and the material pipe 28 departments are inhaled to the material flow direction to it is clean to divide the surplus material partial shipment.

Taking the preparation of the saprola medium added with chloramphenicol as an example, the steps from the beginning to the waiting of subpackaging are the same as the preparation of the nutrient agar medium, when subpackaging is carried out, the adding cover 35 is unscrewed under the sterile environment, the sterile filter is inserted, the chloramphenicol solution is sucked by the syringe and injected into the sterile filter for filtration and sterilization, the chloramphenicol solution enters the material barrel 3 along the additive inlet pipe 33, the adding pipe 32 and the additive outlet pipe 34, and then the filling is started, and the filling step is the same as the preparation of the nutrient agar medium.

Other technical features than those described in the specification are known to those skilled in the art.

Claims

1. A sterilization and split charging integrated culture medium preparation device comprises a PLC control system and is characterized by further comprising an outer barrel, an inner barrel and a material barrel which are sequentially sleeved together from outside to inside, wherein the outer barrel and the inner barrel are fixedly connected together, the inner barrel and the material barrel are connected in a sliding mode, a hollow isolating layer is arranged outside the inner barrel, a pressurizing device and a cooling device are arranged between the outer barrel and the inner barrel, a heating and heat-insulating control device and a gravity sensor are arranged at the bottom of the inner barrel, a lifting device is arranged at the bottom of the outer barrel, and the lifting device is located on the diameter far away from the circle center; a magnetic stirrer is arranged at the bottom of the material barrel; the outer barrel is matched with an outer cover, filling equipment is arranged on the outer cover, a material sucking pipe of the filling equipment extends into the material barrel, a material discharging pipe of the filling equipment is sleeved on a subpackaging pipe, the subpackaging pipe is installed on the outer cover in a penetrating mode, and an additive adding device is further arranged on the outer cover.

2. The sterilized and packaged integrated medium preparation apparatus as set forth in claim 1, wherein the cooling device comprises: the air outlet pipe is arranged on the upper portion of the isolation layer, the condensation pipe is arranged at the lower end of the isolation layer, the air outlet pipe extends out of the outer barrel, an air blower is arranged at the lower end of the isolation layer, an air inlet pipe of the air blower extends out of the outer barrel, the air blower is connected with the condensation pipe, and the air outlet pipe and the air inlet pipe are both provided with filter membranes.

3. The integrated sterilizing and packaging culture medium preparation device as claimed in claim 1, wherein the pressurizing device comprises a pressurizer, a pressurizing inlet pipe of the pressurizer extends out of the outer barrel, a pressurizing outlet pipe of the pressurizer extends into the inner barrel, and the pressurizing inlet pipe and the pressurizing outlet pipe are both provided with filter membranes; the booster circuit is connected with the air pressure sensor.

4. The sterilized and packaged integrated apparatus as set forth in claim 1, wherein the heating and keeping-warm control means is electrically connected to a temperature monitoring controller 21 and a time controller mounted on the outer cover, respectively, and the temperature and time are displayed by a display on the outer cover.

5. The integrated sterilizing and dispensing culture medium preparation device as claimed in claim 1, wherein the additive adding device comprises an additive inlet pipe and an additive outlet pipe which are arranged at two ends of the additive adding pipe, the additive inlet pipe and the additive outlet pipe are both made of iron, the additive adding pipe is made of hydrophobic materials, and the dispensing pipe and the additive inlet pipe are respectively in threaded through connection with the outer cover.

6. The sterilized and packaged integrated medium preparation apparatus as set forth in claim 5, wherein the packaging tube and the additive inlet tube are respectively screw-coupled with a packaging cap and a filling cap.

7. The integrated sterilizing and packaging culture medium preparation device according to claim 1, wherein a clamping groove and a clamping rail are arranged between the material barrel and the inner barrel, and the clamping groove is arranged in the material barrel.

8. The integrated sterile and packaged culture medium preparation device according to claim 7, wherein the material barrel is provided with a handle.

9. The sterilized and packaged integrated culture medium preparation apparatus as claimed in claim 1, wherein a sealing ring is provided between the outer tub and the outer cover.

10. The sterilized and packaged integrated culture medium preparation device as claimed in claim 1, wherein the bottom of the outer barrel is provided with a roller.