CN210237649U - Stem cell culture robot - Google Patents
Stem cell culture robot Download PDFInfo
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- CN210237649U CN210237649U CN201920798505.5U CN201920798505U CN210237649U CN 210237649 U CN210237649 U CN 210237649U CN 201920798505 U CN201920798505 U CN 201920798505U CN 210237649 U CN210237649 U CN 210237649U
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Abstract
A stem cell culture robot, comprising: the shell is internally provided with a cavity; the tissue pretreatment device is arranged in the containing cavity and is used for pretreating biological tissues; the main operation platform is arranged in the cavity, the main operation platform is connected with the tissue pretreatment device, and the biological tissue pretreated by the tissue pretreatment device is transmitted to the main operation platform for culture. The utility model provides a stem cell culture robot can carry out the preliminary treatment back to biological tissue through setting up at the tissue preprocessing device that holds the intracavity, handles in order to obtain the stem cell through the biological tissue after main operation platform to the preliminary treatment and cultivates for the operation to the stem cell becomes more convenient and swift, can prevent external pollution to biological tissue moreover, has improved the cultivation qualification rate of stem cell.
Description
Technical Field
The utility model belongs to the medical equipment field specifically discloses a stem cell culture robot.
Background
Stem cells are a type of pluripotent cells that are capable of self-replication and under certain conditions can differentiate into cells of one or more tissues or organs. Stem cells can be divided into embryonic stem cells and adult stem cells, and are generally round or oval, small in cell volume, but large in cell nucleus, mostly euchromatin, and high in telomerase activity.
In the process of culturing stem cells, in order to ensure good culturing effect of the cells, the cells need to be uniformly dispersed in a culture solution, and the cells need to be fully contacted with the culture solution so as to ensure the supply of nutrients required by the growth of the cells. In order to obtain stem cells, it is necessary to perform operations, cell separation, and the like on biological tissues, and in the operation process, the device is easily contaminated, which is inconvenient.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a stem cell culture robot to in operation acquire stem cell to biological tissue and cultivate.
Therefore, the utility model provides a stem cell culture robot, include:
the shell is internally provided with a cavity;
the tissue pretreatment device is arranged in the containing cavity and is used for pretreating biological tissues;
the main operation platform is arranged in the cavity, the main operation platform is connected with the tissue pretreatment device, and the biological tissue pretreated by the tissue pretreatment device is transmitted to the main operation platform for culture.
Preferably, the pretreatment device comprises:
the first liquid preparation center is used for distributing the aminase material;
a tissue preprocessor connected with the first liquid preparation center, wherein after the biological tissue is stored in the tissue preprocessor, the amine enzyme material is configured in the tissue preprocessor for preprocessing;
and the first waste liquid processor is connected with the tissue preprocessor and is used for discharging waste liquid in the tissue preprocessor.
Preferably, the method further comprises the following steps:
the conveying belt is arranged on the top wall of the accommodating cavity;
and the mechanical arm is connected to the transmission belt and is used for shearing the biological tissue arranged in the tissue preprocessor when the transmission belt moves to the position of the tissue preprocessor.
Preferably, the end of the mechanical arm is further provided with a sampling and sample-adding device for at least one of sampling and sample-adding the tissue preprocessor and the main operation platform.
Preferably, a centrifuge is further included for centrifuging the biological tissue to facilitate processing of the biological tissue on the tissue pretreatment device or the main operation platform.
Preferably, the method further comprises the following steps:
the second liquid preparation center is connected with the main operating platform and is used for preparing liquid for the main operating platform;
and the second waste liquid processor is connected with the main operating platform and used for discharging the waste liquid in the main operating platform.
Preferably, the device further comprises an optical detector for observing biological tissues of the main operation center and a digital management platform connected with the optical detector and the conveyor belt for controlling the optical detector and the conveyor belt.
Preferably, the method further comprises the following steps:
the incubator is connected with the main operation center and is used for culturing the biological tissue processed by the main operation center;
the discharging bin is connected with the incubator and is used for taking the biological tissue out of the incubator;
and the freezing and storing pipe assembly is used for freezing and storing the biological tissue processed by the main operation center.
Preferably, the method further comprises the following steps:
the ultraviolet disinfection monitor is used for carrying out ultraviolet disinfection on the inside of the accommodating cavity;
the negative pressure laminar flow monitor is used for monitoring the negative pressure and the laminar flow in the containing cavity;
and the carbon dioxide joint is communicated with the containing cavity and is used for adding carbon dioxide into the containing cavity.
Preferably, the housing is made of a transparent material.
Compared with the prior art, the utility model provides a stem cell culture robot can carry out the preliminary treatment back to biological tissue through setting up at the tissue preprocessing device who holds the intracavity, handles in order to obtain the stem cell through the biological tissue after main operation platform to the preliminary treatment and cultivates for become more convenient and swift to the operation of stem cell, can prevent external pollution to biological tissue moreover, improved the cultivation qualification rate of stem cell.
Drawings
FIG. 1 is a schematic structural view of a stem cell culturing robot according to the present invention;
FIG. 2 is a schematic view of the structure in direction II-II of FIG. 1;
in the figure:
10-a housing; 11-carbon dioxide junction; 12-a conveying belt; 13-a robotic arm; 20-a digital management platform; 30-discharging bin; 31-an incubator; 32-freezing and storing the pipe assembly; 40-a main operating platform; 41-a second liquid preparation center; 42-a second waste liquid processor; 43-centrifuge; 50-an optical detector; 60-a tissue pretreatment device; 61-a first liquid preparation center; 62-a tissue pre-processor; 63-a first waste liquid processor; 70-ultraviolet disinfection monitor; 71-negative pressure laminar flow monitor.
Detailed Description
The present invention will be further explained with reference to the accompanying drawings.
Fig. 1 is a schematic structural view of a stem cell culturing robot according to the present invention, and fig. 2 is a schematic structural view in the direction II-II in fig. 1 (the top surface of a housing 10 is omitted). As shown in fig. 1 and 2, the stem cell culture robot comprises a housing 10, a tissue pretreatment device 60, a main operation platform 40, a conveyor belt 12 and a robot arm 13, an optical detector 50, a digital management platform 20, an incubator 31, a discharge bin 30, a cryopreservation tube assembly 32, an ultraviolet disinfection monitor 70, a negative pressure laminar flow monitor 71 and a carbon dioxide joint 11.
The housing 10 has a cavity therein, and the above components are located in the cavity or mounted on the sidewall of the cavity. In the present embodiment, the housing 10 is made of a transparent material, and the internal components and the operation thereof, as well as the culture of the biological tissue, can be seen from the outside.
The tissue pretreatment device 60 is disposed in the cavity of the housing 10 and is used for pretreating biological tissue, wherein the pretreatment includes operations such as crushing, disinfection and digestion. In this embodiment, the pretreatment device includes a first liquid preparation center 61, a tissue pretreatment device 62, and a first waste liquid treatment device 63. The first liquid preparation center 61 is used for dispensing an amine enzyme material, the tissue preprocessor 62 is connected with the first liquid preparation center 61, and the amine enzyme material is configured in the tissue preprocessor 62 for preprocessing after the biological tissue is stored in the tissue preprocessor 62; the first waste liquid processor 63 is connected to the tissue preprocessor 62, and is configured to discharge waste liquid in the tissue preprocessor 62. After entering the tissue pretreatment device 60, fresh tissue such as umbilical cord tissue is soaked in ethanol for sterilization, and then cut by a mechanical arm 13 on a conveyor belt 12 using a scissors or other devices to separate the desired tissue, and then digested by pancreatin or collagenase to be transferred to a main console.
The housing 10 is further provided with a transmission belt 12 and a mechanical arm 13, the transmission belt 12 is disposed on the top wall of the accommodating cavity, and the mechanical arm 13 is connected to the transmission belt 12 and is configured to shear the biological tissue disposed in the tissue pre-processor 62 when the transmission belt 12 moves to the position of the tissue pre-processor 62. In this embodiment, a sampling and sample-adding device is further disposed at an end of the robot arm 13, and is used for at least one of sampling and sample-adding the tissue pre-processor 62 and the main operation platform 40.
The main operation platform 40 is disposed in the cavity, the main operation platform 40 is connected to the tissue pretreatment device 60, and the biological tissue pretreated by the tissue pretreatment device 60 is transferred to the main operation platform 40 for culture.
In addition, the stem cell culture robot further comprises a second liquid preparation center 41 and a second waste processor, wherein the second liquid preparation center 41 is connected with the main operation platform 40 and is used for preparing liquid for the main operation platform 40. And the second waste liquid processor 42 is connected with the main operation platform 40 and is used for discharging waste liquid in the main operation platform 40. The main operation platform 40 is used for culture by adding culture medium, and different culture media are changed according to various requirements, or cytokines are added, and culture induction differentiation is carried out, for example, the culture solution is changed after three days of culture.
The centrifuge 43 is used to centrifuge the biological tissue for processing on the tissue pre-processing device 60 or the main operation platform 40. When the induction is finished, the cells can be directly conveyed out of the cell bottle according to the requirement, and then conveyed to a tissue pretreatment module for digestion to prepare cell suspension. The cell suspension can be taken out for use, or can be resuspended by using a freezing medium after centrifugation, and then transferred out for freezing. In addition, the centrifuge 43 needs to be used every time it digests.
The optical detector 50 is used to observe the biological tissue of the main operation center, and may be an optical microscope, a fluorescence microscope, or the like. The digital management platform 20 is connected to the optical detector 50 and the transport belt 12 for controlling the optical detector 50 and the transport belt 12. The culture medium can be transferred to a built-in optical detector 50 for observation during the culture of the main operation platform 40, and the automatic photographing time of the optical detector 50 (such as an optical microscope) can be set, such as 8-hour photographing once, 24-hour photographing and 48-hour photographing, so as to analyze the growth and differentiation of the culture medium. Preferably, the optical detector 50 can also be a fluorescence microscope, and an antibody label can be added on the platform of the main operation screen, and then the antibody label is transmitted to the optical detector 50 for analysis to see the molecular change.
The incubator 31 is connected to the main operation center, and is used for culturing the biological tissue processed by the main operation center. The discharging bin 30 is connected with the incubator 31, and is used for taking out the biological tissue from the incubator 31. The cryopreservation tube assembly 32 is used for cryopreserving the biological tissue processed by the main operation center.
In this embodiment, the cavity of the housing 10 may further include an ultraviolet disinfection monitor 70 and a negative pressure laminar flow monitor 71. The ultraviolet disinfection monitor 70 is used for ultraviolet disinfection of the cavity. And the negative pressure laminar flow monitor 71 is used for monitoring the negative pressure and the laminar flow in the cavity. In some embodiments, the housing 10 may further include a carbon dioxide connector 11, and the carbon dioxide connector 11 is communicated with the cavity and is used for adding carbon dioxide into the cavity.
The working process of the stem cell culture robot according to the above embodiment is described in detail below.
First, fresh tissue (for example, umbilical cord tissue) is placed in the tissue pre-processor 62, and after being soaked and sterilized in ethanol, the biological tissue is cut by the scissors of the robot arm 13 on the conveyor belt 12, and the desired tissue is separated. Then, the first liquid preparation center 61 prepares pancreatin or collagenase into the tissue preprocessor 62 to digest the biological tissue, and then transmits the digested biological tissue to the main operation platform 40.
Then, a culture medium is added to the main operation platform 40 through the second liquid preparation center 41 for culture, and different culture media are replaced according to various requirements, or cytokines are added, and culture induction differentiation is performed, for example, liquid replacement is performed after three days of culture. During the culture process, the biological tissue can be transmitted to the built-in optical detector 50 for observation, and the automatic photographing time of the optical detector 50 can be set, for example, 8 hours, 24 hours and 48 hours, so as to analyze the growth and differentiation of the biological tissue. In some embodiments, the optical detector 50 may be a fluorescence microscope, and the antibody label may be added to the main operation platform 40 and then transmitted to the fluorescence microscope for analysis to see the molecular change.
When induction is complete, the cells may be transferred directly, e.g., in a cell vial, ready for use, or transferred to a tissue pretreatment module for digestion to produce a cell suspension, as desired. The cell suspension can be taken out for use, or can be resuspended by using a freezing medium after being centrifuged by using a centrifuge 43 and then transferred out for freezing. Among them, in the digestion market, the centrifuge 43 is used for centrifugation.
During the above operation, the ultraviolet disinfection monitor 70 is required to perform ultraviolet disinfection on the chamber and the negative pressure laminar flow monitor 71 is required to monitor the negative pressure and laminar flow conditions in the chamber.
The stem cell culture robot provided by the embodiment can be used for pretreating biological tissues through the tissue pretreatment device 60 arranged in the containing cavity and culturing the biological tissues after pretreatment by processing the biological tissues after pretreatment through the main operation platform 40 to obtain stem cells, so that the operation on the stem cells is more convenient and quicker, the external pollution to the biological tissues can be prevented, and the culture qualification rate of the stem cells is improved.
It is to be understood that the present invention is not limited to the above-described embodiments, and various changes and modifications may be made without departing from the spirit and scope of the present invention, and it is intended to cover such changes and modifications if they fall within the scope of the claims and their equivalents.
Claims (10)
1. A stem cell culture robot, comprising:
the shell is internally provided with a cavity;
the tissue pretreatment device is arranged in the containing cavity and is used for pretreating biological tissues;
the main operation platform is arranged in the cavity, the main operation platform is connected with the tissue pretreatment device, and the biological tissue pretreated by the tissue pretreatment device is transmitted to the main operation platform for culture.
2. The stem cell culture robot of claim 1, wherein the pre-processing device comprises:
the first liquid preparation center is used for distributing the aminase material;
a tissue preprocessor connected with the first liquid preparation center, wherein after the biological tissue is stored in the tissue preprocessor, the amine enzyme material is configured in the tissue preprocessor for preprocessing;
and the first waste liquid processor is connected with the tissue preprocessor and is used for discharging waste liquid in the tissue preprocessor.
3. The stem cell culture robot of claim 2, further comprising:
the conveying belt is arranged on the top wall of the accommodating cavity;
and the mechanical arm is connected to the transmission belt and is used for shearing the biological tissue arranged in the tissue preprocessor when the transmission belt moves to the position of the tissue preprocessor.
4. The stem cell culture robot of claim 3, wherein the robotic arm further comprises a sample applicator at an end thereof for at least one of sampling and applying samples to the tissue pre-processor and the main operation platform.
5. The stem cell culture robot of claim 4, further comprising a centrifuge for centrifuging the biological tissue to facilitate processing of the biological tissue on the tissue pre-processing device or the main operation platform.
6. The stem cell culture robot of claim 5, further comprising:
the second liquid preparation center is connected with the main operating platform and is used for preparing liquid for the main operating platform;
and the second waste liquid processor is connected with the main operating platform and used for discharging the waste liquid in the main operating platform.
7. The stem cell culture robot of claim 6, further comprising an optical detector for observing biological tissue of the main operation center and a digital management platform connected to the optical detector and the transport belt for controlling the optical detector and the transport belt.
8. The stem cell culture robot of claim 7, further comprising:
the incubator is connected with the main operation center and is used for culturing the biological tissue processed by the main operation center;
the discharging bin is connected with the incubator and is used for taking the biological tissue out of the incubator;
and the freezing and storing pipe assembly is used for freezing and storing the biological tissue processed by the main operation center.
9. The stem cell culture robot of claim 8, further comprising:
the ultraviolet disinfection monitor is used for carrying out ultraviolet disinfection on the inside of the accommodating cavity;
the negative pressure laminar flow monitor is used for monitoring the negative pressure and the laminar flow in the containing cavity;
and the carbon dioxide joint is communicated with the containing cavity and is used for adding carbon dioxide into the containing cavity.
10. The stem cell culture robot of claim 8, wherein the housing is made of a transparent material.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201920798505.5U CN210237649U (en) | 2019-05-29 | 2019-05-29 | Stem cell culture robot |
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| CN201920798505.5U CN210237649U (en) | 2019-05-29 | 2019-05-29 | Stem cell culture robot |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110066733A (en) * | 2019-05-29 | 2019-07-30 | 广东唯泰生物科技有限公司 | Stem cell cultivates robot |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110066733A (en) * | 2019-05-29 | 2019-07-30 | 广东唯泰生物科技有限公司 | Stem cell cultivates robot |
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