CN214206629U - Automatic screening and optimizing device for optical imaging assisted edible mushroom breeding - Google Patents
Automatic screening and optimizing device for optical imaging assisted edible mushroom breeding Download PDFInfo
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- CN214206629U CN214206629U CN202022273567.XU CN202022273567U CN214206629U CN 214206629 U CN214206629 U CN 214206629U CN 202022273567 U CN202022273567 U CN 202022273567U CN 214206629 U CN214206629 U CN 214206629U
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Abstract
An optical imaging auxiliary automatic screening optimization device for edible fungus breeding belongs to the technical field of bioengineering breeding, and comprises an object stage, a main guide rail, an auxiliary guide rail and an imaging instrument fixer; when the device is used, firstly, edible strains are placed in a grid area of a table top of an objective table, after parameter setting and initialization are carried out on an upper computer, a main guide rail controls the transverse movement of an imaging instrument, a secondary guide rail controls the longitudinal movement of the imaging instrument, the imaging instrument acquires image information of the edible strains, the upper computer analyzes images to extract phenotypes, and a servo motor and the imaging instrument work in a set period. The invention reduces the manual supervision and intervention, and greatly improves the information acquisition efficiency and accuracy during the breeding and screening of the edible fungi.
Description
Technical Field
The utility model belongs to the technical field of bioengineering breeding, in particular to optics formation of image supplementary edible mushroom breeding automatic screening optimization device.
Background
The edible fungus breeding refers to the mating among different species or different strains in the species, and generally comprises the stages of hybridization pairing, pipe transfer propagation, preliminary screening, secondary screening, cultivation test, demonstration popularization and the like. In the primary screening and secondary screening stages, measurement and calculation are required: 1. the growth rate of hyphae; 2. phenotypic characteristics of the fruiting fungus block bag; 3. (ii) a phenotypic characteristic of a fruit body; 4. yield of fruit body; 5. suitable for growth temperature, humidity, etc. The measurement calculation methods commonly used at present include: the calipers measure the scale information of hypha, fungus block bag and sporocarp, and an optical imaging instrument is used manually to obtain the phenotype information of the shape, the water content and the like of the hypha. The optical imaging enhances the information richness of phenotype extraction, reduces the destructiveness to thalli, and is an effective assistant tool for breeding.
The objects of primary screening and secondary screening of edible fungus breeding are various hybrid varieties, a large amount of manual thallus information acquisition is needed, the efficiency of frequent manual determination is low for real-time detection, and entry registration errors are easily caused, so that the breeding progress and accuracy are influenced. Combine optical imaging means, design an automatic sieving mechanism of domestic fungus breeding, the phenotype information of regularly monitoring the thallus, replace artifical survey, avoid human error or error, can improve domestic fungus breeding efficiency and success rate.
SUMMERY OF THE UTILITY MODEL
In domestic fungus breeding screening stage, carry out automated monitoring screening function in order to realize the phenotype to the thallus of numerous and diverse varieties, the utility model provides an automatic screening optimization device of supplementary domestic fungus breeding of optical imaging, the device mainly comprises objective table and sliding guide, the objective table divides into impartial net and is used for placing each variety bacterial strain, sliding guide divide into leading rail and vice guide, leading rail is used for carrying on visual camera, multispectral camera, imaging instrument such as fluorescence camera, imaging instrument can lateral shifting on leading rail, leading rail can longitudinal movement on vice guide, in order to ensure that imaging instrument can cover each net of objective table. The specific technical scheme is as follows:
an optical imaging auxiliary automatic screening and optimizing device for edible fungus breeding comprises an object stage 1, a main guide rail 2, an auxiliary guide rail 3 and an imaging instrument fixer 4;
the objective table 1 consists of supporting legs 5 and a table top 6, the table top 6 is evenly divided into a plurality of grid areas, each grid area is used for cultivating an edible strain, and the grid areas are placed in a circular culture dish identification area 7 in the center of the grid;
the main body structure of the main guide rail 2 consists of a beam 8 and a support frame 9, the beam 8 and the support frame 9 are formed by connecting triangular fixing supports 10, a rubber guide rail bar 11 is respectively arranged at the upper part and the lower part in the beam 8, and two concave wheels 12 with servo motors are occluded between the two rubber guide rail bars 11;
the main structure of the auxiliary guide rail 3 is a beam 8, a rubber guide rail bar 11 is respectively arranged at the upper part and the lower part in the beam 8 of the auxiliary guide rail, and two concave wheels 12 with servo motors are occluded between the two rubber guide rail bars 11;
the main guide rail 2 and the auxiliary guide rail 3 are connected together through a triangular connector 13, the stability of the structure is ensured, and the axis of the concave wheel 12 is connected with the bottom edge vertex angle of the triangular bracket 13;
the imaging instrument holder 4 is connected with the axis of the concave wheel 12 through a connector 14, the lower end of the imaging instrument holder 4 comprises three imaging instrument fixing points 15 for fixing imaging instruments 16 (including a visible light camera, a multispectral camera and a fluorescence camera), and the fixing points 15 can rotate transversely to adjust the angle of the imaging instruments.
A servo motor on the main guide rail controls the concave wheel 12 to rotate, so that the imaging instrument 16 transversely moves above the table board 6; the servo motor on the secondary rail controls the rotation of the geneva wheel 12 to move the primary rail longitudinally, thereby moving the imaging apparatus 16 longitudinally over the tabletop 6.
Under the motion control of the main guide rail and the auxiliary guide rail, the imaging instrument 16 regularly shoots and images the edible strains of each grid on the table board 6 one by one, and transmits the images back to the upper computer for processing and analysis to obtain related phenotype information.
The upper computer records the coding state of the servo motor, determines a grid area corresponding to the edible strain detected by the current camera, and simultaneously the visible light camera collects image information and feeds the image information back to the servo motor control system by identifying the circular culture dish identification area to adjust the position so as to obtain the optimal observation point position.
The use method of the optical imaging assisted edible fungus breeding automatic screening optimization device comprises the following steps:
step 1:
placing each cultured edible strain culture dish in a circular culture dish identification area 7 in a grid area of a table board 6, and setting growth conditions such as temperature, humidity and the like;
step 2:
resetting an upper computer for controlling the servo motor, the imaging instrument and image analysis, setting the working period of the imaging system, executing initialization by the upper computer, and restoring the imaging instrument holder 4 to the initial position;
and step 3:
in a working period, the upper computer controls servo motors on the main guide rail 2 and the auxiliary guide rail 3, the imaging instrument fixer 4 is moved, an edible strain on the table top is traversed to shoot an image, wherein the multispectral camera works all day long, the visible light camera works in the daytime, the fluorescent camera works at night, the image is analyzed by the upper computer, phenotype information such as water content, component content and growth vigor of the edible strain is analyzed and obtained from the multispectral image, the phenotype information such as the form, scale and the like of the edible strain is analyzed and obtained from the visible light image and the fluorescent image, and various phenotype information is registered;
and 4, step 4:
after the growth period of the edible fungi is finished, the computer comprehensively analyzes the collected and registered phenotype information, and retains the edible strains of good varieties by combining the existing discrimination standard.
The utility model discloses an automatic screening optimization device of supplementary domestic fungus breeding of optical imaging compares with current domestic fungus breeding screening operation means, and beneficial effect is:
the main guide rail and the auxiliary guide rail realize the full coverage of the imaging instrument on the table grid area, the accurate control of the servo motor provides a foundation for the automatic operation of image acquisition, and the efficiency of phenotype information acquisition is greatly improved;
secondly, the positioning precision of the camera is further improved by combining image analysis and circular target detection of the upper computer;
thirdly, the ID of each grid area can be conveniently obtained by utilizing an encoder of the servo motor, so that the automatic arrangement of the data entries is realized;
the three different imaging instruments complement each other, more phenotype information is extracted, and data verification is carried out, so that the accuracy of information extraction is improved;
and fifthly, the object stage has longitudinal expandability so as to realize the edible fungus breeding experiment with larger sample scale.
Drawings
FIG. 1 is a schematic view of the overall structure of an optical imaging-assisted automatic screening and optimizing device for edible fungus breeding;
FIG. 2 is a schematic view of an objective table of the optical imaging assisted edible fungi breeding automatic screening optimization device of the present invention;
FIG. 3 is a schematic diagram of a main guide rail of the optical imaging assisted automatic screening and optimizing device for edible fungus breeding of the present invention;
FIG. 4 is a schematic view of an auxiliary guide rail of the optical imaging auxiliary automatic screening and optimizing device for edible fungus breeding of the present invention;
fig. 5 is the utility model discloses an imaging instrument fixer sketch map of supplementary domestic fungus breeding automated screening optimizing apparatus of optical imaging.
Wherein: 1-an objective table, 2-a main guide rail, 3-an auxiliary guide rail, 4-an imaging instrument fixer, 5-an objective table supporting foot, 6-an objective table top, 7-a circular culture dish identification area, 8-a cross beam, 9-a main guide rail supporting frame, 10-a triangular fixing support, 11-a rubber guide rail bar, 12-a concave wheel with a servo motor, 13-a triangular connector, 14-an imaging instrument connector, 15-an imaging instrument fixing point and 16-an imaging instrument.
Detailed Description
The present invention will be further described with reference to the following specific embodiments, but the present invention is not limited to these embodiments.
Example 1
An optical imaging auxiliary automatic screening and optimizing device for edible fungus breeding comprises an object stage 1, a main guide rail 2, an auxiliary guide rail 3 and an imaging instrument fixer 4;
the objective table 1 consists of supporting feet 5 and a table board 6, the table board 6 is evenly divided into 100 multiplied by 100 grid areas, each grid area is used for cultivating an edible strain, and a circular culture dish identification area 7 is arranged in the center of the grid;
the main structure of the main guide rail 2 consists of a beam 8 and a support frame 9, the beam 8 and the support frame 9 are connected by a triangular fixing support 10, a rubber guide rail bar 11 is respectively arranged at the upper part and the lower part in the beam 8, and two concave wheels 12 with servo motors are occluded between the two rubber guide rail bars 11;
the main structure of the auxiliary guide rail 3 is a beam 8, a rubber guide rail bar 11 is respectively arranged at the upper part and the lower part in the beam 8 of the auxiliary guide rail, and two concave wheels 12 with servo motors are occluded between the two rubber guide rail bars 11;
the main guide rail 2 and the auxiliary guide rail 3 are connected together through a triangular connector 13, the stability of the structure is ensured, and the axis of the concave wheel 12 is welded with the bottom edge vertex angle of the triangular bracket 13;
the imaging instrument holder 4 is connected with the axis of the concave wheel 12 through a connector 14, and the lower end of the imaging instrument holder 4 comprises three imaging instrument fixing points 15 for fixing imaging instruments 16 (including a visible light camera, a multispectral camera and a fluorescence camera).
The servo motor on the main guide rail controls the concave wheel 12 to rotate, so that the imaging instrument 16 can transversely monitor the table top 6.
The servomotor on the secondary rail controls the rotation of the concave wheel 12, so that the primary rail moves longitudinally, and the imaging instrument 16 monitors the tabletop 6 longitudinally.
Under the motion control of the main guide rail and the auxiliary guide rail, the imaging instrument 16 regularly shoots and images the edible strains of each grid on the table board 6 one by one, and transmits the images back to the upper computer for processing and analysis to obtain related phenotype information.
The upper computer records the coding state of the servo motor, determines a grid area corresponding to the edible strain detected by the current camera, and simultaneously the visible light camera collects image information and feeds the image information back to the servo motor control system by identifying the circular culture dish identification area to adjust the position so as to obtain the optimal observation point position.
A use method of an optical imaging assisted edible fungus breeding automatic screening optimization device comprises the following steps:
step 1:
placing each cultured edible strain culture dish in a circular culture dish identification area 7 in a grid area of a table board 6, and setting a group of growth environments: the temperature was 28 ℃ and the humidity was 80%.
Step 2:
resetting an upper computer for controlling the servo motor, the imaging instrument and image analysis, setting the working period of the imaging system to be 2 hours, executing initialization by the upper computer, and restoring the imaging instrument fixer 4 to an initial position;
and step 3: in a working period, the upper computer controls servo motors on the main guide rail 2 and the auxiliary guide rail 3, the imaging instrument fixer 4 is moved, an edible strain on a table top is traversed to shoot an image, wherein the multispectral camera works all day long, the visible light camera works in the daytime (8: 00-18: 00), the fluorescent camera works at night (18: 00-8: 00), the image is analyzed by the upper computer, phenotype information such as the water content, the component content and the growth vigor of the edible strain is analyzed and obtained from the multispectral image, the phenotype information such as the form and the scale of the edible strain is analyzed and obtained from the visible light image and the fluorescent image, and all the phenotype information is registered;
and 4, step 4:
after the growth period of the edible fungi is finished, the computer comprehensively analyzes the collected and registered phenotype information, and retains the edible strains of good varieties by combining the existing discrimination standard.
The utility model provides an automatic screening optimization device of supplementary domestic fungus breeding of optical imaging, during the device use, at first place the edible bacterial strain in the grid region of objective table mesa, carry out parameter setting and initialization back to the host computer, the lateral shifting of main guide rail control imaging instrument, the longitudinal movement of vice guide rail control imaging instrument, the image information of edible bacterial strain is gathered to the imaging instrument, carry out the analysis by the host computer to the image and draw the phenotype, servo motor and imaging instrument work in the settlement cycle. The utility model discloses reduce manpower and watch and intervene, greatly improve the collection efficiency and the degree of accuracy of information.
Claims (7)
1. An optical imaging auxiliary automatic edible fungus breeding screening optimization device is characterized by comprising an object stage (1), a main guide rail (2), an auxiliary guide rail (3) and an imaging instrument fixer (4);
the objective table (1) consists of supporting legs (5) and a table board (6), the table board (6) is divided into a plurality of grids, and the edible strains are placed in a circular culture dish identification area (7) in the middle of the grids;
the main body structure of the main guide rail (2) consists of a cross beam (8) and a support frame (9), and the cross beam (8) and the support frame (9) are connected by a triangular fixing support (10); a rubber guide rail bar (11) is respectively arranged at the upper part and the lower part in the beam (8), and a concave wheel (12) is positioned between the two rubber guide rail bars (11);
the main body structure of the auxiliary guide rail (3) is a cross beam (8), and the main guide rail (2) and the auxiliary guide rail (3) are connected together through a triangular connector (13);
the imaging instrument fixing device (4) is connected with the axis of the concave wheel (12) through a connector (14), the lower end of the imaging instrument fixing device (4) comprises three imaging instrument fixing points (15) for fixing an imaging instrument (16), and the imaging instrument (16) comprises a visible light camera, a multispectral camera and a fluorescence camera.
2. The automated screening and optimizing apparatus for edible fungi breeding assisted by optical imaging as claimed in claim 1, wherein the center of the grid is marked by a regular geometric figure for placing edible fungi.
3. The automated screening and optimizing device for edible fungus breeding assisted by optical imaging as claimed in claim 1, wherein the main guide rail (2) uses two concave wheels to ensure the stability of the imaging instrument holder.
4. The automated screening and optimizing device for edible fungus breeding assisted by optical imaging as claimed in claim 1, wherein the concave wheels (12) of the auxiliary guide rail (3) are connected with the support frame (9) of the main guide rail (2) by triangular connectors (13) to ensure the stability of the main guide rail (2).
5. The automatic screening and optimizing device for edible fungus breeding assisted by optical imaging as claimed in claim 1, wherein a servo motor on the main guide rail (2) controls the concave wheel (12) to rotate, so that the imaging instrument (16) moves transversely above the table top (6).
6. The automatic screening and optimizing device for edible fungus breeding assisted by optical imaging as claimed in claim 1, wherein a servo motor on the secondary guide rail (3) controls a concave wheel (12) to rotate, so that the primary guide rail moves longitudinally, and an imaging instrument (16) moves longitudinally above the table top (6).
7. The automated screening optimization device for edible fungus breeding assisted by optical imaging as claimed in claim 1, wherein the imaging instrument fixing point (15) can be laterally adjusted in angle to ensure alignment with the target.
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| CN112136599A (en) * | 2020-10-13 | 2020-12-29 | 嘉兴古奥基因科技有限公司 | Automatic screening and optimizing device for optical imaging assisted edible mushroom breeding |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112136599A (en) * | 2020-10-13 | 2020-12-29 | 嘉兴古奥基因科技有限公司 | Automatic screening and optimizing device for optical imaging assisted edible mushroom breeding |
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