CN203846026U - Device for collecting circulating tumor cell - Google Patents
Device for collecting circulating tumor cell Download PDFInfo
- Publication number
- CN203846026U CN203846026U CN201420258725.6U CN201420258725U CN203846026U CN 203846026 U CN203846026 U CN 203846026U CN 201420258725 U CN201420258725 U CN 201420258725U CN 203846026 U CN203846026 U CN 203846026U
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- CN
- China
- Prior art keywords
- strainer tube
- diameter
- circulating tumor
- tumor cell
- collection device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 title claims abstract description 22
- 239000012528 membrane Substances 0.000 claims description 28
- 230000004308 accommodation Effects 0.000 claims description 13
- 238000007789 sealing Methods 0.000 claims description 6
- 239000011148 porous material Substances 0.000 claims description 5
- 238000001914 filtration Methods 0.000 abstract description 11
- 210000004369 blood Anatomy 0.000 abstract description 7
- 239000008280 blood Substances 0.000 abstract description 7
- 208000002151 Pleural effusion Diseases 0.000 abstract 1
- 239000012530 fluid Substances 0.000 abstract 1
- 230000001926 lymphatic effect Effects 0.000 abstract 1
- 206010028980 Neoplasm Diseases 0.000 description 24
- 201000011510 cancer Diseases 0.000 description 20
- 210000004027 cell Anatomy 0.000 description 12
- 238000000605 extraction Methods 0.000 description 4
- 150000007523 nucleic acids Chemical class 0.000 description 4
- 102000039446 nucleic acids Human genes 0.000 description 4
- 108020004707 nucleic acids Proteins 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 239000006166 lysate Substances 0.000 description 3
- 210000000056 organ Anatomy 0.000 description 3
- 210000005259 peripheral blood Anatomy 0.000 description 3
- 239000011886 peripheral blood Substances 0.000 description 3
- 210000004881 tumor cell Anatomy 0.000 description 3
- 206010027476 Metastases Diseases 0.000 description 2
- 239000004677 Nylon Substances 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 210000001124 body fluid Anatomy 0.000 description 2
- 239000010839 body fluid Substances 0.000 description 2
- 210000005266 circulating tumour cell Anatomy 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 210000002751 lymph Anatomy 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 230000007170 pathology Effects 0.000 description 2
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 206010008342 Cervix carcinoma Diseases 0.000 description 1
- 208000005016 Intestinal Neoplasms Diseases 0.000 description 1
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 1
- 208000005718 Stomach Neoplasms Diseases 0.000 description 1
- 208000006105 Uterine Cervical Neoplasms Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 201000010881 cervical cancer Diseases 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 201000002313 intestinal cancer Diseases 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 231100000518 lethal Toxicity 0.000 description 1
- 230000001665 lethal effect Effects 0.000 description 1
- 201000007270 liver cancer Diseases 0.000 description 1
- 208000014018 liver neoplasm Diseases 0.000 description 1
- 201000005202 lung cancer Diseases 0.000 description 1
- 208000020816 lung neoplasm Diseases 0.000 description 1
- 210000001365 lymphatic vessel Anatomy 0.000 description 1
- 206010025482 malaise Diseases 0.000 description 1
- 230000009401 metastasis Effects 0.000 description 1
- 230000001394 metastastic effect Effects 0.000 description 1
- 206010061289 metastatic neoplasm Diseases 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 201000000498 stomach carcinoma Diseases 0.000 description 1
- 210000000115 thoracic cavity Anatomy 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 230000004614 tumor growth Effects 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a device for collecting circulating tumor cells. The device comprises a filtering tube, an auxiliary filtering tube and a casing, wherein the filtering tube comprises a tube body; the tube body is internally provided with horizontally arranged grids; a filtering film of which the average aperture is 6-10 mu m, and a film pressing ring for fixing the filtering film are arranged on the grids; the auxiliary filtering tube comprises a connecting part and a containing part of which the diameter is increased; the connecting part can be inserted into the upper end of the filtering tube; the inner diameter of the casing is greater than the outer diameter of the lower part of the filtering tube. By adopting the device, circulating tumor cells can be rapidly and effectively enriched in blood, lymphatic fluid or pleural effusion.
Description
Technical field
The present invention relates to biological technical field, relate to particularly a kind of collection device of circulating tumor cell.
Background technology
Cancer is one of main cause of the death causing mankind's death, the data presentation of the World Health Organization (WHO), and within 2008, global cancer new cases are about 1,270 ten thousand, and cancer mortality number of cases is 7,600,000.The annual newly-increased cancer patients in the whole world is about 9,000,000 people at present, and expecting the year two thousand twenty whole world cancer morbidity will be than present increase by 50%, and the annual newly-increased cancer patients's number in the whole world will reach 1,500 ten thousand people.Sickness rate the highest cancer in the whole world is lung cancer at present, is mammary cancer successively, is intestinal cancer, cancer of the stomach, liver cancer and cervical cancer.China is one of cancer morbidity and the highest country of mortality ratio in the world, and there is 20% new cancer patient in the whole world in China, has 1/4 cancer mortality patient in China.
The morbidity of cancer is some organ or tissue that comes from body, cancer start from single cancer cells, single cancer cells constantly divides, rises in value, breaks up and just can produce one little cancer cells and form tumour.In the time that tumor growth arrives certain volume, cancer cells will come off, and is diffused into other tissue or the organ of body by blood vessel or lymphatic vessel, and this can form cancer metastasis.It is the initial period of realizing metastases that cancer cells enters blood circulation, for the final metastatic lesion that forms tumour provides possibility.Circulating tumor cell (Circulating Tumor Cells, CTCs) refers to the tumour cell that enters human peripheral blood.By detecting the tumour cell generation that effectively predicting tumors shifts in peripheral blood.The lethal major cause of most cancer patients is that tumour cell obtains transfer ability, is diffused into other organ or tissues by blood.Therefore in blood, collect and detect circulating tumor cell and there is important clinical value.
As everyone knows, blood testing does not need relatively simple, the little wound of tumor tissues sample, technology, is convenient to repeatedly repeat same patient, is easy to be accepted by patient, has a prior using value for clinical.If can catch cancer cells from the body fluid such as peripheral blood or lymph liquid, just can solve clinical patients with terminal without pathology sample, or the few problem of pathology sample, can be clinician's diagnosis and treatment, chemotherapy prognosis provides the reference frame of Rapid Science.
But still there is no the device of the quick and convenient collection of energy CTC at present.
Utility model content
The purpose of this utility model is to provide a kind of collection device of circulating tumor cell, to solve the above-mentioned problems in the prior art.
The technical scheme that the utility model provides is as follows:
A collection device for circulating tumor cell, is characterized in that, comprising:
One strainer tube, described strainer tube comprises a body, is provided with horizontally disposed grid in body, grid is provided with the filter membrane that mean pore size is 6-10 μ m, and a press mold ring for fixing filter membrane;
One auxiliary chimney filter, this auxiliary chimney filter comprises the accommodation section of a junction and an enlarged-diameter, wherein this connection section can be plugged into the upper end of strainer tube; And
One sleeve pipe, this casing inner diameter is greater than the lower outer diameter of strainer tube.
In a preferred embodiment of the present utility model, described strainer tube comprises columnar body, and the bottom of body connects a pars infundibularis, and the top of body stretches out and establishes lid, the turning upper opening that covers body of this lid; At the intersection of pars infundibularis and body, inside is provided with horizontally disposed grid, and grid is provided with filter membrane, is provided with press mold ring on filter membrane.
In a preferred embodiment of the present utility model, described sleeve pipe comprises the body of bottom sealing, and the mediad of this bottom is provided with plunger; Wherein, the diameter of plunger is less than the internal diameter of the pars infundibularis shank of strainer tube, and casing inner diameter is greater than the external diameter of the body of strainer tube.
In a preferred embodiment of the present utility model, auxiliary chimney filter comprises the accommodation section of a junction and an enlarged-diameter, and wherein connection section is tubulose, and external diameter is less than the internal diameter of tube body of strainer tube.
In another preferred embodiment of the present utility model, described strainer tube comprises columnar body, and the upper end of this body is provided with internal thread, is provided with horizontally disposed grid in lower end, and grid is provided with filter membrane, is provided with press mold ring on filter membrane.
In another preferred embodiment of the present utility model, auxiliary chimney filter comprises the accommodation section of a junction and an enlarged-diameter, wherein the external diameter of connection section is slightly less than the internal diameter of tube body of strainer tube, its outer wall is provided with the outside screw corresponding with the internal thread of strainer tube, in addition, also establish a lid, this lid can seal the upper opening of accommodation section.
In another preferred embodiment of the present utility model, sleeve pipe is the body of a bottom sealing, and this bottom is flat, and casing inner diameter is greater than the external diameter of strainer tube body.
The beneficial effects of the utility model are: the present invention has set up a kind of collection device of circulating tumor cell,
1), be provided with filter membrane in strainer tube, can be at blood, enrichment circulating tumor cell rapidly and efficiently in the long-pending body fluid in lymph liquid or thoracic cavity;
2), simple to operate, can cover lid, centrifuging in whizzer, or strainer tube is received on a negative pressure device and filtered;
3), enrichment fast and easy, operating process is short;
4), auxiliary chimney filter and strainer tube coordinate, and can increase big filter volume;
5) after filtration enrichment, put sleeve pipe, can directly guarantee again to add the solution of strainer tube can soak into filter membrane.Now, the cell on filter membrane can be carried out to resuspension, also can directly add the nucleic acid of the extraction cells such as lysate.
Brief description of the drawings
Fig. 1 is structural representation and local amplification the thereof of the strainer tube of embodiment mono-;
Fig. 2 is the structural representation of the sleeve pipe of embodiment mono-;
Fig. 3 is the fit structure schematic diagram of embodiment mono-strainer tube and plunger tube;
Fig. 4 is the structural representation of embodiment mono-auxiliary chimney filter;
Fig. 5 is the strainer tube of embodiment mono-and the fit structure schematic diagram of auxiliary sleeve pipe;
Fig. 6 is structural representation and local amplification the thereof of the strainer tube of embodiment bis-;
Fig. 7 is the structural representation of embodiment bis-auxiliary chimney filters;
Fig. 8 is the structural representation of embodiment bis-bottom tubes;
Fig. 9 is the one-piece construction schematic diagram of embodiment bis-.
Embodiment
Embodiment mono-
Referring to figs. 1 through Fig. 5, the collection device of circulating tumor cell is made up of strainer tube 1, sleeve pipe 2, auxiliary chimney filter 3 etc.
Wherein, as shown in Figure 1, strainer tube 1 comprises columnar body 11, the about 25mm of its length, diameter 13mm, the bottom of body 11 connects a pars infundibularis 12, the top of body 11 stretches out and establishes lid 13, and the turning upper opening that covers body 11 of this lid 13 is so that follow-up nucleic acid extraction.At the intersection of pars infundibularis 12 and body 11, inside is provided with horizontally disposed grid 14, and grid 14 is provided with filter membrane 15, and the material of filter membrane can be PC or nylon, and the mean pore size of filter membrane is 6-10 μ m, more preferably 7-9 μ m.On filter membrane, be provided with press mold ring 16.
As shown in Figure 2, sleeve pipe 2 comprises the body 21 of bottom sealing, and the mediad of this bottom is provided with plunger 22.Wherein, the diameter of plunger 22 is slightly less than the internal diameter of pars infundibularis 12 shanks of strainer tube 1, and body 21 internal diameters are slightly larger than the external diameter of body 11.
As shown in Figure 4, auxiliary chimney filter 3 comprises the accommodation section 32 of a junction 31 and an enlarged-diameter, and wherein connection section 31 is tubulose, and external diameter is slightly less than body 11 internal diameters of strainer tube 1.The internal diameter of accommodation section 32 is about 40mm.
Use of the present utility model is as follows:
First will assist chimney filter 3 to be plugged on strainer tube 1, as shown in Figure 5, then the shank of the pars infundibularis of strainer tube 11 will be socketed to a negative pressure device, blood or solution will be injected to auxiliary chimney filter 3, and afterwards, open negative pressure device and filter.
After filtration finishes, take off auxiliary chimney filter 3, strainer tube 1 is set on sleeve pipe 2, now, sleeve pipe 2 and strainer tube 1 closely interlock, and guarantee again to add the solution of strainer tube 1 can soak into filter membrane.Now, the cell on filter membrane can be carried out to resuspension, also can directly add the nucleic acid of the extraction cells such as lysate.
Embodiment bis-
Referring to Fig. 6 to Fig. 9, the collection device of circulating tumor cell is made up of strainer tube 4, auxiliary chimney filter 5 and sleeve pipe 6 etc.
Wherein, as shown in Figure 6, strainer tube 4 comprises columnar body 41, the about 20mm of its length, diameter 13mm, the upper end of body 11 is provided with internal thread 42, in lower end, be provided with horizontally disposed grid 43, grid 43 is provided with filter membrane 44, and the material of filter membrane can be PC or nylon, the mean pore size 6-10 μ m of filter membrane, more preferably 7-9 μ m.On filter membrane, be provided with press mold ring 45.
As shown in Figure 7, auxiliary chimney filter 5 comprises the accommodation section 53 of a junction 51 and an enlarged-diameter, and wherein the external diameter of connection section 51 is slightly less than body 41 internal diameters of strainer tube 4, and its outer wall is provided with the outside screw 52 with internal thread 42 correspondences of strainer tube.The internal diameter of accommodation section 53 is about 26mm.In addition, also establish a lid 7, this lid 7 can seal the upper opening of accommodation section 53.
As shown in Figure 8, sleeve pipe 6 is the body of a bottom sealing, and this bottom is flat, and sleeve pipe 6 internal diameters are slightly larger than the external diameter of body 41.
Use of the present utility model is as follows:
First will assist chimney filter 5 spirals to be connected on strainer tube 4, blood or solution will be injected to auxiliary chimney filter 3, after covering lid 7, can pack in a 50ml centrifuge tube centrifugal.
After filtration finishes, take off auxiliary chimney filter 5, strainer tube 4 is inserted in sleeve pipe 6, now, sleeve pipe 6 and strainer tube 4 are combined closely, and guarantee again to add the solution of strainer tube 4 can soak into filter membrane.Now, the cell on filter membrane can be carried out to resuspension, also can directly add the nucleic acid of the extraction cells such as lysate.
Claims (8)
1. a collection device for circulating tumor cell, is characterized in that, comprising:
One strainer tube, described strainer tube comprises a body, is provided with horizontally disposed grid in body, grid is provided with the filter membrane that mean pore size is 6-10 μ m, and a press mold ring for fixing filter membrane;
One auxiliary chimney filter, this auxiliary chimney filter comprises the accommodation section of a junction and an enlarged-diameter, wherein this connection section can be plugged into the upper end of strainer tube; And
One sleeve pipe, this casing inner diameter is greater than the lower outer diameter of strainer tube.
2. the collection device of a kind of circulating tumor cell as claimed in claim 1, it is characterized in that: described strainer tube comprises columnar body, the bottom of body connects a pars infundibularis, and the top of body stretches out and establishes lid, the turning upper opening that covers body of this lid; At the intersection of pars infundibularis and body, inside is provided with horizontally disposed grid, and grid is provided with filter membrane, is provided with press mold ring on filter membrane.
3. the collection device of a kind of circulating tumor cell as claimed in claim 2, is characterized in that: described sleeve pipe comprises the body of bottom sealing, and the mediad of this bottom is provided with plunger; Wherein, the diameter of plunger is less than the internal diameter of the pars infundibularis shank of strainer tube, and casing inner diameter is greater than the external diameter of the body of strainer tube.
4. the collection device of a kind of circulating tumor cell as claimed in claim 2, is characterized in that: auxiliary chimney filter comprises the accommodation section of a junction and an enlarged-diameter, and wherein connection section is tubulose, and external diameter is less than the internal diameter of tube body of strainer tube.
5. the collection device of a kind of circulating tumor cell as claimed in claim 1, it is characterized in that: described strainer tube comprises columnar body, the upper end of this body is provided with internal thread, is provided with horizontally disposed grid in lower end, grid is provided with filter membrane, is provided with press mold ring on filter membrane.
6. the collection device of a kind of circulating tumor cell as claimed in claim 5, it is characterized in that: auxiliary chimney filter comprises the accommodation section of a junction and an enlarged-diameter, wherein the external diameter of connection section is less than the internal diameter of tube body of strainer tube, its outer wall is provided with the outside screw corresponding with the internal thread of strainer tube, in addition, also establish a lid, this lid can seal the upper opening of accommodation section.
7. the collection device of a kind of circulating tumor cell as claimed in claim 6, is characterized in that: sleeve pipe is the body of a bottom sealing, and this bottom is flat, and casing inner diameter is greater than the external diameter of strainer tube body.
8. the collection device of a kind of circulating tumor cell as described in claim 1 to 7 any one, is characterized in that: filter membrane mean pore size is 7-9 μ m.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420258725.6U CN203846026U (en) | 2014-05-20 | 2014-05-20 | Device for collecting circulating tumor cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420258725.6U CN203846026U (en) | 2014-05-20 | 2014-05-20 | Device for collecting circulating tumor cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203846026U true CN203846026U (en) | 2014-09-24 |
Family
ID=51559064
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420258725.6U Expired - Lifetime CN203846026U (en) | 2014-05-20 | 2014-05-20 | Device for collecting circulating tumor cell |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203846026U (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105510600A (en) * | 2016-01-28 | 2016-04-20 | 山东省药物研究院 | Method for detecting ER gene of peripheral blood circulating tumor cells of patient suffering from advanced breast cancer |
| CN105647784A (en) * | 2016-03-08 | 2016-06-08 | 闫维新 | Negative pressure based specific cell treatment device |
| CN107917835A (en) * | 2017-12-30 | 2018-04-17 | 广州阳普医疗科技股份有限公司 | A kind of blood-taking device for screening circulating tumor cell |
| CN107937259A (en) * | 2017-12-29 | 2018-04-20 | 南方医科大学南方医院 | A kind of portable membrane type cell harvestor |
| WO2019001228A1 (en) * | 2017-06-30 | 2019-01-03 | 开启基因股份有限公司 | Fixed tube of nucleic acid extraction component, and nucleic acid extraction component |
-
2014
- 2014-05-20 CN CN201420258725.6U patent/CN203846026U/en not_active Expired - Lifetime
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105510600A (en) * | 2016-01-28 | 2016-04-20 | 山东省药物研究院 | Method for detecting ER gene of peripheral blood circulating tumor cells of patient suffering from advanced breast cancer |
| CN105510600B (en) * | 2016-01-28 | 2018-03-30 | 山东省药物研究院 | The detection method of advanced breast cancer patient Peripheral Circulation tumour cell ER genes |
| CN105647784A (en) * | 2016-03-08 | 2016-06-08 | 闫维新 | Negative pressure based specific cell treatment device |
| WO2019001228A1 (en) * | 2017-06-30 | 2019-01-03 | 开启基因股份有限公司 | Fixed tube of nucleic acid extraction component, and nucleic acid extraction component |
| CN109207340A (en) * | 2017-06-30 | 2019-01-15 | 开启基因股份有限公司 | The fixing pipe and nucleic acid extraction component of nucleic acid extraction component |
| EP3647408A4 (en) * | 2017-06-30 | 2021-03-24 | Catchgene Co., Ltd. | Fixed tube of nucleic acid extraction component, and nucleic acid extraction component |
| US11365406B2 (en) | 2017-06-30 | 2022-06-21 | Catchgene Co., Ltd. | Fixed tube of nucleic acid extraction component, and nucleic acid extraction component |
| CN107937259A (en) * | 2017-12-29 | 2018-04-20 | 南方医科大学南方医院 | A kind of portable membrane type cell harvestor |
| CN107917835A (en) * | 2017-12-30 | 2018-04-17 | 广州阳普医疗科技股份有限公司 | A kind of blood-taking device for screening circulating tumor cell |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee | ||
| CP03 | Change of name, title or address |
Address after: 361000, No. 39, Ding Shan Road, Haicang District, Fujian, Xiamen Patentee after: AMOY DIAGNOSTICS Co.,Ltd. Address before: Haicang District of Xiamen City, Fujian province 361000 Xinyang Street Weng Kok Road No. 289, No. 4 Building 5 layer Branch Center Patentee before: Xiamen Aide Biomedical Technology Co.,Ltd. |
|
| CX01 | Expiry of patent term |
Granted publication date: 20140924 |