CN202078585U - Artificial liver bioreactor with U-shaped hollow fiber tubes - Google Patents
Artificial liver bioreactor with U-shaped hollow fiber tubes Download PDFInfo
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- CN202078585U CN202078585U CN2011201523126U CN201120152312U CN202078585U CN 202078585 U CN202078585 U CN 202078585U CN 2011201523126 U CN2011201523126 U CN 2011201523126U CN 201120152312 U CN201120152312 U CN 201120152312U CN 202078585 U CN202078585 U CN 202078585U
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- China
- Prior art keywords
- hollow fiber
- blood
- liver
- artificial liver
- bioreactor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- 239000012510 hollow fiber Substances 0.000 title claims abstract description 62
- 210000004185 liver Anatomy 0.000 title claims abstract description 23
- 210000004369 blood Anatomy 0.000 claims abstract description 22
- 239000008280 blood Substances 0.000 claims abstract description 22
- 210000002381 plasma Anatomy 0.000 claims abstract description 17
- 238000004113 cell culture Methods 0.000 claims abstract description 11
- 210000005229 liver cell Anatomy 0.000 claims abstract description 11
- 239000007788 liquid Substances 0.000 claims abstract description 10
- 230000002706 hydrostatic effect Effects 0.000 claims description 13
- 230000000737 periodic effect Effects 0.000 claims description 13
- 230000008676 import Effects 0.000 claims description 7
- 230000001105 regulatory effect Effects 0.000 claims description 3
- 239000012930 cell culture fluid Substances 0.000 claims description 2
- 102000004506 Blood Proteins Human genes 0.000 abstract description 3
- 108010017384 Blood Proteins Proteins 0.000 abstract description 3
- 230000000903 blocking effect Effects 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 230000002035 prolonged effect Effects 0.000 abstract 1
- 210000003494 hepatocyte Anatomy 0.000 description 7
- 230000001464 adherent effect Effects 0.000 description 6
- 230000000694 effects Effects 0.000 description 4
- 239000000835 fiber Substances 0.000 description 3
- 210000004204 blood vessel Anatomy 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 239000012531 culture fluid Substances 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 206010019663 Hepatic failure Diseases 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 208000007903 liver failure Diseases 0.000 description 1
- 231100000835 liver failure Toxicity 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
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Abstract
The utility model discloses an artificial liver bioreactor with spiral hollow fiber tubes. A plurality of hollow fiber tubes are uniformly packaged at the center in a transparent cylindrical container in parallel, inner cavities are arranged in the hollow fiber tubes, outer cavities are arranged at the outer sides of the hollow fiber tubes, patient blood plasma/blood inlets and patient blood plasma/blood outlets of the inner cavities are respectively arranged at two ends of the cylindrical container, liver cell culture liquid inlets and liver cell culture liquid outlets of the outer cavities are respectively arranged on the surface of the cylinder container in parallel, and the plurality of hollow fiber tubes are U-shaped. According to the artificial liver bioreactor, an enough area on which liver cells can be adhered on the wall for growth can be ensured, and blood in the bent parts of the U-shaped tubes can flow so as to generate secondary flow, so that the complete mixing of the blood can be facilitated, and a large quantity of plasma protein can be prevented from adhering on the tube wall so as to avoid blocking hollow fiber film holes, therefore, the detoxication and mass transfer of hollow fiber can be facilitated, the in-vitro artificial liver supporting time can be prolonged, and the curing efficiency of the artificial liver can be improved.
Description
Technical field
This utility model relates to a kind of bioreactor of artificial liver, especially relates to a kind of U-shaped hollow fiber conduit bioreactor of artificial liver.
Background technology
Bioreactor is the core apparatus of external biological artificial liver support system, is the place that exogenous hepatocyte and liver failure patient blood plasma/blood carry out mass exchange, is the key position of performance artificial liver support effect.Require to have following basic function as bioreactor: the immunity of the macromolecular substances of middle small-molecule substance transmitted in both directions and immunoglobulin intercepts, ensures hepatocyte activity and function, has a constant volume and be easy to and amplify.
At present, the bioreactor of existing application in the artificial liver system generally all is hollow-fiber bioreactor, being about to many hollow fiber conduits is packaged in the hydrostatic column, hollow fiber conduit is divided into inside and outside two chambeies with container, be called inner chamber in the hollow fiber conduit, hollow fiber conduit is called exocoel outward.Flow into patient's blood plasma/blood in the inner chamber fiber pipe, exocoel can pour into and necessarily contain hepatocellular culture fluid and be used to inoculate hepatocyte.Hepatocyte is adherent type cell, but general the tubular of hollow-fiber bioreactor all is straight tube, is unfavorable for managing the cultivation hepatocyte adherent growth of exocoel.In addition, when patient's blood plasma/blood flowed in straight tube, the flow field rule was consistent, the secondary flow phenomenon can not occur, and blood plasma/blood and exocoel can not carry out sufficient mass exchange.
Summary of the invention
The purpose of this utility model is to provide a kind of U-shaped hollow fiber conduit bioreactor of artificial liver.Inspired by interior blood vessel layout of human liver and shape, blood vessel according to bionical thought simulated liver, generally adopt at existing hollow-fiber bioreactor on the basis of straight tube, make direct attack hollow fiber pipe into the U-shaped hollow fiber conduit, can improve the support effect of external artificial liver effectively.
The technical solution adopted in the utility model is:
This utility model is a kind of U-shaped hollow fiber conduit bioreactor of artificial liver, center in transparent hydrostatic column evenly is packaged with many hollow fiber conduits side by side, in the hollow fiber conduit is inner chamber, the hollow fiber conduit outside is an exocoel, patient's blood plasma/blood inlet of inner chamber and patient's blood plasma/blood outlet is separately positioned on the two ends of hydrostatic column, and the liver cell culture liquid import regulating liver-QI cell culture fluid outlet of exocoel is set up in parallel respectively on the cylinder of hydrostatic column; It is characterized in that: described many hollow fiber conduits are many U-shaped hollow fiber conduits.
Every U-shaped pipe is made up of a plurality of periodic unit sections in the described many U-shaped hollow fiber conduits.
Described periodic unit section is that straight tube and two sections semicircle bend pipes of L are formed by two segment length, and the radius of semicircle is R.
The beneficial effect that the utlity model has is:
1, compare with direct attack hollow fiber pipe, blood samples of patients can produce secondary stream when flowing in the U-shaped hollow fiber conduit, help blood and fully mix, abundant and tube wall effect, the therapeutic efficiency of raising artificial liver.
2, compare with direct attack hollow fiber pipe, blood samples of patients flows in the U-shaped hollow fiber conduit and can produce secondary stream, can prevent that macromole such as plasma protein from sticking to the tube wall place and stopping up the fiber fenestra, alleviating film pollutes, the detoxifcation, the mass transfer that help hollow-fibre membrane, the outer artificial rami hepatici of extension body is held action time.
3, compare with direct attack hollow fiber pipe, the external surface area of U-shaped hollow fiber conduit is bigger, helps the hepatocellular adherent growth of adherent type.
Description of drawings
Fig. 1 is a U-shaped hollow fiber conduit bioreactor of artificial liver sketch map.
Fig. 2 is U-shaped hollow fiber conduit periodic unit section parameter calibration figure.
Among the figure: 1, patient's blood plasma/blood inlet; 2, patient's blood plasma/blood outlet; 3, liver cell culture liquid import; 4, liver cell culture liquid outlet; 5, exocoel; 6, hydrostatic column; 7, U-shaped hollow fiber conduit.
The specific embodiment
Below in conjunction with drawings and Examples this utility model is further described.
As shown in Figure 1, center in transparent hydrostatic column 6 evenly is packaged with many hollow fiber conduits side by side, in the hollow fiber conduit is inner chamber, the hollow fiber conduit outside is an exocoel, patient's blood plasma/blood inlet 1 of inner chamber and patient's blood plasma/blood outlet 2 is separately positioned on the two ends of hydrostatic column 6, and the liver cell culture liquid import 3 regulating liver-QI cell culture fluids outlet 4 of exocoel is set up in parallel respectively on the cylinder of hydrostatic column 6; It is characterized in that: described many hollow fiber conduits are many U-shaped hollow fiber conduits.
As shown in Figure 2, every U-shaped pipe is made up of a plurality of periodic unit sections in the described many U-shaped hollow fiber conduits.
Described periodic unit section is that straight tube and two sections semicircle bend pipes of L are formed by two segment length, and the radius of semicircle is R.
As shown in Figure 1, the profile of present embodiment bioreactor of artificial liver is cylindrical, transparent plastic shell, i.e. and hydrostatic column 6 among the figure, its size dimension can amplify or dwindle according to the purposes needs, formulates needed size, gets long 15 as present embodiment
Cm, cross section external diameter 5
Cm, cross sectional inner diameter 4
CmAs shown in Figure 1, patient's blood plasma/blood enters the inner chamber in the U-shaped hollow fiber conduit 7 by import 1; Patient's blood plasma/blood flow flows out through exporting 2 behind U-shaped hollow fiber conduit 7 inner chambers; 3 is the import of liver cell culture liquid, promptly injects liver cell culture liquid to exocoel 5 by this import, is used for the hepatocyte inoculation; 4 for the outlet of liver cell culture liquid, can take away the part toxin, the sample tap when being treatment simultaneously.
As shown in Figure 2, U-shaped hollow fiber conduit periodic unit section parameter calibration figure.The periodic unit section is made up of two sections straight tubes and two sections semicircle bend pipes, and straight tube alternately is connected with the semicircle bend pipe, as shown in Figure 2, and wherein
aBe the hollow fiber conduit radius,
LBe length of straight pipe,
RBe the radius of bend pipe semicircle, can determine the basic size of U-shaped pipe by these three parameters.Every U-shaped hollow fiber conduit is made up of a plurality of periodic unit sections, and the total length of U-shaped hollow pipe is determined according to hydrostatic column length.The concrete parameter size of U-shaped pipe hollow fiber conduit periodic unit section can be amplified or dwindles according to the purposes needs, gets as present embodiment
aBe 1mm,
LBe 10mm,
RBe 5mm.What need supplementary notes is that the every U-shaped hollow fiber conduit of this implementation column is by a plurality of periodic unit sections, but also can be made up of a plurality of non-periodic cells sections, can be made up of the different elementary section of a plurality of R values as the U-shaped hollow fiber conduit.
U-shaped hollow fiber conduit exterior surface area is than long-pending big many of direct attack hollow fiber tube outer surface, can provide competent area allow adherent type hepatocyte adherent growth; The important factor of another one is that blood plasma/blood is when flowing in the U-shaped hollow fiber conduit, can produce secondary stream in that canal curvature is local, blood fully be mixed and tube wall fully acts on, prevent that macromole such as plasma protein from sticking to tube wall, comprehensively improves the therapeutic efficiency of artificial liver.
The above-mentioned specific embodiment is used for this utility model of explaining; rather than this utility model limited; in the protection domain of spirit of the present utility model and claim, any modification and change to this utility model is made all fall into protection domain of the present utility model.
Claims (3)
1. U-shaped hollow fiber conduit bioreactor of artificial liver, center in transparent hydrostatic column (6) evenly is packaged with many hollow fiber conduits side by side, in the hollow fiber conduit is inner chamber, the hollow fiber conduit outside is an exocoel, patient's blood plasma/blood inlet (1) of inner chamber and patient's blood plasma/blood outlet (2) is separately positioned on the two ends of hydrostatic column (6), and liver cell culture liquid import (3) the regulating liver-QI cell culture fluid outlet (4) of exocoel is set up in parallel respectively on the cylinder of hydrostatic column (6); It is characterized in that: described many hollow fiber conduits are many U-shaped hollow fiber conduits.
2. a kind of U-shaped hollow fiber conduit bioreactor of artificial liver according to claim 1 is characterized in that: every U-shaped pipe is made up of a plurality of periodic unit sections in the described many U-shaped hollow fiber conduits.
3. a kind of U-shaped hollow fiber conduit bioreactor of artificial liver according to claim 2 is characterized in that: described periodic unit section is that straight tube and two sections semicircle bend pipes of L are formed by two segment length, and the radius of semicircle is R.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011201523126U CN202078585U (en) | 2011-05-13 | 2011-05-13 | Artificial liver bioreactor with U-shaped hollow fiber tubes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011201523126U CN202078585U (en) | 2011-05-13 | 2011-05-13 | Artificial liver bioreactor with U-shaped hollow fiber tubes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202078585U true CN202078585U (en) | 2011-12-21 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011201523126U Expired - Fee Related CN202078585U (en) | 2011-05-13 | 2011-05-13 | Artificial liver bioreactor with U-shaped hollow fiber tubes |
Country Status (1)
| Country | Link |
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| CN (1) | CN202078585U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102225222A (en) * | 2011-05-13 | 2011-10-26 | 浙江大学 | U-shaped hollow fiber pipe artificial liver bioreactor |
| CN103100119A (en) * | 2013-01-24 | 2013-05-15 | 中山大学 | Artificial liver bioreactor |
| US11306281B2 (en) * | 2016-05-19 | 2022-04-19 | Koji Saito | Culture device, culture method and cultured organ produced by the culture method |
-
2011
- 2011-05-13 CN CN2011201523126U patent/CN202078585U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102225222A (en) * | 2011-05-13 | 2011-10-26 | 浙江大学 | U-shaped hollow fiber pipe artificial liver bioreactor |
| CN103100119A (en) * | 2013-01-24 | 2013-05-15 | 中山大学 | Artificial liver bioreactor |
| US11306281B2 (en) * | 2016-05-19 | 2022-04-19 | Koji Saito | Culture device, culture method and cultured organ produced by the culture method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20111221 Termination date: 20130513 |