CN201707334U - Device for implementing immune nano particle chromatographic determination method - Google Patents
Device for implementing immune nano particle chromatographic determination method Download PDFInfo
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- CN201707334U CN201707334U CN2010201513577U CN201020151357U CN201707334U CN 201707334 U CN201707334 U CN 201707334U CN 2010201513577 U CN2010201513577 U CN 2010201513577U CN 201020151357 U CN201020151357 U CN 201020151357U CN 201707334 U CN201707334 U CN 201707334U
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Abstract
The utility model provides a device for implementing an immune nano particle chromatographic determination method, which comprises at least one chromatographic column. The chromatographic column is provided with an upper sample adding zone, a middle active zone and a lower drainage zone, wherein the active zone is filled with an immunosorbent used for capturing and fixing a substance to be determined. The device has simple structure and convenient and fast operation; the detection sensitivity is high by applying the device to implement immune nano particle chromatographic determination, and moreover, multiple items can be determined at a time.
Description
Technical field
The utility model is the device of using about a kind of immunoassay, specifically is meant a kind of device that is used to implement immune nano particle method of measurement in chromatography, and it comprises the chromatographic column through particular design.
Background technology
The immune analysis method that is used for ultramicron now has radiommunoassay, enzyme-linked immuno assay, fluoroimmunoassay, time resolved fluoro-immunoassay, chemiluminescence immune assay etc., these analytical approachs all need antibody or antigen are passed through the corresponding material (for example iodine-125, horseradish peroxidase, fluorescein, rare earth element, chemiluminescent substance etc.) that produces distinctive signal on the chemical method mark, by corresponding instrument or the described distinctive signal of Equipment Inspection.This operation is cumbersome.And, described label is in the immunoassay process, it is immunoreagent and participate in immune response directly, carry the material that produces distinctive signal again, it is the key substance in the ultramicron immunoassays, in a single day its quality is affected, bring very disadvantageous effect will for the quality of kit, even lead to the failure, and in the chemical method labeling process, usually will be through peroxidating, the effect of chemical reactions such as reduction, what have also will be subjected to radiation damage (labelled with radioisotope), the immunological characteristic of these processes meeting antagonists or antigen causes infringement to a certain degree, and this will inevitably reduce the quality of detection kit.And above-mentioned immune analysis method is once tested and all can only be detected a project or index.
Spot immune gold diafiltration determination method (the gold-marking immunity percolation that development in recent years is got up, DotImmunogold Filtration Assay, DIGFA) though simplified method of operating, shortened the reaction time, operate fast and convenient, but because the little limitation of " spot " amount, its detection sensitivity does not reach desirable level yet.The relevant detection device wherein is in advance test item to be fixed on test paper or the chip for detecting test paper or detection chip, can not change test item or index, uses underaction.
The utility model content
Designer of the present utility model is at the existing deficiency of existing immune analysis method, research provides a kind of super quick immune analysis method that nanometer technology and immunochromatography technique are combined, can be described as immune nano particle measurement in chromatography (Immunonanoparticles chromatographic assay, INCA) method, fundamental purpose of the present utility model is to provide a kind of device that is used to implement this immune nano particle method of measurement in chromatography, improves detection sensitivity.
For reaching above-mentioned purpose, the utility model provides a kind of device that is used to implement immune nano particle method of measurement in chromatography, this device comprises at least one chromatographic column, described chromatographic column is provided with epimere sample application zone, active region, stage casing and hypomere drainage region, is filled with in the active region, described stage casing to be used to catch the fixedly immunosorbent of test substance.
In the utility model, the design feature of chromatographic column design is to have huge immunity to concentrate (immunoconcentration) effect.According to specific embodiments of the present utility model, in the utility model, described chromatographic column epimere sample application zone connects by the undergauge structure to the active region, stage casing, active region, described stage casing connects by the undergauge structure to the hypomere drainage region, described chromatographic column epimere sample application zone internal diameter 6～10mm, preferred 10～the 14mm of high 8～14mm, active region, stage casing internal diameter 2～4mm, high 6～10mm, hypomere drainage region internal diameter 1～2mm, high 2～4mm, the epimere sample application zone excessively arrives the high 3～5mm of undergauge structure division of active region, stage casing, and the high 2～4mm of undergauge structure division of hypomere drainage region is excessively arrived in the active region, stage casing.In this embodiment, epimere mainly is to be used for application of sample; The active region that is filled with specific immunosorbent that the stage casing is thin and long, can be effectively with corresponding antibody in the testing sample or antigen capture, concentrate, gather, and measure; Hypomere mainly is to be used to discharge residue (waste liquid), the tiny effect that the control flow velocity is arranged in aperture.
According to specific embodiments of the present utility model, in the utility model, the two ends up and down of active region, described chromatographic column stage casing also can be embedded with the port internal diameter of the two ends up and down porous disk of the same size with active region, chromatographic column stage casing, in order to the fixing immunosorbent of active region, be appreciated that, the thickness of porous disk is got over Bao Yuehao, general thick 1mm or thinner under the prerequisite that guarantees porous disk support strength.In addition, all can be provided with elastic colloid at the chromatographic column upper and lower end and seal cap, be convenient to seal and vacuumize, be beneficial to preservation.
According to specific embodiments of the present utility model, in the utility model, the active region structure of described chromatographic column can have various ways, as long as have concentrated, compacting effect also has enough capacity to get final product, concentrate, the effect gathered is that extremely rare measured object is concentrated in together, enough capacity are that the amount that ensures the measured object of being caught can be detected, for example the active region can be made the porous cylindrical body of no blind hole, square cylinder, or be filled with disk, side's sheet, ball, ellipsoid or do not have the rule or the erose porous medium etc. of blind hole, it also can be porous (plastics) sponge of filling no blind hole, microballoon, the glass sand of sintering, cellulose (crystallite, film etc.), glucosan, agarose ... or the like, allly can be used for the active region that material that physics or chemical method prepare immunosorbent all can be used for processing chromatographic column.
According to specific embodiments of the present utility model, in the utility model, described chromatographic column bottom is provided with the structure that this chromatographic column is connected in series with another chromatographic column stack.The described structure that chromatographic column and the stack of another chromatographic column are connected in series can be buckle structure or spiro connection structure.For example, in a preferred embodiment of the present utility model, be provided with external thread in described chromatographic column epimere cylinder appearance side, the lower ends downward of this chromatographic column epimere cylinder is extended a tube structure, and this extended tube structure is provided with internal thread, the internal thread of this extended tube structure can be realized connecting of two chromatographic columns with the external thread bolt connection of another chromatographic column epimere cylinder appearance side, and the bottom of the chromatographic column above the series connection back is higher than the upper end of the active region of following chromatographic column.
According to specific embodiments of the present utility model, in the utility model, the active region in described chromatographic column stage casing is divided into a plurality of districts band, is filled with the immunosorbent that is used to catch fixing different test substances respectively, and the separation layer of non-activity is arranged between each district's band.
When using device of the present utility model and implementing immune nano particle method of measurement in chromatography, can carry out in accordance with the following methods: testing sample is added described chromatographic column from sample application zone, and the described active region of flowing through; The solution that will be suspended with nano particle then adds chromatographic column from described sample application zone, and the described active region of flowing through, and wherein said nano particle is coated with the material that can combine with described test substance.Described nano particle can be luminescent nanoparticle, also can be chromonic nanoparticles.After the solution that will be suspended with nano particle adds the chromatographic column and the described active region of flowing through from described sample application zone, can carry out qualitative or quantitative test to test substance in the sample according to change color or change in fluorescence in the chromatographic column active region.Also can increase the testing sample amount of the described active region of flowing through as required, test substance in the sample be carried out qualitative or quantitative test according to change color or change in fluorescence in the chromatographic column active region.
Utilize the device that comprises chromatographic column of the present utility model to carry out immune nano particle method of measurement in chromatography, measuring principle mainly can be used various immunizations such as sandwich method, competition law etc.
For example, use the hepatitis B virus pre S 1 antigen (HBVPre S1Ag) in the sandwich method for determining principle working sample, fill the immunosorbent that is coated with HBV Pre S1Ab (S1 antibody) of q.s in the active region of chromatographic column, after adding testing sample, when testing sample is flowed through the active region, if contain HBV Pre S1Ag in the testing sample, this HBV Pre S1Ag can be with its antibodies in the active region and is fixed; Add the Anti-HBs that carries luminescent nanoparticle (as fluorescent nano particles) then, can combine with the S1 antigen on being fixed on antibody when it flows through the active region, form the immune complex that has fluorescence; There is not the luminescent nanoparticle of combination to separate, by measuring fluorescence intensity is whether to have S1 antigen in the decidable institute test sample product through the washing lotion washing.Obviously, antigenic content and fluorescence intensity are proportionate in the sample, in view of the above can with measured result with compare with the calibration object of concentration known, but the HBV Pre S1Ag content in the quantitative measurement sample; Also calibration object can be changed into feminine gender, positive reference substance, can do qualitative determination.
For another example, adopt indirect method promptly to adopt anti-cytomegalovirus IgG antibody in the two anti-sandwich method for determining samples (Anti-CMV[IgG]), fill the immunosorbent that is coated with CMV antigen of q.s in the active region, after testing sample is added chromatographic column, sample flow is crossed the active region that is coated with antigen, if have Anti-CMV (IgG) in the sample, it will combine with its antigen and be fixed; Add the Anti-IgG (people) carry luminescent nanoparticle (as fluorescent nano particles) then, combine, form the immune complex that has fluorescence with IgG on being fixed on antigen; The fluorescent particles that does not have combination is removed through washing; Measuring fluorescence intensity is whether to have corresponding antibodies in the decidable blood serum sample.Obviously, antigenic content and fluorescence intensity are proportionate in the sample, in view of the above can with measured result with compare with the calibration object of concentration known, but the content of the antibody in the quantitative measurement sample; Also calibration object can be changed into feminine gender, positive reference substance, can do qualitative determination.
The scheme of above-mentioned application sandwich method for determining principle is when practical measurement, if test substance content is small in the testing sample, only need application of sample several times, promptly repeat to add the step of sample, luminescent nanoparticle and washing lotion, can directly measure fluorescence signal, save traditional incubation and separable programming.
And for example, be that example illustrates the concrete operations of using the competition law measuring principle with the free thyroxine in the working sample (FT4).It wherein is the immunosorbent that is coated with Anti-T4 (mouse monoclonal antibody) of filling q.s in the active region, after testing sample is added chromatographic column, sample flow is crossed the active region that is coated with antibody, FT4 in the sample will be fixed with its antibodies, the Anti-IgG that carries luminescent nanoparticle (as fluorescent nano particles) (mouse) that adds q.s then, with remaining antibodies on the immunosorbent, form the immune complex that has fluorescence; The fluorescent particles that does not have combination is removed through washing; Measuring fluorescence intensity is the concentration of FT4 in the decidable sample.Obviously, F T4 content and fluorescence intensity are negative correlation in the sample, and the calibration object with concentration known compares in view of the above, can quantitatively predict the content of FT4 in the sample.
Described luminescent nanoparticle can substitute with chromonic nanoparticles.With change color result of determination in the eye-observation chromatographic column active region, can carry out qualitative or quantitative test to test substance in the sample.Chromonic nanoparticles can or have a liking for selecting as required different colors.For example, according to human eye to the sensitive part of photochromic coloured silk near 490nm and 590nm and the aberration resolution characteristic, when doing solid phase carrier, select for use nano particles (as nano ceramics, nano-nickel oxide, nm of gold etc.) such as (grass) green, cyan, orange-yellow, redness more responsive usually with transparent or semitransparent thing; When doing solid phase carrier, with black nano particle (as Nano Silver, copper, iron etc.) preferable (aberration resolution) with white thing.
Use device of the present utility model and implement immune nano particle measurement in chromatography, wherein in conjunction with having used nanometer technology and immunochromatography technique, detection sensitivity height.
Utilize device of the present utility model to carry out immune nano particle measurement in chromatography, can once test the detection many index.The active region that can be the stage casing of chromatographic column described in the use device is divided into a plurality of districts band, is filled with the immunosorbent that is used to catch fixing different test substances respectively, and the separation layer of non-activity is arranged between each district's band.Utilize the device that comprises chromatographic column of the present utility model once to test to detect 5 antibody indexs of Torch is example, in this scheme, described chromatographic column active region is divided into 5 district's bands, each district's band detects 1 project (1 index), be followed successively by from top to bottom and measure Anti-CMV (IgG), Anti-HSV-I (IgG), Anti-HSV-II (IgG), Anti-RV (IgG) and Anti-TOXO (IgG), the filling material of first district band is coated with CMV, the filling material of second district band is coated with HSV-I, the filling material of the 3rd district band is coated with HSV-II, the filling material of the 4th district band is coated with RV, and the filling material of the 5th district band is coated with TOXO; The separation layer that non-activity is arranged between each district's band is so that observations.The antigen of each district's band combines with corresponding antibody respectively during mensuration.(adopting two anti-sandwich methods is example, and the practical measurement method can be carried out in two steps: add sample and flow through chromatographic column to adopt indirect method; Add two anti-currents that are stained with nano particle after the washing and cross chromatographic column, the color result of determination of each district's band of chromatographic column is observed in the washing back.Particularly, after testing sample was added chromatographic column, sample flow through 5 district's bands that are coated with antigen successively, if having corresponding antibody in the sample, will combine with antigen separately and be fixed; Add the Anti-IgG (people) carry chromonic nanoparticles (as nano oxidized nickel particles) then, combine, form (grass) green immunization compound with IgG on being fixed on antigen; The colored particle that does not have combination separates through washing; Color with each district's band of eye-observation is whether to have corresponding antibodies in the decidable institute test sample product.Obviously, certain antibody in the sample is many more, anti-just many more in conjunction with last two, thereby it is just many more in conjunction with (grass) green particles that gets on, be that the colour band color is dark more, long more, that is to say that the degree of depth and the length of the colour band color of antibody content and formation in the sample are proportionate, calibration object with concentration known compares in view of the above, and also available in principle chromatographic scan quantitatively predicts the content of antibody in the sample.
According to specific embodiments of the present utility model, also can be that described chromatographic column bottom is provided with the structure that this chromatographic column and the stack of another chromatographic column are connected in series, the chromatographic column by a plurality of serial connections carries out a plurality of projects of measuring one time.
According to specific embodiments of the present utility model, the device that is used to implement immune nano particle method of measurement in chromatography of the present utility model is a kind of kit, and this kit comprises: a plurality of described chromatographic columns; Negative control, positive control reagent and/or calibration reagent; Nm of gold; And corresponding washing lotion.
Device of the present utility model is designed to the chromatographic column of each independent stackable again (series connection) in the practical application, can be arbitrarily made with according to user's needs multinomially once to measure, and measures convenient.
In sum, concluding main beneficial effect advantage of the present utility model comprises:
Chromatographic column of the present utility model, active region band very thin (narrow) and growing has the function that concentrates and gather measured matter, the detection sensitivity height;
Chromatographic column of the present utility model can be preserved the original residing environment of used key reagents (antibody, antigen etc.), helps preserving, and has increased stability;
The utility model is simple in structure, and the preparation difficulty is low, and the loss that this had both been avoided expensive raw material has reduced production cost, has also reduced work and time;
Chromatographic column of the present utility model can be designed to series connected style, can a measuring many index, than superior the fixing dead immune detection chip of test item in advance, the one, highly sensitive, the 2nd, applying flexible can carry out once multinomial mensuration by random as required stack combinations chromatographic column;
Chromatographic column of the present utility model is by immune inspissation, make the measured object that is present in the sample such as certain antigen the zero distance touch opportunity be arranged with corresponding antibody on the solid phase carrier, shortened the time of antigen-antibody binding reaction greatly, detected fast, whole detection can be finished in a few minutes.
Description of drawings
Fig. 1 is the structural representation of chromatographic column in the device of the present utility model.
Fig. 2 is the structural representation of two chromatographic columns series connection of the present utility model.
Embodiment
Further describe the technical solution of the utility model below by specific embodiment, but therefore the utility model is not subjected to any restriction.
Embodiment 1
See also shown in Figure 1ly, present embodiment provides a kind of chromatographic column, and this chromatographic column is provided with epimere sample application zone 11, active region, stage casing 12 and hypomere drainage region 13, and these three sections are cylindric.Described epimere sample application zone 11 arrives active region, stage casing 12 and connects by the first undergauge structure 14, and 15 connections of the second undergauge structure are passed through to hypomere drainage region 13 in active region, described stage casing 12.Epimere sample application zone 11 internal diameter 8mm, high 8mm, active region, stage casing 12 internal diameter 2mm, high 10mm, be filled with in the active region and be used to catch the fixedly immunosorbent of test substance, the epimere sample application zone excessively arrives first diameter shrinkage part, the 14 high 4mm of active region, stage casing, hypomere drainage region 13 internal diameter 1mm, high 6mm, second diameter shrinkage part, 15 high about 3mm of hypomere drainage region are excessively arrived in the active region, stage casing.The two ends up and down of active region, stage casing 12 are embedded with and corresponding position chromatographic column internal diameter porous disk 16,17 of the same size (diameter of the porous disk 16 of the comparable upper port of porous disk 17 diameters of active region, stage casing lower port is smaller), in order to the fixing immunosorbent of active region; The chromatographic column upper and lower side all can be provided with elastic colloid and seal cap 18,19, is convenient to seal and vacuumize, and is beneficial to preservation (should take when using chromatographic column and seal cap).In addition, active region, stage casing cylinder and hypomere drainage region cylinder also can be designed to respectively with its on the excessive structure that is spirally connected mutually of the undergauge structure of section, cleaning for convenience detach.The lower ends downward of the chromatographic column epimere sample application zone cylinder 11 that shows among Fig. 1 is extended the tube structure 20 that is looped around outside the described first undergauge structure 14, and this tube structure 20 is provided with internal thread, and the internal thread of the described tube structure 20 of this chromatographic column can be realized connecting of two chromatographic columns with the external thread bolt connection of another chromatographic column epimere cylinder appearance side.
Embodiment 2
See also shown in Figure 2, the chromatographic column that comprises two series connection in the device of present embodiment, each chromatographic column is provided with epimere sample application zone 11, active region, stage casing 12 (be filled with in the active region, described stage casing and be used to catch the fixedly immunosorbent of test substance) and hypomere drainage region 13, described epimere sample application zone 11 arrives active region, stage casing 12 and connects by the first undergauge structure 14, and 15 connections of the second undergauge structure are passed through to hypomere drainage region 13 in active region, described stage casing 12; The epimere internal diameter is about 10mm, high about 12mm; Stage casing internal diameter 4mm, high 6mm, the high about 4mm of first diameter shrinkage part, the high about 3mm of second diameter shrinkage part; Hypomere internal diameter 1mm, high 2mm; The two ends up and down in stage casing are embedded with and corresponding position chromatographic column internal diameter porous disk 16,17 of the same size, and in order to the fixing immunosorbent of active region, the chromatographic column upper and lower side all can be provided with and seal cap 18,19; The lower ends downward of described chromatographic column epimere cylinder 11 is extended the tube structure 20 that is looped around outside the described first undergauge structure 14, and this tube structure 20 is provided with internal thread, top chromatographic column is realized connecting of two chromatographic columns by the internal thread of described tube structure 20 with the external thread bolt connection of following another chromatographic column epimere cylinder appearance side, and the bottom of the chromatographic column above the series connection back is higher than the upper end of the active region of following chromatographic column.
HBsAg index in application example 1, the working sample
1. reagent is formed
The series calibration object, each 1 bottle;
Measure the HBsAg chromatographic column, 50 or 100, its structure such as embodiment 1;
Fluorescent nano particles (being coated with Anti-HBs), 1 bottle;
Concentrate washing lotion, 1 bottle.
2. mensuration program
(1). take off chromatographic column capping up and down, establish calibration object and sample chromatographic column;
(2). add calibration object, sample 200 μ l separately in the chromatographic column, treat that sample liquid flows through chromatographic column active region band after, the washing lotion 500 μ l that add after the dilution wash pipe;
(3). each pipe adds fluorescent nano particles 100 μ l, treat that sample liquid flows through chromatographic column active region band after, the same hole flushing;
(4). measure the fluorescence intensity of each post with special-purpose fluor tester.
3. the result judges
(1). make the dose-response curve of calibration object by log (dose)～log (B-Bo), require correlation coefficient r＞0.9900, experiment is reliable;
(2). obtain the HBsAg concentration of sample from dose-response curve;
(3). normal value reference: normal human serum＜0.05IU/ml.
HBsAg and two indexs of HBeAg in application example 2, the measuring sample
1. kit is formed
1.1. the positive and negative contrast, each 1 bottle;
1.2. measure HBsAg and HBeAg chromatographic column, its structure is with embodiment 2; Each 50 or 100 cover (per two chromatographic columns that are cascaded are a cover);
1.3. nm of gold (being coated with Anti-HBs and Anti-HBe respectively), each 1 bottle;
1.4. concentrate washing lotion, 1 bottle.
2. kit preparation
2.1. the preparation of chromatographic column
2.1.1. the elementary structure parameter of chromatographic column sees also embodiment 2.
2.1.2. preparation immunosorbent (immunoadsorbents)
(￠＜1mm) is a carrier, and with the physisorphtion preparation, microballoon be covered respectively Anti-HBs and Anti-HBe remove coating buffer, dry standby after the sealing with the Polyvinylchloride of clear, colorless or nylon micro-sphere or polystyrene.
2.1.3. assembling chromatographic column
Put a porous sequin into post stage casing bottom, immunosorbent is filled it up with in the stage casing, and down is gone into porous sequin (fixation), and two ends are built and sealed cap (nontoxic rubber), take out appropriate vacuum from last cap after, post label at epimere.Preserve down in 2～8 ℃.
2.2. antibody nm of gold
2.2.1.Anti-HBs nm of gold (also commercially available acquisition)
With 10ml concentration is 0.1mg/ml chlorauric acid solution heated and boiled, adding concentration is 10mg/ml trisodium citrate 0.15ml, transfer pH to 6.0 with 0.1mol/L HCl, add Anti-HBs 0.1mg, stir 10min, the centrifugal 20min of 3000r/min, get supernatant, the centrifugal 20min of 12000r/min is suspended from precipitation among the PBS that 100ml (debugging) contains 1%BSA, 0.2mg/ml NaN3, every bottle of packing 10ml posts the label postposition and preserves down for 2～8 ℃.
2.2.2.Anti-HBe nm of gold
Logical 2.2.1 item preparation.
2.3. reference substance
Positive control contains an amount of HBsAg and HBeAg, and negative control is that normal human serum and calf serum mix.Respectively press the packing of 1ml/ bottle.Preserve down in 2～8 ℃ after posting label.
2.4. concentrate washing lotion
Ditto.
3. kit uses
3.1. mensuration program
3.1.1. take off chromatographic column up and down seal cap, 2 layers (referring to Fig. 2) formed in stack, establishes contrast and sample chromatographic column;
3.1.2. add contrast, sample 200 μ l separately in the chromatographic column, treat that sample liquid flows through lower floor's chromatographic column district band after, washing lotion 500～1000 μ l that add after the dilution wash pipe;
3.1.3. each pipe adds 4 of nm of gold (about 200 μ l), treat that sample liquid flows through the 1st floor chromatographic column district band after, the same hole flushing;
3.2. the result judges
3.2.1. the positive control pipe shows 2 red zones, and the negative control pipe is colourless substantially, experiment is reliable;
3.2.2. it is positive to show the sample hose of red zone, judges it is HBsAg and HBeAg by district's band;
3.2.3. can do roughly to quantitatively judge according to the depth, the length of colour band, color depth and long positive strong.
Claims (10)
1. device that is used to implement immune nano particle method of measurement in chromatography, it is characterized in that: this device comprises at least one chromatographic column, described chromatographic column is provided with epimere sample application zone, active region, stage casing and hypomere drainage region, is filled with in the active region, described stage casing to be used to catch the fixedly immunosorbent of test substance.
2. the device that is used to implement immune nano particle method of measurement in chromatography according to claim 1, it is characterized in that: described chromatographic column epimere sample application zone connects by the undergauge structure to the active region, stage casing, active region, described stage casing connects by the undergauge structure to the hypomere drainage region, described chromatographic column epimere sample application zone internal diameter 6～10mm, high 8～14mm, active region, stage casing internal diameter 2～4mm, high 6～10mm, hypomere drainage region internal diameter 1～2mm, high 2～4mm, the epimere sample application zone excessively arrives the high 3～5mm of undergauge structure division of active region, stage casing, and the high 2～4mm of undergauge structure division of hypomere drainage region is excessively arrived in the active region, stage casing.
3. the device that is used to implement immune nano particle method of measurement in chromatography according to claim 1 is characterized in that: the two ends up and down of active region, described chromatographic column stage casing are embedded with the port internal diameter of the two ends up and down porous disk of the same size with active region, chromatographic column stage casing.
4. the device that is used to implement immune nano particle method of measurement in chromatography according to claim 1 is characterized in that: described chromatographic column upper and lower end is provided with elastic colloid and seals cap.
5. the device that is used to implement immune nano particle method of measurement in chromatography according to claim 1, it is characterized in that: the active region, stage casing of described chromatographic column is porous cylindrical body, the square cylinder of no blind hole, or is filled with disk, square sheet, ball, ellipsoid or does not have the porous medium of blind hole.
6. the device that is used to implement immune nano particle method of measurement in chromatography according to claim 1 is characterized in that: described chromatographic column bottom is provided with the structure that this chromatographic column is connected in series with another chromatographic column stack.
7. the device that is used to implement immune nano particle method of measurement in chromatography according to claim 6 is characterized in that: described is buckle structure or spiro connection structure with chromatographic column with the structure that another chromatographic column stack is connected in series.
8. the device that is used to implement immune nano particle method of measurement in chromatography according to claim 7, it is characterized in that: be provided with external thread in described chromatographic column epimere cylinder appearance side, the lower ends downward of this chromatographic column epimere cylinder is extended a tube structure, and this extended tube structure is provided with internal thread, the internal thread of this extended tube structure can be realized connecting of two chromatographic columns with the external thread bolt connection of another chromatographic column epimere cylinder appearance side, and the bottom of the chromatographic column above the series connection back is higher than the upper end of the active region of following chromatographic column.
9. the device that is used to implement immune nano particle method of measurement in chromatography according to claim 1, it is characterized in that: the active region in described chromatographic column stage casing is divided into a plurality of districts band, be filled with the immunosorbent that is used to catch fixing different test substances respectively, be provided with the separation layer of non-activity between each district's band.
10. device according to claim 1, this device is kit.
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| CN2010201513577U CN201707334U (en) | 2010-04-02 | 2010-04-02 | Device for implementing immune nano particle chromatographic determination method |
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| CN2010201513577U CN201707334U (en) | 2010-04-02 | 2010-04-02 | Device for implementing immune nano particle chromatographic determination method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102213717A (en) * | 2010-04-02 | 2011-10-12 | 王立莉 | Immune nano-particle chromatographic measuring method and device for implementing method |
| CN110376373A (en) * | 2019-08-23 | 2019-10-25 | 王爱芳 | A kind of colloidal gold accelerates reaction unit and detection method |
-
2010
- 2010-04-02 CN CN2010201513577U patent/CN201707334U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102213717A (en) * | 2010-04-02 | 2011-10-12 | 王立莉 | Immune nano-particle chromatographic measuring method and device for implementing method |
| CN110376373A (en) * | 2019-08-23 | 2019-10-25 | 王爱芳 | A kind of colloidal gold accelerates reaction unit and detection method |
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