CN207528271U - A kind of water quality monitoring biosensor - Google Patents
A kind of water quality monitoring biosensor Download PDFInfo
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- CN207528271U CN207528271U CN201721683465.7U CN201721683465U CN207528271U CN 207528271 U CN207528271 U CN 207528271U CN 201721683465 U CN201721683465 U CN 201721683465U CN 207528271 U CN207528271 U CN 207528271U
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Abstract
The utility model discloses a kind of water quality monitoring biosensor, including bod probe, acid-base value probe, temp probe, dissolved oxygen probe, signal acquisition process device, wireless communication module, constent temperature heater, calibration liquid case, liquid case to be measured, cleaning fluid tank, buffering liquid case, reactive tank, the first peristaltic pump and the second peristaltic pump.The bod probe is integrated with biosensor, and biosensor is immobilized microorganism bead sensing module.The utility model is popped one's head in by acid-base value, temp probe, dissolved oxygen probe, bod probe realize monitoring to acid-base value in city river, temperature, dissolved oxygen, bod and organic matter oxygen demand, available for the water quality monitoring using ammonia nitrogen as major pollutants city river.Zigbee module, Wi Fi modules, bluetooth module are combined by wireless communication module with CDMA module, GPRS module in the utility model meets the local of data and remote transmission requirement.
Description
Technical field
The utility model is related to water quality monitoring technical field more particularly to a kind of water quality monitoring biosensors.
Background technology
City river water quality environment and people life are closely bound up, construction of the China to water body environment monitoring system, mainly
Some main rivers and lake are concentrated on, establishes relevant water quality monitoring network station, what these water quality monitoring stations generally used
It is that artificial chemical detection is carried out to water body, this method is not only cumbersome, and water quality cannot be carried out effective in real time quick
Analysis.City river is not directed to also especially with the fast slowdown monitoring of water quality of the more prominent city river of ammonia and nitrogen pollution at present
The method and apparatus of property.City river has its significant contamination characteristics, for different zones city river, develops corresponding water quality
Monitoring device has certain significance and importance.
Utility model content
The purpose of this utility model is that by a kind of water quality monitoring biosensor, to solve background section above
The problem of mentioning.
For this purpose, the utility model uses following technical scheme：
A kind of water quality monitoring biosensor, including：Bod (BOD) probe, acid-base value (pH) probe, temperature
(T) probe, dissolved oxygen (DO) probe, signal acquisition process device, wireless communication module, constent temperature heater, calibration liquid case, prepare liquid
Case, cleaning fluid tank, buffering liquid case, reactive tank, the first peristaltic pump, the second peristaltic pump, aerator and exhausted bath box；The biochemistry
Oxygen demand probe is arranged in reactive tank；The acid-base value probe, temp probe, dissolved oxygen probe are arranged on the liquid case to be measured
It is interior；The bod probe, acid-base value probe, temp probe, dissolved oxygen probe and signal acquisition process device connect；It is described
Wireless communication module connection signal Acquisition Processor, and connect with application terminal wireless telecommunications；The bod pops one's head in, is anti-
Slot is answered to be configured with constent temperature heater；The solution injection reaction that first peristaltic pump will demarcate liquid case, liquid case to be measured, cleaning fluid tank
Slot；The solution for buffering liquid case is injected reactive tank by second peristaltic pump；The aerator and exhausted bath box coupled reaction slot；Institute
It states bod probe and is integrated with biosensor, the biosensor perceives mould for immobilized microorganism bead
Block.
Particularly, the microorganism selected in the immobilized microorganism bead sensing module is the gemma bar of orientation domestication
Bacterium.
Particularly, Zigbee module, Wi-Fi module, bluetooth module, CDMA module are integrated in the wireless communication module
And GPRS module.
Particularly, the wireless communication module passes through USB2.0 interface connection signal Acquisition Processors.
Particularly, the wireless communication module uses macro electricity DTU-H7710 data transmission units.
Particularly, the signal acquisition process device includes the microcontroller using ARM9 embeded processors.
The utility model proposes water quality monitoring biosensor mesophytization oxygen demand probe be integrated with biosensor,
Biosensor is immobilized microorganism bead sensing module, and microorganism is fixed on by immobilized microorganism bead sensing module
On choosing card carrier, make its highly dense and keep bioactivity, so as to fulfill the monitoring of city river mesophytization oxygen demand；This reality
With it is novel popped one's head in by acid-base value, temp probe, dissolved oxygen probe, bod probe realize to soda acid in city river
Degree, temperature, dissolved oxygen, bod and organic matter oxygen demand five water quality index monitoring, available for based on ammonia nitrogen
Want the water quality monitoring of pollutant city river.Immobilization bacterial strain uses separate type multiclass in biosensor in the utility model
Other bead, according to the difference of surveyed project, the microbe species utilized are also different, and ammonia is used when such as measuring ammonia-nitrogen content
Nitrogen degradation bacterium using bacillus when measuring BOD contents, accomplishes specific assay, efficiently measures purpose so as to reach, both met
It is the city river of primary pollution source to the detection requirement of " water quality pollutant oxygen demand " using ammonia nitrogen, while also meets to other dirts
Contaminate the broad spectrum activity demand of river BOD monitorings.In the utility model in biosensor microbe to screen from research object river,
It is preferable to the adaptability and mithridatism of river water body.Wireless communication module uses macro electricity DTU-H7710 data in the utility model
Zigbee module, Wi-Fi module, bluetooth module with CDMA module, GPRS module are combined and meet the offices of data by transmission unit
Domain and remote transmission requirement.
Description of the drawings
Fig. 1 is the water quality monitoring biosensor structure figure that the utility model embodiment provides.
Specific embodiment
For the ease of understanding the utility model, the utility model is more fully retouched below with reference to relevant drawings
It states.The preferred embodiment of the utility model is given in attached drawing.But the utility model can in many different forms come in fact
It is existing, however it is not limited to embodiment described herein.On the contrary, the purpose for providing these embodiments is the public affairs made to the utility model
Open the more thorough and comprehensive of content understanding.It should be noted that when an element is considered as " connection " another element, it can
To be directly to another element or may be simultaneously present centering elements.Unless otherwise defined, institute used herein
Some technical and scientific terms are identical with the normally understood meaning of the those skilled in the art for belonging to the utility model.Herein
In the utility model term used in the description be intended merely to description specific embodiment purpose, it is not intended that in
Limit the utility model.Term as used herein " and/or " including the arbitrary of one or more relevant Listed Items and
All combinations.
It please refers to shown in Fig. 1, Fig. 1 is the water quality monitoring biosensor structure figure that the utility model embodiment provides.
Water quality monitoring biosensor specifically includes in the present embodiment：Bod probe 101, acid-base value probe 102,
Temp probe 103, dissolved oxygen probe 104, signal acquisition process device 105, wireless communication module 106, constent temperature heater 107, mark
It is compacted to determine liquid case 108, liquid case to be measured 109, cleaning fluid tank 110, buffering liquid case 111, reactive tank 112, the first peristaltic pump 113, second
Dynamic pump 114, aerator 115 and exhausted bath box 116.The bod probe 101 is arranged in reactive tank 112；It is described
Acid-base value probe 102, temp probe 103, dissolved oxygen probe 104 are arranged in the liquid case to be measured 109；The bod
Probe 101, acid-base value probe 102, temp probe 103, dissolved oxygen probe 104 are connect with signal acquisition process device 105；The nothing
106 connection signal Acquisition Processor 105 of line communication module, and connect with application terminal wireless telecommunications；The bod is visited
First 101, reactive tank 112 is configured with constent temperature heater 107, and 112 lower section of the reactive tank of constent temperature heater setting in the present embodiment is used
In ensure bod probe 101, reactive tank 112 be in temperature constant state.First peristaltic pump 113 will demarcate liquid case 108,
The solution injection reactive tank 112 of liquid case 109 to be measured, cleaning fluid tank 110；Second peristaltic pump 114 will buffer the molten of liquid case 111
Liquid injects reactive tank 112；116 coupled reaction slot 112 of the aerator 115 and exhausted bath box；The bod probe 101
Biosensor is integrated with, the biosensor is immobilized microorganism bead sensing module.The micro- life of immobilization
The microbe to screen selected in object bead sensing module selects in the present embodiment from the city river using ammonia nitrogen as major pollutants
With the bacillus of orientation domestication.It should be noted that when the immobilized microorganism bead sensing module selects ammonia nitrogen degradation
During the microbial immobilized bead that bacterium makes, you can realize the measure of organic matter oxygen demand, in the present embodiment organic matter oxygen consumption
The measure of amount refers in particular to the organic based nitrogen contaminant oxygen demand based on ammonia nitrogen.It follows that it is selected for different different test events
With different biosensors, so as to reach different measure purposes.By acid-base value probe 102, temp probe 103, molten
Solve oxygen probe 104, (bod probe 101 selects different immobilized microorganism beads to perceive to bod probe 101
Module) it realizes five water quality of acid-base value in city river, temperature, dissolved oxygen, bod and organic matter oxygen demand is referred to
Target monitors.
Be integrated in the wireless communication module 106 in the present embodiment Zigbee module, Wi-Fi module, bluetooth module,
CDMA module and GPRS module.The wireless communication module 106 passes through USB2.0 interface connection signals Acquisition Processor 105.
The wireless communication module 106 is using macro electric (Shenzhen City Hongdian Technology Co., Ltd) DTU-H7710 data transmission units.
Macro electricity DTU-H7710 data transmission units use USB2.0 interfaces, and bus speed can reach 480M.In secure context, use
The three-tier system protection of industry accreditation：In original two-stage, (house dog built in software protection+CPU is protected, external hardware watchdog
Protection) on the basis of system protection, level system monitoring and protecting SWP (System Watch Protect) is increased, it solves " false
Online ", the knotty problems such as " false crash ", " working as machine ".The client that macro electricity DTU-H7710 data transmission units can provide 16M passes
Transmission of data caches, and solves the loss of data caused by network bottleneck or re-transmission, efficiency of transmission is greatly improved, reduce expenses.Pass through
Embedded ZigBee and GPRS antenna and network interface card, can support a variety of transmission modes, GPRS module, CDMA module can be by numbers
According to access telecommunications, unicom, mobile operator network, it is final to access application terminal such as mobile phone etc.；It is more in order to be adapted to
Outdoor environment, the macro electricity DTU-H7710 data transmission units also achieve bluetooth and ZigBee transport protocols, believe in mobile network
Number difference and that under conditions of WiFi network, bluetooth or ZigBee can not be transferred data to by way of multi-hop ad hoc
Data terminal.
Signal acquisition process device 105 includes the microcontroller using ARM9 embeded processors in the present embodiment.Using
The microcontroller of ARM9 embeded processors can handle various protocol data conversions at high speed, evade and being led due to CPU processing speeds are slow
The problems such as data of cause are retransmitted and are lost.It supports automatic acquisition DNS (domain name system), it is no longer necessary to human configuration inputs DNS,
Evade because the dns server of selection is abnormal, DTU equipment is caused to work as the serious phenomenon of machine.In addition, signal acquisition process device
105 are loaded with perfect ICP/IP protocol stack, and network communication performance gets a promotion.Its EMC (Electro Magnetic Compatibility) is had excellent performance, can
It is shocked by electricity and tested by electric power 3000V, using still reliable and stable under industrial circle bad environments.
Temperature has the activity of microorganism larger impact, and BOD probes and reactive tank 112 are configured with constent temperature heater 107,
To ensure that biosensor, prepare liquid and buffer solution are all under isothermal condition in measurement process.Sample introduction mainly by
First peristaltic pump 113, the second peristaltic pump 114 are completed, and measurement process includes：Cleaning solution, calibration liquid, buffer solution and prepare liquid into
Sample works.It is being cleaned, after staking-out work, the second peristaltic pump 114 is run, and buffer solution is injected in reactive tank 112, aeration dress
Operating is put, realizes and oxygen saturation state is in reactive tank 112 at a certain temperature.After 105 numerical stability of signal acquisition process device,
First peristaltic pump 113 is run, and prepare liquid is injected in reactive tank 112, is fully reacted through biosensor in popping one's head in BOD
Afterwards, signal acquisition process device 105 obtains the fast measurements of BOD.BOD probes, pH probes, T probes and DO probe gathered datas
Data information is reached into wireless communication module 106 after signal acquisition process device 105, the most data at last of wireless communication module 106
Application terminal is sent to, realizes data sharing.
BOD probes have using immobilized microorganism bead sensing module as biosensor by microorganism in utilization
The variable quantity that dissolved oxygen is consumed during machine object carrys out the concentration of organic matter in indirect determination water body.When the bio-sensing member of BOD probes
When part is in the buffer solution of oxygen saturation state, the breathing pattern of microorganism is endogenous formula breathing, and respiratory rate is constant at this time.When
Dissolved oxygen can gradually make output current reach stable when spilling into oxygen electrode.After sample to be tested is added in, it can be given birth in sample to be tested
The substance for changing degradation is utilized by the microorganism, and the breathing pattern of microorganism is converted to external source breathing at this time, is consumed big in solution
The dissolved oxygen of amount, makes the dissolved oxygen content on arrival oxygen electrode surface reduce, and is reduced so as to cause output current, which can be several
Reach constant again in minute.The difference of constant current is linear with sample concentration in a certain range obtained by front and rear measurement
Correlation, whereby can be with indirect determination BOD, that is, bod.
The utility model is suitable for city river, is with the integrated-type for independently possessing immobilization biological bead technology of preparing
Biosensor is introduced using monitoring water quality bod as monitoring main body, while according to the feature that city river water quality pollutes
This monitoring project of water quality pollutant oxygen demand, integrated temperature, acid-base value and dissolved oxygen amount to five water quality monitoring projects, reach
Basic water quality monitoring requirement.
The utility model is the biosensor designed using the more prominent city river of ammonia and nitrogen pollution as research object, is drawn
Entered " water quality pollutant oxygen demand " this monitoring project, can preferably solve the accuracy of the region water monitoring data with it is complete
Face property.BOD is to weigh water body by one of comprehensive important indicator of organic pollution level, and entire biochemical process includes two ranks
Section：First stage is carbonation stage, and second stage is the nitrification stage.BOD5 is commonly referred to as the oxygen consumption value of carbonation stage,
" water quality pollutant oxygen demand " then can be used for the overall oxygen consumption situation in two stages of reflection.For the heavier water body of ammonia and nitrogen pollution,
The oxygen demand of nitrification is more apparent during BOD is measured, and adds in microbial immobilized bead (the wide spectrum degradation of different degradation properties
Bacterium, ammonia nitrogen degradation bacterium) optionally carry out water quality monitoring project switching.
The technical solution of the utility model mesophytization oxygen demand probe 101 is integrated with biosensor, bio-sensing member
Part is immobilized microorganism bead sensing module, and microorganism is fixed on choosing card carrier by immobilized microorganism bead sensing module
On, make its highly dense and keep bioactivity, so as to fulfill the monitoring of city river mesophytization oxygen demand；The utility model leads to
Acid-base value probe 102, temp probe 103, dissolved oxygen probe 104, bod probe 101 is crossed to realize to sour in city river
Basicity, temperature, dissolved oxygen, bod and organic matter oxygen demand five water quality index monitoring, available for using ammonia nitrogen as
The water quality monitoring of major pollutants city river.Immobilization bacterial strain is more using separate type in biosensor in the utility model
Classification bead, according to the difference of surveyed project, the microbe species utilized are also different, used when such as measuring ammonia-nitrogen content
Ammonia nitrogen degradation bacterium using bacillus when measuring BOD contents, accomplishes specific assay, efficiently measures purpose so as to reach, both completely
It is enough city river that ammonia nitrogen is primary pollution source to the detection requirement of " water quality pollutant oxygen demand ", while also meets to other
The broad spectrum activity demand of polluted river channel BOD monitorings.In the utility model in biosensor microbe to screen from research object river
Road, it is preferable to the adaptability and mithridatism of river water body.Wireless communication module 106 uses macro electricity DTU- in the utility model
Zigbee module, Wi-Fi module, bluetooth module are combined completely by H7710 data transmission units with CDMA module, GPRS module
The local of sufficient data and remote transmission requirement.
Note that it above are only the preferred embodiment of the utility model and institute's application technology principle.Those skilled in the art's meeting
Understand, the utility model is not limited to specific embodiment described here, can carry out for a person skilled in the art various bright
Aobvious variation is readjusted and is substituted without departing from the scope of protection of the utility model.Therefore, although passing through above example
The utility model is described in further detail, but the utility model is not limited only to above example, is not departing from
In the case that the utility model is conceived, other more equivalent embodiments can also be included, and the scope of the utility model is by appended
Right determine.
Claims (6)
1. a kind of water quality monitoring biosensor, which is characterized in that including：Bod probe, acid-base value probe, temperature are visited
Head, dissolved oxygen probe, signal acquisition process device, wireless communication module, constent temperature heater, calibration liquid case, liquid case to be measured, cleaning solution
Case, buffering liquid case, reactive tank, the first peristaltic pump, the second peristaltic pump, aerator and exhausted bath box；The bod is visited
Head is arranged in reactive tank；The acid-base value probe, temp probe, dissolved oxygen probe are arranged in the liquid case to be measured；It is described
Bod probe, acid-base value probe, temp probe, dissolved oxygen probe and signal acquisition process device connect；The channel radio
Module connection signal Acquisition Processor is interrogated, and is connect with application terminal wireless telecommunications；The bod probe, reactive tank are matched
It is equipped with constent temperature heater；The solution of calibration liquid case, liquid case to be measured, cleaning fluid tank is injected reactive tank by first peristaltic pump；Institute
It states the second peristaltic pump and the solution for buffering liquid case is injected into reactive tank；The aerator and exhausted bath box coupled reaction slot；The life
Change oxygen demand probe and be integrated with biosensor, the biosensor is immobilized microorganism bead sensing module.
2. water quality monitoring biosensor according to claim 1, which is characterized in that the small feel of the ball of immobilized microorganism
Know the bacillus that the microorganism selected in module is orientation domestication.
3. water quality monitoring biosensor according to claim 1, which is characterized in that integrated in the wireless communication module
There are Zigbee module, Wi-Fi module, bluetooth module, CDMA module and GPRS module.
4. water quality monitoring biosensor according to claim 3, which is characterized in that the wireless communication module passes through
USB2.0 interface connection signal Acquisition Processors.
5. water quality monitoring biosensor according to claim 4, which is characterized in that the wireless communication module is using macro
Electric DTU-H7710 data transmission units.
6. the water quality monitoring biosensor according to one of claim 1 to 5, which is characterized in that at the signal acquisition
It manages device and includes the microcontroller for using ARM9 embeded processors.
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| CN201721683465.7U CN207528271U (en) | 2017-12-05 | 2017-12-05 | A kind of water quality monitoring biosensor |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111820175A (en) * | 2020-06-18 | 2020-10-27 | 安康壹加壹实业有限公司 | Circulating water aquaculture system for inland freshwater pond of penaeus vannamei boone |
| CN114609356A (en) * | 2022-04-11 | 2022-06-10 | 中山大学 | Accurate determination device and method for oxygen consumption rate of water body |
-
2017
- 2017-12-05 CN CN201721683465.7U patent/CN207528271U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111820175A (en) * | 2020-06-18 | 2020-10-27 | 安康壹加壹实业有限公司 | Circulating water aquaculture system for inland freshwater pond of penaeus vannamei boone |
| CN114609356A (en) * | 2022-04-11 | 2022-06-10 | 中山大学 | Accurate determination device and method for oxygen consumption rate of water body |
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