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CN207391446U - A kind of blake bottle - Google Patents

A kind of blake bottle Download PDF

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Publication number
CN207391446U
CN207391446U CN201721036101.XU CN201721036101U CN207391446U CN 207391446 U CN207391446 U CN 207391446U CN 201721036101 U CN201721036101 U CN 201721036101U CN 207391446 U CN207391446 U CN 207391446U
Authority
CN
China
Prior art keywords
blake bottle
side wall
bottle
fixed cover
cell culture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201721036101.XU
Other languages
Chinese (zh)
Inventor
周谊霞
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guizhou Medical University
Original Assignee
Guizhou Medical University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guizhou Medical University filed Critical Guizhou Medical University
Priority to CN201721036101.XU priority Critical patent/CN207391446U/en
Application granted granted Critical
Publication of CN207391446U publication Critical patent/CN207391446U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

The utility model discloses a kind of blake bottle, the bottle cap (2) including open top and hollow body (1) and lid conjunction body (1);Disconnected cell culture apparatus is additionally provided in body (1);The cell culture apparatus includes the side wall (3) that cross section is semi-circular shape, its bottom is horizontally arranged with circular pedestal (4), is provided with the protrusion upwardly extended without the side wall that side wall (3) surrounds along the pedestal (4).Blake bottle provided by the utility model can make cell attachment culture operation more easy.Also by the way that fixed cover (6) cell culture apparatus being sticked on body is set to be not easy to slide in blake bottle.

Description

A kind of blake bottle
Technical field
The utility model is related to biological experiment instrument field more particularly to a kind of blake bottles.
Background technology
The general Explant culture of culture of rat aorta vascular smooth muscle cells, includes the following steps:Clip rat aorta Membrane tissue in aorta vessel is shredded, membrane tissue fragment in arteries is placed in blake bottle and is pasted by blood vessel and film in extracting Wall culture.Membrane tissue fragment need to be flattened tissue pieces with tweezers in blake bottle during adhere-wall culture in arteries, and group Knitting will maintain a certain distance between fragment, it is impossible to it is packed together, it need to be with tweezers by tissue when the blake bottle used at present uses One piece one piece of fragment is put into from bottleneck in blake bottle, inconvenient.
Utility model content
In order to solve the above technical problems, the utility model provides a kind of blake bottle, so that cell attachment culture operation is more For simplicity.To achieve these goals, the utility model employs following technical solution:
A kind of blake bottle closes the bottle cap of body including open top and hollow body and lid;It is additionally provided in body Disconnected cell culture apparatus;The cell culture apparatus includes the side wall that cross section is semi-circular shape, and bottom is horizontal It is provided with circular pedestal;The protrusion upwardly extended is provided with without the side wall that side wall surrounds along the pedestal.Side wall and pedestal table Face is preferably by hydrophilic treated, in favor of cell growth.
Preferably, the body upper surface offers slot.
Preferably, the bottle cap and body are connected through a screw thread.
Preferably, the body bottom offers the outage through body bottom vertically, is provided with and arranges in outage The plug of fluid apertures tight fit.
Preferably, it is additionally provided with fixed cover in body upper surface;The fixed cover lower surface is provided with and body upper surface Slot cooperation buckle;The fixed cover lower surface is further opened with the card slot with the side wall tight fit of cell culture apparatus.
Preferably, the liquid filling hole through fixed cover is offered in the fixed cover vertically.
Preferably, at least one side outer wall of the body is without cambered.
The beneficial effects of the utility model are:Cell culture apparatus can be taken out in cell attachment culture, interior bottom Face covers fixed cover after placing tissue pieces and cell culture apparatus is sticked in blake bottle again, and operation is more easy.Also By the way that the fixed cover cell culture apparatus being sticked on body is set to be not easy to slide in blake bottle.
Description of the drawings
Fig. 1 be the utility model proposes a kind of blake bottle structure diagram.
Fig. 2 is the structure diagram of cell culture apparatus.
In figure:1- bodies, 2- bottle caps, 3- side walls, 4- pedestals, 5- slots, 6- fixed covers, 7- buckles, 8- card slots, 9- liquid feedings Hole, 10- liquid-leaking nozzles, 11- plugs.
Specific embodiment
The following is a combination of the drawings in the embodiments of the present utility model, and the technical scheme in the embodiment of the utility model is carried out It clearly and completely describes, it is clear that the described embodiments are only a part of the embodiments of the utility model rather than whole Embodiment.
A kind of blake bottle closes the bottle cap 2 of body 1, the bottle cap 2 and bottle including upper opening and hollow body 1 and lid Body 1 is connected through a screw thread;1 upper surface of body offers slot 5.Disconnected cell culture is additionally provided in body 1 Device;The cell culture apparatus includes the side wall 3 that cross section is semi-circular shape, and bottom is horizontally arranged with circular pedestal 4, The protrusion upwardly extended is provided with without the side wall that side wall 3 surrounds along the pedestal 4, side wall 3 and 4 surface of pedestal are hydrophilic treated; Cell culture apparatus further includes fixed cover 6;6 lower surface of fixed cover is provided with the buckle with the cooperation of the slot 5 of 1 upper surface of body 7;6 lower surface of fixed cover is further opened with the card slot 8 with 3 tight fit of side wall;It offers and passes through vertically in the fixed cover 6 Wear the liquid filling hole 9 of fixed cover 6.1 bottom of body offers the outage 10 through 1 bottom of body vertically, 11 in outage Inside it is provided with the plug 11 with 10 tight fit of outage.Described 1 one side outer walls of body are without cambered.
It in use, cell culture apparatus is taken out, is placed in gnotobasis, tissue pieces is flattened, be spaced apart the bottom of in On seat 4, lid closes fixed cover 6, is then sticked in cell culture apparatus on the slot 5 of 1 upper surface of body, adds in and trains from liquid filling hole 9 Nutrient solution covers bottle cap 2.When needing to discharge the liquid in cell culture apparatus, blake bottle is tilted, liquid is made to pour out into body Plug 11 is opened, you can pour out liquid in 1 bottom.When needing ventilation in blake bottle, bottle cap 2 is only opened.
The above is only the preferable specific embodiment of the utility model, but the scope of protection of the utility model is not This is confined to, in the technical scope that any one skilled in the art discloses in the utility model, according to this practicality New technical solution and its utility model design are subject to equivalent substitution or change, should all cover the protection model in the utility model Within enclosing.

Claims (7)

1. a kind of blake bottle closes the bottle cap (2) of body (1) including open top and hollow body (1) and lid;Its feature exists In:Disconnected cell culture apparatus is additionally provided in body (1);It is semicircle that the cell culture apparatus, which includes cross section, The side wall (3) of annular, bottom are horizontally arranged with circular pedestal (4), the side wall surrounded along the pedestal (4) without side wall (3) It is provided with the protrusion upwardly extended.
2. a kind of blake bottle as described in claim 1, it is characterised in that:Body (1) upper surface offers slot (5).
3. a kind of blake bottle as described in claim 1, it is characterised in that:The bottle cap (2) and body (1) are connected by screw thread It connects.
4. a kind of blake bottle as described in claim 1, it is characterised in that:Body (1) bottom is offered vertically through bottle The outage (10) of body (1) bottom, outage (10) is interior to be provided with and the plug (11) of outage (10) tight fit.
5. a kind of blake bottle as claimed in claim 2, it is characterised in that:In body (1) upper surface, returning apparatus has fixed cover (6);Fixed cover (6) lower surface is provided with the buckle (7) with the cooperation of the slot (5) of body (1) upper surface;The fixed cover (6) lower surface is further opened with the card slot (8) with the side wall (3) tight fit.
6. a kind of blake bottle as claimed in claim 5, it is characterised in that:It is offered vertically on the fixed cover (6) through solid The liquid filling hole (9) of fixed lid (6).
7. a kind of blake bottle as described in claim 1~6 is any, it is characterised in that:Outside at least one side of the body (1) Wall is without cambered.
CN201721036101.XU 2017-08-18 2017-08-18 A kind of blake bottle Expired - Fee Related CN207391446U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201721036101.XU CN207391446U (en) 2017-08-18 2017-08-18 A kind of blake bottle

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201721036101.XU CN207391446U (en) 2017-08-18 2017-08-18 A kind of blake bottle

Publications (1)

Publication Number Publication Date
CN207391446U true CN207391446U (en) 2018-05-22

Family

ID=62419230

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201721036101.XU Expired - Fee Related CN207391446U (en) 2017-08-18 2017-08-18 A kind of blake bottle

Country Status (1)

Country Link
CN (1) CN207391446U (en)

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Legal Events

Date Code Title Description
GR01 Patent grant
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20180522

Termination date: 20210818