CN204705645U - A kind of reaction cup - Google Patents
A kind of reaction cup Download PDFInfo
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- CN204705645U CN204705645U CN201520477434.0U CN201520477434U CN204705645U CN 204705645 U CN204705645 U CN 204705645U CN 201520477434 U CN201520477434 U CN 201520477434U CN 204705645 U CN204705645 U CN 204705645U
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- cup
- reaction cup
- liquid district
- reaction
- separation membrane
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- 238000004062 sedimentation Methods 0.000 claims abstract description 24
- 108090000623 proteins and genes Proteins 0.000 abstract description 7
- 102000004169 proteins and genes Human genes 0.000 abstract description 7
- 238000001514 detection method Methods 0.000 abstract description 6
- 238000004879 turbidimetry Methods 0.000 abstract description 4
- 101100327917 Caenorhabditis elegans chup-1 gene Proteins 0.000 description 16
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- 108091007433 antigens Proteins 0.000 description 4
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- 238000003786 synthesis reaction Methods 0.000 description 2
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 1
- 239000004925 Acrylic resin Substances 0.000 description 1
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- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 1
- 108010088842 Fibrinolysin Proteins 0.000 description 1
- 101000911390 Homo sapiens Coagulation factor VIII Proteins 0.000 description 1
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- CERQOIWHTDAKMF-UHFFFAOYSA-N Methacrylic acid Chemical compound CC(=C)C(O)=O CERQOIWHTDAKMF-UHFFFAOYSA-N 0.000 description 1
- VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical compound COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 description 1
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- 208000001435 Thromboembolism Diseases 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
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- 235000010489 acacia gum Nutrition 0.000 description 1
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- 229920000159 gelatin Polymers 0.000 description 1
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
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- 238000012092 latex agglutination test Methods 0.000 description 1
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- 235000010413 sodium alginate Nutrition 0.000 description 1
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- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model relates to medical device technical field, is specifically related to a kind of reaction cup detected for specific protein.This reaction cup, comprise cup (1), described cup (1) inside be provided with first liquid district (3), second liquid district (4) and be positioned between first liquid district (3) and second liquid district (4) can sedimentation separation membrane (2).Apply reaction cup of the present utility model as turbidimetry for Determination reaction unit, simplify the operation step, and for loaded down with trivial details application of sample formality is saved in clinical examination, direct injected, reacts sensitiveer.This reaction cup not only can be applied to the mensuration of DDi content, can be applied to the mensuration of other specific proteins simultaneously, bring great convenience to the detection of specific protein.
Description
Technical field
The utility model relates to technical field of medical instruments, is specifically related to a kind of reaction cup detected for specific protein.
Background technology
Plasma D-dimer content is one of catabolite of fibrinolysin hydrolytic crosslinking fibrin formation, is metabolin specific to Secondary cases fibrinolytic.The reliability index that the change of Plasma D-dimer Levels can be used as hypercoagulative state in body and differentiates as primary and Secondary cases fibrinolytic, also can be used as the observation index of thromboembolism treatment effect.Detect Plasma D-dimer Levels and have important clinical meaning for aspects such as the diagnosis of disease, efficacy determination and prognosis evaluations.
The detection method of DDi builds on nineteen eighty-three, adopts immunology principle, detectable antigens content.The analytical approach of DDi antigen is a lot, mainly contains Latex Agglutination, enzyme linked immunosorbent assay (ELISA) Micelle growth and immunoturbidimetry.Wherein latex agglutination be with special monoclonal antibody bag by latex particle, after DDi in sample combines with it, there is latex agglutination, DDi content sample can be judged from latex agglutination extension rate.ELISA method is that the first antibody of anti-DDi is coated in solid phase carrier, react with enzyme mark the second anti-D-dimer antibody again after combining with it until DDi in sodium citrate anticoagulate plasma sample, form antibody-antigene-enzyme labelled antibody sandwich complex, quantitative measurement DDi content after last plus enzyme substrate colour developing.Micelle growth is a kind of DDi detection method compared with new development, anti-D-dimer antibody is coated on the test card containing perforated membrane, plasma specimen is by testing after card well adds, DDi in film in antibody capture sample, the monoclonal antibody of anti-another epitope of DDi of colloid gold label is added after washing, be combined and aobvious redness with captured DDi molecule, its colour developing degree is directly proportional to DDi content in sample, through standard card comparison or by special detection measurement amount, can quantitative measurement DDi content.These 3 kinds of methods differ from one another, and latex agglutination test is simple and efficient, are applicable to emergency treatment inspection, but can only sxemiquantitative, and susceptibility is slightly low.ELISA method susceptibility is high, measure accurately, can quantitatively detect, but operation requirements is strict, detecting step is loaded down with trivial details time-consuming, can not meet emergency treatment inspection needs.
The Cleaning Principle of immunoturbidimetry is each emulsion particle diameter (0.1 ± 0.02) μm absorption 2 DDi specific antibodies, the diameter of this particle is less than detection light beam wavelength (540nm), thus when light beam is absorbed through only there being the light wave of trace during latex particle suspension.Determined antigen (DDi) in solution and latex particle adsorb antibodies are combined into condensate, this polymeric diameter is much larger than lambda1-wavelength, thus the absorbed amount of incident light increases greatly, and the absorbance of increase can reflect that determined antigen is at the content by sample product.Therefore, immunoturbidimetry is detected by instrument, has the advantages such as simple to operate, quick, stable, antibiont interference performance is strong, quantitatively can detect the content of DDi, be convenient to clinical observation medication, be applicable to large batch of specimen examination.
Reaction cup is widely used in the checkout procedure of biology, chemistry, medical science, uses in turbidimetry detects for the turbid analyser of match ratio.But the usual more complicated of existing turbidimetric assay reaction unit, application of sample complex operation, is not suitable for large batch of application.
Utility model content
The problem of the prior art that the utility model needs solve is: existing turbidimetric assay reaction unit is complicated, and application of sample complex operation, is not suitable for large batch of application.
In order to solve the problem, the utility model provides a kind of reaction cup.
Specifically, the utility model solves technical matters by following technical proposals:
A kind of reaction cup, comprises cup 1, described cup 1 inside be provided with first liquid district 3, second liquid district 4 and between first liquid district 3 and second liquid district 4 can sedimentation separation membrane 2.
Preferably, described can sedimentation separation membrane 2 be imitative biological membrane.
Preferably, described cup 1 and describedly can be integrated connection by sedimentation separation membrane 2.
Preferably, described cup 1 and described can sedimentation separation membrane 2 be 3D print connector.
Preferably, described first liquid district 3 and describedly can be formed as a set of cups 5 to be located at described cup 1 inner by sedimentation separation membrane 2.
Preferably, described cup 1 comprises outer wall and inwall, and described cup 1 inner diameter is greater than the diameter of described a set of cups 5.
Preferably, describedly can be positioned at apart from described cup 1 port 1/1 to four/5th place by sedimentation separation membrane 2.
Preferably, described cup 1 port is provided with sealed membrane.
Wherein, the position in described first liquid district and second liquid district can reverse, and that is, it is inner that described a set of cups can tip upside down on reaction cup cup, can sedimentation separation membrane distance cup port 1/1 to four/5th place.
Wherein, described can sedimentation separation membrane 2 can be bionical thing film, adopt hollow minute particle that macromolecular material that is natural or synthesis is encapsulated solid, liquid or gas, particle diameter 5 ~ 1000 μm, its principal feature is the physicochemical property (phase, solubleness etc.) that can change active substance; Protective substances is from the impact of environmental baseline; Shielding taste, color and smell; Reduce substance toxicity; Co ntrolled release active substance.Wherein conventional material comprises: natural macromolecular material---gelatin, Arabic gum, sodium alginate, shitosan, starch and albumen etc., semi-synthetic macromolecular material---cellulose derivative, synthesis family macromolecule material---polyglycol, polyamide and polyacrylic resin class and polyesters, PLA, polylactide and meso-lactide-glycolide copolymer etc.
The material of described cup 1 outer wall is cyclic olefine copolymer, and the material of described cup 1 inwall is cyclic olefine copolymer, and described a set of cups comprises cover wall of cup and can sedimentation separation membrane, and the material of described cover wall of cup is cyclic olefine copolymer.
Described reaction cup can adopt 3D printing technique to make, and adopts 3D printing technique to realize cup and can the integration of sedimentation separation membrane, can for by the loading of Dual-layer liquid solution of sedimentation separation membrane separation bringing great convenience; Also can by by described first liquid district with describedly can be formed as a set of cups to be located at cup 1 inner by sedimentation separation membrane, wherein, the position in described first liquid district and second liquid district can reverse, and that is, it is inner that described a set of cups can tip upside down on reaction cup cup; Can also by integrated mould by cup and can sedimentation separation membrane compressed together.
The beneficial effects of the utility model are: apply reaction cup of the present utility model as turbidimetry for Determination reaction unit, simplify the operation step, and for loaded down with trivial details application of sample formality is saved in clinical examination, direct injected, reacts sensitiveer.This reaction cup not only can be applied to the mensuration of DDi content, can be applied to the mensuration of other specific proteins simultaneously, bring great convenience to the detection of specific protein.
Below in conjunction with accompanying drawing and each embodiment, the utility model and Advantageous Effects thereof are described in detail, wherein:
Accompanying drawing explanation
Fig. 1 is the structural representation of the reaction cup of the utility model embodiment one.
Fig. 2 is the structural representation of the reaction cup of the utility model embodiment two.
Wherein, 1 is cup, and 2 is can sedimentation separation membrane, and 3 is first liquid district, and 4 is second liquid district, and 5 is a set of cups.
Embodiment
As mentioned above, the purpose of this utility model is: provide a kind of reaction cup being used as turbidimetric assay, simplify the operation step, saves loaded down with trivial details application of sample program, makes mensuration sensitiveer simultaneously.
Below in conjunction with the drawings and specific embodiments, the utility model is described in further detail.
Embodiment one
Fig. 1 is the structural representation of the reaction cup of the utility model embodiment one, comprises cup 1, described cup 1 inside be provided with first liquid district 3, second liquid district 4 and between first liquid district 3 and second liquid district 4 can sedimentation separation membrane 2.
Described cup 1 is made up of outer wall and inwall.The material of described inside and outside wall is cyclic olefine copolymer.This material can be applied to turbidimetry for Determination specific protein content, does not affect transmission and the scattering process of light.
Described can sedimentation separation membrane 2 be imitative biological membrane, and imitative biological membrane adopts the method for this field routine to prepare by methacrylic acid one Lu one hydroxyl ethyl ester (HEMA) and methyl methacrylate (MMA).
Reaction cup is realized by 3D printing technique, first print cup, then as required a kind of solution is loaded bottom of cup, continue to print, biological membrane is compressed in the middle of cup, wherein the link position of imitative biological membrane and cup is positioned at distance cup port 1/4th place, and then has then printed cup, loads another kind of solution simultaneously.Utilize double-layer sterile vacuum technique in cup 1 upper end simultaneously, adopt the sealing of aluminium foil material.Adopt 3D printing technique can the integration of realization response cup.This reaction cup is not suitable for the liquid stored of putting together before being particularly suitable for reaction, imitate biological membrane during reaction and have settlement action, effect is separated by reactant liquor, after reaction starts, at the bottom of cup body rotation effect can be sunk to glass, do not affect transmission and the scattering process of light.
Tear sealed membrane, add sample solution, vacuum expendable pattern, imitative biological membrane starts along with the stirring of specific protein analyser, reacts and starts, through certain reaction time, finally print testing result.
Embodiment two
Embodiment two provides a kind of reaction cup being nested with a set of cups, and as shown in Figure 2, wherein 1 is cup, and 2 is can sedimentation separation membrane, and 3 is first liquid district, and 4 is second liquid district, and 5 is a set of cups.
Embodiment two is with the difference of embodiment one, embodiment two is embedded with a set of cups 5 in cup inside, and be imitative biological membrane bottom described a set of cups 5, cover wall of cup adopts to be made with the material that reaction cup cup inside and outside wall is identical, a set of cups rim of a cup hangs on reaction cup port, sealing.Described imitative biological membrane is positioned at apart from described cup port 1/4th place.
Embodiment three
Embodiment three is with the difference of embodiment one, cup in embodiment three adopts integrated mould to make and obtains, cup upper and lower is made respectively by integrated mould, the ratio of the height of cup upper and lower is 1:4, then a kind of solution is loaded in cup bottom, between cup upper and lower, add imitative biological membrane simultaneously, pass through vacuum pressed, make thoroughly to bond between cup top, bottom and imitative biological membrane, load another kind of solution on the cup top prepared, sealing simultaneously.
The foregoing is only the utility model preferred embodiment, and be not used in limitation the utility model, all make within spirit of the present utility model and principle amendment, equivalent to replace and improvement etc., within the protection domain all needing to be included in utility model.
Claims (8)
1. a reaction cup, comprise cup (1), it is characterized in that: described cup (1) inside be provided with first liquid district (3), second liquid district (4) and be positioned between first liquid district (3) and second liquid district (4) can sedimentation separation membrane (2).
2. reaction cup according to claim 1, is characterized in that, described can sedimentation separation membrane (2) be imitative biological membrane.
3. reaction cup according to claim 1, is characterized in that, described cup (1) and describedly can be integrated connection by sedimentation separation membrane (2).
4. reaction cup according to claim 1, is characterized in that, described cup (1) and described can sedimentation separation membrane (2) be 3D print connector.
5. reaction cup according to claim 1, is characterized in that, described first liquid district (3) and describedly can be formed as a set of cups (5) to be located at described cup (1) inner by sedimentation separation membrane (2).
6. reaction cup according to claim 5, is characterized in that, described cup (1) comprises outer wall and inwall, and described cup (1) inner diameter is greater than the diameter of described a set of cups (5).
7. according to the arbitrary described reaction cup of claim 1-6, it is characterized in that, describedly can be positioned at apart from described cup (1) port 1/1 to four/5th place by sedimentation separation membrane (2).
8., according to the arbitrary described reaction cup of claim 1-6, it is characterized in that, described cup (1) port is provided with sealed membrane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520477434.0U CN204705645U (en) | 2015-07-03 | 2015-07-03 | A kind of reaction cup |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520477434.0U CN204705645U (en) | 2015-07-03 | 2015-07-03 | A kind of reaction cup |
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| Publication Number | Publication Date |
|---|---|
| CN204705645U true CN204705645U (en) | 2015-10-14 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520477434.0U Expired - Fee Related CN204705645U (en) | 2015-07-03 | 2015-07-03 | A kind of reaction cup |
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| Country | Link |
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| CN (1) | CN204705645U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105699667A (en) * | 2016-01-26 | 2016-06-22 | 北京中科圆融生物科技发展有限公司 | Bacterial magnetic particle-red cell membrane composite particles and clinical application thereof |
-
2015
- 2015-07-03 CN CN201520477434.0U patent/CN204705645U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105699667A (en) * | 2016-01-26 | 2016-06-22 | 北京中科圆融生物科技发展有限公司 | Bacterial magnetic particle-red cell membrane composite particles and clinical application thereof |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20151014 |