CN1825121A - Plastic chip - Google Patents
Plastic chip Download PDFInfo
- Publication number
- CN1825121A CN1825121A CN 200510024039 CN200510024039A CN1825121A CN 1825121 A CN1825121 A CN 1825121A CN 200510024039 CN200510024039 CN 200510024039 CN 200510024039 A CN200510024039 A CN 200510024039A CN 1825121 A CN1825121 A CN 1825121A
- Authority
- CN
- China
- Prior art keywords
- lower clamp
- chip bar
- reaction plate
- chip
- hole
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000004033 plastic Substances 0.000 title claims abstract description 13
- 229920003023 plastic Polymers 0.000 title claims abstract description 13
- 238000006243 chemical reaction Methods 0.000 claims abstract description 21
- 238000000018 DNA microarray Methods 0.000 claims description 20
- 239000000463 material Substances 0.000 claims description 13
- 238000001514 detection method Methods 0.000 claims description 9
- 102000004169 proteins and genes Human genes 0.000 claims description 5
- 108090000623 proteins and genes Proteins 0.000 claims description 5
- 239000004793 Polystyrene Substances 0.000 claims description 2
- 238000005842 biochemical reaction Methods 0.000 claims description 2
- 238000009396 hybridization Methods 0.000 claims description 2
- 230000036039 immunity Effects 0.000 claims description 2
- 239000002184 metal Substances 0.000 claims description 2
- 229920002223 polystyrene Polymers 0.000 claims description 2
- 239000000376 reactant Substances 0.000 claims description 2
- 239000005060 rubber Substances 0.000 claims description 2
- 210000002966 serum Anatomy 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 6
- 230000000875 corresponding effect Effects 0.000 description 5
- 239000004677 Nylon Substances 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 229920001778 nylon Polymers 0.000 description 3
- 239000007790 solid phase Substances 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 238000002493 microarray Methods 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 230000000274 adsorptive effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N ferric oxide Chemical compound O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Images
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to a plastic chip that is made up of upper clip tool, down clip tool, chip stick, and reaction board. The clip stick uses plastic as raw material and could be uninstalled and combined freely. After upper and down clip tools fixing the chip stick, using high speed spot sample device constructs biology sample array on the chip stick and buckling into the reacting board that contains tested blood serum or sample to take reaction. The invention is convenience to make and use, and is low cost.
Description
Technical field
The present invention relates to biochip technology and external clinical diagnosis detection technique field, more precisely, relate to a kind of biochip that is suitable for many indexs, the parallel detection of many person-portions.
Background technology
The notion of biochip comes from semiconductor, computer chip, mainly is meant the dna microarray, oligonucleotide microarray and the protein microarray that are coated on the solid phase carrier.At present common biochip is divided into three major types: i.e. genetic chip, protein chip, chip lab.The biochip principal feature is high flux, microminiaturization and robotization, can obtain the biological information in the sample rapidly and accurately in a large amount of biomolecule of very short time inner analysis, and detection efficiency is hundreds and thousands of times of traditional detection means.The solid phase carrier that biochip is commonly used both at home and abroad has slide and nylon membrane two big classes at present.Slide is mainly used in genetic chip, has cheapness, detects the low advantage of background, but need surface of glass slide is carried out complex chemical treatment before point sample, is difficult to processing, frangible.Though nylon membrane to the biomolecule excellent adsorption, helps keeping the biomolecule natural activity, common background signal value height, at present main dependence on import costs an arm and a leg.
Therefore it is a kind of with the biochip of low, cheap, the wield material of background signal value as solid phase carrier that this area presses for exploitation.Plastics are materials of a kind of desirable preparation biochip.Plastics are moderate to the adsorptive power of albumen, significantly overcome the high shortcoming of nylon membrane background signal value.Plastics also are processed into different shape, non-friable easily, and are cheap, and only need simple process just can use before point sample.Therefore our a kind of plastic chip that has been developing material with plastics, except that possessing above-mentioned making, advantage such as easy to use, the chip bar can also freely be dismantled, make up, and makes that the index of clinical detection and people's umber can independent assortments, more adapts to actual needs of clinical detection.
Summary of the invention
The objective of the invention is at the problems referred to above, a kind of clinical many indexs, many person-portions parallelization disease detection biochip of can be applicable to is provided.A kind of low background, low cost, chip system easy and simple to handle are provided.
The invention provides a kind of plastic biochips, it is characterized in that, it contains following assembly:
---chip bar (1), the lobed horizontal end face of described chip bar and be convenient to the fixing base of upper and lower clamp;
---go up anchor clamps (2), described upward anchor clamps are that one or four limits have the frame bottom seal box, and four limits have projection or the hole slot of being convenient to the lower clamp interlock simultaneously;
---lower clamp (3), described lower clamp have and the corresponding hole of the chip horizontal end face of projection, and four limits have projection or the hole slot of being convenient to last anchor clamps interlock simultaneously;
---reaction plate (4), described reaction plate have and the corresponding seal bore of chip bar projection end face, can add reactant liquor in the hole and be convenient to chip bar end face contact carrying out biochemical reaction;
Wherein, each assembly is separated from each other, and when being assembled together, and described chip bar (1), each position of going up lower clamp (2,3) and reaction plate (4) are corresponding mutually, in conjunction with tight.
In a preference, the position of chip bar (1), the shape that goes up anchor clamps (2), lower clamp (3), reaction plate (4), size, hole array all is corresponding one by one, can fit like a glove.
In another preference, the material selection polystyrene material of chip bar (1) or other have the material to biologically active sample (DNA, protein etc.) adsorbability.
In another preference, chip bar (1) can conveniently be dismantled the needed detection person-portion of independent assortment from last lower clamp (2,3).
In another preference, lower clamp (3) is 48,96,128,384 with the number in the pairing hole of reaction plate (4).
In another preference, described chip bar (1) shape, big or small consistent with the size shape in lower clamp (3) hole, external form is rectangle, hexagon, octagon or circle substantially.
In another preference, described chip bar (1) can tip upside down on reaction plate (4) lining and carry out various reactions such as immunity, hybridization, biochemistry after finishing with last lower clamp (2,3) assembly.
In another preference, described upper and lower anchor clamps (2,3) are selected from down group with the material of reaction plate (4): plastics, rubber or metal and all operable materials.
Description of drawings
Accompanying drawing is the exploded view of a kind of example of the present invention.
Specific embodiments
As shown in the figure, biochip among the present invention comprises anchor clamps 2, lower clamp 3, chip bar 1,4 four assemblies of reaction plate, and each assembly is separate, in addition, also contain optional fixed form (projection or hole slot etc.), be used for together the said modules fixed combination.
Below in conjunction with specific embodiment, further set forth the present invention.
Embodiment
(1) chip bar 1 is put into the lower clamp corresponding aperture, upper and lower anchor clamps 2,3 are closed tightly fixed core slip 1.
(2) make up biological sample (DNA, protein etc.) dot matrix in chip bar 1 upper surface with the high speed point sample instrument.
(3) in reaction plate 4 holes, add blood serum sample to be detected and correlated response liquid successively, go into reaction plate and hatch, react closing tight last lower clamp and chip bar assembly back-off.
(4) mode of washing washes routinely.
Upper and lower anchor clamps 2,3 that (5) will wash and chip bar 1 assembly are put into detector and are detected.
Should be understood that those skilled in the art can make various changes or modifications the present invention after having read above-mentioned teachings of the present invention, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Claims (8)
1. a plastic biochips is characterized in that, it contains following assembly:
-chip bar (1), the lobed horizontal end face of described chip bar and be convenient to the fixing base of upper and lower clamp;
---go up anchor clamps (2), described upward anchor clamps are that one or four limits have the frame bottom seal box, and four limits have projection or the hole slot of being convenient to the lower clamp interlock simultaneously;
---lower clamp (3), described lower clamp have and the corresponding hole of the chip horizontal end face of projection, and four limits have projection or the hole slot of being convenient to last anchor clamps interlock simultaneously;
---reaction plate (4), described reaction plate have and the corresponding seal bore of chip bar projection end face, can add reactant liquor in the hole and be convenient to chip bar end face contact carrying out biochemical reaction;
Wherein, each assembly is separated from each other, and when being assembled together, and described chip bar (1), each position of going up lower clamp (2,3) and reaction plate (4) are corresponding mutually, in conjunction with tight.
2. biochip according to claim 1 is characterized in that, the position of chip bar (1), the shape that goes up anchor clamps (2), lower clamp (3), reaction plate (4), size, hole array all is corresponding one by one, can fit like a glove.
3. biochip according to claim 1 is characterized in that, the material selection polystyrene material of chip bar (1) or other have the material to biologically active sample (DNA, protein etc.) adsorbability.
4. biochip according to claim 1 is characterized in that, chip bar (1) can conveniently be dismantled the needed detection person-portion of independent assortment from last lower clamp (2,3).
5. biochip according to claim 1 is characterized in that, lower clamp (3) is 48,96,128,384 with the number in the pairing hole of reaction plate (4).
6. biochip according to claim 1 is characterized in that, described chip bar (1) shape, big or small consistent with the size shape in lower clamp (3) hole, and external form is rectangle, hexagon, octagon or circle substantially.
7. biochip according to claim 1 is characterized in that, described chip bar (1) can tip upside down on reaction plate (4) lining and carry out various reactions such as immunity, hybridization, biochemistry after finishing with last lower clamp (2,3) assembly.
8. biochip according to claim 1 is characterized in that, described upper and lower anchor clamps (2,3) are selected from down group with the material of reaction plate (4): plastics, rubber or metal and all operable materials.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2005100240398A CN1825121B (en) | 2005-02-24 | 2005-02-24 | Plastic chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2005100240398A CN1825121B (en) | 2005-02-24 | 2005-02-24 | Plastic chip |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1825121A true CN1825121A (en) | 2006-08-30 |
| CN1825121B CN1825121B (en) | 2011-06-08 |
Family
ID=36935888
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2005100240398A Active CN1825121B (en) | 2005-02-24 | 2005-02-24 | Plastic chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1825121B (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102226147A (en) * | 2011-05-30 | 2011-10-26 | 苏州偲聚生物材料有限公司 | Biochip incubation reactor |
| CN102323429A (en) * | 2011-08-17 | 2012-01-18 | 上海裕隆生物科技有限公司 | Protein membrane chip |
| CN102507955A (en) * | 2011-10-20 | 2012-06-20 | 上海裕隆生物科技有限公司 | Protein film chip reaction bar |
| CN102507954A (en) * | 2011-10-20 | 2012-06-20 | 上海裕隆生物科技有限公司 | Chip reaction strip |
| CN102507956A (en) * | 2011-10-20 | 2012-06-20 | 上海裕隆生物科技有限公司 | Detachable protein film chip |
| CN106153939A (en) * | 2014-10-17 | 2016-11-23 | 广州瑞博奥生物科技有限公司 | A kind of antibody chip test kit for detecting antiapoptotic factors |
| CN107340388A (en) * | 2017-06-14 | 2017-11-10 | 杨华卫 | A kind of biomolecule detecting method |
| CN107389933A (en) * | 2017-06-14 | 2017-11-24 | 杨华卫 | A kind of biochip |
| CN107636463A (en) * | 2015-05-26 | 2018-01-26 | 烟台载通生物技术有限公司 | Multi-Unit Plate For Immunoblot Analysis |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2497313Y (en) * | 2001-08-31 | 2002-06-26 | 上海数康生物科技有限公司 | Protein chip |
| CN1186635C (en) * | 2002-03-29 | 2005-01-26 | 成都夸常科技有限公司 | Biological chip capable of being disassembled and assembled |
| CN2638069Y (en) * | 2003-07-21 | 2004-09-01 | 中国人民解放军军事医学科学院放射医学研究所 | Biological chip reaction box |
-
2005
- 2005-02-24 CN CN2005100240398A patent/CN1825121B/en active Active
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102226147A (en) * | 2011-05-30 | 2011-10-26 | 苏州偲聚生物材料有限公司 | Biochip incubation reactor |
| CN102226147B (en) * | 2011-05-30 | 2013-01-23 | 苏州偲聚生物材料有限公司 | Biochip incubation reactor |
| CN102323429A (en) * | 2011-08-17 | 2012-01-18 | 上海裕隆生物科技有限公司 | Protein membrane chip |
| CN102507955A (en) * | 2011-10-20 | 2012-06-20 | 上海裕隆生物科技有限公司 | Protein film chip reaction bar |
| CN102507954A (en) * | 2011-10-20 | 2012-06-20 | 上海裕隆生物科技有限公司 | Chip reaction strip |
| CN102507956A (en) * | 2011-10-20 | 2012-06-20 | 上海裕隆生物科技有限公司 | Detachable protein film chip |
| CN106153939A (en) * | 2014-10-17 | 2016-11-23 | 广州瑞博奥生物科技有限公司 | A kind of antibody chip test kit for detecting antiapoptotic factors |
| CN107636463A (en) * | 2015-05-26 | 2018-01-26 | 烟台载通生物技术有限公司 | Multi-Unit Plate For Immunoblot Analysis |
| CN107340388A (en) * | 2017-06-14 | 2017-11-10 | 杨华卫 | A kind of biomolecule detecting method |
| CN107389933A (en) * | 2017-06-14 | 2017-11-24 | 杨华卫 | A kind of biochip |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1825121B (en) | 2011-06-08 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| ASS | Succession or assignment of patent right |
Owner name: SHANGHAI YULONG BIOLOGICAL SCIENCE AND TECHNOLOGY Free format text: FORMER OWNER: MU HAIDONG Effective date: 20101025 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20101025 Address after: 200233 building 50, building 1089, 4 North Qinzhou Road, Shanghai Applicant after: Shanghai Yulong Biological Science And Technology Co., Ltd. Address before: 200233 building 50, building 1089, 4 North Qinzhou Road, Shanghai Applicant before: Mu Haidong |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Qufu Yulong Biological Technology Co., Ltd. Assignor: Shanghai Yulong Biological Science And Technology Co., Ltd. Contract record no.: 2012310000043 Denomination of invention: Plastic chip Granted publication date: 20110608 License type: Exclusive License Open date: 20060830 Record date: 20120410 |