CN1806621A - Liquid milk - Google Patents

Abstract

The invention discloses a liquid milk comprising the following constituents (by weight portion): milk 50-90, IGF-1 0.25-0.50, emulsification thickening agent 0.1-0.3, and water 8.20-49.6.

Description

A kind of liquid milk

Technical field

The present invention relates to the beverage of suckling, particularly relate to the liquid milk of a kind of IGF-1 of containing.

Background technology

As everyone knows, calcium is the maximum a kind of mineral element of adult's in-vivo content, and becoming human body to include the calcium total amount is 1200～1300 grams, and wherein 99% is present in bone and the tooth.Calcium all plays an important role for the contraction of skeleton and tooth development, blood clotting, cardiac muscle and skeletal muscle, the adjusting of nerve cell function.

But at present China resident generally is in " calcifame statural " state, causes that fetus or upgrowth and development of children are slow, bone and deformity teeth, and the elderly's skeleton bulking and brothers shrink disease.And calcium deficiency and the crowd that relates to all ages and classes, it is significant in China therefore to replenish the calcium.For the most directly influence that calcium deficiency caused is that osteoporosis appears in human body, along with the increase at age, and the bone-loss aggravation, and health weakens calcareous absorption is also corresponding, thereby cause human body negative calcium balance to occur.The main cause that negative calcium balance and calcium deficiency appear in human body has the following aspects:

1) dietary factor: long-term calcareous intake deficiency also can cause forming in the body negative calcium balance;

2) inherent cause: the ill probability of person also can increase greatly the family history;

3) hormone factor: calcareous absorption and bone metabolism are subjected to multiple hormonal effects, as calcium-regulating hormone etc.;

4) medicine factor: some medicines and chemicotherapy method also can cause osteoporosis;

5) physiologic factor: for example climacteric women and old man;

6) bad life habits: excessive drinking and smoking; Drink the beverage that contains caffeine; The picked-up excess protein also can increase calcareous loss; China's meals are many based on cereal, vegetables, and phytic acid is too much in the cereal, also contain in some fruit tannin, phytic acid, oxalic acid, tannin in enteron aisle easily and the calcium ion in the food be combined into insoluble calcium salt, influence the absorption of calcium;

7) lack exercise: lack exercise and to quicken the loss of sclerotin;

8) age increases: the bone-loss aggravation, health weakens calcareous absorbability is also corresponding.

Above factor all is to cause the principal element of human body osteohalsiteresis, drinks the symptom that a certain amount of milk product can change negative calcium balance.

At present, the nutritional labeling of the sterile milk that circulates in the market is enumerated as follows:

Calcium (Ca) content is at 50mg--100mg/110g;

Protein content is at 1.0g-2.9g/100g;

Soluble solid content is at 11.6g-12.4g/100g;

Fat content is at 1.0g-3.3g/100g;

Lactose content is at 1.0g-4.7g/100g.

More than to have direct acting to skeleton in these nutritional labelings be what of calcium, especially calcium content in the product.But simple calcium absorptivity is low by increasing the content of calcium in the milk, and effect is undesirable.

Summary of the invention

The purpose of this invention is to provide a kind of liquid milk that can promote that human body absorbs calcium.

Liquid milk provided by the present invention, contain the component of following weight portion:

Milk 50-90;

IGF-1 0.25-0.50；

Emulsifing thickener 0.10-0.30;

Water 8.20-49.6.

In order to improve the nutritive value of liquid milk, also contain the component of one or more following weight portions in the liquid milk:

Vitamin V _D30.1-0.3;

CPP 0.1-0.3;

Milk calcium 0.1-0.3.

In the present invention, can adopt the employed emulsifing thickener of existing sterilizing flavouring breast, comprise molecule distillating monoglyceride, polyglycerol ester, xanthans and carragheen, its weight fraction is such as following:

Molecule distillating monoglyceride 1,

Polyglycerol ester 0.2-0.4,

Carragheen 0.1-0.2,

Xanthans 0.2-0.4.

The present invention adds a certain amount of IGF-1 and cholecalciferol, CPP, milk calcium in the milk after pasteurize or ultra high temperature short time sterilization, thereby when realizing consumer's milk drink, existing abundant trophism has special health-care effect again, mainly is the stalwartness that is beneficial to bone.The results showed that milk, IGF-1, cholecalciferol, CPP, milk calcium mix the effect of using and obviously be better than the effect that each composition uses separately.This product especially is in the bone sub-health population to various consumer groups certain effect, nutritious, and behind pasteurize or UHTS, long shelf-life is better than fresh milk, delicate mouthfeel, the heat-treated milk of taste sweet-smelling.

The present invention is by adding IGF-1 in milk, substantially can satisfy the psychological need of human body, can promote the absorption of human body to calcium, simultaneously, the physiologically active of nutritional labeling can also keep in preparation process in the milk, be not destroyed, the crowd who is widely used in children, teenager, adult, pregnant woman, the elderly and is in all age group of bone inferior health, the crowd to calcareous absorption difference also has certain effect simultaneously.

The specific embodiment

In embodiments of the present invention, raw materials used source is as follows:

Milk: the inferior pasture of Inner Mongol Mongolia Ox dairy industry (group) company Australia;

IGF-1: Shanghai system circle food technology Co., Ltd;

Stabilizing agent: green science and technology Co., Ltd of Beijing University;

Cholecalciferol: limited company is encouraged in Shanghai;

CPP (CPP): my Food Co., Ltd of Denmark;

Milk calcium: my Food Co., Ltd of Denmark.

Embodiment 1, contain the preparation of IGF-1 liquid milk

Prescription (weight percentage):

Raw material ingredient requirement addition

Butterfat 〉=3.3%, protein 〉=2.9% 55%

Non-fat solid 〉=8.1%

Emulsifing thickener 0.2%

Water 44.45%

IGF-1 0.25％

Vitamin D ₃0.1%

Wherein, the prescription of emulsifing thickener (weight portion) is: 1 part of molecule distillating monoglyceride, 0.4 part of polyglycerol ester, 0.1 part of carragheen, 0.2 part of xanthans.

The preparation process technological operation is as follows:

1. milk produced technology: milk carries out requiring every index to meet production requirement after the standardization, and fat is 3.4%-4.1% after the standardization, and protein is 2.95%-3.45%.Raw material milk requires refrigeration below 4 ℃.The former milk that uses

Specific requirement is as follows:

A. organoleptic indicator:

Color and luster: be the milky of uniformity or be with yellow slightly.

Grow smell: have the intrinsic fragrance of fresh milk, do not have other peculiar smell.

Structural state: be uniform colloidal state fluid, do not have precipitation, no grumeleuse, the visible impurity of no naked eyes and other foreign matter.

B. physical and chemical index:

Relative density d ²⁰ ₄1.028-1.032

Fat, % 〉=3.2

Protein, % 〉=2.90

Non-fat solid, % 〉=8.3

Acidity, ° T≤18.0

The impurity degree, mg/kg≤4.0

C. sanitary index:

Mercury (in Hg), mg/kg≤0.01

Arsenic (in As), mg/kg≤0.2

Plumbous (in Pb), mg/kg≤0.05

Chromium is (with Cr ⁶⁺Meter), mg/kg≤0.3

Nitrate is (with NaNO ₃Meter), mg/kg≤8.0

Nitrite is (with NaNO ₂Meter), mg/kg≤0.2

BHC, mg/kg≤0.05

DDT, mg/kg≤0.02

Aflatoxin M ₁, μ g/kg≤0.2

Antibiotic must not detect

D. microbiological indicator:

Total plate count, cfu/ml≤500000

E. mingle project:

Must not in fresh cow's milk, mix non-milk components such as alkaline matter, starch, salt, sucrose

2. prepare burden:

2.1. the milk that adds 600 kilograms in sugar dissolving kettle slowly is warmed up to 40 ℃-45 ℃, and emulsifing thickener is added, and keeps the raw material unanimity that is uniformly dispersed, and is warmed up to 70 ℃ then, keeps 15 minutes-20 minutes under this temperature, and raw material is fully dissolved.Raw material with before cold milk mixes with vitamin V _D3Add in 70 ℃ the warm milk, squeeze into blending tank after disperseing to cool to 20 ℃ later in 5 minutes.Mixed 15 minutes.

2.2.IGF-1 dissolving: will change batch can and carry out pressuresteam sterilization in advance, in the cooled aseptic jar of sterilization, squeeze into an amount of quality raw materials milk (requiring total number of bacteria) then less than 300,000/milliliter, in the interlayer with steam feedingization batch can, milk in the feasibleization batch can is warmed up to 70 ℃, and under this temperature, kept 5 minutes, reach under the aseptic condition, cool to 40 ℃, open a jar mouth, IGF-1 is added, kept 15 minutes, behind the no macroscopic particle of eyepiece observation, from the sampling of sterile sampling valve port, under magnifying glass, observe till the no obvious visible particle then.Cool to 15 ℃ then, this material is all squeezed into blending tank.Mixed 15 minutes.

3. constant volume: raw material residual in raw material in the sugar dissolving kettle and the pipeline is all headed in the blending tank with a spot of raw material milk.

4. preheating: be preheating to 60 ℃-75 ℃ by the hot plate mixed material of changing commanders.

5. homogeneous: homogenization pressure is 22-25MPa, and the one-level homogenization pressure is 12-14MPa, and double-stage homogenization pressure is 8-10MPa.The homogenizer feed pressure is 0.20-0.40MPa, and homogenizing temperature is 55 ℃-75 ℃.

6. pre-sterilizing: sterilization temperature should be controlled at 85 ℃-90 ℃/15 seconds.

7. flash concentration: the flash tank vacuum degree control is at-0.055Mpa--0.085Mpa, going out flash temperature is 30 ℃-80 ℃, advancing flash temperature is 65 ℃-90 ℃, the clear water temperature is not higher than 15 ℃, milk is squeezed into coldplate after the flash distillation, inlet temperature is 43 ℃-50 ℃, and outlet temperature is 25 ℃-30 ℃, and the frozen water temperature is 1 ℃-4 ℃.

8. cooling: the semi-finished product temperature of advancing to raise is controlled at 25 ℃-30 ℃, cools to 4 ℃-8 ℃ by the milk of cold drawing after with pre-sterilizing.The semi-finished product resting period must not be above 12 hours.

9. semi-finished product are stored: the milk after pre-sterilizing is handled is put into blending tank, stirs 20 minutes, guarantees the material uniformity.And check every physical and chemical index of product, mainly detect the content of fat, protein, dry, acidity, PH numerical value and the IGF-1 of this product.

10. ultra high temperature short time sterilization: the inlet temperature of material is controlled in 25 ℃, and sterilising temp is 137 ℃ ± 2 ℃/4 seconds.

11. sterile filling obtains product

Assay is as follows:

The project indicator

Total solid 7.5%

Lactose 2.4%

Protein 1.06%

Fat 1.54%

IGF-1 5.7mg/100g

Vitamin D ₃60IU/100g

Calcium 54.0mg/100g

Potassium 61.0mg/100g

Phosphorus 43.0mg/100g

Embodiment 2, contain the preparation of IGF-1 liquid milk

Prescription (weight percentage):

Raw material ingredient requirement addition

Butterfat 〉=3.3%, protein 〉=2.9% 85%

Non-fat solid 〉=8.1%

Emulsifing thickener 0.2%

Water 14.15%

IGF-1 0.50％

Vitamin D ₃0.15%

Wherein, the prescription of emulsifing thickener is (weight portion): 1 part of molecule distillating monoglyceride, 0.3 part of polyglycerol ester, 0.1 part of carragheen, 0.4 part of xanthans.

Preparation process is identical with embodiment 1, and the result is as follows in the examination and test of products:

The project indicator

Total solid 11.5%

Lactose 4.4%

Protein 2.76%

Fat 3.24%

IGF-1 12.8mg/100g

Vitamin D ₃84IU/100g

Calcium 112.0mg/100g

Potassium 124.0mg/100g

Phosphorus 53.0mg/100g

Embodiment 3, contain the preparation of IGF-1 liquid milk

Prescription (weight percentage):

Raw material ingredient requirement addition

Butterfat 〉=3.3%, protein 〉=2.9% 55%

Non-fat solid 〉=8.1%

Emulsifing thickener 0.2%

Water 44.45%

IGF-1 0.25％

CPP 0.1％

Wherein, the prescription of emulsifing thickener is (weight portion): 1 part of molecule distillating monoglyceride, 0.2 part of polyglycerol ester, 0.1 part of carragheen, 0.4 part of xanthans.

Preparation process is identical with embodiment 1, and assay is as follows:

The project indicator

Total solid 7.5%

Lactose 2.4%

Protein 1.13%

Fat 1.34%

IGF-1 5.65mg/100g

Vitamin D ₃76IU/100g

Calcium 52.0mg/100g

Potassium 66.0mg/100g

Phosphorus 49.0mg/100g

Embodiment 4, contain the preparation of IGF-1 liquid milk

Prescription (weight percentage):

Raw material ingredient requirement addition

Butterfat 〉=3.3%, protein 〉=2.9% 90%

Non-fat solid 〉=8.1%

Emulsifing thickener 0.2%

Water 9.0%

IGF-1 0.50％

CPP 0.3％

Wherein, the prescription of emulsifing thickener is (weight portion): 1 part of molecule distillating monoglyceride, 0.2 part of polyglycerol ester, 0.2 part of carragheen, 0.2 part of xanthans.

Preparation process is identical with embodiment 1, and assay is as follows:

Project	Index
		Total solid	12.65％
Lactose	4.6％
		Protein	2.86％
Fat	3.44％
		IGF-1	14.8mg/100g
Vitamin D 3	104IU/100g
		Calcium	112.0mg/100g
Potassium	121.0mg/100g
		Phosphorus	53.0mg/100g

Embodiment 5, contain the preparation of IGF-1 liquid milk

Prescription (weight percentage):

Raw material	Ingredient requirement	Addition
			Milk	Fat 〉=3.3%, protein 〉=2.9% non-fat solid 〉=8.1%	55％
Emulsifing thickener		0.2％
			Water		44.25％
IGF-1		0.25％
			Cholecalciferol		0.1％

Milk calcium		0.1％
			CPP		0.1％

Wherein, the prescription of emulsifing thickener is (weight portion): 1 part of molecule distillating monoglyceride, 0.4 part of polyglycerol ester, 0.1 part of carragheen, 0.2 part of xanthans.

Preparation process is identical with embodiment 1, and the result is as follows in the examination and test of products:

Project	Index
		Total solid	7.5％
Lactose	2.4％
		Protein	1.16％
Fat	1.72％
		IGF-1	6.65mg/100g
Vitamin D 3	86IU/100g
		Calcium	117.0mg/100g
Potassium	68.0mg/100g
		Phosphorus	54.0mg/100g

Embodiment 6, contain the preparation of IGF-1 liquid milk

Prescription (weight percentage):

Raw material	Ingredient requirement	Addition
			Milk	Fat 〉=3.3%, protein 〉=2.9% non-fat solid 〉=8.1%	90％
Emulsifing thickener		0.2％
			Water		8.4％
IGF-1		0.50％
			Cholecalciferol		0.3％
Milk calcium		0.3％
			CPP		0.3％

Wherein, the prescription of emulsifing thickener is (weight portion): 1 part of molecule distillating monoglyceride, 0.4 part of polyglycerol ester, 0.1 part of carragheen, 0.2 part of xanthans.

Preparation process is identical with embodiment 1, and the result is as follows in the examination and test of products:

Project	Index
		Total solid	12.75％
Lactose	4.6％
		Protein	2.90％
Fat	3.55％

IGF-1	16.8mg/100g
		Vitamin D 3	151IU/100g
Calcium	150.0mg/100g
		Potassium	147.0mg/100g
Phosphorus	67.0mg/100g

Embodiment 7, contain the measure of merit of the liquid milk of IGF-1

The resulting liquid milk of embodiment 1-6 was fed 90 days to the rat filling continuously, observe of the influence of the resulting sample of embodiment 1-6 normal rat bone bone weight; By dual intensity X line borne densitometers, measure of the influence of the resulting sample of embodiment 1-6 to normal rat bone density, organize in contrast with common pasteurized milk, measure the bone mineral density after 90 days.

1 skeleton density regulating action

1.1 animal origin:

104 of this experimental selection cleaning level female sd inbred rats, body weight is the 180-210 gram, purchases the Experimental Animal Center in the Department Of Medicine, Peking University.It is 30-50% that experimental rat is raised in humidity, and temperature 20-25 ℃ of drylot feeding raised in cages under the condition, 4 in every cage, and ad lib standard base pellet is freely drunk water.

1.2 dosage design:

This sample human body RD is to restrain/60 kilograms every days 250, and basic, normal, high three dosage groups are not set up in this experiment, only adopt identical absorption dosage, and the blank group is irritated stomach with common sterilization cow's milk.

1.3 laboratory sample preparation:

Accurately measure 100 milliliters liquid milk, adding distil water is mixed with 200 milliliters of test liquids that are the dosage group.

1.4 giving and sample collection of experimental rat specimen:

With the test experiments liquid of having prepared, irritate stomach according to 20 milliliters/100 gram BW.Once a day, the continuous irrigation stomach is 93 days.Irritate stomach after 93 days, rat is handled in fasting 12 hours, gets rat bilateral femur and shin bone, picks clean attachment, places valve bag respectively, and numbering waits bone density to be determined.

1.5 main laboratory apparatus and equipment:

Dual intensity X line borne densitometers: EDXA U.S. Norland XR-36 type.

Rigorous analysis balance: MD100-2.

1.6 experiment observation index and method:

Femur density and BMC: adopt dual intensity X line borne densitometers.Measure femur distal end, femoral shaft stage casing and three positions of femur proximal part respectively, calculate femur density (g/cm ²) and BMC (g).

1.7 Data Processing in Experiment:

Carry out the statistical analysis of data with the SPSS10.0 statistical software, every testing index all adopts mean ± standard deviation to represent, adopt relatively significant difference between each group of one-way analysis of variance, relatively, select the critical value of P＜0.05 between organizing with LSD (least significant difference method) as the inspection statistics conspicuousness.

1.8 experimental result

Test liquid sees Table 1 to the influence of rat femur density;

Table 1 control group and test liquid are to rat femur density measurement result (g/cm ²)

(through variance analysis) compared with normal sterilizing flavouring breast control group in P＜0.05

By above data as seen, embodiment 6, embodiment 5 products all obviously are better than embodiment 4, embodiment 3, embodiment 2, embodiment 1, control group for the effect that increases rat bone mineral density; The experiment effect basically identical of embodiment 4, embodiment 3, embodiment 2, embodiment 1 but all significantly be better than control group wherein.

1.9 conclusion:

The result shows that the product of the IGF-1 of containing of the present invention has the effect that increases skeleton density.

Claims (3)

1, a kind of liquid milk, contain the component of following weight portion:

Milk 50-90;

IGF-1 0.25-0.50；

Emulsifing thickener 0.1-0.3;

Water 8.20-49.6.

2, liquid milk according to claim 1 is characterized in that, also contains the component of one or more following weight portions in the described liquid milk:

Vitamin V _D30.1-0.3;

CPP 0.1-0.3;

Milk calcium 0.1-0.3.

3, liquid milk according to claim 1 and 2 is characterized in that: described emulsifing thickener comprises molecule distillating monoglyceride, polyglycerol ester, xanthans and carragheen, and its weight fraction is such as following:

Molecule distillating monoglyceride 1,

Polyglycerol ester 0.2-0.4,

Carragheen 0.1-0.2,

Xanthans 0.2-0.4.