CN1885037A - Full-automatic enzyme-linked immunoassay system - Google Patents
Full-automatic enzyme-linked immunoassay system Download PDFInfo
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- CN1885037A CN1885037A CN 200510043875 CN200510043875A CN1885037A CN 1885037 A CN1885037 A CN 1885037A CN 200510043875 CN200510043875 CN 200510043875 CN 200510043875 A CN200510043875 A CN 200510043875A CN 1885037 A CN1885037 A CN 1885037A
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Abstract
The invention relates to a full-automatic enzyme couple immune analyze system, used in ELISA test of antigen and antibody of medical organizations, which can overcome the defects of low efficiency. The invention uses enzyme couple immune processing method of industrial product line, to distribute the structure based on needed program and condition of enzyme couple immune reaction, to realize the liquid adding unit, the constant-temperature cultivate unit and plate washing unit of said reaction, which can independently complete mission and cooperate too. The invention can improve the efficiency 1-10 times than present instruments.
Description
Technical field
The present invention relates to a kind of full-automatic enzyme-linked immunoassay instrument.In each medical institutions, the antigen, antibody etc. that are mainly used in each viroid such as hepatitis A to the patient, hepatitis B, acquired immune deficiency syndrome (AIDS) carry out the automated analysis instrument that euzymelinked immunosorbent assay (ELISA) detects.
Background technology
Enzyme-linked immunosorbent assay (ELISA method) is the new discipline that modern development gets up, and it is specificity and the catalysis amplification that utilizes enzyme, detects the classical way of various micro-antigens, antibody.Departments such as each large, medium and small hospital and blood station, epidemic prevention station, scientific research all mainly adopt this detection method to judge whether the patient has infectd certain virus or produced certain antibody at present.
Enzyme-linked immuno assay is as a basic processing unit with 96 empty ELISA Plate.Enzyme linked immunoassay is according to the difference of project, and desired reactions steps and condition are had nothing in common with each other.But generally all to pass through following several steps.1, adds sample, add reagent again; 2,37 degree constant temperature are hatched; 3, cleaning of enzyme target; 4, enzyme-added reagent; 5,37 degree constant temperature are hatched again; 6, wash plate again; 7, add developer; 8,37 degree are hatched once more; 9, add stop buffer; 10, interpretation as a result.Can see that in entire reaction course enzyme linked immunoassay requires: the one, in ELISA Plate, add 3 to 5 liquid; The 2nd, 37 degree constant temperature to hatch 2 to 3 times; The 3rd, cleaning of enzyme target 1 to 2 time.As seen the process of enzyme linked immunoassay is very complicated.
Present full-automatic enzyme-linked immunoassay instrument has all adopted a cover liquid adding device, a cover constant temperature hatches the unit and a cover is washed the plate unit.This design can't be carried out the test of next group enzyme linked immunological before instrument is all handled enzyme linked immunoassay on the last consignment of ELISA Plate, can only a collection of carrying out.This design just seems when treatment capacity is very big that especially efficient is very low.In the practice, the processing speed of these automatical analysis instruments is than going back slowly that several medical workers handle by hand.
Summary of the invention
In order to overcome the low difficult problem of this treatment effeciency of import full-automatic enzyme-linked immunoassay instrument, the present invention adopts industrial flow-line enzyme linked immunological disposal route, carries out the physical construction layout according to program and condition that enzyme linked immunoassay needs; The liquid adding device of enzyme linked immunoassay, constant temperature are hatched the unit and are washed the plate unit, can either independently finish the work separately under the unified control of computing machine, do not delay mutually, can unify cooperation again, finish course of reaction jointly.When the mass disposal enzyme was exempted from test event, the present invention can raise the efficiency 1 to 10 times with quasi-instrument than import.Measure greatly more, efficient improves obviously more.
The technical solution adopted for the present invention to solve the technical problems:
With three cover liquid adding devices, three covers hatch the unit, two covers are washed plate unit, cover interpretation units in series as a result together, arrange in the following order: liquid adding device → once hatch unit → once wash plate unit → secondary liquid adding device → secondary is hatched unit → secondary and is washed plate unit → three time liquid adding device → three and time hatch interpretation unit, unit → as a result.This has just constituted the full-automatic enzyme-linked immunoassay system of a pipeline system.
The key problem in technology of full-automatic enzyme-linked immunoassay system be exactly in the indoor design of instrument track, with one or more ELISA Plate be placed on one the carrying carrier on, will carry support card again and go into track inside.In the bottom of track, a master control servomotor is installed, servomotor is controlled the position of carrying carrier under the control of master control computer.The carrying carrier can be under the control of motor orbital motion.Track also can adopt conveyer structure.The carrying carrier can be reused.When the carrier movement that is carrying ELISA Plate during to liquid adding device place, the liquid adding device just comes into operation; Equally, hatch the unit, perhaps wash the plate unit, can both begin corresponding operation during the interpretation unit as a result when it moves to.This system leaves the liquid adding device one time at a last carrier, after entering constant temperature and hatching the unit, can add the carrier that another is carrying different ELISA Plate at once, carries out liquid and adds, thereby begun another group enzyme linked immunoassay process.By that analogy, whole process constantly goes on as streamline, adds new ELISA Plate continuously at starting end, constantly reads the result at the end end, so just can improve the efficient that enzyme exempts to test greatly.
Description of drawings
Below in conjunction with drawings and Examples the utility model is further specified.
Fig. 1 provides vertical part section structural representation of full-automatic enzyme-linked immunoassay system
Among the figure, 1, liquid adding device, 2, once hatch the unit, 3, once wash the plate unit, 4, secondary liquid adding device, 5, secondary hatches the unit, 6, secondary is washed the plate unit, and 7, three liquid adding devices are hatched the unit 8, three times, 9, interpretation unit as a result, 10, carrying carrier, 11, servomotor, 12, bearing track, 13, general control system, 14, useless plate case.
Embodiment
After the medical worker is ready to all ingredients and sample to be tested, ELISA Plate that will test item is placed on the carrying carrier, to carry carrier (10) then and snap in track (12), liquid adding device this moment (1) will be automatically be assigned to sample to be tested and reagent in the ELISA Plate hole on the carrier according to the requirement of prior setting; After finishing, carrier (10) moves to and once hatches unit (2) under the drive of servomotor (11), and the constant temperature that carries out 37 ℃ is hatched.Simultaneously, the support card that another can carried ELISA Plate is gone in the track, carries out application of sample again, indicates the beginning of next group reaction.After carrier (10) was hatched and finished, servomotor (11) was brought carrier (10) into and is once washed plate unit (3), carries out the cleaning of ELISA Plate; After cleaning was finished, servomotor (11) took carrier (10) to secondary liquid adding device (4) again, carried out the interpolation of another kind of or two or more reagent; After, carry out secondary successively again and hatch that unit (5), secondary are washed plate unit (6), three liquid adding devices (7), hatched unit (8) for three times, the operation of interpretation unit (9) as a result.After this process was all finished, servomotor (11) was taken carrier (10) out of track, abandoned useless plate case (14).
Carrier and ELISA Plate are being hatched or during application of sample, servomotor (11) can have time enough under the control of general control system, put down this carrier, controls the reactions steps of other carriers, guarantee uninterruptedly carrying out of test process.
Be fast reaction speed, save the reaction time, the present invention will utilize the electromagnetism wave energy to accelerate the principle of enzyme linked immunoassay speed, and electromagnetic wave generator is installed in hatching the unit.
The enzyme linked immunoassay process of embodiment ()---dual-antigen sandwich method for determining antibody of AIDS virus
Its reactions steps requires as follows: at first add 100 μ l samples in the micropore of the micro reaction plate (also being ELISA Plate) of HIV specific antigen bag quilt, hatched 60 minutes in 37 environment of spending then, wash plate 5 times with washing the plate machine; In micropore, add enzyme conjugates 100 μ l again, carry out that secondary was hatched 30 minutes and secondary is washed plate; Substrate buffer solution 50 μ l and colour developing liquid 50 μ l are added in the micropore, 37 degree constant temperature were hatched 10 minutes, added the stop buffer of 50 μ l more again, and course of reaction finishes.ELISA Plate is put into microplate reader carry out interpretation as a result.
In implementation process, put ELISA Plate into the carrying carrier, will carry support card again and go into track.Begin by the computer control course of reaction.A liquid adding device begins sample 100 μ l are added in the micropore of ELISA Plate.After finishing, the master control motor will carry carrier and be dragged to and once hatch the unit and carry out 37 degree and hatched 60 minutes, simultaneously second carrying support card can be gone into track, carry out the enzyme linked immunoassay of next group; After finishing, the master control motor will carry carrier and be dragged to and once wash the plate unit, carry out 5 washings; After finishing, the master control motor will carry carrier again and be dragged to secondary liquid adding device, and 100 μ l add in the micropore with enzyme conjugates, carry out secondary then and hatch 30 minutes and secondary and wash plate; Enter the liquid adding device again three times, substrate buffer solution 50 μ l and colour developing liquid 50 μ l are added in the micropore, and then carry out 10 minutes hatch, return the liquid adding device again three times, the stop buffer that adds 50 μ l more directly enters interpretation unit (microplate reader) as a result, carries out interpretation as a result.After finishing whole process, the carrying carrier is hauled out track together with ELISA Plate and is abandoned.
The enzyme linked immunoassay of embodiment (two)---hepatitis B five (hepatitis b virus s antigen, surface antibodies etc.)
Can use two cover liquid to add, hatch, wash the system that the plate unit constitutes.As mentioned above, different is not need to carry out the operation that secondary liquid adds, secondary is hatched, secondary is washed plate.
Claims (4)
1, a kind of full-automatic enzyme-linked immunoassay system, comprise that liquid adding device, constant temperature are hatched the unit, washed the plate unit, interpretation unit as a result, it is characterized in that: this system by the above liquid adding device of two covers or two covers, hatch the unit and wash the plate unit and constitute, bottom in each unit, inside of instrument is provided with track, mode with industrial flow-line, according to the requirement of enzyme linked immunoassay process, each sequence of unit is cascaded.
2, full-automatic enzyme-linked immunoassay system according to claim 1 is characterized in that: this system is provided with the carrying carrier that can snap in track, and carrier can carry one or more ELISA Plate.
3, full-automatic enzyme-linked immunoassay system according to claim 1 is characterized in that: constant temperature is hatched the unit and has been adopted that electromagnetic wave is auxiliary hatches.
4, full-automatic enzyme-linked immunoassay system according to claim 1 is characterized in that: the track of described carrying carrier also can adopt conveyer structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510043875 CN1885037A (en) | 2005-06-22 | 2005-06-22 | Full-automatic enzyme-linked immunoassay system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510043875 CN1885037A (en) | 2005-06-22 | 2005-06-22 | Full-automatic enzyme-linked immunoassay system |
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| CN1885037A true CN1885037A (en) | 2006-12-27 |
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| CN 200510043875 Pending CN1885037A (en) | 2005-06-22 | 2005-06-22 | Full-automatic enzyme-linked immunoassay system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102147405A (en) * | 2011-01-12 | 2011-08-10 | 深圳市亚辉龙生物科技有限公司 | Fully automatic biochemical immune analyzer |
| WO2013044454A1 (en) | 2011-09-27 | 2013-04-04 | 深圳市亚辉龙生物科技有限公司 | Full-automatic immunity analyzer and detection method thereof |
-
2005
- 2005-06-22 CN CN 200510043875 patent/CN1885037A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102147405A (en) * | 2011-01-12 | 2011-08-10 | 深圳市亚辉龙生物科技有限公司 | Fully automatic biochemical immune analyzer |
| CN102147405B (en) * | 2011-01-12 | 2013-08-21 | 深圳市亚辉龙生物科技有限公司 | Fully automatic biochemical immune analyzer |
| WO2013044454A1 (en) | 2011-09-27 | 2013-04-04 | 深圳市亚辉龙生物科技有限公司 | Full-automatic immunity analyzer and detection method thereof |
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Effective date of registration: 20080711 Address after: Overseas Students Pioneer Park, Yantai Development Zone, Shandong, 3#649 Applicant after: Yantai Addcare Bio-Tech Co., Ltd. Address before: No. 21 Li Nong Road, Lixia District, Ji'nan, Shandong Applicant before: Jinan Tianjikang Bioengineering Co., Ltd. |
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Open date: 20061227 |