CN1635139A - Method for fermenting and producing α-arbutin - Google Patents
Method for fermenting and producing α-arbutin Download PDFInfo
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- CN1635139A CN1635139A CN 200410090980 CN200410090980A CN1635139A CN 1635139 A CN1635139 A CN 1635139A CN 200410090980 CN200410090980 CN 200410090980 CN 200410090980 A CN200410090980 A CN 200410090980A CN 1635139 A CN1635139 A CN 1635139A
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- BJRNKVDFDLYUGJ-ZIQFBCGOSA-N alpha-Arbutin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC1=CC=C(O)C=C1 BJRNKVDFDLYUGJ-ZIQFBCGOSA-N 0.000 title claims abstract description 50
- 229940033280 alpha-arbutin Drugs 0.000 title claims abstract description 50
- 238000000034 method Methods 0.000 title claims abstract description 20
- 238000000855 fermentation Methods 0.000 claims abstract description 31
- 230000004151 fermentation Effects 0.000 claims abstract description 31
- 241000589636 Xanthomonas campestris Species 0.000 claims abstract description 21
- 238000006243 chemical reaction Methods 0.000 claims abstract description 17
- 238000004519 manufacturing process Methods 0.000 claims abstract description 7
- 239000007788 liquid Substances 0.000 claims description 41
- GHMLBKRAJCXXBS-UHFFFAOYSA-N resorcinol Chemical compound OC1=CC=CC(O)=C1 GHMLBKRAJCXXBS-UHFFFAOYSA-N 0.000 claims description 26
- 239000011347 resin Substances 0.000 claims description 24
- 229920005989 resin Polymers 0.000 claims description 24
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 20
- 229930006000 Sucrose Natural products 0.000 claims description 20
- 239000005720 sucrose Substances 0.000 claims description 20
- 230000001580 bacterial effect Effects 0.000 claims description 19
- 239000003463 adsorbent Substances 0.000 claims description 16
- 238000012545 processing Methods 0.000 claims description 15
- 239000001888 Peptone Substances 0.000 claims description 12
- 108010080698 Peptones Proteins 0.000 claims description 12
- 235000019319 peptone Nutrition 0.000 claims description 12
- 238000011534 incubation Methods 0.000 claims description 11
- 238000002156 mixing Methods 0.000 claims description 11
- 238000002360 preparation method Methods 0.000 claims description 11
- 238000000926 separation method Methods 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 239000000843 powder Substances 0.000 claims description 10
- 238000000746 purification Methods 0.000 claims description 10
- 238000011081 inoculation Methods 0.000 claims description 9
- 239000002054 inoculum Substances 0.000 claims description 8
- 239000000376 reactant Substances 0.000 claims description 8
- 238000012360 testing method Methods 0.000 claims description 7
- 238000001291 vacuum drying Methods 0.000 claims description 7
- 229920001817 Agar Polymers 0.000 claims description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 6
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 6
- 230000000274 adsorptive effect Effects 0.000 claims description 6
- 239000008272 agar Substances 0.000 claims description 6
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical class [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 6
- 235000012204 lemonade/lime carbonate Nutrition 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 5
- 230000035484 reaction time Effects 0.000 claims description 5
- 238000009423 ventilation Methods 0.000 claims description 5
- 239000003960 organic solvent Substances 0.000 abstract description 2
- QIGBRXMKCJKVMJ-UHFFFAOYSA-N Hydroquinone Chemical compound OC1=CC=C(O)C=C1 QIGBRXMKCJKVMJ-UHFFFAOYSA-N 0.000 abstract 2
- 102000003425 Tyrosinase Human genes 0.000 abstract 2
- 108060008724 Tyrosinase Proteins 0.000 abstract 2
- 206010014970 Ephelides Diseases 0.000 abstract 1
- 208000003351 Melanosis Diseases 0.000 abstract 1
- 239000007844 bleaching agent Substances 0.000 abstract 1
- 230000008021 deposition Effects 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 239000002609 medium Substances 0.000 description 9
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical class CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 description 6
- 229940066779 peptones Drugs 0.000 description 6
- 230000009466 transformation Effects 0.000 description 4
- 231100000219 mutagenic Toxicity 0.000 description 3
- 230000003505 mutagenic effect Effects 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- KIWBPDUYBMNFTB-UHFFFAOYSA-N Ethyl hydrogen sulfate Chemical compound CCOS(O)(=O)=O KIWBPDUYBMNFTB-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000012262 fermentative production Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000003716 rejuvenation Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a method for preparing alpha-arbutin which comprises steps of preparing slope seeds, selecting culture mediums, optimizing reaction conditions and extracting products and so on. The invention employs Xanthomonas campestris to ferment to produce the alpha-arbutin without use of organic solvents during the production and generation of harmful substances during the fermentation process. The invention has advantages of low production cost, simple process, and safe operation. And the alpha-arbutin yield produced by the method is high, the alpha-arbutin content in fermentation liquor is 8-11g/L, and the conversion rate of the hydroquinone can be 91%. The alpha-arbutin can obviously inhibit activities of tyrosinase, reduce tyrosinase deposition in skins, and bleach skins, remove freckles.
Description
Technical field
The present invention relates to the preparation method of alpha-arbutin.
Background technology
At present, domestic document for the synthetic alpha-arbutin of biological process does not appear in the newspapers as yet; The preparation method of external alpha-arbutin mainly is by being catalyzer with the α Glycosylase, synthetic Resorcinol of catalysis and glucose are alpha-arbutin, because the α Glycosylase costs an arm and a leg, so the alpha-arbutin cost of producing is very high, and adopt method for preparing alpha-arbutin productive rate not high, brought very big difficulty to actual applying.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of alpha-arbutin, this preparation method is by the bacterial strain xanthomonas campestris CGMCC NO.1243 that xanthomonas campestris is screened, very high, the anti-Resorcinol ability of a mutagenic obtained strain alpha-arbutin productive rate is strong, select for use suitable medium under optimal culture conditions, to cultivate certain hour, add reactant Resorcinol and sucrose, produce alpha-arbutin at the optimum reaction condition bottom fermentation, behind the separation of fermented liquid by macroporous adsorptive resins, the purifying, can obtain highly purified alpha-arbutin.This method has advantages such as technological operation is simple, production cost is low, operational safety.
Overall technology design of the present invention is:
The fermentative Production alpha-arbutin, this method is made up of following process steps:
(1) with the slant medium bevel bacterial classification of xanthomonas campestris (Xanthomonas campestris) CGMCC NO.1243 bacterial classification inoculation in the test tube;
(2) with inclined-plane bacterial strain enlarged culturing as seed liquor;
(3) seed liquor is inserted in the fermentor tank ferment;
(4) add reactant Resorcinol and sucrose;
(5) separation and purification of alpha-arbutin.
Concrete processing step of the present invention and processing condition are:
Slant medium is made up of following parts by weight of component in the step (1):
1000 parts in sucrose 40-60 part, peptone 10-20 part, yeast powder 2.0-3.5 part, 0.5 part in sal epsom, 2.0 parts of dipotassium hydrogen phosphates, 2.0 parts in sodium-chlor, agar 15-20 part and water;
The processing condition of preparation slant strains are that the pH value is that 7.0-8.0, temperature 25-35 ℃, incubation time are 24-48 hour.
The middle fermented liquid of seed liquor and step (3) is made up of following parts by weight of component in the step (2):
1000 parts in sucrose 40-60 part, peptone 10-20 part, sal epsom 0.5-1.0 part, 2 parts in lime carbonate and water;
The processing condition of fermentation are 10-15% for the fermented liquid inoculum size, and the pH value is 7.0-7.5, and culture temperature is 25-35 ℃, and ventilation is 0.4-0.6vvm, and mixing speed is 120-160 rev/min, and fermentation time is 24-36 hour.Reaction conditions is in the step (4): reactant Resorcinol amount concentration is 30-48mmol/l, and sucrose amount concentration is 100-200mmol/l, and temperature of reaction is 30-55 ℃, and the reaction times is 36-72 hour, and mixing speed is 160-200 rev/min.Under this reaction conditions, the transformation efficiency that Resorcinol is converted into alpha-arbutin reaches 87-92%, contains alpha-arbutin 8-11g in every liter of fermented liquid.
The separation and purification of alpha-arbutin comprises following four steps in the step (5);
A, the fermented liquid after fermentation finished under 4000 rev/mins of conditions centrifugal 20 minutes;
B, decolouring by the low-pole macroporous adsorptive resins through the fermented liquid after a step, the low-pole macroporous adsorptive resins is a macroporous adsorbent resin AB-8 post, through the fermented liquid after a step through the absorption of AB-8 post after, fermented liquid becomes colourless transparent liquid;
C, the fermented liquid through the b step is carried out separation and purification by polar macroporous adsorbent resin column, polar macroporous adsorbent resin column is a macroporous adsorbent resin S-8 post;
D, fermented liquid vacuum-drying through the c step, can obtain purity and be the alpha-arbutin 98% or more, vacuum-drying vacuum tightness is 0.1Mpa, temperature is 65 to spend, dried finished products is a white powder.
Used xanthomonas campestris (Xanthomonas campestris) the CGMCC NO.1243 of the present invention is in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, and preserving number is xanthomonas campestris CGMCCNO.1243.
The screening of xanthomonas campestris CGMCC NO.1243, form by following process steps:
(1) ampere is managed xanthomonas campestris bacterial classification inoculation inclined-plane and make slant strains;
(2) with bacterial classification spread plate after the rejuvenation;
(3) flat-plate bacterial colony is inoculated shake flask fermentation and cultivated, carry out primary dcreening operation and multiple sieve;
(4) bacterial strain after will sieving again carries out complex mutation;
Plate culture medium in the step (1) in slant medium and the step (2) is made up of following parts by weight of component:
1000 parts in sucrose 40-60 part, peptone 10-20 part, yeast powder 2.0-3.5 part, 0.5 part in sal epsom, 2.0 parts of dipotassium hydrogen phosphates, 2.0 parts in sodium-chlor, agar 15-20 part and water;
Preparation slant strains and dull and stereotyped processing condition are that the pH value is that 7.0-8.0, temperature 25-35 ℃, incubation time are 24-48 hour.
Fermented liquid is made up of following parts by weight of component in the step (3):
1000 parts in sucrose 40-60 part, peptone 10-20 part, sal epsom 0.5-1.0 part, 2 parts in lime carbonate and water;
The processing condition of fermentation are: the pH value is 7.0-7.5, and culture temperature is 25-35 ℃, and mixing speed is 120-160 rev/min, and fermentation time is 24-36 hour.
Mutagenesis comprises the preparation of seed bacteria suspension, the add-on of mutagenic compound, time of ultraviolet irradiation successively in the step (4).
Slant strains is inoculated into contains in the 5ml physiological saline test tube, adding volume percent in the test tube is the ethyl sulfate mutagenic compound of 0.5%-2.0%, 1-2min vibrates on the vortex oscillation device, get this bacteria suspension spread plate of 0.5ml, flat board is placed on ultra violet lamp 20-60s, then flat board is secretly cultivated.Culture condition is: culture temperature 25-35 ℃, incubation time are 48-72 hour.
At last, obtain xanthomonas campestris (Xanthomonas campestris) CGMCC NO.1243.
The obtained technical progress of the present invention is:
The present invention is by xanthomonas campestris CGMCC NO.1243 preparing alpha-arbutin through fermentation, has reduced production cost, and in the production process not with an organic solvent, do not produce objectionable impurities in the fermenting process, processing step is simple, has improved the production operation security.The alpha-arbutin output height that adopts the present invention to produce contains the 8-11g alpha-arbutin in every liter of fermented liquid, by the macroporous adsorbent resin separation and purification, purity can reach more than 98%, has great industrialization meaning.
Embodiment: the present invention is described further below in conjunction with embodiment.
Embodiment 1
(1) strain inclined plane is cultivated
With the slant medium bevel bacterial classification of xanthomonas campestris CGMCC NO.1243 bacterial classification inoculation in the test tube;
Wherein the strain inclined plane substratum is made up of following parts by weight of component:
1000 parts in 55 parts of sucrose, 15 parts of peptones, 3 parts of yeast powders, 0.5 part in sal epsom, 2.0 parts of dipotassium hydrogen phosphates, 2.0 parts in sodium-chlor, 15 parts in agar and water
The processing condition of preparation slant strains are that the pH value is 7.0,30 ℃ of temperature, incubation time are 36 hours.
(2) inoculation fermentation
With inclined-plane bacterial strain enlarged culturing as seed liquor;
Seed liquor inserted in the fermentor tank by 10% inoculum size ferment;
Wherein seed liquor and fermented liquid are made up of following parts by weight of component:
1000 parts in 55 parts of sucrose, 15 parts of peptones, 1.0 parts in sal epsom, 2 parts in lime carbonate and water
The processing condition of fermentation are 10% for the fermented liquid inoculum size, and the pH value is 7.0, and culture temperature is 30 ℃, and ventilation is 0.6vvm, and mixing speed is 130 rev/mins, and incubation time is 36 hours.
(3) produce alpha-arbutin
Adding reactant Resorcinol amount concentration is that 35mmol/l and sucrose amount concentration are 150mmol/l;
Wherein reaction conditions is: temperature of reaction is 35 ℃, and the reaction times is 48 hours, and mixing speed is 200 rev/mins.Under this reaction conditions, the transformation efficiency that Resorcinol is converted into alpha-arbutin reaches 91.5%, contains alpha-arbutin 8.5g in every liter of fermented liquid.
(4) separation and purification of alpha-arbutin comprises following four steps:
A, the fermented liquid after fermentation finished under 4000 rev/mins of conditions centrifugal 20 minutes
Low-pole macroporous adsorptive resins in b, the step is a macroporous adsorbent resin AB-8 post, and after adsorbing through the AB-8 post through the fermented liquid after a step, fermented liquid becomes colourless transparent liquid.
Polar macroporous adsorbent resin column in c, the step is a macroporous adsorbent resin S-8 post.
Vacuum-drying vacuum tightness in d, the step is 0.1Mpa, and temperature is 65 ℃, and dried finished products is a white powder, and purity reaches 99%.
Embodiment 2
(1) strain inclined plane is cultivated
With the slant medium bevel bacterial classification of xanthomonas campestris CGMCC NO.1243 bacterial classification inoculation in the test tube;
Wherein slant medium is made up of following parts by weight of component:
1000 parts in 45 parts of sucrose, 10 parts of peptones, 2.5 parts of yeast powders, 0.5 part in sal epsom, 2.0 parts of dipotassium hydrogen phosphates, 2.0 parts in sodium-chlor, 15 parts in agar and water
The processing condition of preparation slant strains are that the pH value is 7.5,30 ℃ of temperature, incubation time are 24 hours.
(2) inoculation fermentation
With inclined-plane bacterial strain enlarged culturing as seed liquor;
Seed liquor inserted in the fermentor tank by 15% inoculum size ferment;
Wherein seed liquor and fermented liquid are made up of following parts by weight of component:
1000 parts in 45 parts of sucrose, 10 parts of peptones, 1.0 parts in sal epsom, 2 parts in lime carbonate and water
The processing condition of fermentation are 15% for the fermented liquid inoculum size, and the pH value is 7.5, and culture temperature is 30 ℃, and ventilation is 0.5vvm, and mixing speed is 140 rev/mins, and incubation time is 24 hours.
(3) produce alpha-arbutin
Adding reactant Resorcinol amount concentration is that 40mmol/l and sucrose amount concentration are 200mmol/l;
Wherein reaction conditions is: temperature of reaction is 40 ℃, and the reaction times is 48 hours, and mixing speed is 200 rev/mins.Under this reaction conditions, the transformation efficiency that Resorcinol is converted into alpha-arbutin reaches 90.2%, contains alpha-arbutin 9.6g in every liter of fermented liquid.
(4) separation and purification of alpha-arbutin comprises following four steps:
A, the fermented liquid after fermentation finished under 4000 rev/mins of conditions centrifugal 20 minutes;
Low-pole macroporous adsorbent resin in b, the step is a macroporous adsorbent resin AB-8 post, and after adsorbing through the AB-8 post through the fermented liquid after a step, fermented liquid becomes colourless transparent liquid.
Polar macroporous adsorption resin in c, the step is a macroporous adsorbent resin S-8 post.
Vacuum-drying vacuum tightness in d, the step is 0.1Mpa, and temperature is 65 ℃, and dried finished products is a white powder, and purity reaches 98.3%.
Embodiment 3
(1) strain inclined plane is cultivated
With the slant medium bevel bacterial classification of xanthomonas campestris CGMCC NO.1243 bacterial classification inoculation in the test tube;
Wherein slant medium is made up of following parts by weight of component:
1000 parts in 55 parts of sucrose, 10 parts of peptones, 2.0 parts of yeast powders, 0.5 part in sal epsom, 2.0 parts of dipotassium hydrogen phosphates, 2.0 parts in sodium-chlor, 15 parts in agar and water
The processing condition of preparation slant strains are that the pH value is 7.5,30 ℃ of temperature, incubation time are 36 hours.
(2) inoculation fermentation
With inclined-plane bacterial strain enlarged culturing as seed liquor;
Seed liquor inserted in the fermentor tank by 12.5% inoculum size ferment;
Wherein seed liquor and fermented liquid are made up of following parts by weight of component:
55 parts of sucrose, 10 parts of peptones, 1.0 parts in sal epsom, 2 parts and 1000 parts in lime carbonate
The processing condition of fermentation are 12.5% for the fermented liquid inoculum size, and the pH value is 7.5, and culture temperature is 30 ℃, and ventilation is 0.4vvm, and mixing speed is 160 rev/mins, and incubation time is 36 hours.
(3) produce alpha-arbutin
Adding reactant Resorcinol amount concentration is that 45mmol/l and sucrose amount concentration are 200mmol/l;
Reaction conditions is: temperature of reaction is 50 ℃, and the reaction times is 48 hours, and mixing speed is 200 rev/mins.Under this reaction conditions, the transformation efficiency that Resorcinol is converted into alpha-arbutin reaches 87.0%, contains alpha-arbutin 10g in every liter of fermented liquid.
(4) separation and purification of alpha-arbutin comprises following four steps:
A, the fermented liquid after fermentation finished under 4000 rev/mins of conditions centrifugal 20 minutes;
Low-pole macroporous adsorbent resin in b, the step is a macroporous adsorbent resin AB-8 post, and after adsorbing through the AB-8 post through the fermented liquid after a step, fermented liquid becomes colourless transparent liquid.
Polar macroporous adsorption resin in c, the step is a macroporous adsorbent resin S-8 post.
Vacuum-drying vacuum tightness in d, the step is 0.1Mpa, and temperature is 65 ℃, and dried finished products is a white powder, and purity reaches 98.0%.
Claims (9)
1, the method for preparing alpha-arbutin through fermentation is characterized in that: use the xanthomonas campestris preparing alpha-arbutin through fermentation, production process is made up of following process steps:
(1) with the slant medium bevel bacterial classification of xanthomonas campestris (Xanthomonas campestris) CGMCC NO.1243 bacterial classification inoculation in the test tube;
(2) with the slant strains enlarged culturing as seed liquor;
(3) seed liquor is inserted in the fermentor tank ferment;
(4) add reactant Resorcinol and sucrose;
(5) separation and purification of alpha-arbutin.
2, the method for preparing alpha-arbutin through fermentation according to claim 1 is characterized in that: the slant medium in described (1) step is made up of following parts by weight of component:
1000 parts in sucrose 40-60 part, peptone 10-20 part, yeast powder 2.0-3.5 part, 0.5 part in sal epsom, 2.0 parts of dipotassium hydrogen phosphates, 2.0 parts in sodium-chlor, agar 15-20 part and water.
3, the method for preparing alpha-arbutin through fermentation according to claim 1 and 2 is characterized in that: the processing condition of preparation slant strains are: pH value is that 7.0-8.0, temperature 25-35 ℃, incubation time are 24-48 hour.
4, the method for preparing alpha-arbutin through fermentation according to claim 1 is characterized in that: fermented liquid is made up of following parts by weight of component in the seed liquor in described (2) step and (3) step:
1000 parts in sucrose 40-60 part, peptone 10-20 part, sal epsom 0.5-1.0 part, 2 parts in lime carbonate and water.
5, according to the method for claim 1 or 4 described preparing alpha-arbutin through fermentation, it is characterized in that: the processing condition of fermentation are: the fermented liquid inoculum size is 10-15%, the pH value is 7.0-7.5, culture temperature is 25-35 ℃, ventilation is 0.4-0.6vvm, mixing speed is 120-160 rev/min, and incubation time is 24-36 hour.
6, the method for preparing alpha-arbutin through fermentation according to claim 1, it is characterized in that: the reaction conditions in described (4) step is: reactant Resorcinol amount concentration is 30-48mmol/l, sucrose amount concentration is 100-200mmol/l, temperature of reaction is 30-55 ℃, reaction times is 36-72 hour, and mixing speed is 160-200 rev/min.
7, the method for preparing alpha-arbutin through fermentation according to claim 1 is characterized in that: the separation and purification of the alpha-arbutin in described (5) step comprises following four steps:
A, the fermented liquid after fermentation finished under 4000 rev/mins of conditions centrifugal 20 minutes;
B, decolouring by the low-pole macroporous adsorptive resins through the fermented liquid after a step;
C, the fermented liquid through the b step is carried out separation and purification by polar macroporous adsorbent resin column;
D, fermented liquid vacuum-drying through the c step, can obtain purity and be the alpha-arbutin more than 98%.
8, the method for preparing alpha-arbutin through fermentation according to claim 7 is characterized in that: described a step fermented liquid under 4000 rev/mins of conditions centrifugal 20 minutes; Low-pole macroporous adsorptive resins in the b step is the AB-8 post; Polar macroporous adsorbent resin column in the c step is the S-8 post; Vacuum-drying vacuum tightness in the d step is 0.1Mpa, and temperature is 65 degree, and dried finished products purity reaches more than 98%.
9. xanthomonas campestris (Xanthomonas campestris) CGMCC NO.1243.
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| CN 200410090980 CN1279163C (en) | 2004-11-15 | 2004-11-15 | Method for fermenting and producing α-arbutin |
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| CN 200410090980 CN1279163C (en) | 2004-11-15 | 2004-11-15 | Method for fermenting and producing α-arbutin |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100372861C (en) * | 2005-07-21 | 2008-03-05 | 华东理工大学 | A kind of preparation method of α-arbutin |
| CN101701237B (en) * | 2009-11-05 | 2011-08-31 | 北京化工大学 | Method for producing alpha-glucosyl eugenol by fermentation |
| CN102978265A (en) * | 2012-12-03 | 2013-03-20 | 韦慧芳 | Method for synthesizing alpha-arbutin by enzymic method through catalysis |
| CN104498565A (en) * | 2014-09-11 | 2015-04-08 | 北京化工大学 | Method for co-production of alpha-arbutin and xanthan gum by xanthomonas |
| CN104761593A (en) * | 2015-04-15 | 2015-07-08 | 南昌大学 | Method for extracting a-arbutin from fermentation liquor based on membrane separation technology |
| CN103483401B (en) * | 2013-09-24 | 2016-08-10 | 南京工业大学 | Method for separating hydroquinone from alpha-arbutin fermentation liquor |
| CN106317136A (en) * | 2015-06-19 | 2017-01-11 | 中国科学院微生物研究所 | Method for separating alpha-arbutin from alpha-arbutin broth |
| CN107446844A (en) * | 2017-08-03 | 2017-12-08 | 浙江工业大学 | A kind of xanthomonas campestris and its application |
| CN107574201A (en) * | 2017-09-27 | 2018-01-12 | 江西诚志生物工程有限公司 | Using the method for fermentation method production β thymidines |
| CN108220355A (en) * | 2017-12-29 | 2018-06-29 | 安徽华恒生物科技股份有限公司 | The processing method of Production by Enzymes alpha-arbutin waste liquid and its application |
| CN115894580A (en) * | 2023-02-10 | 2023-04-04 | 广州市乾相生物科技有限公司 | Separation and purification method for whole-cell catalytic production of alpha-arbutin |
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2004
- 2004-11-15 CN CN 200410090980 patent/CN1279163C/en not_active Expired - Fee Related
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100372861C (en) * | 2005-07-21 | 2008-03-05 | 华东理工大学 | A kind of preparation method of α-arbutin |
| CN101701237B (en) * | 2009-11-05 | 2011-08-31 | 北京化工大学 | Method for producing alpha-glucosyl eugenol by fermentation |
| CN102978265A (en) * | 2012-12-03 | 2013-03-20 | 韦慧芳 | Method for synthesizing alpha-arbutin by enzymic method through catalysis |
| CN103483401B (en) * | 2013-09-24 | 2016-08-10 | 南京工业大学 | Method for separating hydroquinone from alpha-arbutin fermentation liquor |
| CN104498565A (en) * | 2014-09-11 | 2015-04-08 | 北京化工大学 | Method for co-production of alpha-arbutin and xanthan gum by xanthomonas |
| CN104761593B (en) * | 2015-04-15 | 2019-06-14 | 南昌大学 | A method for extracting α-arbutin from fermentation broth based on membrane separation technology |
| CN104761593A (en) * | 2015-04-15 | 2015-07-08 | 南昌大学 | Method for extracting a-arbutin from fermentation liquor based on membrane separation technology |
| CN106317136A (en) * | 2015-06-19 | 2017-01-11 | 中国科学院微生物研究所 | Method for separating alpha-arbutin from alpha-arbutin broth |
| CN106317136B (en) * | 2015-06-19 | 2018-12-25 | 中国科学院微生物研究所 | A method of separating ɑ-arbutin from ɑ-arbutin fermentation liquid |
| CN107446844A (en) * | 2017-08-03 | 2017-12-08 | 浙江工业大学 | A kind of xanthomonas campestris and its application |
| CN107574201A (en) * | 2017-09-27 | 2018-01-12 | 江西诚志生物工程有限公司 | Using the method for fermentation method production β thymidines |
| CN108220355A (en) * | 2017-12-29 | 2018-06-29 | 安徽华恒生物科技股份有限公司 | The processing method of Production by Enzymes alpha-arbutin waste liquid and its application |
| CN115894580A (en) * | 2023-02-10 | 2023-04-04 | 广州市乾相生物科技有限公司 | Separation and purification method for whole-cell catalytic production of alpha-arbutin |
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