CN1605346A - Vacuum-electrolysis and flocculation reclaiming technology for preparing - Google Patents
Vacuum-electrolysis and flocculation reclaiming technology for preparing Download PDFInfo
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Abstract
The present invention is the technological process of preparing 'functional peptide-Fe' with animal blood in biological engineering and technology. The preparation process includes blood sampling with vacuum cutter, dynamic anticoagulant treatment, immunological probe detection, collection of safe and health animal blood as material; centrifugal separation of red blood cell, haemolytic treatment; electrolysis of hematoglobin in vacuum electrolytic bath to crack hematoglobin into small molecular peptide including 'functional peptide-Fe' with molecular weight of 1-2 KDa and containing porphyrin Fe; and adding flocculating agent to flocculate and recover 'functional peptide-Fe' selectively. The said matter may be well absorbed and has the functions of replenishing iron, treating anemia, resisting oxidation, eliminating free radical, preventing cardiac and cerebral vascular diseases, etc. and no toxic side effect
Description
One, technical field
The invention belongs to animal blood high value added utilization technology, bioactive substance separation and Extraction technology of preparing, and have technical field of bioengineering in the physiologically active medical substance preparation process.Be particularly related to and adopt novel ionization electrolysis to combine with the vacuum degradation technique, separate the novel technique of purifying and combining, utilize the garbage blood preparation of animal to have the production method of " functional peptide-Fe " high activity, high-absorbable, high health-care function with specificity flocculation recovery technology.
Two, technical background
China is an animal husbandry big country, and the total amount of livestock for sale of domestic animal has ranked world's first, meanwhile, along with the increase of butchering quantity, has large quantities of by-product blood to produce.According to the statistical calculation of cutting apart of butchering trunk of main animal such as pig, cattle, sheep, by-product blood accounts for 5% of the weight of animals; That is to say that the living animal of whenever butchering (only) 100 kilograms just has 5 kilograms blood generation, therefore, China is annual to produce nearly 5,000,000,000 kilograms blood, and this is a huge biological available resources with high added value.And angle from medicinal of modern times, threpsology and health science, nutritive value and economic worth as animal byproducts blood are higher than the intra-class correlation goods far away, the comprehensive process of so huge living resources, the high value added utilization with medical value are imperative, and, the comprehensive exploitation that with blood is characteristic livestock products and high value added product bring bigger economic benefit also will for aquaculture and peasant with utilizing, and promotes the sustainable development of China's animal husbandry to greatest extent.But, still lack the high added value health care to blood, the research and the advanced process technology and the means of medicinal function at present.The subject matter of prior art is:
(1) butchering of China animal is to take to cut off carotid artery mostly at present, or stabs the direct depletion method of heart, allows blood directly flow to traditional mode of butchering in blood pond.Its drawback has: blood directly and the external world contacts, on the animal health or the intravital material of body can fall in the blood and directly pollute, be unfavorable for the comprehensive utilization of blood; A large amount of blood can run off with waste liquid, not only causes the waste of blood but also cause environmental pollution.At present from the sanitary legislation of American-European and japanese food and medicine, when animal blood is used as food and pharmacy, must use vacuum cutter to take a blood sample, prevent the repeated pollution of blood;
(2) to be that animal product carries out deep processing prerequisite in the detection of blood safety.But China had not also met the Active immunity detection of blood in slaughtering process at present, made and existed a big hidden danger in the animal blood product utilization;
(3) the utilization overwhelming majority of China's blood is to produce animal feed whole blood powder by drying, and taste is stench, digestibility is low, added value is low, and can only be used for feedstuff, and the high added value biological active substances in the blood component also fails to obtain exploitation;
(4) digesting and assimilating of blood products is still a big problem: Fibrinogen forms colloid after changing into fibrin under the effect of various activation factors, and and platelet, leukocyte, erythrocyte etc. form the not cohesion piece of capacitive, be difficult in by people or other animal digestion and absorb.Therefore, blood effective anticoagulant of reclaiming blood in the technology be separating of blood component the important step that blood carries out deep processing;
(5), the application of Related product is also arranged both at home and abroad, but mainly be to utilize whole hemoglobin about the utilization of hemoglobin as medical product.As iron supplement and treatment iron deficiency anemia, utilize the separation hemoglobin on the medical value of processing technique and product, to have many deficiencies: the low drug effect that influences of iron ion content, hemoglobin can not heat treated (heating can be solidified) sterilization be brought difficulty, eat the back and meet the gastric acid precipitation, thereby influence is digested and assimilated or the like;
(6) about the deep processing of chemorphines-iron product, development and application is arranged also abroad, its main method is to utilize the protease hydrolysis method aminoacid hydrolysis that hemoglobin is unnecessary is fallen, and obtains the higher ferrous porphyrin of iron content.But the shortcoming of this method is that protease hydrolysis cost height causes secondary pollution easily in the hydrolytic process, contact oxygen continually and can make ferrous ion be oxidized to ferric ion easily, loses biological activity;
(7) recovery of peptide-ferrum of generating after with the hemoglobin hydrolysis of protease mainly is to use molecular sieving, electronic disengaging or dialysis to break away from methods such as son, and efficient is low, purity is low, it is former to waste water, quantity of wastewater effluent is big.
Three, summary of the invention
(1) summary of the invention outline:
At the blood deep processing, particularly in medicinal and the deep processing of chemorphines-ferrum of food additive usefulness; exist such as above-mentioned 7 situations; the invention solves the protection of ferrous ion in the secondary pollution, the course of processing of blood in animal blood-letting (blood sampling) technology, the stability of " functional peptide-Fe " composition, and problem such as medical value exploitation.By, safety efficient, low cost, blood " functional peptide-Fe " processing technique of high added value and the exploitation of technology to a whole set of, provide a cover to combine with the ionization electrolysis with vacuum, separation and purification reclaims " functional peptide-Fe " production technology and the method that combines with flocculation, has improved the reasonable utilization rate of blood widely.Develop the medical value and the product of blood " functional peptide-Fe " simultaneously.
(2) technology implementation scheme of the present invention and process route:
" functional peptide-Fe " product is the fresh blood of animal to be extracted back-process vacuum ionization electrolysis-technical matters processing and refinings such as flocculation recovery by erythrocyte separation-hemoglobin form.Main preparing technique process is: the vacuum cutter blood sampling of (1) animal, gather the blood of qualified health; (2) the dynamic anticoagulant of blood; (3) Active immunity of blood eqpidemic disease detects, and detects and reject the blood that disease is arranged; (4) separation of hemoglobin, extraction, refining; (5) degradation technique of " functional peptide-Fe "; (6) the flocculation recovery technology of " functional peptide-Fe "; (7) technology of preparing of " functional peptide-Fe " end product etc.Its processing technology routine such as Fig. 4.
Four, the concrete implementation step of the present invention:
(1) pretreatment technology of animal blood
1. the totally-enclosed blood sampling system of vacuum cutter: this system is made up of four part institutes, and promptly 1) the double-layer hollow blood sampling knife; 2) vacuum tube; 3) crawling type vacuum pump; 4) blood reservoir.This system be by the heart of stabbing animal or aorta through the closed pipeline negative pressure extracting to the blood holding vessel, the foundation of this system solved fully animal in butchering the blood-letting process blood because kiss the earth or the booty that contacts animal body or animal body fluid or body surface are sneaked in the blood blood repeated pollution that causes.For the preparation of biological medicine, the exploitation of health food are laid a good foundation;
2. the Active immunity probe detection system of blood: the blood of gathering in the animal body of absolute no eqpidemic disease could be as of the present invention blood raw material, and the disease surveillance system of safety is the basic guarantee of preparation qualified products.Therefore, utilize most advanced world immune antibody detection method among the present invention, developed the immunological probe Automatic Measurement Technique of disease.Utilize the antigen preparation antibody and the antibody probe of known Animal diseases, be placed in the blood reservior of vacuum cutter blood sampling system, the vacuum cutter hemostix is at the fixing head number or empty when reaching a certain amount of blood once, if find have the blood of disease discarded at once (method of handling together by national regulation is disposed), and blood reservior carried out disinfection, to ensure the safety of the blood of being gathered;
3. the dynamic anticoagulant technology of blood: carry out anticoagulant and handle if the blood after the blood-letting is untimely, interactions such as the Fibrinogen in the blood, thrombin are solidified hemocyte etc., become insoluble sludged blood, thereby make the blood loss processability.The present invention has developed dynamic anticoagulant technology in conjunction with the vacuum cutter blood sampling system, i.e. 20 centimeters above the vacuum point of a knife, by an aperture anticoagulant that configures is connected vacuum cutter blood drawing hole by pipeline, and can be according to the consumption (anticoagulant adopts the compound anticoagulant of sodium citrate salt) of the size adjustment anticoagulant of blood flow, and can play complete anticoagulant effect with the mix homogeneously in the blood reservior that is flowing in of blood;
4. the technology of preparing of erythrocytic extraction and hemoglobin: separate by continuous centrifuge through the blood after the anticoagulant, or through after the natural sedimentation erythrocyte is separated with serum by knockout tower.The former separates soon, treating capacity is big, persistence good, but causes the hemocyte haemolysis that breaks when centrifugal treating easily; In latter's disjoint set, separating effect is also relatively good, but treating capacity is less, is suitable for small processing.Erythrocyte after the separation carries out haemolysis, preparation hemoglobin, and concrete grammar has: ultrasonic cell-break method, the Hyposmolality erythrocyte method of rising brokenly, cell membrane chemolysis haemolysis method etc.Carrying out centrifugalize behind the haemolysis removes cell membrane and obtains hemoglobin.
(2) degraded of the vacuum of hemoglobin and ionization electrolysis preparation " functional peptide-Fe "
1. the ionization electrolysis tech of hemoglobin: under the condition that NaCl or KCl or electrolyte that the two is formed with the mixture of any ratio exist, the ionization of switching on, near anode, can accumulate a large amount of electronics and form the reproducibility environment, increase reducing power enhancing along with electron concentration, when electronics reaches certain concentration, will play splitting action to the peptide bond in the hemoglobin, this is exactly a know-why of the present invention, utilize this know-why, can substitute proteolytic enzyme protolysate matter, obtain the needed peptide-Fe that contains ferrous porphyrin, i.e. " functional peptide-Fe ".The mechanism of its effect such as Fig. 1.
2. the indirect degradation technique of hemoglobin: also can in the 1st, electrolyte be carried out electrolytic treatments in advance in the present invention, with set electrolyte (electrolyte among Fig. 1 in the anode electrolytic tank) hemoglobin of degrading, can obtain inventing resulting effect in 1 equally.
3. the application of vacuum degradation technique in " functional peptide-Fe " production technology: can change the conformation of hemoglobin under the vacuum condition, the promotion electronics contacts with protein peptide chain, accelerates degradation reaction; Simultaneously, vacuum condition can completely cut off particularly contacting of oxygen of hemoglobin and air, avoids in " functional peptide-Fe " ferrous ion oxidized, generates ferric ion and makes " functional peptide-Fe " lose biological activity, and utilization is difficult for being absorbed by the body.
4. the resist technology of ferrous ion in " functional peptide-Fe ": ferrous ion is easy to oxidized generation ferric ion; in order to solve the problem of oxidation of ferrous ion; the vacuum condition that in the present invention, adopts, when separating the state of vacuum (being non-vacuum condition), invented and to have blocked ferrous ion (Fe in " functional peptide-Fe "
2+) (OH), its action principle is to utilize (Fe for the sense group hydroxyl that contacts with oxidizing substance
2+) and hydroxyl (combination OH) has been stablized (Fe
2+), when being handled, this " functional peptide-Fe " material exempts from oxidation under spray drying and other non-vacuum conditions.(providing OH) is former, and ethanol is optimum selection as hydroxyl;
(3) the flocculation recovery technology of blood " functional peptide-Fe "
After technology such as the vacuum degraded of hemoglobin process the present invention (two) bar hemoglobin of animal and ionization electrolysis were decomposed, formation comprised heavy metal ion, aminoacid, the short peptide chain that contains in various salt ions, the blood, the mixture that also comprises the complexity of " functional peptide-Fe ".If the peptide that obtains being correlated with-Fe product, need carry out ion sieve and separate in the middle of technology in the past, electronic disengaging or dialysis mode break away from method such as son and separate and make with extra care, both spended time, cost cost height, and the while influenced purity again.The present invention develops simple and easy and the recovery technology of flocculating accurately according to the physicochemical property of " functional peptide-Fe ", can from the complex of complexity, optionally " functional peptide-Fe " flocculation sediment be come out, by centrifugalize or filtration, can obtain the very high end product of purity " functional peptide-Fe ".As the flocculation recovery catalyst water-soluble chitosan and the stronger Organic substance of polarity are arranged, but, though this type of material can make " functional peptide-Fe " flocculation and precipitate, but also get along well " functional peptide-Fe " coprecipitation, do not influence the purity and the quality of " functional peptide-Fe " after the centrifugalize.
(4) medical value of " functional peptide-Fe " and health care
1. the antioxidation of " functional peptide-Fe " and the function of eliminating free radical
Hydrogen peroxide (H
2O
2) be the peroxidating material that organism (cell) inevitably can produce when carrying out metabolism, if it is untimely in vivo removing will damage tissue and cell, particularly more outstanding to the injury of cardiovascular and cerebrovascular vessel.Find among the present invention and should " functional peptide-Fe " have antioxidation, eliminate the hydrogen peroxide effect; (H
2O
2) elimination mainly realize by ferrous ion in " functional peptide-Fe " and hydroperoxidation.
2. " functional peptide-Fe " can remove the destruction of hydroxyl radical free radical (OH) to collagen protein
In the ordinary course of things, inorganic two iron ions can produce hydroxyl radical free radical (OH) with hydroperoxidation.Up to the present, the destruction to tissue and cell etc. of hydroxyl radical free radical (OH) is the strongest in all peroxide of finding.The present invention's discovery, after inorganic ferrous ion runs into hydrogen peroxide, can be totally with the instantaneous digestion of collagen molecules, and, under the certain situation of concentration of hydrogen peroxide, along with the increase of ferrous ion concentration, to the destruction also big more (Fig. 2) of collagen molecules.S is a molecular weight marker albumen among Fig. 2; Article 1, protein band is standard collagen protein, 300 μ g/ml; The the 2nd to the 11st electrophoresis band is under the concentration of same collagen protein, adds the hydrogen peroxide of 10mM, adds ferrous sulfate (ferrous ion) 0.0～0.5mM more successively.Simple hydrogen peroxide can not destroy collagen molecules (Fig. 2-2), but when in the solution that contains hydrogen peroxide, adding ferrous ion, hydrogen peroxide will generate hydroxyl radical free radical (this also frequent in vivo phenomenon) with the ferrous ion reaction, thereby collagen protein is produced the decomposability lethal effect, and under the certain condition of concentration of hydrogen peroxide, along with the increase damage capability of ferrous ion concentration also strengthens, when ferrous ion reaches 0.5mM, the collagen molecules of 300 μ g/ml all be decomposed (Fig. 2-11).
But, when substituting the ferrous sulfate of inorganic iron with same ferrous ion concentration, will eliminate (Fig. 3) fully to the destruction of collagen protein with " functional peptide-Fe ".Proof " functional peptide-Fe " can be protected collagen protein from hydroxyl radical free radical.Among Fig. 3, S is a molecular weight marker; 1-3 is " functional peptide-Fe " (the calculating with ion concentration) of adding 0.5mM; 4-6 is the ferrous sulfate that adds 0.5mM.
3. prevent and treatment anemia medicinal function
Iron deficiency anemia at home and abroad is very general, national nutriture investigation according to Japanese health ministry shows: there is the iron deficiency of potentiality in the people of the 30%-40% of population, there is the people of 8%-13% to have tangible iron deficiency anemia, particularly juvenile and the women is particularly outstanding.China shows at the investigation report by Beijing Medical University in 1998: anemia patient male is up to 42% for 7 years old child of China (only as example), and the women is up to 45%.Child's prevalence below 6 years old reaches 40%.Calculate in this ratio, China probably has 4-5 hundred million populations to be in iron deficiency (or iron deficiency anemia) state.In the checking of " functional peptide-Fe " of the present invention medical value, by evidence to the anemia patient, take a week above (a day dosing is 12mg, the adult) continuously, Anemia (dizziness, mucosal pallor, cardiopalmus, easily tired, headache, weakness etc.) disappears substantially or is clearly better.And take for a long time or untoward reaction such as nausea and vomiting do not appear in excessive use yet, this is and one of ionic important difference of inorganic iron.
4. the good absorption of " functional peptide-Fe "
In a single day inorganic salts is eaten excessively just has a side effect such as nauseating, vomiting, in addition, also is subjected to multiple factor affecting in the food on absorbing, thereby causes absorbance extremely low, generally only has about 5-7%.Peptide-the Fe or the ferrous porphyrin that have the blood origin only are the organic ferrum of natural biological, prove nontoxic having no side effect in many ways, the influence of compositions such as cellulose, carbonate, hydroxyl in the unable to take food thing.Iron ion absorption test by Kunming white mouse proves that the absorbance of " functional peptide-Fe " is the ionic several times of inorganic iron up to 37%.
(5) clearer production technology of " functional peptide-Fe "
The present invention has also paid attention in the blood deep-processing process protection and secondary pollution to environment except the deep process of paying attention to " functional peptide-Fe " product.Exploitation by airtight technology, vacuum concentration and spray drying, fully utilize and exploitation etc., make blood processing obtain nearly connecting to neutral discharging according to blood constituent different.
Five, concrete application example of the present invention
1. " functional peptide-Fe " capsule: adopt gastric-juice-soluble " functional peptide-Fe " to be prepared into capsule, wherein every capsules contains " functional peptide-Fe " 1g, in treatment anemia man-hour, obeys four every day; When being used to prevent anemia, obey 2 every day;
2. " functional peptide-Fe " sugar coated tablet: adopt gastric-juice-soluble " functional peptide-Fe " to be prepared into sugar coated tablet, wherein every contains " functional peptide-Fe " 0.5g, is suitable for daily iron supplement of child and old people and prevention anemia, obeys the 4-8 grain every day;
3. " functional peptide-Fe " instant granular electuary: adopt water solublity " functional peptide-Fe " through being equipped with auxiliary and condiment and, being prepared into " functional peptide-Fe " instant granular electuary through pelletize (grain graininess is within 1mm) technology.Can be used for treatment and prevention iron deficiency anemia patient, or be used for daily iron supplement;
4. " functional peptide-Fe " chocolate; Adopt water solublity " functional peptide-Fe " to be prepared into " functional peptide-Fe " chocolate with the form of additive, its content is at 1-2%, as children special-purpose iron supplement product;
5. " functional peptide-Fe " sandwich biscuit: adopt gastric-juice-soluble " functional peptide-Fe " to use the preparation technology of sandwich biscuit, can be used to prepare the functional food of iron supplement, prevention anemia; Especially for the old people, and breakfast iron supplement product;
6. " functional peptide-Fe " enriched soy sauce: " functional peptide-Fe " self physicochemical property and soy sauce and similar, and meet hot equistability might as well, be well suited for preparing the iron supplement soy sauce.
Description of drawings
The vacuum of Fig. 1, hemoglobin-ionization electrolysis sketch map
Fig. 2, hydrogen peroxide and inorganic ferrous ion are to the destruction of collagen molecules
S: standard protein; 1: the collagen protein reference material; 2～11: the concentration of ferrous ion (FeSO4) increases successively that (0.0mM～0.5mM) concentration of hydrogen peroxide is 10mM.Collagen concentration is 300ug/ml.
Fig. 3, " functional peptide-Fe " and of the comparison of inorganic ferrous ion to the collagen protein protective effect
S: standard protein; 1: the collagen protein reference material; 1～3: " functional peptide-Fe "; Iron concentration is for time being (0.05,0.3,0.5mM) 4～6: the concentration of inorganic ferrous ion (FeSO4) is complied with.Other and Fig. 2 are together.
Fig. 4, processing technology routine.
Claims (3)
- Utilize the method for the blood (hemoglobin) of animal through ionization electrolysis under vacuum condition and flocculation recovery technology production " functional peptide-Fe ".It is characterized in that: animal blood is through vacuum cutter blood sampling and carry out dynamic anticoagulation and handle, and the immunological probe that carries out eqpidemic disease in the time of blood sampling detects, and the blood of safety and Health is recovered to the seal storage jar as the utilization of blood rapidoprint; Centrifugal (or through sedimentation tower precipitation) separating red corpuscle, and carry out haemolysis (all can make the hemolytic method of erythrocyte fragmentation hypotonic haemolysis, ultrasound wave haemolysis, chemical haemolysis etc.) and handle; Hemoglobin behind the haemolysis placed carry out electrolysis in the electrolysis bath with vacuum action, under the reduction of forceful electric power, hemoglobin is cracked into small-molecular peptides, and comprising " functional peptide-Fe " that contain ferrous porphyrin, its molecular weight distribution is between 1000～2000Da; The micromolecule that obtains after the ionization electrolysis " functional peptide-Fe ", through in the electrolysis bath lysate, adding flocculating agent, make " functional peptide-Fe " flocculation sediment from the catabolite of complexity, centrifugal or filtration is optionally reclaimed also and is obtained " functional peptide-Fe " after the drying; " functional peptide-Fe " is the organic ferrum of natural biological with good absorption, no side effects, have iron supplement, treatment anemia and the effect of excellent prevention anemia is arranged, have antioxidation simultaneously, eliminate effects such as free radical and prevention cardiovascular and cerebrovascular disease.This invention specifically comprises following content:1, the auxiliary blood sampling Technology of preparation pharmaceutical grade " functional peptide-Fe ":1) vacuum cutter blood collecting technology: select for use by vacuum pump, closed conduit, circle or oblate vacuum blood sampling knife, blood holding vessel at interior vacuum cutter blood sampling system, animal is carried out (blood sampling) blood-letting, with air and the complete isolated condition of external environment under gather blood in hermetic container, to guarantee the extraneous contact of blood discord, to make blood exempt from secondary pollution;2) the dynamic anticoagulant technology of blood: more than the 20cm at distance vacuum cutter tip, locate to be communicated with the liquid anticoagulant by tubule, and according to the flow of blood and the consumption of flow velocity auto-control anticoagulant, to obtain the best anticoagulant effect of minimum dose;3) the Active immunity probe in detecting technology of blood: the antibody of the infecting both domestic animals and human eqpidemic disease that the preparation animal is common is also made probe, is placed in the blood holding vessel, in case detect eqpidemic disease, blood is handled at once, guarantees the safety of blood in the whole gatherer process.
- 2, the technology of preparing of " functional peptide-Fe ":1) the ionization electrolysis tech of hemoglobin: through 1 1), 2), what 3) item obtained is fresh, the animal blood of safety, obtain erythrocyte and further make it haemolysis by centrifugalize, separation is removed cell wall and is obtained hemoglobin, hemoglobin is put into the anode chamber of tank house, electrolyte is NaCl or KCl or the arbitrary proportion mixture of the two, the a large amount of electronics of energising back anode accumulation, cause the strong reducing property environment, hemoglobin begins to be cracked into small peptide with this understanding, obtain containing the small peptide of ferrous porphyrin simultaneously, be called " functional peptide-Fe ", its molecular weight is at 1000～2000Da;2) the indirect ionization electrolysis tech of hemoglobin: as the expansion of above-mentioned ionization electrolysis tech, directly do not use electrolysis bath, and be to use with NaCl or KCl or the mixture of the two is the electrolyte of preparation of electrolyte, handle hemoglobin indirectly, can obtain identical effect equally, therefore, the same application of the present invention is to the protection of indirect electrolytic technology;3) the vacuum degradation technique of hemoglobin: two effects are greatly played in the vacuum degraded in the present invention: the one, and protection ferrous ion, the bivalence (Fe in the ferrous porphyrin of hemoglobin 2+) ion easily is oxidized to ferric ion (Fe touching under the oxidizing conditions such as oxygen 3+), thereby lose its biological value, reduce its absorbability simultaneously, so adopt the ionization electrolytic process under the vacuum condition among the present invention, the oxidizing substances that produce in isolation oxygen and the electrolytic process etc. are to realize protecting fully bivalence (Fe 2+) ionic purpose; The 2nd, by vacuum condition, accelerate between the material, contacting between hemoglobin and the electronics particularly, thereby accelerate course of reaction, reduce production costs.4) the flocculation recovery technology of " functional peptide-Fe ": to through 1 in the claim two), 2) " functional peptide-Fe " and other complex for preparing after the cracking of vacuum ionization electrolysis process process (comprises the salt in the blood, Organic substance, small peptide, heavy metal etc.) add an amount of in, suitable flocculating agent, as water-soluble chitosan, the isopolarity Organic substance, make " functional peptide-Fe " specifically from numerous complex flocculation sediment come out, through centrifugal or filtered and recycled, thereby can remove irrelevant composition, particularly unhelpful heavy metal class material to health, purified to obtain " functional peptide-Fe ".5) resist technology of ferrous ion in " functional peptide-Fe ": in order to block ferrous ion (Fe in " functional peptide-Fe " 2+) with the touch opportunity of oxidizing substance, the present invention develops utilization (Fe 2+) and hydroxyl (combination OH) has been stablized (Fe 2+), make this " functional peptide-Fe " material when spray drying, exempt from oxidation.As hydroxyl (OH) the source is provided, ethanol is optimum selection;6) the present invention is adapted to the processing of all animal bloods: comprise fowl blood such as Sanguis sus domestica, Sanguis caprae seu ovis, Sanguis Bovis seu Bubali, horse blood, donkey blood, Sanguis cervi, chicken and duck goose.
- 3, the medical value of " functional peptide-Fe ", health care, purposes and application1) " functional peptide-Fe " material derives from hemoglobin in the animal erythrocyte, as the organic ferrum of natural biological, its absorbance can reach (inorganic iron can only absorb 7%) more than 35%, be present absorbance the highest, with the best organic ferrum of affinity of human body, and without any side effects.Therefore, this material is the best medical substance of treatment iron deficiency anemia, and it can be developed to medical products such as " functional peptide-Fe " capsule, " functional peptide-Fe " instant granular, " functional peptide-Fe " tablet;2) " functional peptide-Fe " of the present invention's preparation can be prepared into two kinds of water solublity " functional peptide-Fe " and gastric juice dissolubilities " functional peptide-Fe " as required, can improve the approach that utilizes of " functional peptide-Fe " widely, helps the processing of product; Except that last listed medical value be developed to the medical product, also can be used as functional food additives based on iron supplement, prevention and treatment anemia etc., also can be used as feed additive;3) function of the antioxidation of " functional peptide-Fe " and elimination free radical: the present invention finds that " functional peptide-Fe " can very fast elimination hydrogen peroxide (H 2O 2) and bodily tissue, cell, DNA, collagen protein etc. are had the hydroxyl radical free radical (OH) of extremely strong lethal effect, thus body and each tissue and organ are played a protective role.Utilize this function can develop the antioxidation of " functional peptide-Fe " and medicine, health product and the varieties of food items additive of prevention cardiovascular and cerebrovascular disease.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101664543B (en) * | 2009-09-02 | 2011-10-26 | 张滨 | Hemokinin-iron oral liquid |
| CN107509916A (en) * | 2017-08-07 | 2017-12-26 | 天津科技大学 | The method that nitrosohaemoglobin peptide is prepared using pig blood |
| CN114717057A (en) * | 2022-03-18 | 2022-07-08 | 九江德福科技股份有限公司 | Cleaning solution and cleaning method for cleaning collagen adding system |
-
2003
- 2003-06-13 CN CNA031488269A patent/CN1605346A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101664543B (en) * | 2009-09-02 | 2011-10-26 | 张滨 | Hemokinin-iron oral liquid |
| CN107509916A (en) * | 2017-08-07 | 2017-12-26 | 天津科技大学 | The method that nitrosohaemoglobin peptide is prepared using pig blood |
| CN114717057A (en) * | 2022-03-18 | 2022-07-08 | 九江德福科技股份有限公司 | Cleaning solution and cleaning method for cleaning collagen adding system |
| CN114717057B (en) * | 2022-03-18 | 2023-12-12 | 九江德福科技股份有限公司 | Cleaning liquid and cleaning method for cleaning collagen adding system |
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