CN1666633A - Production method of nutritious fungus feed addictive - Google Patents
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Abstract
The invention relates to the production method of paecilomyces cicadae new nutritious fungus feed addictive. The strain used in the method is traditional chinese herbal medicine paecilomyces cicadae. The process includes production processes such as acquisition of strain, cultivation of slant strains and liquid seeds, liquid fermentation, cultivation of solid substrate and so on. The result of animal test indicates that the fungus feed addictive can not only improve the feed conversion rate, save cost, gain meat animals' weight more quickly, reduce the cost by way of improving animal's immunological competence and survival rate with few medicine or no medicine, but also can achieve good effect on weight gains with little quantity of addictive. The production of the fungus feed addictive has advantages of wide raw material sources, easy production, low cost, controllable quality, convenient use, wide application, good actual effect and so on.
Description
Technical field
The present invention relates to a kind of production method of nutritious fungus feed addictive, the making method of the novel fodder additive of use specifically that Paecilomyces cicadae (Paecilomyces cicadae (Mip.) Samson) is cultivated, liquid fermenting and solid substrate being cultivated.
Background technology
The non-nutritive additive that is adopted in the feed mostly is microbiotic, chemical synthetic drug, hormone etc. at present, they are residual in animal body easily, life-time service, cultivated animals is developed immunity to drugs or remain in the finished product (meat, egg, milk etc.) of cultivated animals, directly or indirectly HUMAN HEALTH is worked the mischief, cause poisoning, resistance and " three cause " (carcinogenic, teratogenesis, mutagenesis) effect.Green feed additive has been represented future thrust, be meant pollution-free, noresidue, anti-disease, somatotrophic natural additive in broad terms, at present, dissimilar additives such as Chinese herbal and crude drugs preparations, zymin, microbial preparation, souring agent, mould inhibitor, oligose, sugared terpene, garlic have been developed.
Chinese herbal feed additive had both contained nutritive substance, have the calmness of calming the nerves again, the digestion promoting that is good for the stomach, vital energy regualting and blood circulation-promoting, support smart help, worm health care and pharmacological action such as prevent and cure diseases become; Herbal medicine immunologic active material energy enhancing body immunity function improves animal anti-stress, anti-disease ability, improves breeding performonce fo animals, and has noresidue, is difficult for producing advantages such as resistance, is a big focus of international animal trophology research in recent years.Herbal medicine can solve the antibiotic remains problem of long-term puzzlement animal husbandry development, boosts productivity, and reduces the pollution of livestock industry to environment, develops green livestock industry, satisfies the people's food safety demand.
Though the research and development of Chinese herbal feed additive product have obtained certain progress, and the control to livestock and poultry has obtained effect preferably within the specific limits, but also there are many problem demanding prompt solutions, big as the product addition, effective constituent is not clear, the mechanism of action is unclear, the action effect unstable, formulation is single, lack the research of toxicity secure context, the quality control standard of raw material and product is incomplete etc., causes new Chinese herb feed additive research and development dynamics little, and the listing product innovation is less.The existence of these problems makes in the market Chinese herbal feed additive not meet the basic function principle of " trace, efficient " this fodder additives, is difficult to realize industrialization.
With the closely-related Cordyceps nutrition type feed additive of Chinese herbal feed additive be wherein treasure, it had both solved it and had been difficult for the difficult point of industrialization, toxicological security research shortage, and addition is little, nutritive ingredient is more complete, and because the applicating history of Chinese caterpillar fungus in popular life more easily accepted by the masses, as long as solve the difficult point of production technique, reduce productive expense, very easily application.
Have only three Chinese patents more approaching with this patent are that CN97107712.6 and other two are the Cordyceps militaris (L.) Link. mycelia and are used for fodder additives, but difference is more obvious.The former is that mainly this patent involvement aspect is too wide, it is hundreds of to relate to known and unknown Chinese caterpillar fungus teleomorph and anamorph fungi thereof, its liquid of different Cordyceps fungus is different with solid fermentation process, its ingredient is not quite similar, function also emphasizes particularly on different fields, some Chinese caterpillar fungus is of value to animal health, and some is not only unhelpful harmful on the contrary, as the stinkbug Chinese caterpillar fungus, the activeconstituents of Chinese caterpillar fungus and the anamorph thereof of knowing for sure at present and the kind of nourishing function are seldom, only account for 5% of all Chinese caterpillar fungus kinds approximately, more kind is if want to be applied to the research that animal health is still needed and will be goed deep into.The embodiment of this patent paecilomyces Paecilomyces spp., its problem is, this is a very big genus, mean to comprise all Paecilomyces variotis, and some Paecilomyces varioti can be used for animal health, and some can not be used for animal health, promptly enable, gene difference is not bigger between of the same race, and its fermentation condition, ingredient and pharmacologically active all have difference, and application also can be different.Both bacterial strain uses therefors of back are the pupa Paecilomyces varioti, are not same kinds.In a word, (1) used bacterial classification difference; (2) culture medium prescription difference; (3) production technique difference.
Summary of the invention
In order to address the above problem, the invention provides and a kind of Paecilomyces cicadae (Paecilomyces cicadae (Mip.) Samson) is carried out spawn culture, optimize the production method of the nutritious fungus feed addictive that liquid fermenting and solid substrate cultivate.
Realize that the concrete technical solution of above-mentioned purpose is as follows:
1, a kind of production method of nutritious fungus feed addictive comprises that bacterial classification is obtained, spawn culture, strain fermentation and solid culture, and described bacterial classification is Paecilomyces cicadae (Paecilomyces cicadae (Mip.) Samson), and this bacterial classification is a cosmopolitan species; Paecilomyces cicadae distributes wide, sees the adult of cicada, can be used as medicine, and is called " cicada fungus ", in " entomomycete " that Anhui science tech publishing house publishes detailed introduction is arranged, and it is characterized in that:
(1) described spawn culture comprises slant tube spawn culture and two steps of liquid shaking bottle seed culture;
(2) fermentation of described bacterial classification comprises first class seed pot fermentation and secondary seed jar fermentation two-stage liquid strain fermentation;
(3) described solid culture adds an amount of water for after solid materials is mixed, and its weight part is a material: water=5: 1; Mix thoroughly in the cloth bag of packing into and sterilize; Then inoculation, cultivate finished product.
2, according to the production method of above-mentioned 1 described a kind of nutritious fungus feed addictive: concrete processing step and condition are as follows:
(1) described spawn culture was divided into for two steps:
A, slant tube spawn culture
The bacterial classification inoculation of separation and purification in slant tube, is put into 25 ℃ of incubators, and incubation time is 7 days, treats that mycelia is covered with test tube and produces spore to get final product;
B, liquid shaking bottle seed culture
The 200ml substratum of packing in the 500ml triangular flask is used 1.2Kg/cm
2Autoclaving 30 minutes, to be cooled during to room temperature, will be to 500ml triangular flask substratum through the bacterial classification inoculation of 1 slant tube, and place the constant-temperature shaking culture case, and 25 ± 1 ℃ of temperature are cultivated under the 180r/m condition, and incubation time is 72h～96h;
(2) fermentation of described bacterial classification is the second-class liquid isolate fermentation:
A, first class seed pot production
The 21L liquid nutrient medium of in the 50L airlift fermentor, packing into, the substratum temperature is 6.0～7.0 greater than 95 ℃, pH value, and adds edible defoaming agent, add-on is 0.03%, 14.7 * 10
4Under the Pa pressure, feed steam heating to 121 ℃, and pressurize 30～45 minutes; After the sterilization, when being cooled to 20 ℃ 4 bottles of above-mentioned cultured 500ml shake-flask seeds are inserted cultivation, culture temperature is 25～26 ℃, and air flow is 1:0.5V/Vmin, pressurize 4.903～7.0845 * 10
4Pa through the 48h aerated culture, reaches the logarithmic growth after date and gets final product, and final volume of culture is 30L;
B, secondary seed jar are produced
The 300L liquid nutrient medium of in the 500L seeding tank, packing into, the pH value is 6.0～7.0, and adds edible defoaming agent, and add-on is 0.03%, and logical steam and original position are heated to 121 ℃, 14.7 * 10
4Under the Pa pressure, pressurize 30～45 minutes; After the sterilization, when being cooled to 26 ℃, the 30L liquid seeds of first class seed pot all being inserted the secondary seed jar cultivate, 25～26 ℃ of culture temperature, air flow is 1:0.5V/Vmin, pressurize 4.903～7.0845 * 10
4Pa through the 48h aerated culture, reaches the logarithmic growth after date and gets final product, and final volume of culture is 300L;
(3) solid substrate of described bacterial classification is cultivated and is carried out as follows:
After solid materials mixed, add an amount of water, its weight part is a material: water=5: 1; Mix sterilization (employing tyndallization) in the cloth bag of packing into thoroughly.Solid medium after the sterilization is put in the clean pallet, is positioned over and is cooled to 24 ℃～25 ℃ inoculum sizes in the aseptic ventilating chamber and inserts liquid seeds with 8%.It is about 54% to mix final water ratio that the back guarantees material thoroughly, postvaccinal material is tiled in the thickness of 3cm puts into culturing room in the tray and cultivate.Temperature remain on 24 ℃～25 ℃ humidity remain on 95% cultivate 72h after, temperature is that 24 ℃～25 ℃ humidity drop to 85% and continue to cultivate and got final product discharging in 3～4 days.Oven dry or dry, pulverize, get product behind mistake 300 orders.
3, according to the production method of above-mentioned 2 described a kind of nutritious fungus feed addictives, it is characterized in that: the raw material weight proportioning of described slant tube bacterium culture medium is: glucose 40g, peptone 10g, yeast extract powder 10g, agar 20g, add water to 1L, pH6.5.
4, according to the production method of above-mentioned 2 described a kind of nutritious fungus feed addictives, it is characterized in that: described liquid shaking bottle seed culture medium raw material weight proportioning is: analysis for soybean powder 20g, white sugar 30g, yeast powder 5g, MgSO
40.5g, KH
2PO
40.5g, add water to 1L, pH6.5.
5, according to the production method of above-mentioned 2 described a kind of nutritious fungus feed addictives, it is characterized in that: described first class seed pot liquid medium starting material weight proportion is: contain white sugar 30g, analysis for soybean powder 30g, MgSO in the 1000ml substratum
40.5g, KH
2PO
41g adds water after each component is mixed and mends to 1000ml, and is 6.5 with saturated NaOH adjust pH.
6, according to the production method of above-mentioned 2 described a kind of nutritious fungus feed addictives, it is characterized in that: described secondary seed jar liquid medium starting material weight proportion is: contain white sugar 30g, analysis for soybean powder 30g, MgSO in the 1000ml substratum
40.5g, KH
2PO
41g adds water after each component is mixed and mends to 1000ml, and is 6.5 with saturated NaOH adjust pH.
7, according to the production method of above-mentioned 2 described a kind of nutritious fungus feed addictives, it is characterized in that: described defoamer is a polyoxypropylene ethylene oxide glyceryl ether, and consumption is 0.03% of a nutrient solution total amount.
8, according to the production method of above-mentioned 2 described a kind of nutritious fungus feed addictives, it is characterized in that: the solid substrate culture medium raw material weight proportion of described bacterial classification is: contain Semen Maydis grit 750g, bean cake powder 200g, wheat bran 50g, inorganic salt 2g in the 1000g substratum.
9, according to the production method of above-mentioned 2 described a kind of nutritious fungus feed addictives, it is characterized in that: the solid substrate culture process is a solid materials: water=5: 1, adopt tyndallization, during sterilising treatment solid materials is heated to 100 ℃, continue 20-45 minute, once a day, for three days on end, the last processing, but heat-up time proper extension.Be cooled to 24 ℃～25 ℃ inoculum sizes and insert liquid seeds with 8%.It is about 54% to mix final water ratio that the back guarantees material thoroughly, postvaccinal material is tiled in the thickness of 3cm puts into culturing room in the tray and cultivate.Temperature remain on 24 ℃～25 ℃ humidity remain on 95% cultivate 72h after, temperature is that 24 ℃～25 ℃ humidity drop to 85% and continue to cultivate discharging after 3～4 days; Oven dry or dry, pulverize, get product behind mistake 300 orders.
Production method of the present invention is fit to suitability for industrialized production, is not subjected to the limitation in time place, can be according to the increase in demand or the reduction industrial scale in market.Production technique of the present invention is simple, process stabilizing, easy-regulating, success ratio height; Adopt the inventive method production less investment, take up an area of little, no three wastes problem, be suitable for large and medium-sized enterprise and adopt.Its product is a kind of fodder additives of functional type, can be used as the protective foods of animals such as livestock and poultry fish.
The nutrition type feed additive that the present invention makes by liquid and the used Paecilomyces cicadae of solid fermentation body-care is the more potential fodder additives of a class, it is different from the microorganism live bacteria preparation, can regard Chinese herbal feed additive as in itself, but it has overcome the shortcoming of herbal medicine, keeps even surpass its advantage.Not only can improve food conversion ratio and save cost, faster increase meat animals body weight and improve output, and can under the prerequisite of not medication or few medication, reduce cost because of the immunoregulation capability that strengthens animal improves its surviving rate.
The nutrition type feed additive that the inventive method is produced has unique advance: one, used bacterial classification is traditional herbal medicine Paecilomyces cicadae; Be applied to animal health still product-free appear on the market, have leading consciousness and top standard, its security simultaneously and validity are easy to be accepted by the people and approve; Two, animal test results shows: not only improve food conversion ratio and save cost, faster increase meat animals body weight and improve output, and can under the prerequisite of not medication or few medication,, the immunoregulation capability that strengthens animal reduce cost because of improving its surviving rate, only need addition seldom just can reach good gaining effect, as the comparable contrast weightening finish 15%～30% of 0.25% addition; This fungi fodder additives has disease-resistant significantly, growth promoting function, and have that toxic side effect is little, drug residue free, advantage such as have no drug resistance, be wide spectrum of new generation, efficient green fodder additives; Three, produce this fungi fodder additives also have raw material sources wide, easily produce, with low cost, quality controllable, easy to use, be widely used and characteristics such as actual effect is good; Four, the mycelia of solid culture and be secreted into the activeconstituents of residue in the culture medium and be used as additive raw material together, thus maximally utilise resource, pursue bigger economic and social benefit, and pollution-free.
Embodiment
In conjunction with the embodiments the present invention is done to describe further.
Embodiment:
The production method of this nutritious fungus feed addictive comprises following production process:
Obtaining of A, bacterial classification
Paecilomyces cicadae bacterial classification (Paecilomyces cicadae (Mip.) Samson) is cosmopolitan species.
B, slant tube spawn culture
The bacterial classification inoculation of separation and purification in slant tube, is put into 25 ℃ of incubators, and incubation time is 7 days, treats that mycelia is covered with test tube and produces spore to get final product;
The raw material weight proportioning of slant tube bacterium culture medium is: glucose 40g, peptone 10g, yeast extract powder 10g, agar 20g add water to 1L, pH6.5.
C, liquid shaking bottle seed culture
The 200ml substratum of packing in the 500ml triangular flask is used 1.2Kg/cm
2Autoclaving 30 minutes, to be cooled during to room temperature, will be to 500ml triangular flask substratum through the bacterial classification inoculation of 1 slant tube, and place the constant-temperature shaking culture case, and 25 ± 1 ℃ of temperature are cultivated under the 180r/m condition, and incubation time is 72h～96h;
Liquid shaking bottle seed culture medium raw material weight proportioning is: analysis for soybean powder 20g, white sugar 30g, yeast powder 5g, MgSO
40.5g, KH
2PO
40.5g, add water to 1L, pH6.5.
D, first class seed pot production
The 21L liquid nutrient medium of in the 50L airlift fermentor, packing into, the substratum temperature is 6.0～7.0 greater than 95 ℃, pH value, and adds edible defoaming agent, add-on is 0.03%, 14.7 * 10
4Under the pa pressure, feed steam heating to 121 ℃, and pressurize 30～45 minutes; After the sterilization, when being cooled to 20 ℃ 4 bottles of above-mentioned cultured 500ml shake-flask seeds are inserted cultivation, culture temperature is 25～26 ℃, and air flow is 1:0.5V/Vmin, pressurize 4.903～7.0845 * 10
4Pa through the 48h aerated culture, reaches the logarithmic growth after date and gets final product, and final volume of culture is 30L;
First class seed pot liquid medium starting material weight proportion is: contain white sugar 30g, analysis for soybean powder 30g, MgSO in the 1000ml substratum
40.5g, KH
2PO
41g adds water after each component is mixed and mends to 1000ml, and is 6.5 with saturated NaOH adjust pH.
E, secondary seed jar are produced
The 300L liquid nutrient medium of in the 500L seeding tank, packing into, the pH value is 6.0～7.0, and adds edible defoaming agent, and add-on is 0.03%, and logical steam and original position are heated to 121 ℃, 14.7 * 10
4Under the Pa pressure, pressurize 30～45 minutes; After the sterilization, when being cooled to 26 ℃, the 30L liquid seeds of first class seed pot all being inserted the secondary seed jar cultivate, 25～26 ℃ of culture temperature, air flow is 1:0.5V/Vmin, pressurize 4.903～7.0845 * 10
4Pa through the 48h aerated culture, reaches the logarithmic growth after date and gets final product, and final volume of culture is 300L;
Secondary seed jar liquid medium starting material weight proportion is: contain white sugar 30g, analysis for soybean powder 30g, MgSO in the 1000ml substratum
40.5g, KH
2PO
41g adds water after each component is mixed and mends to 1000ml, and is 6.5 with saturated NaOH adjust pH.
The solid substrate of F, bacterial classification is cultivated and is carried out as follows:
In the 3500kg substratum, contain Semen Maydis grit 2625kg, bean cake powder 700kg, wheat bran 175kg, inorganic salt 70kg.After solid materials mixed, add an amount of water 700kg (material: water=5: 1) mix thoroughly in the cloth bag of packing into and sterilize; Adopt tyndallization, during sterilising treatment solid materials be heated to 100 ℃, continue 20-45 minute, once a day, for three days on end, the last processing, but heat-up time proper extension.Solid medium after the sterilization is put in the clean pallet, is positioned over and is cooled to 24 ℃～25 ℃ inoculum sizes in the aseptic ventilating chamber and inserts liquid seeds with 8%.It is about 54% to mix final water ratio that the back guarantees material thoroughly, postvaccinal material is tiled in the thickness of 3cm puts into culturing room in the tray and cultivate.Temperature remain on 24 ℃～25 ℃ humidity remain on 95% cultivate 72h after, temperature is that 24 ℃～25 ℃ humidity drop to 85% and continue to cultivate and got final product discharging in 3～4 days.Oven dry or dry, pulverize, cross 300 orders after carry out the final product quality check, qualified back pack product.
Claims (9)
1, a kind of production method of nutritious fungus feed addictive, comprise that bacterial classification is obtained, spawn culture, strain fermentation and solid culture, described bacterial classification is Paecilomyces cicadae bacterial classification (Paecilomyces cicadae (Mip.) Samson), and this bacterial classification is a cosmopolitan species; It is characterized in that:
(1) described spawn culture comprises slant tube spawn culture and two steps of liquid shaking bottle seed culture;
(2) fermentation of described bacterial classification comprises first class seed pot fermentation and secondary seed jar fermentation two-stage liquid strain fermentation;
(3) described solid culture adds an amount of water for after solid materials is mixed, and its weight part is a material: water=5: 1; Mix thoroughly in the cloth bag of packing into and sterilize; Then inoculation, cultivate finished product.
2, the production method of nutritious fungus feed addictive according to claim 1 is characterized in that:
(1) described spawn culture was divided into for two steps:
A, slant tube spawn culture
The bacterial classification inoculation of separation and purification in slant tube, is put into 25 ℃ of incubators, and incubation time is 7 days, treats that mycelia is covered with test tube and produces spore to get final product;
B, liquid shaking bottle seed culture
The 200ml substratum of packing in the 500ml triangular flask is used 1.2Kg/cm
2Autoclaving 30 minutes, to be cooled during to room temperature, will be to 500ml triangular flask substratum through the bacterial classification inoculation of 1 slant tube, and place the constant-temperature shaking culture case, and 25 ± 1 ℃ of temperature are cultivated under the 180r/m condition, and incubation time is 72h～96h;
(2) fermentation of described bacterial classification is the second-class liquid isolate fermentation:
A, first class seed pot production
The 21L liquid nutrient medium of in the 50L airlift fermentor, packing into, the substratum temperature is 6.0～7.0 greater than 95 ℃, pH value, and adds edible defoaming agent, add-on is 0.03%, 14.7 * 10
4Under the Pa pressure, feed steam heating to 121 ℃, and pressurize 30～45 minutes; After the sterilization, when being cooled to 20 ℃ 4 bottles of above-mentioned cultured 500ml shake-flask seeds are inserted cultivation, culture temperature is 25～26 ℃, and air flow is 1: 0.5V/Vmin, pressurize 4.903～7.0845 * 10
4Pa through the 48h aerated culture, reaches the logarithmic growth after date and gets final product, and final volume of culture is 30L;
B, secondary seed jar are produced
The 300L liquid nutrient medium of in the 500L seeding tank, packing into, the pH value is 6.0～7.0, and adds edible defoaming agent, and add-on is 0.03%, and logical steam and original position are heated to 121 ℃, 14.7 * 10
4Under the Pa pressure, pressurize 30～45 minutes; After the sterilization, when being cooled to 26 ℃, the 30L liquid seeds of first class seed pot all being inserted the secondary seed jar cultivate, 25～26 ℃ of culture temperature, air flow is 1: 0.5V/Vmin, pressurize 4.903～7.0845 * 10
4Pa through the 48h aerated culture, reaches the logarithmic growth after date and gets final product, and final volume of culture is 300L;
(3) solid substrate of described bacterial classification is cultivated and is carried out as follows:
After solid materials mixed, add an amount of water, its weight part is a material: water=5: 1; Mix thoroughly in the cloth bag of packing into and sterilize; Solid medium after the sterilization is put in the clean pallet, is positioned over and is cooled to 24 ℃～25 ℃ inoculum sizes in the aseptic ventilating chamber and inserts liquid seeds with 8%; It is about 54% to mix final water ratio that the back guarantees material thoroughly, postvaccinal material is tiled in the thickness of 3cm puts into culturing room in the tray and cultivate; Temperature remain on 24 ℃～25 ℃ humidity remain on 95% cultivate 72h after, temperature is that 24 ℃～25 ℃ humidity drop to 85% and continue to cultivate and got final product discharging in 3～4 days; Oven dry or dry, pulverize, get product behind mistake 300 orders.
3, the production method of nutritious fungus feed addictive according to claim 2, it is characterized in that: the raw material weight proportioning of described slant tube bacterium culture medium is: glucose 40g, peptone 10g, yeast extract powder 10g, agar 20g, add water to 1L, pH6.5.
4, the production method of nutritious fungus feed addictive according to claim 2 is characterized in that: described liquid shaking bottle seed culture medium raw material weight proportioning is: analysis for soybean powder 20g, white sugar 30g, yeast powder 5g, MgSO
40.5g, KH
2PO
40.5g, add water to 1L, pH6.5.
5, the production method of nutritious fungus feed addictive according to claim 2 is characterized in that: described first class seed pot liquid medium starting material weight proportion is: contain white sugar 30g, analysis for soybean powder 30g, MgSO in the 1000ml substratum
40.5g, KH
2PO
41g adds water after each component is mixed and mends to 1000ml, and is 6.5 with saturated NaOH (sodium hydroxide) adjust pH.
6, the production method of nutritious fungus feed addictive according to claim 2 is characterized in that: described secondary seed jar liquid medium starting material weight proportion is: contain white sugar 30g, analysis for soybean powder 30g, MgSO in the 1000ml substratum
40.5g, KH
2PO
41g adds water after each component is mixed and mends to 1000ml, and is 6.5 with saturated NaOH adjust pH.
7, the production method of nutritious fungus feed addictive according to claim 2 is characterized in that: described defoamer is a polyoxypropylene ethylene oxide glyceryl ether, and consumption is 0.03% of a nutrient solution total amount.
8, the production method of nutritious fungus feed addictive according to claim 2 is characterized in that: the solid substrate culture medium raw material weight proportion of described bacterial classification is: contain Semen Maydis grit 750g, bean cake powder 200g, wheat bran 50g, inorganic salt 2g in the 1000g substratum.
9, the production method of nutritious fungus feed addictive according to claim 2, it is characterized in that: the solid substrate culture process is a solid materials: water=5: 1, adopt tyndallization, during sterilising treatment solid materials is heated to 100 ℃, continue 20-45 minute, once a day, for three days on end, the last processing, but heat-up time proper extension; Be cooled to 24 ℃～25 ℃ inoculum sizes and insert liquid seeds with 8%; It is about 54% to mix final water ratio that the back guarantees material thoroughly, postvaccinal material is tiled in the thickness of 3cm puts into culturing room in the tray and cultivate; Temperature remain on 24 ℃～25 ℃ humidity remain on 95% cultivate 72h after, temperature is that 24 ℃～25 ℃ humidity drop to 85% and continue to cultivate discharging after 3～4 days; Oven dry or dry, pulverize, get product behind mistake 300 orders.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010115335A1 (en) * | 2009-04-08 | 2010-10-14 | Liu Weiping | Paecilomyces cicadae biological microbial agent, preparation method therefor and use thereof in controlling plant nematodes |
| CN101731461B (en) * | 2009-08-12 | 2012-07-04 | 南京南农高科兽药研究所有限公司 | Plant-based animal immune potentiator prepared by fermenting and developing radix astragali decoction dregs |
| CN102669426A (en) * | 2011-03-10 | 2012-09-19 | 上海泛亚生命科技有限公司 | Penicillium paecilomyces cicadae (miquel) samson fermentation product used as feed additive as well as preparation method and application thereof |
| CN106912293A (en) * | 2015-12-24 | 2017-07-04 | 浙江泛亚生物医药股份有限公司 | A kind of artificial culture method of cicada fungus |
| CN107259138A (en) * | 2016-04-06 | 2017-10-20 | 上海现代农业技术转移中心 | Application and pig feed of the cicada fungus as pig feed additive |
| CN108782022A (en) * | 2018-07-03 | 2018-11-13 | 淮北师范大学 | A method of it is primary raw material culture cicada fungus conidia powder to process waste residue using pomegranate |
| WO2019128487A1 (en) * | 2017-12-27 | 2019-07-04 | 浙江泛亚生物医药股份有限公司 | Isaria cicadae miquel feed for improving growth and immunity of poultry |
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2005
- 2005-01-04 CN CNB2005100069239A patent/CN1331413C/en active Active
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| WO2010115335A1 (en) * | 2009-04-08 | 2010-10-14 | Liu Weiping | Paecilomyces cicadae biological microbial agent, preparation method therefor and use thereof in controlling plant nematodes |
| CN101731461B (en) * | 2009-08-12 | 2012-07-04 | 南京南农高科兽药研究所有限公司 | Plant-based animal immune potentiator prepared by fermenting and developing radix astragali decoction dregs |
| CN102669426A (en) * | 2011-03-10 | 2012-09-19 | 上海泛亚生命科技有限公司 | Penicillium paecilomyces cicadae (miquel) samson fermentation product used as feed additive as well as preparation method and application thereof |
| CN102669426B (en) * | 2011-03-10 | 2014-05-07 | 浙江泛亚生物医药股份有限公司 | Penicillium paecilomyces cicadae (miquel) samson fermentation product used as feed additive as well as preparation method and application thereof |
| CN106912293A (en) * | 2015-12-24 | 2017-07-04 | 浙江泛亚生物医药股份有限公司 | A kind of artificial culture method of cicada fungus |
| CN106912293B (en) * | 2015-12-24 | 2020-08-25 | 浙江泛亚生物医药股份有限公司 | Artificial culture method of cordyceps sobolifera |
| CN107259138A (en) * | 2016-04-06 | 2017-10-20 | 上海现代农业技术转移中心 | Application and pig feed of the cicada fungus as pig feed additive |
| CN107259138B (en) * | 2016-04-06 | 2021-01-05 | 叶克全 | Application of cordyceps sobolifera as pig feed additive and pig feed |
| WO2019128487A1 (en) * | 2017-12-27 | 2019-07-04 | 浙江泛亚生物医药股份有限公司 | Isaria cicadae miquel feed for improving growth and immunity of poultry |
| CN111601513A (en) * | 2017-12-27 | 2020-08-28 | 浙江泛亚生物医药股份有限公司 | Cordyceps cicadae feed for improving growth and immunity of poultry |
| CN108782022A (en) * | 2018-07-03 | 2018-11-13 | 淮北师范大学 | A method of it is primary raw material culture cicada fungus conidia powder to process waste residue using pomegranate |
| CN108782022B (en) * | 2018-07-03 | 2020-10-20 | 淮北师范大学 | Method for culturing cordyceps sobolifera spore powder by using pomegranate processing waste residues as main raw materials |
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| Publication number | Publication date |
|---|---|
| CN1331413C (en) | 2007-08-15 |
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