CN1320065C - Red pigment and preparation method and application thereof - Google Patents
Red pigment and preparation method and application thereof Download PDFInfo
- Publication number
- CN1320065C CN1320065C CNB021467188A CN02146718A CN1320065C CN 1320065 C CN1320065 C CN 1320065C CN B021467188 A CNB021467188 A CN B021467188A CN 02146718 A CN02146718 A CN 02146718A CN 1320065 C CN1320065 C CN 1320065C
- Authority
- CN
- China
- Prior art keywords
- haematochrome
- carbon alcohol
- red pigment
- production method
- acid low
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000001054 red pigment Substances 0.000 title abstract description 30
- 238000002360 preparation method Methods 0.000 title abstract description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 43
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 17
- 239000011347 resin Substances 0.000 claims abstract description 17
- 229920005989 resin Polymers 0.000 claims abstract description 17
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 13
- 239000003463 adsorbent Substances 0.000 claims abstract description 12
- 238000004519 manufacturing process Methods 0.000 claims abstract description 11
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 5
- 239000002994 raw material Substances 0.000 claims abstract description 5
- 239000003814 drug Substances 0.000 claims abstract description 4
- 239000002537 cosmetic Substances 0.000 claims abstract description 3
- 235000013305 food Nutrition 0.000 claims abstract 2
- 239000002253 acid Substances 0.000 claims description 15
- 238000010521 absorption reaction Methods 0.000 claims description 4
- 235000021022 fresh fruits Nutrition 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
- 238000013467 fragmentation Methods 0.000 claims description 3
- 238000006062 fragmentation reaction Methods 0.000 claims description 3
- 238000007654 immersion Methods 0.000 claims 2
- 238000002386 leaching Methods 0.000 claims 1
- 235000008104 Prunus humilis Nutrition 0.000 abstract description 30
- 241000057271 Prunus humilis Species 0.000 abstract description 30
- 235000005706 Prunus prostrata Nutrition 0.000 abstract description 29
- 230000002378 acidificating effect Effects 0.000 abstract description 6
- 241000196324 Embryophyta Species 0.000 abstract description 5
- 238000002791 soaking Methods 0.000 abstract description 4
- 238000001179 sorption measurement Methods 0.000 abstract description 4
- 238000010276 construction Methods 0.000 abstract description 3
- 229940079593 drug Drugs 0.000 abstract description 2
- 239000000975 dye Substances 0.000 abstract description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract 2
- 238000001035 drying Methods 0.000 abstract 1
- 239000003480 eluent Substances 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 15
- 238000000605 extraction Methods 0.000 description 10
- 230000014759 maintenance of location Effects 0.000 description 10
- 239000000049 pigment Substances 0.000 description 10
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 8
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 6
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
- 230000002829 reductive effect Effects 0.000 description 6
- 239000004576 sand Substances 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 5
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 239000007864 aqueous solution Substances 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 239000000284 extract Substances 0.000 description 4
- 229910021645 metal ion Inorganic materials 0.000 description 4
- 239000011734 sodium Substances 0.000 description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- 239000000654 additive Substances 0.000 description 3
- 230000000996 additive effect Effects 0.000 description 3
- 239000011575 calcium Substances 0.000 description 3
- 238000003795 desorption Methods 0.000 description 3
- 238000005286 illumination Methods 0.000 description 3
- 229960002477 riboflavin Drugs 0.000 description 3
- 235000019192 riboflavin Nutrition 0.000 description 3
- 239000002151 riboflavin Substances 0.000 description 3
- 238000003828 vacuum filtration Methods 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- 239000011701 zinc Substances 0.000 description 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 150000003254 radicals Chemical class 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 229920006395 saturated elastomer Polymers 0.000 description 2
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 description 2
- 235000010234 sodium benzoate Nutrition 0.000 description 2
- 239000004299 sodium benzoate Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000167854 Bourreria succulenta Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 206010015150 Erythema Diseases 0.000 description 1
- FFEARJCKVFRZRR-UHFFFAOYSA-N L-Methionine Natural products CSCCC(N)C(O)=O FFEARJCKVFRZRR-UHFFFAOYSA-N 0.000 description 1
- 229930195722 L-methionine Natural products 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- XUYPXLNMDZIRQH-LURJTMIESA-N N-acetyl-L-methionine Chemical compound CSCC[C@@H](C(O)=O)NC(C)=O XUYPXLNMDZIRQH-LURJTMIESA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 238000000862 absorption spectrum Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000000274 adsorptive effect Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 229930014669 anthocyanidin Natural products 0.000 description 1
- 150000001452 anthocyanidin derivatives Chemical class 0.000 description 1
- 235000008758 anthocyanidins Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 235000019693 cherries Nutrition 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 235000011869 dried fruits Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 description 1
- DZGCGKFAPXFTNM-UHFFFAOYSA-N ethanol;hydron;chloride Chemical compound Cl.CCO DZGCGKFAPXFTNM-UHFFFAOYSA-N 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000000989 food dye Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- DKAGJZJALZXOOV-UHFFFAOYSA-N hydrate;hydrochloride Chemical compound O.Cl DKAGJZJALZXOOV-UHFFFAOYSA-N 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 229960004452 methionine Drugs 0.000 description 1
- JPXMTWWFLBLUCD-UHFFFAOYSA-N nitro blue tetrazolium(2+) Chemical compound COC1=CC(C=2C=C(OC)C(=CC=2)[N+]=2N(N=C(N=2)C=2C=CC=CC=2)C=2C=CC(=CC=2)[N+]([O-])=O)=CC=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=C([N+]([O-])=O)C=C1 JPXMTWWFLBLUCD-UHFFFAOYSA-N 0.000 description 1
- 239000012454 non-polar solvent Substances 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- BUKHSQBUKZIMLB-UHFFFAOYSA-L potassium;sodium;dichloride Chemical compound [Na+].[Cl-].[Cl-].[K+] BUKHSQBUKZIMLB-UHFFFAOYSA-L 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 235000020989 red meat Nutrition 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000012976 tarts Nutrition 0.000 description 1
- 238000001291 vacuum drying Methods 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Images
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a red pigment and a preparation method thereof, and aims to provide a novel red pigment and a preparation method thereof. The red pigment provided by the invention is extracted from fresh cerasus humilis fruits serving as raw materials. The production method of the haematochrome comprises the following steps: 1) crushing Prunus humilis Bunge fruit, and soaking in 30-99% acidic low carbon alcohol; 2) adsorbing the soaking solution with macroporous adsorbent resin; 3) eluting macroporous adsorption resin with 30-99% acidic low carbon alcohol; 4) concentrating and drying the eluent to obtain the haematochrome. The application of fresh cerasus humilis fruits perfectly combines the economic values of desertification land control and the development of the sandy plants, combines ecological construction and industrial development for the economic value of the discovery of the sandy plants to control desertification and have important practical significance and economic significance. The haematochrome of the invention can be widely applied to the food industry, cosmetics, medicines, health products, biological dyes and biological reagents.
Description
Technical field
The present invention relates to a kind of pigment and preparation method thereof and application, particularly a kind of haematochrome and preparation method thereof and application.
Background technology
Pigment is light industry, particularly the raw material of using always in the foodstuffs industry.Along with the raising of living standards of the people, the natural pigment that comes from plant or animal more and more is subjected to people's welcome, finds and extract the extensive concern that new natural pigment is subjected to the scientific worker.
Desertification is one of the serious environmental in the current whole world and social concern.The present desertification in China soil is very serious, and the desertification area of northern area is constantly expanded over nearly 50 years, has reached nearly 350,000 square kilometres, and is continuing expansion with annual 2460 square kilometres speed, and its rate of propagation has the trend that is quickening.Psammophyte has important effect in defending and controlling sand, excavate other economic worth of psammophyte, and ecological construction and industry development are combined, and with sand control cure sand, has important reality, society and economic implications.
Europe Lee (Cerasus humilis.BGE) is the psammophyte of rose department cherry genus.Former plant is a machaka, and root system is very flourishing, and root mass is huge, the about 0.5-1.5 rice of plant height, and the drought-enduring salt tolerant alkali that can resist cold, tool is good checks winds and fixes drifting sand, the soil conservation performance.Its fruit is contained nutritious, especially Vc, VB
2, V
EAnd the content of potassium, calcium, phosphorus, iron, zinc, selenium and Methionin all is higher than existing common fruit.Europe Lee's kernel and Gen Pi are as traditional Chinese medicine, and its nature,taste and action all has clearly record in Compendium of Material Medica and " legendary god of farming's book on Chinese herbal medicine ".
Summary of the invention
The purpose of this invention is to provide a kind of new haematochrome and preparation method thereof.
Haematochrome provided by the present invention is that raw material extracts with Europe Lee's fresh fruit.
The production method of this haematochrome may further comprise the steps:
1) with after the fragmentation of Europe Lee fruit, the acid low-carbon alcohol of 30-99% is soaked;
2) soak solution is through absorption with macroporous adsorbent resin;
3) the acid low-carbon alcohol wash-out of 30-99% macroporous adsorbent resin;
4) elutriant is concentrated, dry, obtains haematochrome.
The concentration of described acid low-carbon alcohol is benefit with 60-90%, preferably 80%.
In view of the safety problem of food dye, described acid low-carbon alcohol is chosen ethanol and is benefit.
Under the normal circumstances, the pH value of described acid low-carbon alcohol is 1.0-4.0; The temperature of soaking extraction is between 15-60 ℃; Soaking the time of extracting is 20 minutes-30 hours.
Flow velocity with acid low-carbon alcohol wash-out macroporous adsorbent resin is 2.0-3.0ml/min.
The method of production haematochrome of the present invention is not limited to above-mentioned technology, and the method for extracting pigment in the prior art all can be used to extract haematochrome of the present invention.
Pigment of the present invention is soluble in sour water, methyl alcohol, solvent that the ethanol isopolarity is bigger, is slightly soluble in ethyl acetate, is insoluble to acetone, chloroform, sherwood oil, the less or non-polar solvent of benzene isopolarity.Absorption spectrum 0.1%HCl methyl alcohol (530,0.854) in the different solvents, 0.1%HCl ethanol (530,0.962), 0.1%HCl50% ethanol water (524,1.288) 0.1%HCl water (508,1.376).
The Europe plum fruit that is used for production haematochrome of the present invention can be a fresh fruit, also can be the air dried fruit.Its source can be wild Europe Lee's a fruit, also can be Europe Lee's of domestication, cultivation fruit.Because Europe Lee's planting cost is low, and is bigger as ecological forest grass planting area, and the assurance of raw material aspect is provided for the suitability for industrialized production of haematochrome.Utilization to Europe Lee's fresh fruit, desertification land is administered with the economic worth of exploitation psammophyte obtained perfect the combination, the economic worth for excavating psammophyte combines ecological construction and industry development, with sand control cure sand, have important practical significance and economic implications.Haematochrome of the present invention is an all-natural product, all will be used widely in foodstuffs industry, cosmetic industry, biological dye, biological reagent.
Description of drawings
Fig. 1 is the influence curve of acidity (pH) to extraction rate
Fig. 2 is the influence curve of alcohol concn (pH) to extraction rate
Fig. 3 is the influence curve of time to extraction rate
Fig. 4 is the time adsorption curve of resin
Fig. 5 is the influence curve of the alcohol concn of wash-out to desorption efficiency
Embodiment
1, select excellent red skin red meat Europe Lee fruit, clean, stoning, dry, store stand-by;
2, take by weighing Europe Lee's pulp 2kg, 80% acidic ethanol soaked overnight;
3, soak solution concentrating under reduced pressure, centrifugal, AB-8 macroporous adsorbent resin 200g adsorbs concentrated solution;
4,80% acidic ethanol aqueous solution wash-out, flow velocity 2.5ml/min;
5, the elutriant vacuum concentration is air-dry, and vacuum-drying gets red-purple haematochrome powder 22g.
Embodiment 2, look valency are measured
The HCl aqueous solution constant volume that accurately takes by weighing embodiment 1 gained pigment powder 0.1g (accurately to 0.001g) usefulness pH=3 is made reference to 100ml with the HCl aqueous solution of pH=3, measures absorbance A with the 1cm cuvette down in the 509nm wavelength.
Look valency E (509nm)=Af/100*m=(0.825*200/0.1066)/100=15.47
A: absorbancy f: extension rate m: sample quality
Embodiment 3, different acidity (pH) are to the influence of extraction rate
In 6 beakers, respectively add 80% aqueous ethanolic solution 100ml, transfer to different pH values, add 25g Europe Lee's pulp soaked overnight again, extracting solution is carried out vacuum filtration, and different extracting solutions are transferred to pH=3.6 again and are surveyed its absorbancy down in 524nm, and the result as shown in Figure 1, X-coordinate is the pH value of extracting solution among the figure, ordinate zou is an absorbancy, the result from figure as can be seen, absorbancy weakens along with tart and descends.
Embodiment 4, alcohol concn are to the influence of extraction yield
Prepare the acidic ethanol aqueous solution 100ml (0.5%HCl of different concns respectively, pH=1.8), add 25g Europe Lee's pulp again, room temperature is placed 3h, extracting solution is carried out vacuum filtration, the filtrate dilution of getting equal volume is adjusted to alcohol concn for 1 times and is about 50%, surveys its absorbancy down in 520nm, and the result as shown in Figure 2, X-coordinate is the alcohol concn of extracting solution among the figure, ordinate zou is an absorbancy, the result from figure as can be seen, absorbancy rises along with the increase of alcohol concn.
Embodiment 5, temperature are to the influence of extraction yield
Extract the anthocyanidin experience according to forefathers, experiment is located at below 60 ℃.The 100ml80% aqueous ethanolic solution is transferred pH=3.0, puts into 30g Europe Lee's pulp respectively in 30 ℃, 40 ℃, 50 ℃, 60 ℃ thermostat water bath, constant temperature 2 hours, and vacuum filtration is measured absorbancy in 525nm, and the result is as shown in table 1
Table 1, temperature are to the influence of extraction yield
| Time | Absorbancy | |||
| 30 | 40 | 50 | 60℃ | |
| 0.2% 90min 0.02% after 60min 0.02% acid adjustment | 0.365 1.255 0.435 | 0.396 1.355 0.452 | 0.562 1.545 0.558 | 0.681 1.959 0.647 |
From the data of table 1 as can be seen: high temperature helps lixiviate, but prolongation in time has a declining tendency.Under the condition of room temperature, just reach balance in the time of 3 hours.
Embodiment 6, time are to the influence of extraction yield
Measure 400ml 80% aqueous ethanolic solution in the beaker of 800ml, transfer pH=3.0 with concentrated hydrochloric acid, put into 40g Europe Lee's pulp, every sampling in 5 minutes once, the centrifugal 5min of 1000/min surveys the absorbancy of supernatant liquor in the 524nm place, the result as shown in Figure 3, As time goes on, extraction yield rises gradually, tends towards stability during to 30 hours.
Embodiment 7, cerasus humilis red pigment stability experiment of the present invention
1, illumination is to the influence of pigment
Experiment condition and result are as shown in table 3:
Table 3, illumination are to the influence of cerasus humilis red pigment
| Time | The sunlight direct projection | Natural light | The dark place | ||||||
| 1.64 | 2.63 | 3.71 | 1.64 | 2.63 | 3.71 | 1.64 | 2.63 | 3.71 | |
| 0d 3d 6d 9d retention rate (%) | 1.097 0.988 0.928 0.807 74 | 0.936 0.866 0.818 0.736 79 | 0.403 0.314 0.287 0.255 63 | 0.514 0.438 0.409 0.383 84 | 0.514 0.448 0.418 0.392 86 | 0.514 0.435 0.402 0.377 83 | 0.513 0.440 0.412 0.396 87 | 0.513 0.453 0.429 0.405 89 | 0.513 0.438 0.409 0.393 87 |
From the data of table 3 as can be seen, illumination has certain influence for cerasus humilis red pigment.
2, temperature is to the influence of pigment
Get 5 experimental group and compare, the experiment condition and the result of 1-5 group are as shown in table 4:
Table 4, temperature are to the influence of cerasus humilis red pigment stability
| Temperature ℃ |
| 1 | 2 | 3 | 4 | 5 | |
| 30 retention rates (%), 60 retention rates (%), 90 retention rates (%) | 0.974 100 0.974 100 0.897 100 | 0.966 99.2 0.936 96.1 0.841 93.8 | 0.958 98.4 0.904 92.8 0.787 87.7 | 0.953 97.8 0.873 89.6 0.732 81.6 | 0.948 97.3 0.846 86.9 0.761 75.7 |
From the data of table 4 as can be seen, the cerasus humilis red pigment thermotolerance is relatively poor, and in the time of 90 ℃, heating 80min retention rate only is 84.8%.
3, pH is to the influence of cerasus humilis red pigment
Prepare certain density pigment solution, be transferred to different pH values with concentrated hydrochloric acid, heat down in 85 ℃, take a sample in 510nm every 30min and survey its absorbancy, condition and result are as shown in table 5:
Table 5, acidity are to the influence of cerasus humilis red pigment stability
| pH | The absorbance A at maximum wavelength place 520nm | Retention rate % | ||||
| 1.64 2.63 3.71 | 1.073 1.189 0.403 | 1.046 1.131 0.334 | 1.025 1.097 0.369 | 0.979 1.066 0.362 | 0.953 1.034 0.310 | 89 87 77 |
From the table the result as can be seen, the resistant to elevated temperatures ability of cerasus humilis red pigment is stronger under strong acidic condition.
4, metal ion is to the influence of cerasus humilis red pigment
Respectively with containing Na
+, K
+, Ca
2+, Mg
2+, Al
3+, Zn
2+, Sn
2+, Mn
2+, Sn
2+, Fe
3+The salt of metal ion is handled cerasus humilis red pigment, and treatment condition and result are as shown in table 6, the data from table as can be seen, metal ion is little to the influence of cerasus humilis red pigment stability.
Table 6, metal ion are to the influence of cerasus humilis red pigment stability
| NaCl | KCl | CaCl 2 | MgCl 2 | AlCl 3 | |||||
| Na +Concentration (mol/L) | The OD value | K +Concentration (mol/L) | The OD value | Ca 2+Concentration (mol/L) | The OD value | Mg 2+Concentration (mol/L) | The OD value | Al 3+Concentration (mol/L) | The |
| 0 1.6*10 -4 8.0*10 -4 16*10 -4 32*10 -4 | 0.612 0.740 0.766 0.791 0.815 | 0 1.6*10 -4 8.0*10 -4 16*10 -4 32*10 -4 | 0.612 0.525 0.516 0.508 0.504 | 0 4.0*10 -5 8.0*10 -5 16*10 -5 32*10 -5 | 0.558 0.560 0.557 0.558 0.557 | 0 1.6*10 -4 8.0*10 -4 16*10 -4 32*10 -4 | 0.964 0.963 0.945 0.921 0.888 | 0 0.4*10 -5 2.0*10 -5 4.0*10 -5 6.0*10 -5 | 0.558 0.551 0.546 0.542 0.526 |
| Retention rate | 1.332 | 0.824 | 0.998 | 0.921 | 0.943 | ||||
| ZnSO 4 | CuSO 4 | MnCl 2 | SnCl 2 | FeCl 3 | |||||
| Zn 2+Concentration (mol/L) | The OD value | Cu 2+Concentration (mol/L) | The OD value | Mn 2+Concentration (mol/L) | The OD value | Sn 2+Concentration (mol/L) | The OD value | Fe 3+Concentration (mol/L) | The |
| 0 0.4*10 -5 2.0*10 -5 4.0*10 -5 8.0*10 -5 | 0.964 0.933 0.909 0.895 0.826 | 0 0.4*10 -5 1.2*10 -5 2.4*10 -5 3.6*10 -5 | 0.551 0.541 0.530 0.517 0.511 | 0 4.0*10 -6 8.0*10 -6 16*10 -6 32*10 -6 | 0.706 0.730 0.851 0.874 0.889 | 0 0.4*10 -6 0.8*10 -6 1.6*10 -6 3.2*10 -6 | 0.558 0.541 0.232 0.164 0.134 | 0 2.0*10 -6 4.0*10 -6 8.0*10 -6 16*10 -6 | 0.612 0.599 0.534 0.371 0.289 |
| Retention rate | 0.857 | 0.927 | 1.259 | 0.240 | 0.472 | ||||
5, foodstuff additive are to the influence of cerasus humilis red pigment
Handle cerasus humilis red pigment with glucose, sucrose, citric acid, Vc, Sodium Benzoate respectively, treatment condition and result are as shown in table 7, and as can be seen from the table, foodstuff additive are little to the influence of cerasus humilis red pigment.
Table 7, common foodstuff additive are to the influence of cerasus humilis red pigment
| Glucose | Sucrose | Citric acid | V C | Sodium Benzoate |
| Concentration (mol/L) | The OD value | Concentration (mol/L) | The OD value | Concentration (mol/L) | The OD value | Concentration (mol/L) | The OD value | Concentration (mol/L) | The |
| 0 1.6*10 -4 8.0*10 -4 16*10 -4 32*10 -4 | 0.964 0.923 0.876 0.850 0.785 | 0 1.6*10 -4 8.0*10 -4 16*10 -4 32*10 -4 | 0.964 0.917 0.890 0.877 0.786 | 0 4.0*10 -5 8.0*10 -5 16*10 -5 32*10 -5 | 0.964 0.951 0.942 0.932 0.911 | 0 1.6*10 -4 8.0*10 -4 16*10 -4 32*10 -4 | 0.964 0.949 0.923 0.876 0.863 | 0 3.2*10 -4 16*10 -4 32*10 -4 64*10 -4 | 0.964 0.826 0.265 0.096 0.012 |
| Retention rate | 0.814 | 0.815 | 0.945 | 0.895 | 0.012 |
6, the oxygenant reductive agent is to the influence of cerasus humilis red pigment
Oxygenant H with different concns
2O
2With reductive agent Na
2SO
3Handle cerasus humilis red pigment, treatment condition and result are as shown in table 8, and as can be seen from the table, the oxygenant reductive agent all has stronger destruction to cerasus humilis red pigment.
Table 8, oxidation, reductive agent are to the influence of cerasus humilis red pigment
| Oxygenant H 2O 2 | Reductive agent Na 2SO 3 | ||
| Concentration (mol/L) | The OD value | Concentration (mol/L) | The |
| 0 3.2*10 -4 16*10 -4 32*10 -4 64*10 -4 | 0.964 0.907 0.585 0.321 0.192 | 0 1.6*10 -4 8.0*10 -4 16*10 -4 32*10 -4 | 0.964 0.644 0.262 0.163 0.104 |
| Retention rate | 0.199 | 0.108 | |
The mensuration of embodiment 8, the saturated adsorption time of macroporous adsorbent resin
Take by weighing the AB-8 macropore wet resin of 20g through activation treatment, 100ml pH=3.0 cerasus humilis red pigment extracting solution is adsorbed and constantly stirring, measure the absorbancy of supernatant liquor down in 510nm every 20min, draw adsorption curve, as shown in Figure 4, X-coordinate is time (branch) among the figure, and ordinate zou is an absorbancy, resin is tending towards saturated when as can be seen from the figure, adsorbing 40 minutes.Adsorptive capacity 500ml/60g wet resin.
Embodiment 9, elutriant alcohol concn are to the influence of desorption efficiency
With the identical absorption of Different concentrations of alcohol wash-out the macroporous adsorbent resin of haematochrome of the present invention, condition and result as shown in Figure 5, as can be seen from the figure, alcohol concn is that 80% o'clock desorption efficiency is the highest.
Improved with reference to the Beauchamp method, make the concentration of riboflavin (chemical pure) be
L-methionine(Met) (biochemical reagents) concentration is
Nitroblue tetrazolium (NBT, Shanghai chemical reagents corporation of Chinese Medicine group) concentration is
At pH is in 7.0 media environments, and with the fluorescent lamp irradiation of 2 25w, the different time is measured absorbancy in the 560nm place, and reaction mechanism is: riboflavin is because photochmeical reaction produces O
2, range estimation system color no change.When not adding antioxidant in system's solution, prolongation along with light application time, solution is by the faint yellow mazarine that gradually becomes, and do not see blue the generation when adding different antioxidant, system itself has riboflavin to have cerasus humilis red pigment that absorbing phenomenon is all arranged, and sees that from phenomenon cerasus humilis red pigment has very strong resistance of oxidation.
Table 9, removing free radical ability are measured table
| Light application time (min) | Blank | Cerasus humilis red pigment 0.01% | Cerasus humilis red pigment 0.015% | Cerasus humilis red pigment 0.02% | Tea-polyphenol 0.02% |
| 0 30 60 90 | 0.003 0.249 0.374 0.725 | 0.057 0.052 0.050 0.044 | 0.132 0.125 0.119 0.113 | 0.165 0.158 0.152 0.145 | 0.016 0.019 0.015 0.013 |
Claims (10)
1, a kind of haematochrome is to be raw material with Europe Lee's fresh fruit, obtains by following leaching process:
1) with after the fragmentation of Europe Lee fruit, soaks with the acid low-carbon alcohol of 30-99%;
2) soak solution is through absorption with macroporous adsorbent resin;
3) the acid low-carbon alcohol wash-out of 30-99% macroporous adsorbent resin;
4) elutriant is concentrated, dry, obtains haematochrome.
2, the production method of the described haematochrome of claim 1 may further comprise the steps:
1) with after the fragmentation of Europe Lee fruit, soaks with the acid low-carbon alcohol of 30-99%;
2) soak solution is through absorption with macroporous adsorbent resin;
3) the acid low-carbon alcohol wash-out of 30-99% macroporous adsorbent resin;
4) elutriant is concentrated, dry, obtains haematochrome.
3, the production method of haematochrome according to claim 2 is characterized in that: the concentration of described acid low-carbon alcohol is 60-90%.
4, the production method of haematochrome according to claim 3 is characterized in that: the concentration of described acid low-carbon alcohol is 80%.
5, according to the production method of claim 2 or 3 or 4 described haematochrome, it is characterized in that: described low-carbon alcohol is an ethanol.
6, the production method of haematochrome according to claim 2 is characterized in that: the pH value of described acid low-carbon alcohol is 1.0-4.0; The temperature that described immersion is extracted is between 15-60 ℃; The time that described immersion is extracted is 20 minutes-30 hours.
7, the production method of haematochrome according to claim 2 is characterized in that: the flow velocity of described acid low-carbon alcohol wash-out macroporous adsorbent resin is 2.0-3.0ml/min.
8, the haematochrome of claim 1 is in Application in Food Industry.
9, the application of the haematochrome of claim 1 in cosmetic industry, biological dye, biological reagent.
10, the application of the haematochrome of claim 1 in medicine, healthcare products are produced.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021467188A CN1320065C (en) | 2002-11-04 | 2002-11-04 | Red pigment and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021467188A CN1320065C (en) | 2002-11-04 | 2002-11-04 | Red pigment and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1498922A CN1498922A (en) | 2004-05-26 |
| CN1320065C true CN1320065C (en) | 2007-06-06 |
Family
ID=34232852
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB021467188A Expired - Fee Related CN1320065C (en) | 2002-11-04 | 2002-11-04 | Red pigment and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1320065C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102304296A (en) * | 2011-06-23 | 2012-01-04 | 张家港市鸿钻贸易有限公司 | Method for preparing dye by plum peel and application thereof |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101805528B (en) * | 2010-04-07 | 2013-05-08 | 北京市农林科学院 | Extraction and purification method of cerasus humilis red pigment |
| CN102604418B (en) * | 2012-01-19 | 2013-11-13 | 常州美胜生物材料有限公司 | Extraction, separation and dyeing application of natural dye |
| CN103551098A (en) * | 2013-11-01 | 2014-02-05 | 合肥工业大学 | Method for generating hydroxyl free radical through controlling visible light |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1040042A (en) * | 1988-08-05 | 1990-02-28 | 谢静波 | The extracting method of natural brown pigment |
| CN1055546A (en) * | 1990-04-07 | 1991-10-23 | 北京林业大学 | A kind of natural edible red pigment and production technique thereof |
| CN1304960A (en) * | 2000-12-12 | 2001-07-25 | 浙江大学 | Process for extracting natural red pigment from purple sweet potato with alcohol |
| WO2002017732A2 (en) * | 2000-08-31 | 2002-03-07 | Tempesta, Michael, S. | Efficient method for producing compositions enriched in anthocyanins |
-
2002
- 2002-11-04 CN CNB021467188A patent/CN1320065C/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1040042A (en) * | 1988-08-05 | 1990-02-28 | 谢静波 | The extracting method of natural brown pigment |
| CN1055546A (en) * | 1990-04-07 | 1991-10-23 | 北京林业大学 | A kind of natural edible red pigment and production technique thereof |
| WO2002017732A2 (en) * | 2000-08-31 | 2002-03-07 | Tempesta, Michael, S. | Efficient method for producing compositions enriched in anthocyanins |
| CN1304960A (en) * | 2000-12-12 | 2001-07-25 | 浙江大学 | Process for extracting natural red pigment from purple sweet potato with alcohol |
Non-Patent Citations (3)
| Title |
|---|
| 红肉李色素稳定性的研究 徐雅琴 于泽源 闫丽波，中国野生植物资源，第2期 1995 * |
| 红肉李色素稳定性的研究 徐雅琴 于泽源 闫丽波，中国野生植物资源，第2期 1995;鸡血李红色素稳定性的动力学研究 麻明友 肖卓炳 姚俊，吉首大学学报 自然科学版，第20卷第4期 1999 * |
| 鸡血李红色素稳定性的动力学研究 麻明友 肖卓炳 姚俊，吉首大学学报 自然科学版，第20卷第4期 1999 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102304296A (en) * | 2011-06-23 | 2012-01-04 | 张家港市鸿钻贸易有限公司 | Method for preparing dye by plum peel and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1498922A (en) | 2004-05-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Fernandez et al. | Effectiveness of Cupressus sempervirens cones as biosorbent for the removal of basic dyes from aqueous solutions in batch and dynamic modes | |
| Ramaiah et al. | Temporal variations in transparent exopolymer particles (TEP) associated with a diatom spring bloom in a subarctic ria in Japan | |
| Lutts et al. | Salinity influences biosorption of heavy metals by the roots of the halophyte plant species Kosteletzkya pentacarpos | |
| Kokubun et al. | Indoxyl derivatives in woad in relation to medieval indigo production | |
| CN101914075B (en) | Method for extracting fucoxanthin from brown algae | |
| Kumada et al. | The infra-red spectra of humic acids | |
| CN103464117A (en) | Preparation method of ethanediamine based porous dextrangel adsorbent | |
| Zelder et al. | Corrin-based chemosensors for the ASSURED detection of endogenous cyanide | |
| Shay et al. | Effect of low levels of calcium on exudation of sugars and sugar derivatives from intact peanut roots under axenic conditions | |
| CN1320065C (en) | Red pigment and preparation method and application thereof | |
| CN111167417A (en) | Modified bagasse, preparation method thereof and application of modified bagasse as adsorbent | |
| Shaikhiev et al. | Review of Peach (Prúnus pérsica) Shell Use to Remove Pollutants from Aquatic Environments | |
| CN110693030A (en) | Preparation method and application of macadimia nut green husk extract | |
| Treadwell Jr et al. | Photoconversion of riboflavin to lumichrome in plant tissues | |
| Velimirov | Sugar and lipid components in sea foam near kelp beds | |
| CN102875625B (en) | Extraction method of cyclic adenosine monophosphate (cAMP) with antiallergic activity from Chinese date | |
| CN105001284B (en) | A kind of purple spring wheat wheat bran anthocyanin extracting method | |
| CN113637092A (en) | Water shield polysaccharide and extraction method and application thereof | |
| Hu et al. | Enhanced removal of methylene blue from aqueous solution by pummelo peel pretreated with sodium hydroxide | |
| CN115844779B (en) | Total flavone and fruit acid part in citrus pulp, and preparation method and application thereof | |
| CN113996270A (en) | Preparation and application of modified medlar branch adsorbent | |
| CN107159148A (en) | A kind of method of divalent heavy metal ions in application hickory chick filament absorption waste water | |
| CN106889173A (en) | A kind of Shell of Water Chestnut chromocor extract with bacteriostatic activity and its preparation method and application | |
| CN112473181A (en) | Method for removing odor of biological extract | |
| CN112679452A (en) | Method for extracting fucoxanthin from kelp |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20070606 Termination date: 20091204 |