CN1364764A - Nanofiltering membrane process of purifying soybean oligosaccharide - Google Patents
Nanofiltering membrane process of purifying soybean oligosaccharide Download PDFInfo
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- CN1364764A CN1364764A CN 01101966 CN01101966A CN1364764A CN 1364764 A CN1364764 A CN 1364764A CN 01101966 CN01101966 CN 01101966 CN 01101966 A CN01101966 A CN 01101966A CN 1364764 A CN1364764 A CN 1364764A
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Abstract
In the nanofiltering membrane process, soybean whey water with soybean whey protein separated its treated with nanofiltering membrane to eliminate its salts, so that soybean oligosaccharide with high purity and density is obtained. The nanofiltering membrane has a trapped molecular weight range of 300-1000 melton, greater than that of reverse osmosis membrane and smaller than that of ultrafiltering membrane, so that it is used to desalt while trapping oligosaccharide with small molecular weight. Combining concentration and desalting results in high separation efficiency and higher stahyose and gossypose content.
Description
The present invention relates to a kind of carbohydrate purification techniques, especially relate to a kind of method of processing purification oligose the soybean whey water that produces from beans.
Soybean whey water is to produce in the process to the soyabean processing protein isolate, can produce in producing technologies such as soybean protein concentrate, soybean protein isolate and production bean curd, also can be obtained by hot water lixiviate skimmed soy beans dregs of beans.Soybean whey protein in the soybean whey water can be removed with ultrafiltration process or heating flocculence, and the composition situation of removing the later soybean whey water of whey-protein is as shown in table 1:
The composition (W/V) of whey water behind the table 1 separation soybean whey protein
| Whey-protein | Carbohydrate | Salt | Total solid |
| ??＜0.1 | ??0.5-1.0％ | ??0.2-1.0％ | ????0.7-2.1％ |
Carbohydrate wherein mainly is a soybean oligosaccharide, comprises stachyose, silk floss sugar and sucrose, and three's ratio is roughly 3: 1: 4.Stachyose and silk floss sugar have unique physiologically active, can be utilized by beneficial bacteria in the enteron aisle in the body, and they almost can be by all bacterial classification utilizations in the bifidus bacillus family, and the intestinal toxic bacterium almost can not utilize.Therefore, adopt effective ways purification oligose, have good prospect.
The method of traditional removal salt is the spent ion exchange resin desalination, and its shortcoming is a complicated operation, and resin needs frequent regeneration, consumes a large amount of chemical agents, simultaneously the regenerated liquid contaminate environment; The electrodialytic method desalination also is the extensive technology of utilization, and its power consumption is big, complicated operation, and exist the handling problem of the strong brine that contains high-enriched organics.
The purpose of this invention is to provide the desalination of a kind of using nanofiltration embrane method, thus the method for purifying soybean oligosaccharide.This method is held back carbohydrate and can see through salt simultaneously with specific film, collection concentrate with desalination be one, and have easy and simple to handle, save energy, separation efficiency height, the characteristics that environmental pollution is little.
The method of Nanofiltering membrane purifying soybean oligosaccharide of the present invention is handled the soybean whey water behind the separation soybean whey protein with nanofiltration membrane, slough salt wherein, improves the purity and the concentration of soybean oligosaccharide.
The further improvement of the present invention part is that described nanofiltration is the one-level nanofiltration, and the material of described film is polysulfones, polyethersulfone or aromatic polyamides, and molecular weight cut-off is 300-1000, operating pressure is 1.0-1.8MPa, temperature is 35-45 ℃, and in every operation three days, cleans nanofiltration membrane at least once.
Another improvements of the present invention are that described nanofiltration is the secondary nanofiltration, and its step is as follows:
(1) one-level nanofiltration, the material of described film is polysulfones, polyethersulfone or aromatic polyamides, molecular weight cut-off is 300-1000, in operating pressure is 1.0-1.8MPa, temperature is 35-45 ℃, the pH value is carried out the one-level nanofiltration under the condition of 5-8, and cleans film at least once in moving three days, cleans with the method that hot water injection and matting combine;
(2) to one-level nanofiltration concentrated solution thin up, amount of water is 1-6 a times of this concentrated solution volume;
(3) liquid glucose after the above-mentioned dilution is carried out the secondary nanofiltration, the material of described film is polysulfones, polyethersulfone or aromatic polyamides, molecular weight cut-off is 300-1000, operating pressure is 1.0-1.8MPa, temperature is 35-45 ℃, the pH value is 5-8, and cleans film at least once in moving three days, cleans with the method that hot water injection and matting combine.
Nanofiltration membrane described in the present invention can be a rolled film, also can be hollow-fibre membrane or tubular membrane.
The nanofiltration technique of one-level described in the present invention condition is: operating pressure 1.5Mpa, 40 ℃ of temperature, pH value are 6.0.
The nanofiltration technique of secondary described in the present invention condition is: operating pressure 1.4Mpa, 40 ℃ of temperature, pH value are 6.0.
The step of cleaning nanofiltration membrane described in the present invention is that elder generation uses the hot water injection, uses acidic solution such as HCI solution and basic solution such as NaOH solution again, cleans nanofiltration membrane respectively, makes film recover membrane flux fully.
The invention has the advantages that the nanofiltration membrane molecular weight cut-off generally pauses in the 300-1000 road, its molecular weight cut-off scope is bigger than reverse osmosis membrane, littler than ultra-filtration membrane, therefore slough salt when holding back the oligose of small molecular weight, collection concentrates and desalination is an one, has simplified operational condition, improved separation efficiency, and stachyose and raffinose content are greatly improved, save energy, and can reduce environmental pollution.
Other details of the present invention and characteristics can be cheer and bright by reading the embodiment that greater detail hereinafter describes.
The present invention can carry out the one-level nanofiltration according to actual needs or carry out the secondary nanofiltration, now is described as most preferred embodiment with the secondary nanofiltration.
Certain soybean protein isolate manufacturer produces 200 tons of soybean whey water every day, with ultrafiltration process removal soybean whey protein wherein, extracts oligose then from the soybean whey water of 200 tons of/day removal soybean whey proteins.Be introduced into one-level nanofiltration equipment, processing condition are: pressure 1.5Mpa, and 40 ℃ of temperature, the pH value is 6.0, obtaining solid concentration is that 6.5% concentrated solution is 20 tons; This concentrated solution is added the purified water dilution in 3: 1 ratios; Enter secondary nanofiltration equipment again, processing condition are: pressure 1.4Mpa, and 40 ℃ of temperature, the pH value is 6.0, obtaining solid concentration and be 5.85% concentrated liquid glucose is 20 tons; Then, gained is concentrated liquid glucose decolour and concentrating under reduced pressure, again this concentrated solution being carried out spraying drying, then can to get oligosaccharide contg (stachyose and silk floss sugar) be 1.17 tons/day of 45% oligomeric Icing Sugar.In continuous purification process, the every operation of nanofiltration membrane is cleaned once in three days at least, and purging method is earlier with 45 ℃ hot water injection, cleans nanofiltration membrane respectively with the hydrochloric acid soln of pH=3 and the NaOH solution of pH=11.5 again, makes film recover membrane flux fully; All use the rolling nanofiltration membrane of material as polyethersulfone in the firsts and seconds nanofiltration, its molecular weight cut-off is 300.
Claims (8)
1, a kind of method of Nanofiltering membrane purifying soybean oligosaccharide is handled the soybean whey water behind the separation soybean whey protein with nanofiltration membrane, slough salt wherein, improves the purity and the concentration of soybean oligosaccharide.
2, the method for Nanofiltering membrane purifying soybean oligosaccharide according to claim 1, it is characterized in that described nanofiltration is the one-level nanofiltration, the material of described film is polysulfones, polyethersulfone or aromatic polyamides, molecular weight cut-off is 300-1000, operating pressure is 1.0-1.8MPa, temperature is 35-45 ℃, and cleans nanofiltration membrane at least once in every operation in three days.
3, the method for Nanofiltering membrane purifying soybean oligosaccharide according to claim 2 is characterized in that described nanofiltration is the secondary nanofiltration:
(1) one-level nanofiltration, the material of described film is polysulfones, polyethersulfone or aromatic polyamides, molecular weight cut-off is 300-1000, in operating pressure is 1.0-1.8MPa, temperature is 35-45 ℃, the pH value is carried out the one-level nanofiltration under the condition of 5-8, and cleans once at least in moving three days, cleans with hot water injection and chemical process;
(2) to one-level nanofiltration concentrated solution thin up, amount of water is 1-6 a times of this concentrated solution volume;
(3) liquid glucose after the above-mentioned dilution is carried out the secondary nanofiltration, the material of its described film is polysulfones, polyethersulfone or aromatic polyamides, molecular weight cut-off is 300-1000, operating pressure is 1.0-1.8MPa, temperature is 35-45 ℃, the pH value is 5-8, and cleans once at least in moving three days, cleans with hot water injection and chemical process.
4, the method for Nanofiltering membrane purifying soybean oligosaccharide according to claim 2, the operating pressure that it is characterized in that the one-level nanofiltration is 1.5Mpa, and temperature is 40 ℃, and the pH value is 6.0.
5, the method for Nanofiltering membrane purifying soybean oligosaccharide according to claim 3, the operating pressure that it is characterized in that the one-level nanofiltration is 1.5Mpa, and temperature is 40 ℃, and the pH value is 6.0, and the operating pressure of secondary nanofiltration is 1.4Mpa, and temperature is 40 ℃, and the pH value is 6.0.
6,, it is characterized in that described nanofiltration membrane is rolled film, hollow-fibre membrane or tubular membrane according to the method for claim 1,2,3 or 4 described Nanofiltering membrane purifying soybean oligosaccharides.
7, according to the method for claim 2,3,4 or 5 described Nanofiltering membrane purifying soybean oligosaccharides, it is characterized in that the step of described cleaning nanofiltration membrane is, use the hot water injection earlier, clean respectively with acidic solution and basic solution again, make film recover membrane flux fully.
8, the method for Nanofiltering membrane purifying soybean oligosaccharide according to claim 6 is characterized in that the step of described cleaning nanofiltration membrane is, uses the hot water injection earlier, cleans respectively with acidic solution and basic solution again, makes film recover membrane flux fully.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 01101966 CN1131232C (en) | 2001-01-19 | 2001-01-19 | Nanofiltering membrane process of purifying soybean oligosaccharide |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01101966 CN1131232C (en) | 2001-01-19 | 2001-01-19 | Nanofiltering membrane process of purifying soybean oligosaccharide |
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| CN1364764A true CN1364764A (en) | 2002-08-21 |
| CN1131232C CN1131232C (en) | 2003-12-17 |
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| CN 01101966 Expired - Fee Related CN1131232C (en) | 2001-01-19 | 2001-01-19 | Nanofiltering membrane process of purifying soybean oligosaccharide |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101538291B (en) * | 2009-04-22 | 2011-12-21 | 南京凯通粮食生化研究设计有限公司 | Method for using a simulated movable bed to separate and prepare high-content soybean oligosaccharide |
| CN102423637A (en) * | 2011-08-03 | 2012-04-25 | 上虞帝瑞云涛化工有限公司 | Membrane separation system and purification method for purifying camphor sulfonic acid liquid |
| CN104530143A (en) * | 2014-12-31 | 2015-04-22 | 东北农业大学 | Method for preparing soybean whey oligosaccharide through coupling of heating, flocculating, air floating and ultra-filtering |
| CN107698628A (en) * | 2017-09-28 | 2018-02-16 | 开平健之源保健食品有限公司 | A kind of method that refined stachyose is purified using membrane separation technique |
| CN108031289A (en) * | 2017-12-06 | 2018-05-15 | 四川美富特水务有限责任公司 | The cleaning method of sucrose liquid film concentration systems and the film concentration systems |
| CN116675665A (en) * | 2023-04-21 | 2023-09-01 | 长春大学 | Extraction method of soybean isoflavone, soyasaponin, soyastachyose and raffinose without participation of organic chemical solvent |
-
2001
- 2001-01-19 CN CN 01101966 patent/CN1131232C/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101538291B (en) * | 2009-04-22 | 2011-12-21 | 南京凯通粮食生化研究设计有限公司 | Method for using a simulated movable bed to separate and prepare high-content soybean oligosaccharide |
| CN102423637A (en) * | 2011-08-03 | 2012-04-25 | 上虞帝瑞云涛化工有限公司 | Membrane separation system and purification method for purifying camphor sulfonic acid liquid |
| CN102423637B (en) * | 2011-08-03 | 2013-09-25 | 浙江云涛生物技术股份有限公司 | Membrane separation system and purification method for purifying camphorsulfonic acid solution |
| CN104530143A (en) * | 2014-12-31 | 2015-04-22 | 东北农业大学 | Method for preparing soybean whey oligosaccharide through coupling of heating, flocculating, air floating and ultra-filtering |
| CN107698628A (en) * | 2017-09-28 | 2018-02-16 | 开平健之源保健食品有限公司 | A kind of method that refined stachyose is purified using membrane separation technique |
| CN107698628B (en) * | 2017-09-28 | 2020-01-31 | 开平健之源保健食品有限公司 | method for purifying and refining stachyose by membrane separation technology |
| CN108031289A (en) * | 2017-12-06 | 2018-05-15 | 四川美富特水务有限责任公司 | The cleaning method of sucrose liquid film concentration systems and the film concentration systems |
| CN116675665A (en) * | 2023-04-21 | 2023-09-01 | 长春大学 | Extraction method of soybean isoflavone, soyasaponin, soyastachyose and raffinose without participation of organic chemical solvent |
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| Publication number | Publication date |
|---|---|
| CN1131232C (en) | 2003-12-17 |
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