CN1348097A - Fast quantitative urine iodine detecting reagent kit and urine iodine detecting method - Google Patents
Fast quantitative urine iodine detecting reagent kit and urine iodine detecting method Download PDFInfo
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- CN1348097A CN1348097A CN 01133682 CN01133682A CN1348097A CN 1348097 A CN1348097 A CN 1348097A CN 01133682 CN01133682 CN 01133682 CN 01133682 A CN01133682 A CN 01133682A CN 1348097 A CN1348097 A CN 1348097A
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Abstract
The urine iodine detecting reagent kit consists of counteracting liquid, analysis agent, neutralizing agent, reductant, indication agent, oxidant and serial iodine solution. In detection, the counteracting liquid comprising acyl oxychloride, sodium hypochlorite and chlorine dioxide in certain proportion is first used to counteract urine sample to decompose the urine matter to interfere iodine detection; the analysis agent and the neutralizing agent are utilized to decompose residual the counteracting liquid and regulate acidity; and the iodine catalytic reductant and oxidant are finally utilized to react and the indication agent is utilized to indicate reaction end point with the reaction time being recorded with one stopwatch. The urine iodine content is calculated based on the linear relationship between the logarithmic reaction time and the logarithmic iodine content. The present invention has the features of being fast, accurate and sensitive.
Description
Technical field
The invention belongs to the detectable class, belong to detectable and the detection method of measuring content of iodine in the urine particularly.
Background technology
Urine iodide level is an important indicator of estimating human body iodine nutrition condition.Can diagnose whether iodine deficiency of human body by detecting the urine content of iodine.
Content of iodine is very little in the urine, has only for 1,000,000/zero point several; The urine complicated component, the material that disturbs iodine to measure is a lot.Therefore, measure urine iodine and generally adopt eliminating interfering material earlier, utilize the character of several chemical reactions of catalysis of iodine then, indirect determination urine iodine.
Merck KGaA company proposed urine iodine half-quantitative detection kit in 1998, and this kit adopts the resin filter urine sample, and the organism that disturbs iodine to measure is adsorbed on the resin, got filtrate mensuration.Assay method adopts the reaction of catalysis of iodine tetramethyl biphenyl diamines and hydrogen peroxide, according to hydrogen peroxide the tetramethyl biphenyl diamines is oxidized to the tetramethyl biphenyl diquinone and manifests yellow, relatively more yellow depth degree comes sxemiquantitative to judge that urine iodine contains the iodine scope, detect to be divided into＜100 μ g/L, 100-300 μ g/L and＞300 μ g/L third gear.
Summary of the invention
The objective of the invention is according to above-mentioned present situation condition, aim to provide a kind of fast quantitative urine iodine detecting reagent kit and urine iodine detecting method that can fast, accurately detect content of iodine in the urine.
The implementation of the object of the invention is, fast quantitative urine iodine detecting reagent kit, and kit is become to be grouped into by following main agents,
(1) digestion solution: form or wherein single component is formed by peroxidating acyl chlorides, sodium hypochlorite, chlorine dioxide are compound, its total available chlorine content is (with Cl
2Meter) at 0.3～1.2mol./L,
(2) resolve agent, be urea solution, its concentration is 0.1～1mol/L in urea,
(3) neutralizing agent: sulfuric acid, hydrochloric acid or phosphoric acid solution, its acidity is counted 1～10mol/L with pH,
(4) reductive agent: be dissolved in the arsenic chloride solution that is prepared in the acid solution by arsenic trioxide, its content is 1 * 10 in arsenic trichloride
-3～0.5mol/L,
(5) indicator: Ferroin (ferrous spirit) solution, its concentration is 5.0 * 10 in Ferroin
-3～1.0 * 10
-2Mol/L,
(6) oxygenant: Cericammoniumsulfate or cerous sulfate solution, content are 5 * 10
-4～5 * 10
-2Mol/L.
Fast quantitative urine iodine detecting reagent kit detects the method for urine iodine, and concrete steps are:
(1) clears up the serial iodine standard solution that accurately pipettes 50,100,200,300 and 400 μ g/L and each 0.1-1ml of urine sample in test tube, respectively add the 0.2-2ml digestion solution, room temperature leaves standstill after 5 minutes violent jolting test tube repeatedly, and the gas that causes reaction to produce (is mainly N
2, O
2With a small amount of Cl
2) drain till, clearing up overall process needs 10-15 minute approximately;
(2) resolve every pipe and add 0.2-2ml ml and resolve agent, room temperature leaves standstill that violent jolting test tube repeatedly drains the gas that reaction produces after 2 minutes, and overall process must not be less than 10 minutes;
(3) the every pipe of neutralization adds the 0.2-2ml neutralizing agent, and violent jolting test tube repeatedly drains the gas that reaction produces, and overall process must not be less than 10 minutes;
(4) sample handled well was placed 10 minutes at the mensuration place, treat temperature balance after every pipe add the 0.2-2ml reductive agent, shake up;
(5) measure every pipe and add the 0.2-2ml indicator, shake up, a selected pipe, add the 0.2-2ml oxygenant fast with sample injector on the other hand, another hand starts manual time-keeping simultaneously, and takes out this test tube immediately and shake up, and this moment, test solution became blueness, carrying out along with reaction, test solution will become bluish violet from blueness, become redness at last, stop stopwatch when test solution becomes bluish violet and just red (catastrophe point) occurred, recording reacting time (second)
Repeat above operation, measure and write down all the other sample hose reaction time;
(6) calculating of urine content of iodine
Adopt the linear regression equation method to calculate the urine content of iodine, because of logarithm value lnC or the lgC of the logarithm value lnS of reaction time S (second) or lgS and iodine concentration C (μ g/L) linear, obtain the regression equation of typical curve, lnC=a+blnS or lgC=a+blgS by two recurrence statistical methods.A wherein, b is respectively the intercept and the slope of curve, is constant, tries to achieve by returning statistical method, and it is different constants under different temperatures, and the regression equation with reaction time (second) the substitution typical curve of sample hose calculates sample content of iodine (μ g/L).
The present invention with digestion solution (peroxidating acyl chlorides, sodium hypochlorite, chlorine dioxide or potpourri wherein) in vitro decompose urine in organism get rid of interfering material; Pass through to resolve the digestion solution of agent decomposing excessive again and regulate acidity; Reconcile acidity by neutralizing agent, and further decompose remaining digestion solution; By catalysis of iodine arsenious acid and cerous sulfate reaction, use indicator (Ferroin) Indicator Reaction terminal point then, the record reaction is from beginning to the needed time of terminal point (second).
The present invention in detection, the first pre-treatment process that iodine standard solution, urine sample are cleared up, resolve, neutralized, the suggestion of these processes is carried out in outdoor ventilation place or fuming cupboard, to avoid the suction of irritative gas.The sample handled well was placed 10 minutes at the mensuration place, treat temperature balance after, every pipe adds the 0.2-2ml reductive agent, shakes up.
During mensuration, every pipe adds the 0.2-2ml indicator, shakes up.Because of the easy passivation of indicator overlong time in solution, once the pipe number of Jia Ruing too much, is not advisable with 20 pipes.Be ready to stopwatch, minute book and a blank sheet of paper, below carry out the single tube operation.A selected pipe adds the 0.2-2ml oxygenant fast with sample injector on the other hand, and another hand starts manual time-keeping simultaneously, and takes out this test tube immediately and shake up, and test solution becomes blueness.With the blank sheet of paper is background, observes change in color in vitro.Along with the carrying out of reaction, test solution will become bluish violet from blueness, become redness at last.When becoming bluish violet and just red (catastrophe point) occurred, test solution stops stopwatch, recording reacting time (second).Catastrophe point normal root really needs only consistent according to operator's observation habit.
The calculating of urine content of iodine adopts the linear regression equation method to calculate the urine content of iodine.Because of logarithm value lnC or the lgC of the numerical value lnS of reaction time S (second) or lgS and iodine concentration C (μ g/L) linear, with the logarithm of the concentration of standard solution logarithm input function counter in corresponding its reaction time, obtain the regression equation of typical curve, lnC=a+blnS or lgC=a+blgS by two recurrence statistical methods.A, b are respectively the intercept and the slopes of curve in the formula, are different constants under different temperatures.With the regression equation of reaction time (second) the substitution typical curve of sample hose, calculation sample content of iodine (μ g/L).The sensitivity of this kit is 5 μ g/l.
Testing process of the present invention can be simplified, and resolving agent and neutralizing agent can selectivity add, and promptly iodine standard solution, urine sample add earlier and resolve agent or neutralizing agent after clearing up, and add reductive agent again and reduce.Neutralizing agent, reductive agent, indicator be three or wherein the two mixes the back and adds in advance, neutralizes simultaneously, reduces, indication process or two processes wherein.
Embodiment
Below enumerate embodiments of the invention:
Reagent composition and content
Digestion solution: available chlorine content is 0.6mol/l; Resolve agent: urea content is 0.25mol/l; Neutralizing agent: sulfuric acid concentration is 2.5mol/L; Reductive agent: arsenic trioxide content is 6.0 * 10
-2Mol/L; Indicator: Ferroin content is 3.6 * 10
-2Mol/L; Oxygenant: the content of Cericammoniumsulfate is 7.0 * 10
-3Mol/L.
Testing result
Detect by detection method of the present invention.Wherein, standard solution and urine sample are respectively got 0.2ml, and the consumption of other 6 kinds of reagent of every pipe is 0.5ml, and it is as follows to record one group of data under 17 ℃ of room temperatures:
Standard and sample (μ g/L) 50 100 200 300 400 samples 1 sample 2
Reaction time (S) case 78.03 50.91 31.43 25.24 20. 45.96 32.32
The regression equation of trying to achieve typical curve with function calculator is:
lnc＝10.759-1.571lns
With sample 1 and sample 2 reaction time substitution regression equation, the content of iodine that draws sample l is 115.1 μ g/L, and the content of iodine of sample 2 is 200.1 μ g/L.
Claims (4)
1, fast quantitative urine iodine detecting reagent kit is characterized in that kit is become to be grouped into by following main agents,
(1) digestion solution: form or wherein single component is formed by peroxidating acyl chlorides, sodium hypochlorite, chlorine dioxide are compound, its total available chlorine content is (with Cl
2Meter) at 0.3～1.2mol./L,
(2) resolve agent, be urea solution, its concentration is 0.1～1mol/L in urea,
(3) neutralizing agent: sulfuric acid, hydrochloric acid or phosphoric acid solution, its acidity is counted 1～10mol/L with pH,
(4) reductive agent: be dissolved in the arsenic chloride solution that is prepared in the acid solution by arsenic trioxide, its content counts 1 * 10 with arsenic trichloride
-3～0.5mol/L,
(5) indicator: Ferroin (ferrous spirit) solution, its concentration is 5.0 * 10 in Ferroin
-3～1.0 * 10
-2Mol/L,
(6) oxygenant: Cericammoniumsulfate or cerous sulfate solution, content are 5 * 10
-4～5 * 10
-2Mol/L.
2, fast quantitative urine iodine detecting reagent kit detects the method for urine iodine, it is characterized in that concrete steps are:
(1) clears up the serial iodine standard solution that accurately pipettes 50,100,200,300 and 400 μ g/L and each 0.1-1ml of urine sample in test tube, respectively add the 0.2-2ml digestion solution, room temperature leaves standstill after 5 minutes violent jolting test tube repeatedly, and the gas that causes reaction to produce (is mainly N
2, O
2With a small amount of Cl
2) drain till, clearing up overall process needs 10-15 minute approximately,
(2) resolve every pipe and add 0.2-2ml ml and resolve agent, room temperature leaves standstill that violent jolting test tube repeatedly drains the gas that reaction produces after 2 minutes, and overall process must not be less than 10 minutes,
(3) the every pipe of neutralization adds the 0.2-2ml neutralizing agent, and violent jolting test tube repeatedly drains the gas that reaction produces, and overall process must not be less than 10 minutes
(4) sample handled well was placed 10 minutes at the mensuration place, treat temperature balance after every pipe add the 0.2-2ml reductive agent, shake up,
(5) measure every pipe and add the 0.2-2ml indicator, shake up, a selected pipe, add the 0.2-2ml oxygenant fast with sample injector on the other hand, another hand starts manual time-keeping simultaneously, and takes out this test tube immediately and shake up, and this moment, test solution became blueness, carrying out along with reaction, test solution will become bluish violet from blueness, become redness at last, stop stopwatch when test solution becomes bluish violet and just red (catastrophe point) occurred, recording reacting time (second)
Repeat above operation, the reaction time of measuring and writing down all the other sample hoses,
(6) calculating of urine content of iodine
Adopt the linear regression equation method to calculate the urine content of iodine, because of logarithm value lnC or the lgC of the logarithm value lnS of reaction time S (second) or lgS and iodine concentration C (μ g/L) linear, obtain the regression equation of typical curve, lnC=a+blnS or lgC=a+blgS by two recurrence statistical methods.A wherein, b is respectively the intercept and the slope of curve, is constant, tries to achieve by returning statistical method, and it is different constants under different temperatures, and the regression equation with reaction time (second) the substitution typical curve of sample hose calculates sample content of iodine (μ g/L).
3, method according to claim 2 is characterized in that iodine standard solution, urine sample after clearing up, and adds to resolve agent or neutralizing agent, adds reductive agent again and reduces.
4, method according to claim 2, neutralizing agent, reductive agent, indicator be three or wherein the two mixes the back and adds in advance, neutralizes simultaneously, reduces, indication process or two processes wherein.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB01133682XA CN1170147C (en) | 2001-11-16 | 2001-11-16 | Fast quantitative urine iodine detecting reagent kit and urine iodine detecting method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB01133682XA CN1170147C (en) | 2001-11-16 | 2001-11-16 | Fast quantitative urine iodine detecting reagent kit and urine iodine detecting method |
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| Publication Number | Publication Date |
|---|---|
| CN1348097A true CN1348097A (en) | 2002-05-08 |
| CN1170147C CN1170147C (en) | 2004-10-06 |
Family
ID=4672016
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB01133682XA Expired - Lifetime CN1170147C (en) | 2001-11-16 | 2001-11-16 | Fast quantitative urine iodine detecting reagent kit and urine iodine detecting method |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1320351C (en) * | 2004-09-07 | 2007-06-06 | 上海大学 | Method for measuring casein iodide in fodder |
| CN102495203A (en) * | 2011-11-10 | 2012-06-13 | 武汉众生生化技术有限公司 | Reagent used for detecting urinary iodine by biochemistry analyzer and urinary iodine detecting method |
| CN102636483A (en) * | 2012-05-03 | 2012-08-15 | 李又兰 | Method and device for detecting urine iodine concentration |
| CN105004719A (en) * | 2015-07-21 | 2015-10-28 | 杨先锋 | Urine iodine determination kit and applications thereof |
| CN108776134A (en) * | 2018-08-14 | 2018-11-09 | 厦门市疾病预防控制中心（厦门市卫生检测检验中心） | One kind being based on the iodide ion detection reagent and method of catalysis of iodine hydrazine-[oxidant-Ferroin reagents] system |
| CN109540877A (en) * | 2017-09-22 | 2019-03-29 | 王九宏 | Urinate the reagent and detection method that above microplate reader detects after iodine and saliva iodine room temperature pre-treatment |
| CN109959656A (en) * | 2019-04-10 | 2019-07-02 | 湖北生命寓言智能科技有限公司 | A kind of iodine concentration automatic testing method, equipment and system |
| CN110320207A (en) * | 2019-07-23 | 2019-10-11 | 黄艳丽 | A kind of iodine detection reagent and detection method |
| CN110501966A (en) * | 2019-08-19 | 2019-11-26 | 中国石油天然气集团有限公司 | The control method and device of oil-based drill cuttings thermal desorption process oil content and residence time |
| CN110567948A (en) * | 2019-09-10 | 2019-12-13 | 张鹏 | Iodide ion detection kit |
| CN112082986A (en) * | 2020-09-17 | 2020-12-15 | 湖北文理学院 | Urine iodine detection method |
| CN112945943A (en) * | 2019-12-11 | 2021-06-11 | 文逢乐 | Iodine element detection kit and iodine element detection method |
| CN113358637A (en) * | 2021-05-11 | 2021-09-07 | 棒糖科技(杭州)股份有限公司 | Method for interpreting semi-quantitative test paper result through stabilization time |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101788495B (en) * | 2010-01-29 | 2011-10-26 | 白银有色集团股份有限公司 | Method for measuring arsenic by iodine solution absorption method |
-
2001
- 2001-11-16 CN CNB01133682XA patent/CN1170147C/en not_active Expired - Lifetime
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1320351C (en) * | 2004-09-07 | 2007-06-06 | 上海大学 | Method for measuring casein iodide in fodder |
| CN102495203A (en) * | 2011-11-10 | 2012-06-13 | 武汉众生生化技术有限公司 | Reagent used for detecting urinary iodine by biochemistry analyzer and urinary iodine detecting method |
| CN102495203B (en) * | 2011-11-10 | 2014-01-22 | 武汉众生生化技术有限公司 | Reagent used for detecting urinary iodine by biochemistry analyzer and urinary iodine detecting method |
| CN102636483A (en) * | 2012-05-03 | 2012-08-15 | 李又兰 | Method and device for detecting urine iodine concentration |
| CN102636483B (en) * | 2012-05-03 | 2014-04-16 | 李又兰 | Method and device for detecting urine iodine concentration |
| CN105004719A (en) * | 2015-07-21 | 2015-10-28 | 杨先锋 | Urine iodine determination kit and applications thereof |
| CN109540877A (en) * | 2017-09-22 | 2019-03-29 | 王九宏 | Urinate the reagent and detection method that above microplate reader detects after iodine and saliva iodine room temperature pre-treatment |
| CN108776134A (en) * | 2018-08-14 | 2018-11-09 | 厦门市疾病预防控制中心（厦门市卫生检测检验中心） | One kind being based on the iodide ion detection reagent and method of catalysis of iodine hydrazine-[oxidant-Ferroin reagents] system |
| CN109959656A (en) * | 2019-04-10 | 2019-07-02 | 湖北生命寓言智能科技有限公司 | A kind of iodine concentration automatic testing method, equipment and system |
| CN110320207A (en) * | 2019-07-23 | 2019-10-11 | 黄艳丽 | A kind of iodine detection reagent and detection method |
| CN110501966A (en) * | 2019-08-19 | 2019-11-26 | 中国石油天然气集团有限公司 | The control method and device of oil-based drill cuttings thermal desorption process oil content and residence time |
| CN110567948A (en) * | 2019-09-10 | 2019-12-13 | 张鹏 | Iodide ion detection kit |
| CN112945943A (en) * | 2019-12-11 | 2021-06-11 | 文逢乐 | Iodine element detection kit and iodine element detection method |
| CN112082986A (en) * | 2020-09-17 | 2020-12-15 | 湖北文理学院 | Urine iodine detection method |
| CN113358637A (en) * | 2021-05-11 | 2021-09-07 | 棒糖科技(杭州)股份有限公司 | Method for interpreting semi-quantitative test paper result through stabilization time |
| CN113358637B (en) * | 2021-05-11 | 2024-02-20 | 棒糖科技(杭州)股份有限公司 | Method for realizing interpretation of semi-quantitative test paper result through stable time |
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