CN1274337C - Scorpion venom cral liquor - Google Patents
Scorpion venom cral liquor Download PDFInfo
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- CN1274337C CN1274337C CNB021159947A CN02115994A CN1274337C CN 1274337 C CN1274337 C CN 1274337C CN B021159947 A CNB021159947 A CN B021159947A CN 02115994 A CN02115994 A CN 02115994A CN 1274337 C CN1274337 C CN 1274337C
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Abstract
The present invention relates to a scorpion venom oral liquid which belongs to the technical field of medical products. The scorpion venom oral liquid is mainly prepared by the method that 200 to 300 grams of scorpion venom extracts of live scorpions are dissolved in 80 to 120 milliliters of water to form water solution, 35 to 45 grams of safflower, 50 to 60 grams of wolfberry fruits and 40 to 50 grams of Chinese dates are added into 900 to 1200 milliliters of edible white wine of 42 to 51 DEG to be soaked and percolated to obtain extract liquid, and the water solution and the extract liquid are mixed to obtain the scorpion venom oral liquid. The scorpion venom oral liquid also contains 30 to 35 grams of crystal sugar or 1 to 1.2 grams of glucoprotein. The scorpion venom extracts can be obtained through grinding and alcohol extraction of scorpions and can also be obtained by adopting an electrical stimulating method. The oral liquid has the advantages of no toxicity, high nutritive value and favorable therapeutic effect on obstruction of cardio-cerebral vascular circulation systems. Resources of live scorpions can be used periodically, so that the availability of precious and limited wild resources is increased.
Description
Technical field
The invention belongs to medical configuration product technical field, particularly a kind of scorpion venom cral liquor that is applicable to paralytic and cardiovascular and cerebrovascular circulation system disorders patient.
Background technology
According to the record of Chinese medicine dictionary, the Scorpio nature and flavor are salty flat, return the north yin channel of foot; Function is a wind-expelling pain-stopping, the detoxifcation of stimulating the menstrual flow.With the scorpion person of being used as medicine following three kinds of methods are arranged: (1) is flooded Scorpio and other Chinese medicines together, is filtered, and makes oral liquid.Because scorpion venom is different with pharmaceutically active ingredient leaching condition in other, the very difficult while is extracted scorpion venom and other Effective Components of Chinese Herb comprehensively.(2), pulverizing back compatibility other Chinese medicines clean, dry Scorpio are made capsule or tablet.Scorpion venom and other Chinese medicine active constituent contents are low in made capsule or the tablet, are difficult to reach satisfactory therapeutic effects in taking.(3) Scorpio and other Chinese medicines being decocted into decoction together takes.Because decoct the temperature height, this method makes the pharmacologically active ten of scorpion venom go seven or eight, has a strong impact on drug effect.And said method only can disposablely use precious Scorpio resource, has both wasted limited resources, has increased enterprise's cost of raw material again greatly.
Summary of the invention
The object of the invention is to provide the scorpion venom cral liquor that a kind of effective ingredient is comprehensive, result of use is good.
For reaching above-mentioned purpose, the present invention adopts following technical scheme: a kind of scorpion venom cral liquor mainly is dissolved in the aqueous solution that forms in the 80-120 ml water and (2) 35-45 gram Flos Carthami, 50-60 gram Fructus Lycii, 40-50 gram Fructus Jujubae by the full scorpion venom extract of living scorpion of (1) 200-300 gram and adds 42-51 ° of soaking in Chinese liquor percolation of 900-1200 milliliter gained extracting solution and mix.
Also contain 30-35 gram crystal sugar; Described Chinese liquor is 42-51 ° of edible fermented wine.
Also contain 1-1.2 gram protein sugar; Described Chinese liquor is 42-51 ° of edible fermented wine.
The scorpion venom extract method for making is: get the fresh and healthy scorpion that totally lives and added normal saline by weight 1: 1 and grind homogenate, by weight adding behind 95% edible ethanol heating in water bath backflow at 1: 0.7 1-3 hour, cold filtration rear filtrate heating in water bath, concentrate scorpion venom extract.
The scorpion venom extract method for making is: get fresh and healthy scorpion totally alive electricity irritation scorpion alive under voltage 6-10 volt, frequency 120Hz condition and gather scorpion venom, vacuum lyophilization gets dry product; With the dilution of the sodium phosphate buffer of concentration 0.01M, PH=7.4, under 0-4 ℃, 11000-15000 rev/min condition centrifugal 10-15 minute; The supernatant column chromatography separates, and gets 250-320nm wavelength component, and vacuum lyophilization gets scorpion venom extract.
Product of the present invention is applicable to middle-aged and elderly people, particularly apoplectic hemiplegia patient and cardiovascular and cerebrovascular circulation system disorders person.Instructions of taking is: the preceding (medicine) being taken before meal usefulness of early sleeping in ante cibum, evening, every day twice, each 10ml.The above person of body weight Kg adds 10ml every day.Take in back 30 minutes and do not take other beverages.Take and should note: (1) this product contains precipitation, shakes up before taking; (2) take the normal reaction of the dizzy genus of the little sense in back; (3) the ischemic Hypertensive Population should take the circumstances into consideration to subtract in right amount clothes blood pressure lowering class medicine; (4) the part hemiplegic patient pain occurs in taking, and is the logical again phenomenons of meridians, can strengthen dose in right amount; (5) anemia of pregnant woman and cerebral hemorrhage phase are prohibited clothes.
Among the present invention, comprise rich in amino acid in the Scorpio extract, its main component is hemolysin, invasin and anticoagulant and thrombolytic material, and the effect of dredge the meridian passage, haemolysis thrombolytic is arranged.With it is monarch, makes full use of its hemolysin dilute blood, reduces the viscosity blood circulation promoting; Utilize its plasminogen activating factors, the thrombosis that has formed in the lysed blood; And utilize its contained invasin-hyaluronidase, make functional component reach human tissue organ rapidly.Yet after the apoplexy, its blood is capable not smooth, so get the sweet warm nature medicine of Fructus Lycii, function nourishes blood, and it is prosperous to make blood fill arteries and veins, and the limb bones of the body are moistened foster.More with Fructus Jujubae, the crystal sugar medicine of fostering the spirit of nobility, QI invigorating can hemopoietic first, and second the prosperous blood that helps of gas is capable, complements each other, and shared is minister.Reuse Flos Carthami blood circulation promoting and blood stasis dispelling, it is capable freely to transport blood, makes all tonics capable and stagnant.
Show according to the hygiene inspection that product of the present invention is carried out: lead tolerance (in Pb)＜0.004mg/l, manganese (in Mn)≤0.177mg/l, cyanide does not detect, alcoholic strength (v/v) 43%, methanol≤0.015g/100ml, Alcohols,fusel≤0.037g/100ml, aminoacid 〉=161.63g/ml.According to " toxicological evaluation of food safety procedure and method " GB15193.3-94, GB15193.4-94, GB15193.5-94, GB15193.7-94, the toxicology check that the present invention is carried out shows: its mouse oral LD50=21500mg/Kg, the nontoxic level in true border.Oral liquid of the present invention is in three genetic toxicity tests such as mouse microkernel test, mouse sperm deformity test, Salmonella reversion test, and the result is all negative.According to GB/T1229290 and GB/T15400-94 threpsology's check that oral liquid of the present invention carries out is shown, contain in the oral liquid of the present invention: aspartic acid 〉=10.55mg/100g, threonine 〉=6.85mg/100g, serine 〉=9.09mg/100g, glutamic acid 〉=17.18mg/100g, glycine 〉=4.90mg/100g, alanine 〉=18.18mg/100g, cystine 〉=3.12mg/100g, valine 〉=12.70mg/100g, the methionine trace, isoleucine 〉=6.54mg/100g, leucine 〉=17.00mg/100g, tyrosine 〉=6.43mg/100g, phenylalanine 〉=6.80mg/100g, lysine 〉=8.94mg/100g, the histidine trace, arginine 〉=21.40mg/100g, proline 〉=6.00mg/100g, tryptophan 〉=5.94mg/100g, aminoacid summation 〉=161.63mg/100g.Utilize oral liquid of the present invention that 104 routine out-patients have been carried out clinical practice, among the 104 routine patients, male 47 people, women 57 people, the age is from 33 years old to 82 years old, being in the great majority more than 50 years old.The course of disease between 6 months to 15 years, half a year-one year 33 people wherein, 1-3 38 people, 3-5 24 people, 5-10 8 people, 15 years 1 people.Age-based meter, the right side of fifty 25 people, 51-60 year 36 people, 61-70 year 32 people, 71-82 year 11 people.Concrete diagnosis and efficacy assessment standard and observed content are as follows:
One, choice criteria
(1) diagnostic criteria
1, tcm diagnosis standard (with reference to " the apoplexy tcm diagnosis efficacy assessment standard " of All-China Association of Traditional Chinese Medicine's internal medicine revision in 1986)
(1) primary symptom: hemiplegia, crooked mouth and tongue, the mind unconsciousness, stiff tongue speech is stuttering or in silence, hemianesthesia.
(2) Acute onset.
(3) sick sending out has inducement more, and premonitory symptom is often arranged before not sending out.
(4) how send out the age well more than 40 years old.
Have primary symptom more than two, Acute onset can be determined diagnosis in conjunction with aspect characteristics such as tongue, arteries and veins, inducement, tendency, ages.
2, Chinese medical discrimination
(1) apoplex involving the channels and collaterals
Wind-phlegm congestion, numbness resistance venation card: hemiplegia, crooked mouth and tongue, the stiff tongue speech is stuttering or in silence, hemianesthesia is had a dizzy spell, dimly pale tongue, thin white fur of tongue or white greasy, stringy and rolling pulse.
The hot internal organs of expectorant is real, the paralysis upper harassing syndrome: hemiplegia, and crooked mouth and tongue, the stiff tongue speech is stuttering or in silence, hemianesthesia, abdominal distention, the dry stool constipation is had a dizzy spell, and coughs up phlegm or abundant expectoration dimly red tongue or dimness, yellow fur or yellow greasy, stringy and rolling pulse or hemiplegia side stringy and rolling pulse and big.
The gas syndrome of blood stasis that hisses: hemiplegia, crooked mouth and tongue, the stiff tongue speech is stuttering or in silence, hemianesthesia, the complexion HUANGBAI(sic), shortness of breath and fatigue, mouthful sialorrhea, from sweating, cardiopalmus loose stool, brothers' swelling, dimly pale tongue, thin white fur of tongue or white greasy, deep-thready pulse, slow or thin string carefully.
Stirring of wind due to deficiency of YIN card: hemiplegia, crooked mouth and tongue, the stiff tongue speech is stuttering or in silence, hemianesthesia, irritated insomnia, vertigo and tinnitus, feverish sensation in the palms and soles, deep red tongue or dark red, few tongue or no fur, thready and stringy pulse or thin stringy and rapid pulse.
3, apoplexy standard by stages
(1) acute stage: morbidity is in 2 weeks, and middle internal organs grow to 1 month most.
(2) convalescent period: fell ill for 2 thoughtful half a year.
(3) sequela stage: fall ill more than half a year.
(2) test case standard
1, includes the case standard in
The patients during acute stage that meets the apoplexy diagnostic criteria can be included the test case in.
2, get rid of the case standard
(1) transient ischemic attack.
(2) merge the atrial fibrillation person that causes the cerebral embolism through verifying by the cerebral tumor, cerebral trauma, cerebral parasitosis, dysbolismus, rheumatic heart disease, coronary heart disease and other heart disease.
(3) more than 70 years old, and gestation or feed women as scheduled is to this medicine allergy sufferers.
(4) be associated with serious primary disease such as liver, kidney, hemopoietic system and hormonal system, psychotic.
(5) all standards of including in that do not meet, not medication in accordance with regulations can't be judged that curative effect or data are not congruent to affect the treatment or safety judgement person.
Two, curative effect determinate standard
1, apoplexy point system
Adopt scoring method, mainly evaluate mind, language, motor function recovery degree.
(1) mind state: conscious 0 minute; Staring spells (drowsy, wake up after can and people's words) 1 minute; Mind mist (drowsiness, answering of exhaling is imprecise) 2 minutes; Coma 3 minutes; Muddle-headed (coma has concurrently simultaneously and takes off disease) 4 minutes.
(2) language performance: normal 0 minute; The general expression, name can not 1 minute; Form a complete sentence in a minute and expressed incomplete 2 minutes; Not talkative word, phrase 3 minutes; Language can not or substantially can not 4 minutes.
(3) limb shoulder joint: normal 0 minute; Normal but the muscular strength of last act differed from 1 fen; Last act is put down shoulder or is skipped over shoulder 2 minutes; Last act is less than takeing on 3 fens; Slightly swung 4 fens inactive or front and back.
(4) upper limb articulations digitorum manus: normal 0 minute; Action is effectively and muscular strength differed from 1 fen respectively for finger; Clench fist to stretch and refer to 2 fens; Count on one's fingers, can not hold fist, can not stretch 3 fens; Can not move 4 fens.
(5) lower limb hip joint: normal 0 minute; Raised more than 45 ° 1 fen; 45 ° of persons of less than 2 minutes; Swing energy translation 3 minutes; Inactive 4 minutes.
(6) lower limb toe joint: normal 0 minute; Stretch and bend freely but weak 1 minute of power; Stretch and bent incomplete 2 minutes; Slightly moving 3 minutes; Can not move 4 fens.
(7) comprehensive function: life can be taken care of oneself, open 0 minute; Live on one's own life, simple work and partial function is arranged incomplete 1 minute; Can walk partial self-help, auxiliary 2 minutes of the people that still needs; Can station power take a step, need the people to take care of at any time 3 fens; Liied in bed 4 fens.
2, imitate evaluation criteria
Full marks are 28 minutes before treating, and the starting point branch is the highest to be no more than 18 fens, and efficacy evaluation adopts the nimodipine method:
[integration before (integration before the treatment-treatment back integration) ÷ treatment] 100% represented with percent.
(1) is almost recovered: 〉=85%.(integration increases by 4 fens persons of less than)
(2) produce effects: 〉=50%.(integration increases above 4 fens persons)
(3) effective: 〉=20%.(integration increases above 10 fens persons)
(4) invalid:＜20%.(integration increases above 24 fens persons)
Three, conclusion
Treat 104 philtrums, male 47 people, women 57 people, the age is in the great majority more than 50 years old between 33-82 year, and the course of disease general orally takes effect in the moon in the period of half a year-15, and recovery from illness in three to five months or be almost recovered can allow the patient take care of oneself at least.104 routine patient treatments are shown, cure rate 52.88%, produce effects 20.19%, effective 20.11%, invalid 4.8%, total effective rate 95.2%.The course of disease and curative effect, age and curative effect see Table 1, table 2.
Table 1
| The course of disease | Number | Cure | Produce effects | Effectively | Invalid | Total effective rate |
| 0.5-1 year | 33 | 16 | 6 | 11 | ||
| 1-3 | 38 | 21 | 7 | 8 | 2 | |
| 3-5 | 24 | 14 | 4 | 3 | 3 | |
| 5-10 | 8 | 4 | 3 | 1 | ||
| 10-15 | 1 | 1 | ||||
| Percent | 52.88 | 20.19 | 22.11 | 4.8 | 95.2 |
Table 2
| Age | Number | Cure | Produce effects | Effectively | Invalid | Total effective rate |
| ＜50 years old | 25 | 19 | 3 | 3 | ||
| 51-60 year | 36 | 18 | 7 | 10 | 1 | |
| 61-70 year | 32 | 15 | 6 | 9 | 2 | |
| 71-82 year | 11 | 3 | 5 | 1 | 2 | |
| Percent | 52.88 | 20.19 | 22.11 | 4.8 | 95.2 |
The specific embodiment
Embodiment 1, a kind of scorpion venom cral liquor, by the full scorpion venom extract of living scorpion of (1) 200 gram be dissolved in the aqueous solution that forms in 80 milliliters of pure water and (2) 45 gram Flos Carthamis, 60 gram Fructus Lycii, 50 gram Fructus Jujubaes add 1200 milliliters 42 ° edible fermented wine soak percolation extracting solution mix.Flos Carthami, Fructus Lycii, Fructus Jujubae extracting solution method for making are: three kinds of compositions getting drying, cleaning are broken into fritter (about about 2cm), put in the percolation device and carry out percolation with edible fermented wine, collect percolate, and vacuum filtration gets percolate.Keep liquid level to be higher than percolation thing 1.5-2cm in the percolation process.The scorpion venom extract method for making is: get fresh and healthy scorpion totally alive and added normal saline grinding homogenate by weight 1: 1, heating in water bath refluxed 1 hour after adding 95% edible ethanol by weight at 1: 0.7, put cold filtration, three times repeatedly, the merging filtrate heating in water bath, concentrate the paste scorpion venom extract.
Embodiment 2, a kind of scorpion venom cral liquor comprise that the full scorpion venom extract of living scorpion of (1) 300 gram is dissolved in the aqueous solution that forms in 120 milliliters of pure water and adds 900 milliliters 46 ° edible fermented wine immersion percolation gained extracting solution with (2) 35 gram Flos Carthamis, 50 gram Fructus Lycii, 40 gram Fructus Jujubaes.The scorpion venom extract method for making is: get fresh and healthy scorpion totally alive and added normal saline grinding homogenate by weight 1: 1, heating in water bath refluxed 3 hours after adding 95% edible ethanol by weight at 1: 0.7, cold filtration rear filtrate heating in water bath, concentrate the paste scorpion venom extract.30 gram crystal sugar dissolve, mixing gets oral liquid of the present invention adding in (1), (2) mixing gained mixed liquor.
Embodiment 3, a kind of scorpion venom cral liquor comprise that the full scorpion venom extract of living scorpion of (1) 250 gram is dissolved in the aqueous solution that forms in 100 milliliters of pure water and adds 1100 milliliters 51 ° edible fermented wine immersion percolation gained extracting solution with (2) 40 gram Flos Carthamis, 55 gram Fructus Lycii, 45 gram Fructus Jujubaes.The scorpion venom extract method for making is: get fresh and healthy scorpion totally alive and added normal saline grinding homogenate by weight 1: 1, heating in water bath refluxed 2 hours after adding 95% edible ethanol by weight at 1: 0.7, cold filtration rear filtrate heating in water bath, concentrate the paste scorpion venom extract.1.1 gram protein sugar dissolve, mixing gets oral liquid of the present invention adding in (1), (2) mixing gained mixed liquor.
In embodiment 4, the present embodiment, the scorpion venom extract method for making is: get fresh and healthy scorpion totally alive electricity irritation under 6 volts of voltages, frequency 120Hz condition scorpion alive and gather scorpion venom, vacuum lyophilization gets dry product; With the dilution of the sodium phosphate buffer of concentration 0.01M, PH=7.4, under 4 ℃, 15000 rev/mins conditions centrifugal 10 minutes; The supernatant column chromatography separates, and gets 250-320nm wavelength component, and vacuum lyophilization gets scorpion venom extract.Other are with embodiment 1.
In embodiment 5, the present embodiment, the scorpion venom extract method for making is: get fresh and healthy scorpion totally alive electricity irritation under 8 volts of voltages, frequency 120Hz condition scorpion alive and gather scorpion venom, vacuum lyophilization gets dry product; With the dilution of the sodium phosphate buffer of concentration 0.01M, PH=7.4, under 1 ℃, 11000 rev/mins conditions centrifugal 15 minutes; The supernatant column chromatography separates, and gets 250-320nm wavelength component, and vacuum lyophilization gets scorpion venom extract.Other are with embodiment 2.
In embodiment 6, the present embodiment, the scorpion venom extract method for making is: get fresh and healthy scorpion totally alive electricity irritation under 10 volts of voltages, frequency 120Hz condition scorpion alive and gather scorpion venom, vacuum lyophilization gets dry product; With the dilution of the sodium phosphate buffer of concentration 0.01M, PH=7.4, under 2 ℃, 13000 rev/mins conditions centrifugal 13 minutes; The supernatant column chromatography separates, and gets 250-320nm wavelength component, and vacuum lyophilization gets scorpion venom extract.Other are with embodiment 3.
Claims (5)
1, a kind of scorpion venom cral liquor, the present invention is characterised in that main scorpion venom extract by 200-300 gram scorpion entirely alive is dissolved in the aqueous solution that forms in the 80-120 ml water and 35-45 gram Flos Carthami, 50-60 restrain Fructus Lycii and 42-51 ° of soaking in Chinese liquor percolation of 40-50 gram Fructus Jujubae adding 900-1200 milliliter gained extracting solution mixes.
2, oral liquid as claimed in claim 1 is characterized in that, also contains 30-35 gram crystal sugar; Described Chinese liquor is 42-51 ° of edible fermented wine.
3, oral liquid as claimed in claim 1 is characterized in that, also contains 1-1.2 gram protein sugar; Described Chinese liquor is 42-51 ° of edible fermented wine.
4, as claim 1,2 or 3 described oral liquids, it is characterized in that, the scorpion venom extract method for making is: get fresh and healthy scorpion totally alive and added normal saline grinding homogenate by weight 1: 1, by weight adding behind 95% edible ethanol heating in water bath backflow at 1: 0.7 1-3 hour, cold filtration rear filtrate heating in water bath, concentrate scorpion venom extract.
5, as claim 1,2 or 3 described oral liquids, it is characterized in that the scorpion venom extract method for making is: get fresh and healthy scorpion totally alive electricity irritation scorpion alive under voltage 6-10 volt, frequency 120Hz condition and gather scorpion venom, vacuum lyophilization gets dry product; With the dilution of the sodium phosphate buffer of concentration 0.01M, PH=7.4, under 0-4 ℃, 11000-15000 rev/min condition centrifugal 10-15 minute; The supernatant column chromatography separates, and gets 250-320nm wavelength component, and vacuum lyophilization gets scorpion venom extract.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021159947A CN1274337C (en) | 2002-06-18 | 2002-06-18 | Scorpion venom cral liquor |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021159947A CN1274337C (en) | 2002-06-18 | 2002-06-18 | Scorpion venom cral liquor |
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| CN1465352A CN1465352A (en) | 2004-01-07 |
| CN1274337C true CN1274337C (en) | 2006-09-13 |
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| CN106108009A (en) * | 2016-06-24 | 2016-11-16 | 周口师范学院 | Herba Cirsii extracting solution, health product containing Herba Cirsii active component and preparation method thereof |
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