CN1240136A - Medicine for promoting growth of vagina gram-positive bacillus and increasing vagina acidity and usage thereof - Google Patents

Abstract

The present invention relates to a medicine using glycogen as active component for directly promoting growth of vagina gram-positive bacillus and increasing vagina acidity, the application of glycogen in preparation of the above-mentioned medicine, and especially a method for directly promoting the growth of vagina gram-positive bacillus and increasing vagina acidity, and a method for curing attenuation of vagina acidity and its related diseases of vginitis, flora imbalance of vagina and bacterial vaginopathy.

Description

Directly promote the medicament and the purposes of growth of vagina gram-positive bacillus, enhancing vagina acidity

The present invention relates to a kind of is the direct promotion growth of vagina gram-positive bacillus of active component, the medicament of enhancing vagina acidity with the glycogen, relate to glycogen and directly promote growth of vagina gram-positive bacillus in preparation, strengthen the application in the medicament of vagina acidity, the method that also be particularly related to a kind of direct promotion growth of vagina gram-positive bacillus, strengthens vagina acidity weakens with the treatment vagina acidity and relevant vaginitis, the method for vaginal dysbacteriosis, particularly bacterial vaginosis.

Just often be acid in the vagina, pH scope 4.0-4.6, this is for keeping vaginal health to have crucial meaning.The vagina dominant microflora that acid therewith microhabitat adapts is the Gram-positive bacillus with the acid of producing, acidproof ability; These produce sour acid proof Gram-positive bacillus is lactobacillus (Lactobacillus) mostly, accidental bacillus bifidus, some bacillus cereus etc.; And gram negative bacilli, coccus etc. are relatively poor to sour toleration, thereby the amount in normal vagina less or seldom.When a variety of causes cause in the vagina positive bacillus minimizing or disappearance, when the vagina pH value raises, the growth that abnormal bacterials such as multiple gram negative bacilli, negative cocci and gram-positive cocci will occur increases, and forms vaginal dysbacteriosis.Unusually the gram negative bacilli that increases during vaginal dysbacteriosis, negative coccis etc. are harmful, wherein especially with bacterial vaginosis (Bacterial Vaginosis, BV) the most typical, its bacterial flora alternation is characterized as Gram-positive bacillus minimizing even disappearances such as ability of Lactobacillus in human vagina, vagina acidity weakens, pH value rising (pH＞4.6), and the Gardner Salmonella, bacteroid, negative coccis such as negative bacillus such as Mobiluncus and Wei Rong Shi coccus, and positive coccus such as streptococcus or the like abnormal bacterial raised growth increases, cause the leucorrhea stink, a series of symptom such as pruritus vulvae, and can cause fetal intrauterine growth retardation (IUGR) [3], premature labor (PTL), premature rupture of fetal membrane (PROM) [4], miscarriage and obstetrics infect, chorioamnionitis, puerperal endometritis, reproductive tract infection on the women such as hysterectomy postoperative vaginal wall cellulitis, urinary system infection or the like [1].The sickness rate of vaginal dysbacteriosis is very high, be women's common disease, bibliographical information is arranged, about female vagina more than 45% per vaginam dysbacteriosis causes [1], the sickness rate of U.S. collegegirl bacterial vaginosis is 4-15%[2], bring serious harm for female body Health and Living quality.

But at present to vagina gram-positive bacillus reduces, vagina acidity weakens relevant vaginal dysbacteriosis, vaginitis, especially bacterial vaginosis control difficulty relatively.The Therapeutic Method of primary study and employing mainly contains both at home and abroad at present:

1) antibacterials: promptly suppress the abnormal bacterials such as gram negative bacilli increase with antibacterials.Using maximum medicines is clindamycin and metronidazole [5,6], antibacterial to supernormal growth in the vagina has rapid and sure sterilization, fungistatic effect, but it is not so good that Gram-positive bacillus (lactobacillus) recovers, and the pH value of vagina also is difficult to reduce to normal range.Domestic research document about vaginal dysbacteriosis and BV aspect is still rare, but mainly also is to treat with various antibacterials and some Chinese herbal and crude drugs preparationses to the various microorganism associated conditions of vagina.

2) lactic acid formulation: the vaginal secretions pH value raises during BV.The researcher employing lactic acid gel of Sweden improves and recovers the low acid environment of vagina, and part patient's vagina gram-positive bacillus (lactobacillus) is restored [7], but has research to think that the effect of lactic acid formulation is not so good as antibacterials [8].

3) lactobacillus preparation: the Gram-positive bacillus in the vagina mainly is a lactobacillus.Lactobacillus reduces or disappearance during vaginal dysbacteriosis, increases such as gram negative bacilli, negative cocci and gram-positive cocci.At the local lactobacillus that directly replenishes of vagina, can make the Gram-positive bacillus in the part patient vagina recover [9], but curative effect is stable inadequately, and the preservation of living preparation of lactobacillus preparation is relatively more difficult, goods viable count in storage process reduces very fast, has a strong impact on the effect duration [10] of goods.

International Patent Application WO 94/02148 discloses a kind of pharmaceutical composition for the treatment of vulvitis and vulvovaginitis, and prompting can promote the vagina epithelium tissue regeneration in mitigation symptoms.Its active component part is formed by 7-8 kind combinations of substances.Said composition contains lactose or the glucose of 3.0-15.0%.As the prospectus page 5 8-10 of this application capable as described in, lactose in the said composition or glucose are as carbon source.But do not mention in this application and can not have demonstration or hint said composition to have the effect that promotes growth of vagina gram-positive bacillus yet separately with the active component of sugar as treatment vulvitis and vagina vulvitis.In addition as the application prospectus page 5 17-18 capable as described in, this patent application emphasizes that very the pH value scope of compositions is 2-3.5.

United States Patent (USP) 3,860 discloses a kind of method for the treatment of trichomonal vaginitis and candidal vaginitis in 707.This method is to use lactulose (Lactulose) in vagina.Also mention in this patent lactulose can with such as carrier mixing administrations such as glucose, galactose or lactose.Wherein require the dense of lactulose, reach 50%, also have 5% lactose, 8% glucose simultaneously, as prospectus the 1st file 51-55 of application capable and the 5th file 1-5 capable as described in; The lactulose consumption is each 4-10 gram, every day 1-2 time, as apply for disclosed description the 4th file 63-66 capable as described in.This patent is not mentioned the effect of lactulose to vaginal microbial flora, do not point out lactulose can strengthen vagina acidity, reduce the vagina pH value, promote growth of vagina gram-positive bacillus, do not point out lactulose can treat and vaginal dysbacteriosis, vaginitis and the bacterial vaginosis of vagina acidity attenuation of correlation.Do not mention the therapeutical effect of glycogen yet.

European patent application EP-A-0257007 discloses a kind of vaginal treatment agent that contains lactic acid and buffering material and growth of lactobacillus substrate.As prospectus the 6th file 10-14 of this application capable with 25-55 capable as described in, the composition of this therapeutic agent is based on lactic acid, other contains a small amount of glycogen and lactose as growth substrate, wherein the weight ratio between lactic acid and glycogen or lactose is 20: 11500: 1, content of glycogen 0.1% (W/W) only in the medicament; And survival, growth to the vagina lactobacillus when this application does not have prompting to use glycogen as active component separately have assorted petty effect.Simultaneously as prospectus the 6th file 1-2 of this application capable as described in, emphasize that the pH value range regulation of medicament is very important to 3.5-4..Disclosed experiment in vitro result that it should be noted that this application demonstrates this medicament stronger selectivity bactericidal action, promptly strong to the effect of pathogen and a little less than the effect to lactobacillus; But lactobacillus just can be survived the long period in this medicament, but can not growth and breeding.Pointing out this medicament mainly is selective inhibitory to pathogenic bacteria to the mechanism of vaginal microbial flora Accommodation in vivo, but not directly promotes lactobacillus growth.

GB2112285A discloses a kind of vaginal lotion, and it contains and acts on pathogenic bacteria selectively and do not act on the disinfectant of lactobacillus and the nutrient of lactobacillus, and the pH value of washing liquid is as implementing to be illustrated as 5.71-6.2.Wherein nutrient has used the glucose and the unsaturated fatty acid of 1-2% (W/V), also loosely mentions monosaccharide and/or disaccharide.Can in this medicament, survive the long period but the experiment in vitro in the prospectus of this application only shows lactobacillus, and show that without any experimental data in external or the body this washing liquid has direct promotion lactobacillus growth and produces sour effect.When this application does not have prompting separately to be active component with glucose and any other saccharide to the effect of vaginal microbial flora.

The purpose of this invention is to provide a kind of by positive bacillus growth in the direct promotion patient vagina and produce that acid makes that vagina acidity and flora recover normally simultaneously, composition simply is easy to prepare, medicament easy to use, and uses this pharmaceutical treatment and the minimizing of vagina positive bacillus or vagina acidity to weaken the method for diseases associated.

A kind of by positive bacillus growth in the direct promotion patient vagina with produce acid, make that vagina acidity and flora recover normally simultaneously, composition medicine simple, easy to use in order to seek, the inventor has carried out extensive studies.Various medicaments to the prior art scheme experimentize, and all do not obtain the effect of direct promotion lactobacillus growth.But be surprised to find that very much, separately with glycogen as active component, can produce the effect that selectivity promotes growth and produces acid to lactobacillus; More surprisingly, although being higher than at 4.6 o'clock, the vagina pH value promptly is regarded as morbid state, but the medicament pH value of emphasizing with prior art must 4.0 or lower opposite, be that the medicament pH value scope of the present invention of active component is in 4.0＜pH＜7.3, especially during 4.8-5.4 with the glycogen, have the effect that promotes lactobacillus growth and product acid and the vagina pH value is reduced to below 4.6, almost do not have this effect and be lower than at 4.0 o'clock at pH value.Based on above-mentioned discovery, finished the present invention by the further research inventor.

The invention provides the water base medicament of a kind of direct promotion growth of vagina gram-positive bacillus, enhancing vagina acidity, it is characterized in that by the cumulative volume of medicament, it contains the glycogen of 2-18% (W/V); Also optionally contain pharmaceutically useful adhesive matrix; With acceptable acid of medicine or the alkali regulated in medicament pH value to 4.0＜pH＜7.3 scopes.

According to the present invention, glycogen content is 2-18% (W/V) in the medicament, and preferred content is 8-15% (W/V).

The application above and the weight/volume content of hereinafter mentioning (W/V) refer to gram number in per 100 milliliters of medicaments.

The pH scope of medicament of the present invention is 4.0＜pH＜7.3, and preferred pH value scope is 4.8-7.0.Wherein, thus medicament helps the stable decomposition of avoiding, reduce glycogen in the storage process of glycogen when neutral.The pH value of medicament can be regulated with acceptable acid of any medicine or alkali, preferred lactic acid and sodium hydroxide.

According to the present invention, can comprise the adhesive matrix composition in this medicament.Can select xanthan gum (XanthanGum) for use, concentration is 0.5-1.6% (W/V).Xanthan gum viscosity height, and, can make sugar contact more equably and stop the long period with vaginal mucosa to temperature, pH value vary stable, thus performance promotes the effect of positive bacillus growth and product acid.

Also available other adhesive carrier substrate well known by persons skilled in the art of this medicament, adjunct ingredient etc. are made gluey agent, ointment etc.

Glycogen is the primary activity composition in the medicament of the present invention, just can realize the object of the invention with the combination of suitable pharmaceutical carrier.But if add class material such as trace or a small amount of aminoacid, vitamin therein or be rich in the yeast extract etc. of aminoacid, vitamin, then effect is better.The micro-amino acid, the vitamin substances that lack in the vaginal secretions during because of experiment in vitro to be contained naturally are so must manually add a certain amount of aminoacid and vitamin.

The antifungal drug that also can contain effective dose in the medicament of the present invention, the conk that may occur after being used to prevent vagina acidity to strengthen increases.The example of antifungal drug has ketoconazole, triaconazole, itraconazole, fluconazol etc.

Also can contain the antibacterials to selective antibacterial, the bactericidal action of gram negative bacteria of effective dose in the medicament of the present invention, can strengthen the curative effect of medicament of the present invention like this vaginal infection or inflammation.The example of this anti-bacterial drug has polymyxin, metronidazole, aztreonam etc.

Medicament of the present invention can prepare by method known to those skilled in the art.

Can be with glycogen component, trace or aminoacid and vitamin component, matrix components mixing in a small amount, adding distil water stirs and makes saccharic composition dissolving, matrix components swelling be even viscosity glue,, gets final product through sterilizing to predetermined value with lactic acid or caustic lye of soda adjust pH again.Also can sterilize earlier, again adjust pH.Sterilization can be adopted discontinuous sterilization, and concrete grammar is: 80 degrees centigrade 30 minutes, put then 36 degrees Celsius 8-12 hour; Again 80 degrees centigrade 30 minutes, put then 36 the degree 8-12 hour; Again 80 degrees centigrade 30 minutes.Also can adopt 116 degrees centigrade of 15-20 minute autoclavings.

Glycogen in the medicament of the present invention weakens with temperature its stability that raises, so the medicament stored refrigerated is best, or puts shady and cool place and preserves.But the glycogen trace in the medicament, a small amount of decomposition do not influence its curative effect.

The invention still further relates to glycogen as the purposes of active component in the medicine of the direct promotion of preparation growth of vagina gram-positive bacillus, enhancing vagina acidity.

Medicament of the present invention prepares the acidity that medicine in the purposes can be used for directly promoting the growth of vagina positive bacillus and strengthens vagina, is used for the treatment of vagina acidity and weakens and weaken (pH＞4.6) relevant vaginitis, vaginal dysbacteriosis, particularly bacterial vaginosis with the vagina vagina acidity.

In body, experiment in vitro proves, medicament of the present invention has very strong direct promotion positive bacillus growth, produces the effect of acid, can be used for treating with the vagina positive bacillus reduce, vaginitis, vaginal dysbacteriosis and the bacterial vaginosis of vagina acidity attenuation of correlation.

Therefore, the invention still further relates to a kind of direct promotion growth of vagina gram-positive bacillus, strengthen vagina acidity, treat the vaginitis that vagina acidity weakens and be correlated with, the method for vaginal dysbacteriosis, especially bacterial vaginosis, comprising the invention described above medicament that has this patient who needs with the treatment effective dose.

Medication in medicament of the present invention and the Therapeutic Method of the present invention is the intravaginal topical.For the medicament of the present invention that contains adhesive matrix or other carrier matrix, can directly be applied to patient's intravaginal.Medicament of the present invention for the true solution dosage form can be used for soaking the vagina plug, and the vagina plug that soaks with medicament of the present invention is used for patient's intravaginal then.

Dosage in medicament of the present invention and the Therapeutic Method of the present invention is the each 3-5 milliliter of the medicament of the present invention that contains glycogen 8-15% (W/V), every day 1-3 time, makes medication every day total amount require the total sugar amount to reach the 0.24-2.25 gram.Normally medication every day twice, with time-out administration in morning, a few patients need add with a medicine noon before sleeping.The patient of vaginal microbial flora severely subnormal, its vaginal secretions pH＞5.0, smear staining checks that Gram-positive bacillus does not have or seldom, the medicine for treatment amount needs bigger, medication every day total sugar commercial weight is generally more than 0.8 gram; The patient that the state of an illness is lighter, its vaginal secretions 5.0＞pH＞4.6, the smear staining inspection has positive bacillus, but few than negative bacillus, negative cocci or positive coccus, the medicine for treatment amount is less, medication every day total sugar commercial weight is generally below 0.8 gram.

Should monitor the variation of patients clinical symptom, vagina pH value and vaginal microbial flora in the medication therapeutic process every day, and adjust dosage and medication number of times, course of treatment every day according to the change of illness state situation.In general, can produce significant curative effect with medicament 1-3 of the present invention days, patient symptom significantly takes a turn for the better or disappearance, the vagina pH value reduces to normal range and the vagina positive bacillus reverts to dominant microflora.Answer drug withdrawal, part patient need give low dose of medicament of the present invention and keep curative effect this moment.

In the method for the invention, can give the patient and only contain the medicament of glycogen, also can contain the medicament of the present invention of glycogen, antifungal drug and/or anti-bacterial drug as active component.Perhaps, can be used in combination with suitable antifungal drug or anti-bacterial drug as the medicament of active component only containing glycogen.Under latter event, medicament of the present invention finishes the back administration in the anti-bacterial drug treatment usually, is used to recover positive bacillus and vagina acidity; The administration simultaneously of antifungal drug medicament then common and of the present invention is used to prevent the fungus undue growth.

Patient's clinical symptoms is alleviated rapidly, and the intravaginal positive bacillus increases, the vagina pH value is reduced to 4.0-4.6, and harmful bacterium such as the excrescent negative bacillus of intravaginal, negative cocci significantly reduce even disappear.

The effectiveness of following EXPERIMENTAL EXAMPLE explanation medicament of the present invention of following reference or method.

The experiment in vitro 1 of medicament of the present invention

Experimental technique:

(1) prepares preparation: glycogen 10.0%, yeast extract powder 1.0%, xanthan gum 1.0%.According to aforementioned

Preparation method is prepared medicament, transfers pH5.0.Packing test tube after the sterilization treatment, every pipe 5 millis

Rise, standby.

(2) prepare specimen fluids: get typical bacteria vaginosis patient's vaginal secretions swab, immediately in 2

Eluting becomes suspension in the milliliter sterilization Semen sojae atricolor pancreatin peptone culture fluid, is specimen fluids.Should

Secretions is complicated flora, with multiple gram negative bacilli, the moon through the smear staining microscopy

The property coccus, and positive coccus be main, Gram-positive bacillus is not almost seen.

(3) inoculate the above-mentioned specimen fluids of just having got ready immediately in the medicament pipe, every pipe is inoculated 10 microlitres, mixes

Even, put 37 degrees centigrade of anaerobism and cultivate, respectively at getting the culture smear in 10 hours, 24 hours

Dyeing is observed, and surveys culture pH.

The result:

As shown in table 1, be inoculated into a large amount of gram negative bacilli, negative cocci undergrowth in the medicament of the present invention, and the Gram-positive bacillus raised growth becomes predominant bacteria, and preparation pH descends rapidly, show that this positive bacillus is to produce acid, acid fast bacteria.

Table 1. glycogen is cultivated preparation in the inoculum in the facilitation preparation of Gram-positive bacillus growth, product acid and was cultivated 24 hours sugar type bacterial species pH antibacterial pH antibacterial pH glycogen G+b in 10 hours,-5.0 G+b, +++4.8 G+b, +++4.2

G-b，++++ G-b，++ G-b，+

G-c，+++ G-c，- G-c，-

G+c，++ G+c，+ G+c，+

Conclusion: medicament of the present invention has very strong promotion growth to Gram-positive bacillus and promotes to produce the effect of acid; The growth of gram negative bacilli, negative cocci is subjected to remarkable inhibition.

Experiment embodiment 2

Model case research

Woods * *, women 35 years old.Leucorrhoea grow in quantity companion fish raw meat sample stink is 1 year repeatedly.The patient before 1 year no obvious inducement begin to occur leucorrhoea grow in quantity, companion Trichiurus haumela raw meat sample stink and pruritus vulvae.Once used multiple antibiotic therapy repeatedly, but symptom respite during medication, but stop the normal recurrence in treatment back.Get leucorrhea through the inventor and do the smear staining inspection, find that the vaginal microbial flora total amount increases, antibacterial is based on multiple gram negative bacilli and negative cocci, almost looks into to lose Gram-positive bacillus, also rarely found leukocyte; Surveying leucorrhea pH is 5.4.Be diagnosed as bacterial vaginosis, contain the medicament of the present invention of 12% glycogen, each 5 milliliters, every day twice, making medication total sugar commercial weight is about gram every days 1.2.Medication after one day leucorrhea amount promptly minimizing to some extent, stink and pruritus vulvae alleviate, look into leucorrhea pH4.6; Medication after 2 days leucorrhea reduce, stink disappears with pruritus vulvae, looks into leucorrhea pH4.1, checks leucorrhea smear staining microscopy, antibacterial is based on Gram-positive bacillus, negative bacillus, coccus are few.

The superiority that the present invention compares with background technology:

Compare with antibacterial therapy:

Antibacterials are from the nosetiology viewpoint, and employing sterilization or bacteriostatic method are controlled undue growth and caused the abnormal bacterial of pathological reaction.There is following main drawback in this approach: (1) use repeatedly antimicrobial drug behind the house antibacterial can produce drug resistance, curative effect is descended even invalid; (2) infection of easy secondary fastbacteria of antibacterial therapy etc.; (3) antibacterials have adverse reaction rates such as certain allergy and skin irritation.

With lactic acid, acetic acid and other selectivity antibacterial medicament that is active component, as medicament as described in patent application GB2112285A and the disclosed description of EP-A-0257007, the pathogenic bacteria inhibitory action is not had obvious bacteriostasis to lactobacillus by force, thereby help the growth of lactobacillus in the vagina indirectly.But these medicaments itself do not promote the effect of lactobacillus growth significantly.

Medicament of the present invention directly promotes growth of vagina gram-positive bacillus, strengthens vagina acidity, the growth by positive bacillus and produce this indirect mechanism of acid gram negative bacilli, coccus and gram-positive cocci are played inhibitory action.Thereby in fact the technology of the present invention and medicament strengthened the normal disease-resistant mechanism of vagina, and fundamentally avoid and overcome antibacterial drug therapy destroying drawbacks such as vagina normal flora, the obvious superiority of tool.

Compare with lactobacillus preparation:

At first, to heavier vaginal dysbacteriosis such as typical bacterial vaginosis example, the curative effect instability of lactobacillus preparation; Secondly, the production of Lemonal, preserve certain difficulty, cost is higher.Preparation production cost of the present invention is low, effect duration is long, significantly is better than lactobacillus preparation; And by improving the local microhabitat of vagina condition, promoting the inherent positive bacillus growth of patient's intravaginal, directly the supplemented with exogenous lactobacillus is better.

Medicament embodiment

Embodiment 1

With 2.0 gram glycogens and 1.6 gram xanthan gum mixings, add part distilled water and stirring, make saccharic composition dissolving, xanthan gum swelling be even viscosity glue; Add an amount of lactic acid and transfer the pH to 6.5 of solution, add distilled water once more to cumulative volume 100ml; Gained solution is sterilized with fractional sterilization.

Embodiment 2

Take by weighing raw material by following proportioning, the method by embodiment 1 has prepared the 100ml medicament substantially.Wherein transfer the sodium hydroxide solution of pH with 0.5N.

Glycogen 18.0% (W/V)

Yeast extract powder 1.0% (W/V)

Xanthan gum 0.5% (W/V)

The distilled water capacity

pH 7.28

Embodiment 3: take by weighing raw material by following proportioning, the method by embodiment 1 has prepared the 100ml medicament substantially:

Glycogen 12.0% (W/V)

Yeast extract powder 1.0% (W/V)

Xanthan gum 1.0% (W/V)

The distilled water capacity

pH 4.1

Embodiment 4: take by weighing raw material by following proportioning, the method by embodiment 1 has prepared the 100ml medicament substantially:

Glycogen 10.0% (W/V)

Histidine 100ppm

Methionine 50.0ppm

Riboflavin 0.2ppm

Thiamine 0.2ppm

Nicotinic acid 0.2ppm

Calcium pantothenate 0.2ppm

Xanthan gum 0.8% (W/V)

The distilled water capacity

pH 7.0

Medicine component of the present invention is simple, is easy to preparation, and is expensive low.

List of references 1) Eschenbach.DA, Clin.Infect.Dis.1993.16 (Supp1.4): S282-72) Thomason JL.Bacterial vaginosis:current review with indications for asymptomatic therapy.Am J Obstet Gynecol 1991; 165:1210.3) German-M, et al:Genital flora in pregnancy and its association with intrauterine growth retardation. J-Clin-Microbiol.1994 Sep:32 (9): 2162-84) McDonald-HM, et al:Vaginal infection and preterm labour.Br-J-Obstet-Gynaecol.1991 May; 98 (5): 427-355) Stein-GE, et al:Placebo-controlled trial of intravaginal clindamycin 2% cream for the treatment of bacterial vaginosis.Ann-Pharmacother.1993 Nov, 27 (11): 1343-56) Hillier-SL, et al:Efficacy of intravaginal 0.75% metronidazole gel for the treatment of bacterial vaginosis. Obstet-Gynecol.1993 Jun; 81 (6): 963-77) Hoster-E, et al:Treatment of bacterial vaginosis in pregnancy with a lactate gel.Scand-J-Infect-Dis.1990; 22 (5): 625-68) Boeke-AJ, et al:Effect of lactic acid suppositories compared with oral metronidazole and placebo in bacterial vaginosis:a randomised clinical trial.Genitourin-Med.1993 Oct; 69 (5): 388-929) Hallen-A, et al:Treatment of bacterial vaginosis with lactobacilli.Sex-Transm-Dis.1992 May-Jun; 19 (3): 146-810) Hughes-VL, et al:Microbiologic characteristics of lactobacillus products used for colonization of the vagina. Obstet-Gynecol.1990 Feb; 75 (2): 244-8

Claims (13)

1. a water base medicament that directly promotes growth of vagina gram-positive bacillus, strengthens vagina acidity is characterized in that by the medicament cumulative volume, it contains the glycogen of 2-18% (W/V); Also optionally contain pharmaceutically useful adhesive matrix; With acceptable acid of medicine or the alkali regulated in medicament pH value to 4.0＜pH＜7.3 scopes.

2. according to the medicament of claim 1, wherein glycogen content is 8-15% (W/V).

3. according to the medicament of claim 1, wherein the pH value scope of medicament is 4.8-7.0.

4. according to the medicament of claim 1, wherein contain the xanthan gum of 0.5-1.6% (W/V).

5. according to the medicament of claim 1, wherein also contain the antifungal of effective dose.

6. according to the medicament of claim 1, wherein also contain the medicine of the anti-gram negative bacteria of selectivity of effective dose.

7. glycogen directly promotes Gram-positive bacillus to grow, strengthen the purposes in the medicament of vagina acidity as active component in preparation.

8. according to the purposes of claim 7, wherein said medicine is to be used for the treatment of the medicine that vagina acidity weakens.

9. according to the purposes of claim 7, wherein said medicine is to be used for the treatment of and the vaginitis of vagina acidity attenuation of correlation, the medicine of vaginal dysbacteriosis.

10. according to the purposes of claim 7, wherein said medicine is the medicine that is used for the treatment of bacterial vaginosis.

11. a method that directly promotes growth of vagina gram-positive bacillus, strengthens vagina acidity wherein has this main body that needs with each medicament of the claim 1-6 of therapeutic dose effective dose.

12. a method for the treatment of vaginitis, vaginal dysbacteriosis wherein has this main body that needs with each medicament of the claim 1-6 of treatment effective dose.

13. a method for the treatment of bacterial vaginosis wherein has this main body that needs with each medicament of the claim 1-6 of treatment effective dose.