CN113862142A - PCR system capable of rapidly increasing and decreasing temperature and use method thereof - Google Patents
PCR system capable of rapidly increasing and decreasing temperature and use method thereof Download PDFInfo
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- CN113862142A CN113862142A CN202111232289.6A CN202111232289A CN113862142A CN 113862142 A CN113862142 A CN 113862142A CN 202111232289 A CN202111232289 A CN 202111232289A CN 113862142 A CN113862142 A CN 113862142A
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- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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Abstract
The invention discloses a PCR system capable of rapidly increasing and reducing temperature and a using method thereof, belonging to the technical field of PCR, and comprising a casing, a sample groove, a plurality of test tubes on the sample groove, wherein the test tubes are filled with samples, the PCR system also comprises a moving platform, two temperature control areas are distributed on the upper part of the moving platform, the two temperature control areas comprise a first temperature control area and a second temperature control area, a temperature control component is arranged in each temperature control area, the lower part of the moving platform is provided with a conveying structure, the conveying structure comprises a rack, a gear and a driving module, when the driving module works, the rack and the gear mutually drive the moving platform, and the moving platform drives each temperature control area to reciprocate. The structure scheme that the temperature control areas can be preheated is adopted, and the conveying structures such as the gear and the rack are combined, so that the sample tank is conveyed to and fro among different temperature control areas with preset temperatures, the time for heating and cooling the sample tank is shortened, and the heating and cooling rate in a PCR reaction circulation system is greatly improved.
Description
Technical Field
The invention belongs to the technical field of PCR, and particularly relates to a PCR system capable of quickly heating and cooling and a use method thereof.
Background
PCR (Polymerase Chain Reaction, short for short), is a molecular biology technique for amplifying and amplifying specific DNA fragments, which can be regarded as special DNA replication in vitro, and the biggest characteristic of PCR is that trace amount of DNA can be greatly increased. In modern molecular biology, the PCR technology has become a key technology of modern molecular biology experimental work and is widely applied to the fields of medicine, agriculture, inspection and quarantine and the like; because the PCR technology has high sensitivity and good specificity, the PCR technology is applied to relevant equipment carriers in gene detection practice, and is more and more important in the field of PCR technology development.
In the prior art, for example, chinese patent publication No. CN208717345U provides a PCR reaction apparatus, which includes a mounting base plate, a PCR reaction assembly, a low-temperature reagent storage assembly, and a gun head storage assembly are disposed on the mounting base plate, the PCR reaction assembly includes a reaction tank unit and a temperature control unit, the reaction tank unit is a carrying container for PCR reaction, and the temperature control unit is connected to the reaction tank unit and is configured to control a PCR reaction temperature in the reaction tank unit. The PCR reaction device adopts TEC (short for Thermo Electric Cooler), namely a thermoelectric refrigerator, to realize the control of the PCR reaction temperature. The positive and negative electrification is carried out on the thermoelectric cooler by utilizing the Peltier principle of the thermoelectric cooler so as to achieve the purpose of controlling the rapid temperature rise and fall of the sample tank: when the TEC passes through the forward current, the temperature of the hot surface is continuously increased; when the TEC passes through the reverse current, the hot surface becomes the cold surface, and the temperature is continuously reduced. However, the temperature control method depends on the performance of the TEC to a great extent, and the thermal loss of the TEC, the heat conductive silicone grease, the sample groove, the gap between the sample groove and the test tube, the wall of the test tube and other multi-layer interfaces increases the performance requirement on the TEC, increases the time required for reaching the set temperature, reduces the temperature rise and fall rate of the reaction system, and is difficult to meet the requirement of the PCR technology in the gene detection practical process.
Therefore, in order to solve the problems in the prior art, a solution for a PCR system with low energy consumption and capable of rapidly increasing and decreasing temperature is needed.
Disclosure of Invention
Aiming at the problems in the related art, the invention provides a PCR system capable of rapidly increasing and decreasing temperature and a using method thereof, so as to overcome the technical problems in the prior related art.
The technical scheme of the invention is realized as follows: a PCR system capable of rapidly heating and cooling comprises a casing and a sample groove arranged in the casing, wherein the sample groove is detachably and fixedly connected with the casing, a plurality of test tubes are uniformly distributed at intervals and are filled with samples to be subjected to PCR treatment,
the sample cell temperature control device also comprises a moving platform, two mutually independent temperature control areas are distributed on the upper part of the moving platform, the moving platform comprises a first temperature control area and a second temperature control area, a channel for the sample cell to pass through is arranged between the first temperature control area and the second temperature control area, a temperature control component is arranged in each temperature control area and is used for heating/cooling/keeping constant temperature treatment on the temperature control areas,
the lower part of moving platform is equipped with transport structure, transport structure includes rack, gear and drive module, the rack is fixed moving platform's lower terminal surface extends along two mutually independent control by temperature change region's distribution direction, the gear with form the meshing cooperation between the rack, drive module with gear drive is connected, and when drive module during operation, rack and gear mutual transmission drive moving platform, moving platform drives each control by temperature change region reciprocating motion, realizes that the sample groove shifts between first control by temperature change region and second control by temperature change region and switches.
According to the invention, by adopting the scheme of preheating each temperature control area and combining with conveying structures such as a gear rack and the like, the sample tank is conveyed to and fro between different temperature control areas with preset temperatures, so that heat transfer media in the process of transferring heat to the sample tank by the temperature control areas are greatly reduced, the heat loss is reduced, the sample tank can rapidly reach the required temperature, the required time for heating and cooling the sample tank is effectively shortened, and the heating and cooling rate and the control efficiency in a PCR reaction circulation system are greatly improved.
Preferably, the sample cell temperature control device further comprises a control unit, wherein the control unit is respectively connected with the temperature control assemblies and the driving modules in the temperature control areas, at least three preset circulating temperature values are stored in the control unit, and when the sample cell is arranged in the first temperature control area/the second temperature control area, the control unit controls the temperature control assemblies in the corresponding temperature control areas to carry out heating/cooling/constant temperature treatment according to the preset circulating temperature values until the temperature of the corresponding temperature control areas reaches the preset circulating temperature values;
it should be noted that the existing PCR instrument generally adopts a thermoelectric cooler to realize temperature control, but this temperature control method largely depends on the performance of the TEC itself, which increases the performance requirement on the TEC and increases the time required for reaching the set temperature; in order to effectively shorten the time required by the temperature rise and drop of the sample tank, a control unit is further arranged, at least three preset circulating temperature values are stored in the control unit, and before the sample tank does not enter a corresponding temperature control area, the temperature rise/drop/constant temperature treatment is carried out on the temperature control area to reach a required environmental temperature value, so that the temperature rise and drop rate of a reaction system is reduced, and the requirement of a PCR technology in the practical process is met.
Further preferably, the preset values of the circulating temperature are three, including a high-temperature denaturation temperature, a low-temperature renaturation temperature and a medium-temperature extension temperature,
the temperature range of the high-temperature denaturation temperature is 90-100 ℃, the temperature range of the low-temperature renaturation temperature is 50-60 ℃, and the temperature range of the medium-temperature extension temperature is 67-77 ℃;
it should be noted that, PCR is a molecular biology technique for amplifying and amplifying a specific DNA fragment, which can be regarded as a special DNA replication in vitro, and the greatest feature of PCR is that it can greatly increase a trace amount of DNA; the setting includes high temperature denaturation temperature, low temperature renaturation temperature and middle temperature extension temperature, can effectively satisfy the temperature demand of the special DNA duplication in vitro, realizes that the PCR instrument is used as a temperature control device, and well controls the polymerase chain reaction among denaturation temperature, renaturation temperature and extension temperature.
Preferably, the control unit further stores a preheating constant temperature preset value, and when the sample tank is placed in any one of the temperature control areas, the control unit controls the temperature control assembly in another adjacent temperature control area to perform heating/cooling/constant temperature processing according to the preheating constant temperature preset value until the temperature of the adjacent temperature control area reaches the preheating constant temperature preset value;
further preferably, the preheating constant temperature preset value is smaller than any one of the circulating temperature preset values;
it should be noted that, the conventional PCR instrument utilizes a thermoelectric cooler to realize temperature control of the PCR instrument, and in the actual use process, heat is greatly lost through the TEC, the heat conductive silicone grease, the sample cell, the gap between the sample cell and the test tube, the test tube wall, and other multilayer interfaces, even before the sample cell enters the corresponding temperature control region, the temperature control region is subjected to heating/cooling/constant temperature treatment, once the initial room temperature according to the temperature control region is lower, the reaction system of the PCR needs to be heated from the lower initial room temperature to the higher denaturation temperature/renaturation temperature/extension temperature, the heating and cooling rate of the reaction system of the PCR is seriously affected, and the application requirements of the PCR technology are difficult to meet; in order to avoid such defects of the PCR instrument, a preheating constant-temperature preset value is stored in the control unit, so that the temperature control area keeps a preheating temperature when being idle, and can quickly reach the required denaturation temperature/renaturation temperature/extension temperature when being started.
Preferably, the temperature control assembly includes a temperature sensor and a heating module, the temperature sensor identifies a temperature signal of the temperature control area, converts the temperature signal into an electrical signal, and transmits the electrical signal to the control unit, and the control unit correspondingly controls the heating module to perform heating/cooling/constant temperature processing on the temperature control area according to the electrical signal.
Preferably, the driving module comprises a motor, a driving belt pulley, a driven belt pulley, a belt and a main shaft, an output shaft of the motor is in driving connection with the driving belt pulley, the driving belt pulley is in driving connection with the driven belt pulley through the belt, the driven belt pulley is fixedly connected with the main shaft, the main shaft is in driving connection with the gear, and when the output shaft of the motor rotates, the driving belt pulley drives the driven belt pulley to rotate in a speed reduction manner to drive the gear to be in meshing transmission with the rack;
the gear rack is driven by the belt pulley transmission mode in a meshing transmission mode, so that the noise of the moving platform in the reciprocating conveying process can be effectively reduced, the moving platform can operate more stably and smoothly, and the purposes of buffering and absorbing vibration are achieved to a certain extent.
Further preferably, the number of teeth of the driven pulley is greater than that of the driving pulley; the ratio range of the number of teeth of the driven belt pulley to the number of teeth of the driving belt pulley is 4: 1-5: 1;
the PCR instrument achieves the purpose of easy maintenance and prolongs the actual service life of the PGR instrument by combining the conventional rotating speed and torque parameters of the motor in the prior art and selecting a proper reduction ratio on the premise of meeting the moving speed of the load of the moving platform.
Preferably, a semi-open coaming is further arranged at the peripheral edge of each temperature control area, and an open port of the coaming is communicated with the channel;
in order to enable temperature control areas on the moving platform to be mutually distinguished and independent, the enclosing plates are arranged, meanwhile, the arrangement of the enclosing plates can effectively avoid excessive heat from radiating outwards to be dissipated, a certain heat preservation effect is achieved, and energy consumption loss is reduced.
A method for using a PCR system capable of rapidly increasing and decreasing temperature is applied to the PCR system, and comprises the following operation steps:
step 4, heating the temperature control assembly of the first temperature control area in advance to enable the temperature in the first temperature control area to reach the required medium-temperature extension temperature, wherein the temperature range is 67-77 ℃; after the PCR low-temperature renaturation stage is finished, the driving module drives the gear rack to transmit to drive the two temperature control areas to move relative to the sample tank, so that the sample tank enters the first temperature control area again, and the sample in the sample tank enters a PCR medium-temperature extension stage;
and 5, circularly switching the sample tank between the two temperature control areas in such a way, and rapidly heating and cooling the three temperature stages of the PCR system.
The invention has the beneficial effects that:
compared with a TEC temperature control mode adopted by common PCR instrument equipment, the invention adopts a structural scheme that a temperature control area can be preheated, and combines a gear rack and other conveying structures to convey the sample tank to and fro between different temperature control areas with preset temperatures, thereby greatly reducing heat transfer media in the process of transferring heat to the sample tank by the temperature control area, reducing heat loss, simultaneously enabling the sample tank to rapidly reach the required temperature, effectively shortening the time for increasing and decreasing the temperature of the sample tank, and greatly improving the temperature increasing and decreasing rate and the control efficiency in a PCR reaction circulation system.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a front view of the present invention;
FIG. 3 is a top view of the present invention;
FIG. 4 is a schematic diagram of the operation of the sample cell of the present invention in a high temperature denaturation stage in a first temperature control zone;
FIG. 5 is a schematic diagram of the sample cell of the present invention disposed in a second temperature controlled region for low temperature renaturation stage;
FIG. 6 is a schematic diagram of the operation of the sample cell of the present invention in the middle temperature extension stage in the first temperature controlled zone;
reference numerals:
1. a sample tank; 11. a test tube;
2. a mobile platform; 21. a first temperature control region; 22. a second temperature control region; 23. enclosing plates; t1, channel;
3. a conveying structure; 31. a rack; 32. a gear; 33. a drive module; 331. a motor; 332. a drive pulley; 333. a belt; 334. a driven pulley; 335. a main shaft.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1-6, a PCR system capable of rapidly increasing and decreasing temperature includes a housing (not shown in the figures), and a sample tank 1 disposed in the housing, where the sample tank 1 is detachably and fixedly connected to the housing, the sample tank 1 is provided with a plurality of test tubes 11 uniformly distributed at intervals, the test tubes 11 are filled with samples to be subjected to PCR, specifically, the sample tank 1 is provided with a plurality of slots (not shown in the figures) uniformly distributed at intervals, and correspondingly, the test tubes 11 are embedded into the slots for fixation, bottoms of the test tubes 11 have a gap with end faces of temperature control areas related below, in this embodiment, the number of the test tubes 11 is preferably 96, the distance between the test tubes 11 and a central axis is 8 mm-10 mm, and a suitable distance between two test tubes 11 is set, so that thermal radiation interference between two test tubes 11 can be effectively avoided, meets the requirements of the PCR technology in the practical process of gene detection.
It should be noted that PCR (Polymerase Chain Reaction), is a molecular biology technique for amplifying and amplifying a specific DNA fragment, wherein a sample includes a minute amount of DNA to be replicated.
The sample cell device further comprises a moving platform 2, wherein two independent temperature control areas are distributed at the upper part of the moving platform 2, the two independent temperature control areas comprise a first temperature control area 21 and a second temperature control area 22, a channel T1 for the sample cell 1 to pass through is arranged between the first temperature control area 21 and the second temperature control area 22, in the embodiment, a semi-open coaming plate 23 is further arranged at the peripheral edge of each temperature control area, and an open port of the coaming plate 23 is communicated with the channel T1; in order to enable temperature control areas on the mobile platform 2 to be mutually distinguished and independent, the enclosing plate 23 is arranged, meanwhile, the arrangement of the enclosing plate 23 can effectively avoid excessive heat from radiating outwards to be dissipated, a certain heat preservation effect is achieved, and energy consumption loss is reduced.
Each temperature control area is internally provided with a temperature control assembly (not shown in the figure) for heating/cooling/maintaining the temperature of the temperature control area, in this embodiment, the temperature control assembly includes a temperature sensor (not shown in the figure) and a heating module (not shown in the figure), the temperature sensor identifies a temperature signal of the temperature control area, converts the temperature signal into an electrical signal, and transmits the electrical signal to the control unit, and the control unit correspondingly controls the heating module to heat/cool/maintain the temperature of the temperature control area according to the electrical signal.
In this embodiment, the heating module includes the hot plate, be provided with a plurality of metal coil on the hot plate, control the temperature through metal coil's electrically conductive heating principle and handle, it is specific, the PCR system adopts the air as the heat conduction medium, carries out heat conduction treatment to two temperature control regions respectively, therefore the temperature homogeneity is good, each hole amplification efficiency highly uniform.
The lower part of the moving platform 2 is provided with a conveying structure 3, the conveying structure 3 comprises a rack 31, a gear 32 and a driving module 33, the rack 31 is fixed on the lower end face of the moving platform 2 and extends along the distribution direction of two independent temperature control areas, the gear 32 and the rack 31 form meshing fit, the driving module 33 is in driving connection with the gear 32, when the driving module 33 works, the gear 32 and the rack 31 mutually transmit and drive the moving platform 2, the moving platform 2 drives each temperature control area to reciprocate, and the sample tank 1 is transferred and switched between the first temperature control area 21 and the second temperature control area 22;
specifically, the number of teeth of gear 32 is greater than 17, and in this embodiment, the number of teeth of preferred gear 32 is 42, and the number of teeth that sets up gear 32 is greater than 17, can avoid gear 32's undercut phenomenon effectively, when guaranteeing gear 32 transmission efficiency, prolongs gear 32's actual life.
In this embodiment, the driving module 33 includes a motor 331, a driving pulley 332, a driven pulley 334, a belt 333 and a spindle 335, an output shaft of the motor 331 is in driving connection with the driving pulley 332, the driving pulley 332 is in driving connection with the driven pulley 334 through the belt 333, the driven pulley 334 is fixedly connected with the spindle 335, the spindle 335 is in driving connection with the gear 32, when the output shaft of the motor 331 rotates, the driving pulley 332 drives the driven pulley 334 to decelerate and rotate, and the gear 32 is driven to engage with the rack 31 for transmission;
the meshing transmission of the gear 32 and the rack 31 is driven by adopting a belt 333 wheel transmission mode, so that the noise of the moving platform 2 in the reciprocating conveying process can be effectively reduced, the operation of the moving platform 2 is more stable and smooth, and the aims of buffering and absorbing vibration are fulfilled to a certain extent.
In a preferred embodiment, the number of teeth of the driven pulley 334 is greater than that of the driving pulley 332; the range of the ratio of the number of teeth of the driven pulley 334 to the number of teeth of the driving pulley 332 is 4: 1-5: 1;
specifically, the number of teeth of the driven pulley 334 in this embodiment is 72, the number of teeth of the driving pulley 332 is 16, and the PCR instrument achieves the purpose of easy maintenance and prolongs the actual service life of the PGR instrument by combining the conventional rotation speed and torque parameters of the motor 331 in the prior art and selecting an appropriate reduction ratio on the premise of satisfying the moving speed of the load of the mobile platform 2.
In this embodiment, the sample cell further includes a control unit (not shown), the control unit is respectively connected to the temperature control assemblies and the driving module 33 in the temperature control areas, at least three preset values of circulating temperature are stored in the control unit, and when the sample cell 1 is placed in the first temperature control area 21/the second temperature control area 22, the control unit controls the temperature control assemblies in the corresponding temperature control areas to perform heating/cooling/constant temperature processing according to the preset values of circulating temperature until the temperature of the corresponding temperature control areas reaches the preset values of circulating temperature;
it should be noted that the existing PCR instrument generally adopts a thermoelectric cooler to realize temperature control, but this temperature control method largely depends on the performance of the TEC itself, which increases the performance requirement on the TEC and increases the time required for reaching the set temperature; in order to effectively shorten the time required by the temperature rise and decrease of the sample tank 1, a control unit is further arranged, at least three preset circulating temperature values are stored in the control unit, and before the sample tank 1 enters a corresponding temperature control area, the temperature rise/decrease/constant temperature treatment is carried out on the temperature control area to reach a required environmental temperature value, so that the temperature rise and decrease rate of a reaction system is reduced, and the requirement of a PCR technology in a practical process is met.
Specifically, the preset values of the circulating temperature are three, including a high-temperature denaturation temperature, a low-temperature renaturation temperature and a medium-temperature extension temperature,
the temperature range of the high-temperature denaturation temperature is 90-100 ℃, and the preferred temperature of the embodiment is 95 ℃;
the temperature range of the low-temperature renaturation temperature is 50-60 ℃, and the preferred temperature of the embodiment is 55 ℃;
the temperature range of the medium-temperature extension temperature is 67-77 ℃; the preferred temperature for this embodiment is 72 ℃;
it should be noted that, PCR is a molecular biology technique for amplifying and amplifying a specific DNA fragment, which can be regarded as a special DNA replication in vitro, and the greatest feature of PCR is that it can greatly increase a trace amount of DNA; the setting includes high temperature denaturation temperature, low temperature renaturation temperature and middle temperature extension temperature, can effectively satisfy the temperature demand of the special DNA duplication in vitro, realizes that the PCR instrument is used as a temperature control device, and well controls the polymerase chain reaction among denaturation temperature, renaturation temperature and extension temperature.
In this embodiment, a preset preheating constant temperature value is further stored in the control unit, and when the sample tank 1 is placed in any one of the temperature control areas, the control unit controls the temperature control assembly in another adjacent temperature control area to perform heating/cooling/constant temperature processing according to the preset preheating constant temperature value until the temperature of the adjacent temperature control area reaches the preset preheating constant temperature value;
specifically, the preheating constant temperature preset value is smaller than any one of the circulating temperature preset values; in this embodiment, the temperature is preferably 30 to 40 ℃.
It should be noted that, the conventional PCR instrument utilizes a thermoelectric cooler to realize temperature control of the PCR instrument, and in the actual use process, heat is greatly lost through the TEC, the heat conductive silicone grease, the sample cell 1, the gap between the sample cell 1 and the test tube 11, and the wall of the test tube 11, etc. in this temperature control manner, even before the sample cell 1 enters the corresponding temperature control region, the temperature control region is subjected to temperature rise/drop/constant temperature treatment, once the initial room temperature according to the temperature control region is lower, the reaction system of the PCR needs to be heated from the lower initial room temperature to the higher denaturation temperature/renaturation temperature/extension temperature, which seriously affects the temperature rise and fall rate of the reaction system of the PCR, and is difficult to meet the application requirements of the PCR technology; in order to avoid such defects of the PCR instrument, a preheating constant-temperature preset value is stored in the control unit, so that the temperature control area keeps a preheating temperature when being idle, and can quickly reach the required denaturation temperature/renaturation temperature/extension temperature when being started.
Through the above scheme of the embodiment, in a specific application,
as shown in fig. 4 to 6, a method for using a PCR system capable of rapidly increasing and decreasing temperature includes the following steps:
step 4, heating the temperature control assembly of the first temperature control area 21 in advance to enable the temperature in the first temperature control area 21 to reach the required medium-temperature extension temperature, wherein the temperature range is 67-77 ℃; after the PCR low-temperature renaturation stage is finished, the driving module 33 drives the gear 32 and the rack 31 to transmit, so that the two temperature control areas are driven to move relative to the sample tank 1, the sample tank 1 enters the first temperature control area 21 again, and the sample in the sample tank 1 enters a PCR medium-temperature extension stage;
and 5, circularly switching the sample tank 1 between the two temperature control areas in such a way, and rapidly heating and cooling the three temperature stages of the PCR system.
In this embodiment, through having adopted the scheme of preheating each control by temperature change region, combine transport structure 3 such as gear 32 rack 31 simultaneously, with sample cell 1 reciprocating transport between the different control by temperature change regions of preset temperature, the heat transfer medium of the control by temperature change region in-process of giving sample cell 1 heat transfer that has significantly reduced, when reducing calorific loss, make sample cell 1 can reach required temperature rapidly, effectively shortened sample cell 1 and gone up and down the required length of time of temperature, the speed of going up and down the temperature and the control efficiency in the PCR reaction circulation system have been improved greatly.
Variations and modifications to the above-described embodiments may occur to those skilled in the art, which fall within the scope and spirit of the above description. Therefore, the present invention is not limited to the specific embodiments disclosed and described above, and some modifications and variations of the present invention should fall within the scope of the claims of the present invention. Furthermore, although specific terms are employed herein, they are used in a generic and descriptive sense only and not for purposes of limitation.
Claims (10)
1. A PCR system capable of rapidly heating and cooling comprises a casing and a sample groove arranged in the casing, wherein the sample groove is detachably and fixedly connected with the casing, a plurality of test tubes are uniformly distributed at intervals and are filled with samples to be subjected to PCR treatment,
the sample cell temperature control device also comprises a moving platform, two mutually independent temperature control areas are distributed on the upper part of the moving platform, the moving platform comprises a first temperature control area and a second temperature control area, a channel for the sample cell to pass through is arranged between the first temperature control area and the second temperature control area, a temperature control component is arranged in each temperature control area and is used for heating/cooling/keeping constant temperature treatment on the temperature control areas,
the lower part of moving platform is equipped with transport structure, transport structure includes rack, gear and drive module, the rack is fixed moving platform's lower terminal surface extends along two mutually independent control by temperature change region's distribution direction, the gear with form the meshing cooperation between the rack, drive module with gear drive is connected, and when drive module during operation, rack and gear mutual transmission drive moving platform, moving platform drives each control by temperature change region reciprocating motion, realizes that the sample groove shifts between first control by temperature change region and second control by temperature change region and switches.
2. The PCR system of claim 1, further comprising a control unit, wherein the control unit is respectively connected to the temperature control assemblies and the driving module in each temperature control area, at least three preset circulating temperature values are stored in the control unit, and when the sample tank is placed in the first temperature control area/the second temperature control area, the control unit controls the temperature control assemblies in the corresponding temperature control areas to perform heating/cooling/constant temperature processing according to the preset circulating temperature values until the temperature of the corresponding temperature control areas reaches the preset circulating temperature values.
3. The PCR system of claim 2, wherein the preset cycling temperature values are three, including a high temperature denaturation temperature, a low temperature renaturation temperature, and a medium temperature extension temperature,
the temperature range of the high-temperature denaturation temperature is 90-100 ℃, the temperature range of the low-temperature renaturation temperature is 50-60 ℃, and the temperature range of the medium-temperature extension temperature is 67-77 ℃.
4. The PCR system of claim 3, wherein the control unit further stores a pre-heating constant temperature preset value, and when the sample tank is placed in any one of the temperature controlled zones, the control unit controls the temperature control assembly in another adjacent temperature controlled zone to perform heating/cooling/constant temperature treatment according to the pre-heating constant temperature preset value until the temperature of the adjacent temperature controlled zone reaches the pre-heating constant temperature preset value.
5. The PCR system of claim 4, wherein the pre-heat constant temperature preset value is less than any of the cycling temperature preset values.
6. The PCR system of claim 5, wherein the temperature control assembly comprises a temperature sensor and a heating module, the temperature sensor identifies a temperature signal of the temperature control area, converts the temperature signal into an electrical signal and transmits the electrical signal to the control unit, and the control unit correspondingly controls the heating module to heat/cool/keep the temperature of the temperature control area according to the electrical signal.
7. The PCR system of claim 1, wherein the driving module comprises a motor, a driving pulley, a driven pulley, a belt and a main shaft, an output shaft of the motor is in driving connection with the driving pulley, the driving pulley is in belt transmission connection with the driven pulley, the driven pulley is fixedly connected with the main shaft, the main shaft is in driving connection with the gear, and when the output shaft of the motor rotates, the driving pulley drives the driven pulley to rotate at a reduced speed to drive the gear to perform meshing transmission with the rack.
8. The PCR system of claim 7 wherein the driven pulley has a greater number of teeth than the drive pulley; the ratio range of the number of teeth of the driven belt pulley to the number of teeth of the driving belt pulley is 4: 1-5: 1.
9. the PCR system of claim 1, wherein the peripheral edge of each temperature control region is further provided with a semi-open enclosure, and the open port of the enclosure communicates with the channel.
10. Use method of a PCR system capable of rapidly increasing and decreasing temperature, which is applied to the PCR system of any one of claims 1 to 9, comprising the following steps:
step 1, when a sample tank is subjected to a PCR system circulation stage of a preset program, heating a temperature control assembly of a first temperature control area at a preheating constant temperature in advance to enable the first temperature control area to rapidly reach a high-temperature denaturation temperature within a temperature range of 90-100 ℃, driving a gear rack to transmit by a driving module to drive the two temperature control areas to move relative to the sample tank, enabling the sample tank to enter the first temperature control area, and enabling a sample in the sample tank to enter a PCR high-temperature denaturation stage;
step 2, heating the temperature control assembly of the second temperature control area in advance to enable the temperature in the second temperature control area to reach the required low-temperature renaturation temperature, wherein the temperature range is 50-60 ℃;
step 3, when the PCR high-temperature denaturation stage is finished, the driving module drives the gear rack to transmit, and drives the two temperature control areas to move relative to the sample tank, so that the sample tank quickly enters a second temperature control area, the sample in the sample tank quickly reaches the low-temperature renaturation temperature of the second temperature control area, and the PCR low-temperature renaturation stage is started;
step 4, heating the temperature control assembly of the first temperature control area in advance to enable the temperature in the first temperature control area to reach the required medium-temperature extension temperature, wherein the temperature range is 67-77 ℃; after the PCR low-temperature renaturation stage is finished, the driving module drives the gear rack to transmit to drive the two temperature control areas to move relative to the sample tank, so that the sample tank enters the first temperature control area again, and the sample in the sample tank enters a PCR medium-temperature extension stage;
and 5, circularly switching the sample tank between the two temperature control areas in such a way, and rapidly heating and cooling the three temperature stages of the PCR system.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111232289.6A CN113862142A (en) | 2021-10-22 | 2021-10-22 | PCR system capable of rapidly increasing and decreasing temperature and use method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111232289.6A CN113862142A (en) | 2021-10-22 | 2021-10-22 | PCR system capable of rapidly increasing and decreasing temperature and use method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115113662A (en) * | 2022-06-23 | 2022-09-27 | 中元汇吉生物技术股份有限公司 | Gene amplification instrument, control method thereof, and computer-readable storage medium |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115113662A (en) * | 2022-06-23 | 2022-09-27 | 中元汇吉生物技术股份有限公司 | Gene amplification instrument, control method thereof, and computer-readable storage medium |
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Application publication date: 20211231 |
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