CN113693189B - Application and method of manganese peroxidase for degrading patulin - Google Patents
Application and method of manganese peroxidase for degrading patulin Download PDFInfo
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- CN113693189B CN113693189B CN202111139277.9A CN202111139277A CN113693189B CN 113693189 B CN113693189 B CN 113693189B CN 202111139277 A CN202111139277 A CN 202111139277A CN 113693189 B CN113693189 B CN 113693189B
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- Prior art keywords
- patulin
- ala
- manganese peroxidase
- pro
- gly
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- 230000000593 degrading effect Effects 0.000 title claims abstract description 17
- 238000000034 method Methods 0.000 title claims abstract description 14
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- 125000003275 alpha amino acid group Chemical group 0.000 claims abstract 3
- 235000015197 apple juice Nutrition 0.000 claims description 5
- 230000015556 catabolic process Effects 0.000 abstract description 15
- 238000006731 degradation reaction Methods 0.000 abstract description 15
- 231100000678 Mycotoxin Toxicity 0.000 abstract description 9
- 239000002636 mycotoxin Substances 0.000 abstract description 9
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- 231100000765 toxin Toxicity 0.000 abstract 1
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- 239000012452 mother liquor Substances 0.000 description 1
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- DAEYIVCTQUFNTM-UHFFFAOYSA-N ochratoxin B Natural products OC1=C2C(=O)OC(C)CC2=CC=C1C(=O)NC(C(O)=O)CC1=CC=CC=C1 DAEYIVCTQUFNTM-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/25—Removal of unwanted matter, e.g. deodorisation or detoxification using enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/84—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y111/00—Oxidoreductases acting on a peroxide as acceptor (1.11)
- C12Y111/01—Peroxidases (1.11.1)
- C12Y111/01013—Manganese peroxidase (1.11.1.13)
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Nutrition Science (AREA)
- Molecular Biology (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The invention relates to the field of biotechnology, and discloses application and a method of manganese peroxidase for degrading patulin. The invention provides application of manganese peroxidase with an amino acid sequence shown as SEQ ID NO. 1 to degradation of patulin. The manganese peroxidase from Moniliophthora roreri source can efficiently degrade mycotoxin patulin, and the method has low cost and wide application range and can be widely applied to the field of food toxin degrading enzymes.
Description
Technical Field
The invention relates to the field of biotechnology, and discloses application and a method of manganese peroxidase for degrading patulin.
Background
Mycotoxins are toxic secondary metabolites produced by fungi and are potentially harmful to food safety. Patulin is one of the most widely transmitted mycotoxins in agricultural products. Patulin is commonly associated with apples and apple products, however, it is also found in other fruits and vegetables, such as pears, figs, tomatoes, and the like. Scientific investigation also found patulin contamination in cereals such as wheat, rice and corn. Patulin poses many health risks to humans and animals, including mutagenesis, teratogenesis, and carcinogenesis.
Traditional methods of controlling mycotoxins include physical, chemical and biological methods. There are reports of degradation of mycotoxins by enzymes, such as degradation of zearalenone by dye decolorization peroxidase. Different peroxidases degrade mycotoxins differently. Manganese peroxidases have been reported to degrade aflatoxins, but manganese peroxidases have not been reported to degrade other mycotoxins. Patulin degrading enzymes may have great commercial potential in detoxification in foods. However, so far few patulin degrading enzymes have been isolated and identified.
Disclosure of Invention
The invention aims to provide an application of manganese peroxidase in degradation of patulin.
It is a further object of the present invention to provide a method for degrading patulin in a foodstuff.
The manganese peroxidase is Moniliophthora roreri-source MrMnP, and the amino acid sequence of the manganese peroxidase is shown as SEQ ID NO. 1. The method for degrading patulin in food according to the invention comprises the step of degrading patulin by manganese peroxidase with an amino acid sequence shown in SEQ ID NO. 1.
The method of degrading patulin in a foodstuff according to the invention, wherein the foodstuff is an apple-derived foodstuff, such as apple juice or apple jam.
The embodiment of the invention shows that the degradation rate of the manganese peroxidase from the cocoa gray fruit rot germ Moniliophthora roreri on the patulin reaches 100%, but the mycotoxin ochratoxin cannot be degraded, so that the enzyme can degrade the mycotoxin patulin efficiently and specifically.
Drawings
FIG. 1 shows the results of HPLC analysis of pure product of manganese peroxidase-degraded patulin;
FIG. 2 shows the HPLC analysis of manganese peroxidase to degrade patulin in monascus red;
fig. 3 shows the results of HPLC analysis of pure ochratoxin degradation by manganese peroxidase.
Detailed Description
Test materials and reagents
1. Strains: pichia pastoris engineering strain for producing Moniliophthora roreri source manganese peroxidase MrMnP.
2. Biochemical reagent: patulin; acetonitrile was chromatographically pure.
3. Culture medium: yeast medium YPD (2% peptone, 1% yeast extract, 1% glucose); culture medium BMGY (2% peptone, 1% yeast extract, 1% glycerol, 10% YNB solution, 1% biotin solution); culture medium BMMY (2% peptone, 1% yeast extract, 1% methanol, 10% YNB solution, 1% biotin solution).
EXAMPLE 1 preparation of recombinant manganese peroxidase MrMnP
The Moniliophthora roreri-derived MrMnP gene sequence was synthesized entirely from Jin Weizhi. Taking X33/MrMnP Pichia pastoris engineering strain containing recombinant plasmid, inoculating the Pichia pastoris engineering strain into 50 mL YPD culture solution, shaking and culturing the Pichia pastoris engineering strain at 30 ℃ and 220 rpm for 48h, transferring the Pichia pastoris engineering strain into 300 mL BMGY culture medium according to a proportion of 2%, shaking and culturing the Pichia pastoris engineering strain at 30 ℃ and 220 rpm for 48h, centrifuging the BMGY yeast culture solution at 5,500 rpm for 5 min, and discarding the supernatant. 200 mL of BMMY medium (heme at a final concentration of 100. Mu.M) was added to the flask. Shake culturing at 30deg.C and 200 rpm for 48h, centrifuging at 5,500 rpm for 5 min, and collecting fermentation broth to obtain recombinant manganese peroxidase MrMnP.
EXAMPLE 2 degradation of patulin by manganese peroxidase
The patulin is dissolved in acetonitrile to prepare a mother solution of 5g/L, and the reaction system is as follows: 250. mu.L of malonic acid buffer (0.2M, pH 5.0), 100. Mu.L of patulin solution (50 mg/L), 250. Mu.L of manganese sulfate (40 mM), 250. Mu.L of manganese peroxidase prepared in example 1 (5000U/L), 200. Mu.L of hydrogen peroxide (5 mM). The reaction system was repeated 3 times with the control system without manganese peroxidase. The reaction was carried out at 30℃and after 12. 12 h, three volumes of methanol were added to terminate the reaction, and the degradation rate of patulin was analyzed by High Performance Liquid Chromatography (HPLC). The liquid chromatography is a Shimadzu Nexera UHPLC high performance liquid chromatography analysis system, the chromatographic separation column is Zorbax SB-C18 (4.6X1250 mm, 5 μm), mobile phase A (0.1% acetic acid water) and mobile phase B (acetonitrile); eluting for 20 minutes under the elution condition of 10% B; the detection wavelength 276 nm. As a result, as shown in FIG. 1, patulin was degraded completely, with a degradation rate of 100%.
EXAMPLE 3 manganese peroxidase degradation of patulin in fruit juice
The patulin is dissolved in acetonitrile to prepare a mother solution with the concentration of 5g/L, and the reaction system is as follows: 250. mu.L of malonic acid buffer (0.2M, pH 5.0, apple juice as solvent), 25. Mu.L of patulin solution (200 mg/L), 250. Mu.L of manganese sulfate (40 mM, apple juice as solvent), 125. Mu.L of manganese peroxidase prepared in example 1 (10000U/L), 100. Mu.L of hydrogen peroxide (10 mM), 250. Mu.L of apple juice. The reaction system was repeated 3 times with the control system without manganese peroxidase. The reaction was carried out at 30℃and terminated by adding three volumes of methanol after 24. 24 h, and the degradation rate of patulin was analyzed by High Performance Liquid Chromatography (HPLC). The liquid chromatography is a Shimadzu Nexera UHPLC high performance liquid chromatography analysis system, the chromatographic separation column is Zorbax SB-C18 (4.6X1250 mm, 5 μm), mobile phase A (0.1% acetic acid water) and mobile phase B (acetonitrile); eluting for 20 minutes under the elution condition of 10% B; the detection wavelength 276 nm. As a result, as shown in FIG. 2, most of patulin was degraded, and the degradation rate was 96%.
EXAMPLE 4 manganese peroxidase degradation of ochratoxins
1 mg ochratoxin A powder was dissolved in 1 mL DMSO to make 1 mg/mL of mother liquor. The following reaction system is adopted: 250. mu.L of malonic acid buffer (0.2M, pH 5.0), 100. Mu.L of ochratoxin solution (500 mg/L), 250. Mu.L of manganese sulfate (40 mM), 250. Mu.L of manganese peroxidase prepared in the example (5000U/L), 200. Mu.L of hydrogen peroxide (5 mM). The reaction system was repeated 3 times with the control system without manganese peroxidase. The reaction was carried out at 30℃and after 12. 12 h, three volumes of methanol were added to terminate the reaction, and the degradation rate of patulin was analyzed by High Performance Liquid Chromatography (HPLC). The liquid chromatography is a Shimadzu Nexera UHPLC high performance liquid chromatography analysis system, the chromatographic separation column is Zorbax SB-C18 (4.6X1250 mm, 5 μm), mobile phase A (0.1% acetic acid water) and mobile phase B (acetonitrile); eluting for 20 minutes under the condition of 48% B; excitation wavelength 333nm and emission wavelength 460nm. The results are shown in FIG. 3, and the manganese peroxidase MrMnP has no obvious degradation effect on ochratoxin.
Sequence listing
<110> Beijing livestock veterinary research institute of China agricultural sciences
Application and method of <120> manganese peroxidase for degrading patulin
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 344
<212> PRT
<213> Alternaria alternata (Moniliophthora roreri)
<400> 1
Met Ala Val Pro Gln Arg Val Ala Cys Ala Asp Gly Val His Thr Ala
1 5 10 15
Ser Asn Ala Ala Cys Cys Ala Leu Phe Pro Ile Val Asp Val Leu Gln
20 25 30
Ser Asp Phe Phe Asp Gly Gly Glu Cys Gly Glu Glu Ala His Glu Ser
35 40 45
Leu Arg Leu Thr Phe His Asp Ala Ile Gly Phe Ser Pro Thr Leu Gly
50 55 60
Gly Gly Gly Ala Asp Gly Ser Ile His Val Phe Ser Asp Ile Glu Thr
65 70 75 80
Ala Phe His Ala Asn Gly Gly Ile Asp Glu Ile Val Asp Ala Gln Lys
85 90 95
Ala Phe Ile Ala Gln His Asn Ile Thr Ile Ser Pro Gly Asp Phe Ile
100 105 110
Gln Leu Ala Gly Ala Val Gly Leu Ser Asn Cys Pro Gly Ala Pro Arg
115 120 125
Leu Asn Phe Phe Phe Gly Arg Pro Pro Pro Lys Ala Ala Ala Pro Asp
130 135 140
Gly Leu Ile Pro Glu Pro Phe Asp Ser Val Thr Lys Ile Leu Asn Arg
145 150 155 160
Phe Ala Asp Ala Gly Phe Asn Ser Lys Glu Val Ile Ala Leu Leu Ala
165 170 175
Ser His Ser Val Ala Ala Ala Asp Lys Val Asp Pro Ser Ile Pro Gly
180 185 190
Thr Pro Phe Asp Ser Thr Pro Gly Ile Phe Asp Ser Gln Phe Phe Ile
195 200 205
Glu Val Gln Leu Arg Gly Thr Ala Phe Pro Gly Pro Asn Ser Thr Ala
210 215 220
Pro Ala Thr Asp Gly Glu Ala Glu Ser Pro Leu Arg Gly Glu Met Arg
225 230 235 240
Ile Ser Ser Asp Glu Asp Leu Ala Arg Asp Pro Arg Thr Ala Cys Glu
245 250 255
Trp Gln Ser Phe Val Asn Asn Gln Ala Lys Met Gln Thr Ala Phe Lys
260 265 270
Ala Ala Met Asn Lys Leu Ala Val Leu Gly Gln Asp Arg Arg Arg Leu
275 280 285
Ile Asp Cys Ser Glu Val Ile Pro Thr Thr Lys Pro Val Val Gly Arg
290 295 300
Ala His Leu Pro Ala Gly Ala Ser Arg Ala Asp Val Gln Gln Ala Cys
305 310 315 320
Ala Thr Ser Pro Phe Pro Ala Leu Thr Ala Asp Pro Gly Pro Val Thr
325 330 335
Ser Val Pro Ala Val Pro Pro Ser
340
Claims (4)
1. The manganese peroxidase is used for degrading patulin, wherein the amino acid sequence of the manganese peroxidase is shown as SEQ ID NO. 1.
2. A method for degrading patulin in a foodstuff, the method comprising the step of degrading patulin with a manganese peroxidase having an amino acid sequence as shown in SEQ ID No. 1.
3. The method of degrading patulin in a food product according to claim 2, wherein the food product is an apple product.
4. A method of degrading patulin in a foodstuff according to claim 3, wherein the foodstuff is apple juice or apple jam.
Priority Applications (1)
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