CN113238064A - Application of full-automatic biochemical analyzer in chemical poisoning serum sample detection - Google Patents
Application of full-automatic biochemical analyzer in chemical poisoning serum sample detection Download PDFInfo
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- 210000002966 serum Anatomy 0.000 title claims abstract description 90
- 238000001514 detection method Methods 0.000 title claims abstract description 58
- 206010008428 Chemical poisoning Diseases 0.000 title abstract description 8
- 239000000126 substance Substances 0.000 claims abstract description 60
- 239000002121 nanofiber Substances 0.000 claims abstract description 42
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 30
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 30
- 239000011574 phosphorus Substances 0.000 claims abstract description 30
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 claims abstract description 26
- 239000000460 chlorine Substances 0.000 claims abstract description 26
- 229910052801 chlorine Inorganic materials 0.000 claims abstract description 26
- 238000000034 method Methods 0.000 claims abstract description 26
- FIKAKWIAUPDISJ-UHFFFAOYSA-L paraquat dichloride Chemical compound [Cl-].[Cl-].C1=C[N+](C)=CC=C1C1=CC=[N+](C)C=C1 FIKAKWIAUPDISJ-UHFFFAOYSA-L 0.000 claims abstract description 23
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 claims abstract description 20
- 229920002301 cellulose acetate Polymers 0.000 claims abstract description 18
- 239000002904 solvent Substances 0.000 claims abstract description 15
- 239000000463 material Substances 0.000 claims abstract description 11
- 238000000746 purification Methods 0.000 claims abstract description 10
- 239000002994 raw material Substances 0.000 claims abstract description 8
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims abstract description 3
- 239000008204 material by function Substances 0.000 claims abstract description 3
- 238000003756 stirring Methods 0.000 claims description 38
- 238000001523 electrospinning Methods 0.000 claims description 33
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims description 18
- FJKROLUGYXJWQN-UHFFFAOYSA-N 4-hydroxybenzoic acid Chemical compound OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 claims description 16
- 238000010438 heat treatment Methods 0.000 claims description 16
- 238000009987 spinning Methods 0.000 claims description 11
- 229940090248 4-hydroxybenzoic acid Drugs 0.000 claims description 8
- 238000002360 preparation method Methods 0.000 claims description 8
- JYGLAHSAISAEAL-UHFFFAOYSA-N Diphenadione Chemical compound O=C1C2=CC=CC=C2C(=O)C1C(=O)C(C=1C=CC=CC=1)C1=CC=CC=C1 JYGLAHSAISAEAL-UHFFFAOYSA-N 0.000 claims description 6
- 229960000267 diphenadione Drugs 0.000 claims description 6
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 229910017604 nitric acid Inorganic materials 0.000 claims description 5
- 238000012360 testing method Methods 0.000 claims description 4
- 125000000218 acetic acid group Chemical group C(C)(=O)* 0.000 claims 1
- TURJLPVZRPWSJO-UHFFFAOYSA-N sodium;2-(2,2-diphenylacetyl)inden-2-ide-1,3-dione Chemical compound [Na+].O=C1C2=CC=CC=C2C(=O)[C-]1C(=O)C(C=1C=CC=CC=1)C1=CC=CC=C1 TURJLPVZRPWSJO-UHFFFAOYSA-N 0.000 abstract description 18
- 239000000575 pesticide Substances 0.000 abstract description 7
- 230000035945 sensitivity Effects 0.000 abstract description 5
- 239000012535 impurity Substances 0.000 abstract description 3
- 238000001179 sorption measurement Methods 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 13
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 12
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 9
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical group OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- 235000013305 food Nutrition 0.000 description 9
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 8
- 239000003440 toxic substance Substances 0.000 description 8
- 231100000614 poison Toxicity 0.000 description 6
- 239000003814 drug Substances 0.000 description 5
- 231100000572 poisoning Toxicity 0.000 description 5
- 230000000607 poisoning effect Effects 0.000 description 5
- 230000005180 public health Effects 0.000 description 5
- 238000002798 spectrophotometry method Methods 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 229910002651 NO3 Inorganic materials 0.000 description 3
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 239000003480 eluent Substances 0.000 description 3
- 238000010828 elution Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000007689 inspection Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 125000002777 acetyl group Chemical group [H]C([H])([H])C(*)=O 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 239000009438 liyan Substances 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 231100000167 toxic agent Toxicity 0.000 description 2
- 235000017945 Matricaria Nutrition 0.000 description 1
- 244000042664 Matricaria chamomilla Species 0.000 description 1
- 235000007232 Matricaria chamomilla Nutrition 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- OEBRKCOSUFCWJD-UHFFFAOYSA-N dichlorvos Chemical compound COP(=O)(OC)OC=C(Cl)Cl OEBRKCOSUFCWJD-UHFFFAOYSA-N 0.000 description 1
- 229950001327 dichlorvos Drugs 0.000 description 1
- 238000010041 electrostatic spinning Methods 0.000 description 1
- 239000004009 herbicide Substances 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F1/00—General methods for the manufacture of artificial filaments or the like
- D01F1/02—Addition of substances to the spinning solution or to the melt
- D01F1/10—Other agents for modifying properties
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F2/00—Monocomponent artificial filaments or the like of cellulose or cellulose derivatives; Manufacture thereof
- D01F2/24—Monocomponent artificial filaments or the like of cellulose or cellulose derivatives; Manufacture thereof from cellulose derivatives
- D01F2/28—Monocomponent artificial filaments or the like of cellulose or cellulose derivatives; Manufacture thereof from cellulose derivatives from organic cellulose esters or ethers, e.g. cellulose acetate
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
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- General Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
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Abstract
The invention belongs to the technical field of substance detection, and discloses application of a full-automatic biochemical analyzer in chemical poisoning serum sample detection. In the application process, a full-automatic biochemical analyzer and nano fibers are used; the raw material components for preparing the nano fiber comprise: cellulose acetate, functional materials and solvents; the functional material contains phenolic hydroxyl and carboxyl. According to the invention, the serum sample to be detected is divided into 5 parts, 2 parts of the serum sample are subjected to adsorption and purification treatment by using the nano fibers, so that the interference of other impurities in the serum sample on the detection of organic phosphorus substances and organic chlorine substances in pesticides can be eliminated or greatly reduced, the detection sensitivity of the organic phosphorus substances and the organic chlorine substances is greatly improved, the 5 parts of the serum sample to be detected can be simultaneously detected by using a full-automatic biochemical analyzer, the content of 5 substances, namely paraquat, nitrite, diphacinone sodium salt, organic phosphorus substances and organic chlorine substances in the serum sample to be detected can be known at one time, the time required by detection is greatly shortened, and the detection efficiency is high.
Description
Technical Field
The invention belongs to the technical field of substance detection, and particularly relates to an application of a full-automatic biochemical analyzer in chemical poisoning serum sample detection.
Background
In the process of using toxic substances such as pesticides, herbicides, preservatives and the like, poisoning easily occurs due to irregular operation. In the prior art, only one toxic substance can be detected (only specific toxic substances are detected, and a targeted treatment measure can be taken) in the process of detecting specific toxic substances of a poisoning person in related technologies, the detection of specific toxic substances is too long, and the precious time for saving the poisoning person is easily delayed.
Therefore, it is necessary to provide a method for simultaneously detecting a plurality of toxic substances at one time, which can greatly shorten the time required for detection, and quickly identify the toxic substances corresponding to the poisoned person, thereby greatly improving the detection efficiency.
Disclosure of Invention
The present invention is directed to solving at least one of the problems of the prior art described above. Therefore, the invention provides the application of the full-automatic biochemical analyzer in the detection of the chemically poisoned serum sample, wherein the full-automatic biochemical analyzer and the nano-fiber are simultaneously utilized to detect the serum sample to be detected, and paraquat, nitrite, diphacinone sodium salt, organic phosphorus substances and organic chlorine substances in the serum sample can be simultaneously detected. Namely, the serum sample to be detected is processed by simultaneously using the full-automatic biochemical analyzer and the nano fibers, and the content of 5 substances, namely paraquat, nitrite, diphacinone sodium salt, organic phosphorus substances and organic chlorine substances in the serum sample can be known simultaneously, so that the toxic substance type in the serum sample to be detected can be rapidly determined, the time required by detection is shortened, and the detection efficiency is greatly improved.
The invention conception of the invention is as follows: the invention divides the serum sample to be detected into 5 parts, 2 parts of the serum sample are adsorbed and purified by the nano-fiber, so that the interference of other impurities in the serum sample on the detection of organic phosphorus and organic chlorine substances in pesticides can be eliminated or greatly reduced, the detection sensitivity of the organic phosphorus and the organic chlorine substances is greatly improved, and the organic phosphorus and the organic chlorine substances can be simultaneously detected after the serum sample to be detected is treated by the nano-fiber, so that the detection time is greatly shortened, and the detection efficiency is improved. And then, a full-automatic biochemical analyzer (for example, a full-automatic biochemical analyzer of Shenzhen Meyer biomedical electronics Limited, model number BS-600) is used for simultaneously detecting 5 serum samples to be detected, the contents of 5 substances, namely paraquat, nitrite, diphacinone sodium salt, organic phosphorus substances and organic chlorine substances in the serum samples to be detected can be known at one time, the time required by detection is greatly shortened, and the detection efficiency is high.
The invention provides an application of a full-automatic biochemical analyzer in chemical poisoning serum sample detection.
Specifically, the application of the full-automatic biochemical analyzer in the detection of chemically poisoned serum samples, wherein the full-automatic biochemical analyzer and the nanofibers are used in the application process; the raw material components for preparing the nano-fiber comprise: cellulose acetate, functional materials and solvents; the functional material contains phenolic hydroxyl and carboxyl.
Preferably, the full-automatic biochemical analyzer is a full-automatic biochemical analyzer of Shenzhen Meyer biomedical electronics GmbH, and the model is BS-600.
Preferably, the acetyl content of the cellulose acetate is 38-41%; more preferably, the cellulose acetate has an acetyl group content of 38 to 40%.
Preferably, the functional material is p-hydroxybenzoic acid.
Preferably, the solvent is an organic solvent.
Further preferably, the organic solvent comprises acetone and/or N, N-dimethylformamide.
Preferably, in the raw material components, the mass ratio of the cellulose acetate to the functional material is 1-6: 1; further preferably, the mass ratio of the cellulose acetate to the functional material is 2-4: 1; more preferably, the mass ratio of cellulose acetate to functional material is 3: 1.
Preferably, the diameter of the nanofibers is less than 100 nm; the length is greater than 5mm, for example 6-30 mm.
Preferably, the preparation method of the nanofiber comprises the following steps:
and mixing the cellulose acetate, the functional material and a solvent, heating and stirring to prepare an electrospinning solution, and then spinning to prepare the nanofiber.
Preferably, the temperature of the heating and stirring is 90-105 ℃; further preferably, the temperature of the heating and stirring is 95 to 100 ℃. At this temperature, the electrospinning solution can be uniformly dispersed.
Preferably, the stirring speed of the heating and stirring is 900-1200 rpm; further preferably, the stirring speed of the heating and stirring is 1000-1100 rpm. The proper stirring speed is beneficial to further improving the uniformity of the electrospinning liquid, and has important influence on the finally prepared nano-fiber with high detection sensitivity.
Preferably, the heating and stirring time is 0.5-2 hours; further preferably, the heating and stirring time is 1 to 1.5 hours.
Preferably, the spinning process utilizes an electrospinning device; in the spinning process of the electrospinning device, the working parameters of the electrospinning device are as follows: the needle point voltage is 15-25kV, the receiving polar plate voltage is-2 to-0.5 kV, and the receiving distance is 15-25 cm; further preferably, the operating parameters of the electrospinning device are as follows: the needle point voltage is 18-22kV, the receiving polar plate voltage is-1.5 to-0.5 kV, and the receiving distance is 18-22 cm; more preferably, the operating parameters of the electrospinning device are: the needle point voltage is 20kV, the receiving polar plate voltage is-1 kV, and the receiving distance is 20 cm. The proper working parameters of the electrospinning device are beneficial to preparing the stable nanofiber with good performance, and the improvement of the detection sensitivity of the nanofiber is facilitated.
Preferably, in the process of spinning by using electrospinning equipment, the sampling rate of the electrospinning liquid is 0.2-0.8 mL/h; further preferably, the sample injection rate of the electrospinning liquid is 0.4-0.5 mL/h.
The spinning process by utilizing the electrospinning device belongs to the conventional technology except the working parameters of the electrospinning device.
Preferably, the process of applying comprises the following steps:
dividing a serum sample to be detected into 5 parts, purifying 2 parts of the serum sample by using the nano fibers to obtain 2 parts of the processed serum sample, and then placing the serum sample to be detected in a full-automatic biochemical analyzer for detection.
Preferably, the purification treatment process is as follows: the nanofiber is attached to a stirring rod, then the stirring rod is used for stirring and purifying a serum sample to be detected, organic phosphorus substances or organic chlorine substances in the serum sample to be detected are adsorbed, and then an eluent is used for eluting to obtain 2 parts of treated serum samples (the 2 parts of treated serum samples contain the organic phosphorus substances and the organic chlorine substances).
Further preferably, the purification treatment process comprises: stirring a to-be-detected serum sample for 1 hour by using a stirring rod with a nanofiber attached on the surface, removing the stirring rod, and putting the stirring rod into an eluent (the eluent is methanol containing 0.3% of ethyl acetate by mass fraction) for elution to obtain a purified serum sample (the adsorption rate of the nanofiber prepared by the invention on organic phosphorus substances is close to 100%).
5 parts of serum samples to be detected are detected by different treatment methods and a full-automatic biochemical analyzer with the model number of BS-600, so that whether 5 types of substances, such as paraquat, nitrite, diphacinone sodium salt, organic phosphorus substances and organic chlorine substances, and the content of the substances are contained in the 5 parts of serum samples to be detected can be detected simultaneously.
Preferably, a nitric acid solution or acetonitrile is added to the serum sample to be tested. The addition of the nitric acid solution is favorable for detecting paraquat, and the addition of the acetonitrile is favorable for detecting diphacinone sodium salt and organic phosphorus substances.
A to-be-detected serum sample for detecting paraquat can be used for detecting paraquat J in blood by an ultraviolet spectrophotometry according to the documents 'Baiyun, Fanchangpeng, plum swallow and the like', and the international test medicine journal 2013, 34(11): 1421-1422. "detection of Paraquat by the method described.
Preferably, before the detection is carried out by using a full-automatic biochemical analyzer, according to the calculation of each milliliter of the serum sample to be detected, 250 muL of nitric acid solution with the mass concentration of 12-25% is added, the mixture is kept stand for 3-10 minutes, then the supernatant is taken and adjusted to be alkaline, then the paraquat [ J ] in the blood is determined by an ultraviolet spectrophotometry according to the documents 'Baiyun, Fancheng, plum swallow and the like', and the international journal of inspection medicine 2013, 34(11): 1421-. "detection of Paraquat by the method described.
The serum sample to be detected for detecting nitrite can be used for detecting nitrite according to the method recorded in GB/T5009.33-2008 'determination of nitrite and nitrate in food'.
The serum sample to be detected for detecting the diphacinone sodium salt can be used for quickly detecting the diphacinone in the polluted food according to the literature 'Wangzhongyong, Shixian, J', China public health, 2004, 20(007): 793-. "detection of diphacinone sodium salt by the method described.
Preferably, before the detection is carried out by using a full-automatic biochemical analyzer, 2-6mL of acetonitrile is added according to the calculation of each milliliter of the serum sample to be detected, and then the rapid detection of the diphacinone in the polluted food is carried out according to the literature ' Wangzhongyong, Ganxian and Komaguenza ' J ', Chinese public health, 2004, 20(007): 793-. "detection of diphacinone sodium salt by the method described.
A serum sample to be detected for detecting organic phosphorus substances can be researched according to a method for rapidly detecting organic phosphorus pesticides in poisoning events such as "Liu Jing, Shao Hua and xu Guang" in the literature [ J ], occupation and health, 2010, 26(008): 845-. "detection of organic phosphorus substance by the method described.
Preferably, before the serum sample to be detected for detecting the organic phosphorus substances is detected by using a full-automatic biochemical analyzer, 2-6mL of acetonitrile is added according to the calculation of each milliliter of the serum sample to be detected, and then according to the literature of "Liu Jing, Shao Hua, xu Guang and the like," research on the rapid detection method of the organic phosphorus pesticide in the poisoning event [ J ], occupation and health, 2010, 26(008): 845-. "detection of organic phosphorus substance by the method described.
The serum sample to be detected for detecting organic chlorine substances can be used for detecting residual organic chlorine pesticides [ J ] in food according to the literature, "Lianghongjun, Toonan national defense medicine, 1995(2): 15-17. "detection of organic chlorine substance by the method described in the specification.
The application of the kit is used for detecting the serum sample to be detected containing paraquat, nitrite, diphacinone sodium salt, organic phosphorus substances and/or organic chlorine substances.
Compared with the prior art, the invention has the following beneficial effects:
(1) the invention divides the serum sample to be detected into 5 parts, 2 parts of the serum sample are adsorbed and purified by the nano-fiber, so that the interference of other impurities in the serum sample on the detection of organic phosphorus and organic chlorine substances in pesticides can be eliminated or greatly reduced, the detection sensitivity of the organic phosphorus and the organic chlorine substances is greatly improved, and the organic phosphorus and the organic chlorine substances can be simultaneously detected after the serum sample to be detected is treated by the nano-fiber, so that the detection time is greatly shortened, and the detection efficiency is improved. And then, a full-automatic biochemical analyzer (for example, a full-automatic biochemical analyzer of Shenzhen Meyer biomedical electronics Limited, model number BS-600) is used for simultaneously detecting 5 serum samples to be detected, the contents of 5 substances, namely paraquat, nitrite, diphacinone sodium salt, organic phosphorus substances and organic chlorine substances in the serum samples to be detected can be known at one time, the time required by detection is greatly shortened, and the detection efficiency is high.
(2) The lowest concentration of paraquat which can be detected by the application of the invention is 0.55 mu g/mL, the lowest concentration of nitrite is 1.24 mu g/mL, the lowest concentration of diphacinone sodium salt is 0.029 mu g/mL, the lowest concentration of organophosphorus substance is 3.66 mu g/mL, and the lowest concentration of organic chlorine substance is 1.15 mu g/mL.
Detailed Description
In order to make the technical solutions of the present invention more apparent to those skilled in the art, the following examples are given for illustration. It should be noted that the following examples are not intended to limit the scope of the claimed invention.
The starting materials, reagents or apparatuses used in the following examples are conventionally commercially available or can be obtained by conventionally known methods, unless otherwise specified.
The following electrospinning apparatus was a commercially available conventional electrospinning apparatus (the electrospinning apparatus used was available from Beijing Fumitomo Matricaria technologies, Inc., model FM-1107 electrostatic spinning machine).
Example 1: preparation of nanofibers
A nanofiber is prepared from the following raw material components: 0.3g of cellulose acetate, 0.1g of p-hydroxybenzoic acid and 3mL of a solvent (the solvent is composed of acetone and N, N-dimethylformamide in a volume ratio of 2: 1).
The preparation method of the nanofiber comprises the following steps:
mixing cellulose acetate, p-hydroxybenzoic acid and a solvent, heating and stirring, wherein the heating and stirring temperature is 100 ℃, the heating and stirring speed is 1000 rpm, and the heating and stirring time is 1 hour, preparing an electrospinning solution, and then spinning by using electrospinning equipment, wherein the working parameters of the electrospinning equipment are as follows: the needle point voltage is 20kV, the voltage of a receiving polar plate is-1 kV, the receiving distance is 20cm, and the sampling rate of the electrospinning solution is 0.5mL/h, so as to prepare the nanofiber.
Example 2: preparation of nanofibers
A nanofiber is prepared from the following raw material components: 0.25g of cellulose acetate, 0.15g of p-hydroxybenzoic acid and 3mL of a solvent (the solvent is composed of acetone and N, N-dimethylformamide in a volume ratio of 1: 1).
The preparation method of the nanofiber comprises the following steps:
mixing cellulose acetate, p-hydroxybenzoic acid and a solvent, heating and stirring at the temperature of 105 ℃, at the stirring speed of 1000 rpm for 1.5 hours to prepare an electrospinning solution, and then spinning by using electrospinning equipment, wherein the working parameters of the electrospinning equipment are as follows: the needle point voltage is 22kV, the voltage of a receiving polar plate is-1.5 kV, the receiving distance is 18cm, and the sample injection rate of the electrospinning solution is 0.4mL/h, so as to prepare the nanofiber.
Example 3: preparation of nanofibers
A nanofiber is prepared from the following raw material components: 0.41g of cellulose acetate, 0.11g of p-hydroxybenzoic acid and 4mL of a solvent (the solvent is composed of acetone and N, N-dimethylformamide in a volume ratio of 2: 1).
The preparation method of the nanofiber comprises the following steps:
mixing cellulose acetate, p-hydroxybenzoic acid and a solvent, heating and stirring at the temperature of 95 ℃, the stirring speed of 1100 r/min and the time of 1 hour to prepare an electrospinning solution, and then spinning by using electrospinning equipment, wherein the working parameters of the electrospinning equipment are as follows: the needle point voltage is 20kV, the voltage of a receiving polar plate is-1 kV, the receiving distance is 22cm, and the sampling rate of the electrospinning solution is 0.5mL/h, so as to prepare the nanofiber.
Example 4: application of full-automatic biochemical analyzer in chemical poisoning serum sample detection
The application of the full-automatic biochemical analyzer in the detection of chemically poisoned serum samples comprises the following steps:
taking a serum sample to be detected, dividing the serum sample into 5 parts, and purifying 2 parts of the serum sample by using the nano-fibers prepared in the embodiment 1, wherein the purification process comprises the following steps: stirring a to-be-detected serum sample for 1 hour by using a stirring rod with nanofibers attached to the surface, removing the stirring rod, putting the to-be-detected serum sample into an eluant (the eluant is methanol containing 0.3% of ethyl acetate by mass fraction) for elution to obtain 2 purified serum samples, then carrying out reagent treatment on 5 parts of the to-be-detected serum samples (3 parts of the to-be-detected serum samples are not subjected to the purification treatment, and the to-be-detected serum samples are respectively subjected to ultraviolet spectrophotometry according to the references 'Baiyun, Vangpeng, Liyan and the like', and the paraquat J 'in blood is determined by using an ultraviolet spectrophotometry method, and the international journal of inspection medicine 2013, 34(11): 1421-42', 'GB/T5009.33-2008', the determination of nitrite and nitrate in food ',' Wanghonghongyong, Ganxian, the rapid detection of the diphacinus in the polluted food [ J ], the public health of China, 2004, 20(007): 793-) The analyzer (the model is BS-600) can simultaneously detect (the process of specifically operating the BS-600 full-automatic biochemical analyzer belongs to the conventional operation), and can simultaneously detect the content of 5 types of substances such as paraquat, nitrite, diphacinone sodium salt, organic phosphorus substances and organic chlorine substances.
Example 5: application of full-automatic biochemical analyzer in chemical poisoning serum sample detection
The application of the full-automatic biochemical analyzer in the detection of chemically poisoned serum samples comprises the following steps:
taking a serum sample to be detected, dividing the serum sample into 5 parts, and purifying 2 parts of the serum sample by using the nano-fibers prepared in the embodiment 2, wherein the purification process comprises the following steps: stirring a to-be-detected serum sample for 1 hour by using a stirring rod with nanofibers attached to the surface, removing the stirring rod, putting the to-be-detected serum sample into an eluant (the eluant is methanol containing 0.3% by mass of ethyl acetate) for elution to obtain 2 purified serum samples, then adding 200 muL of nitric acid solution with the mass concentration of 20% into 5 (3 of the serum samples which are not subjected to the purification treatment and 1 of the serum samples which are not subjected to the purification treatment according to the calculation of each milliliter of the to-be-detected serum samples), standing for 5 minutes, taking supernate, adjusting the pH to 8, then treating the to-be-detected serum sample according to the method recorded in the literatures of ' Baiyun, Van Chungpeng, Liyan and the like, and measuring paraquat in blood by using an ultraviolet spectrophotometry [ J ], international inspection medicine journal 2013, 34(11) ' 1-type 1422 ', and in addition, 2 parts of the serum sample refer to the document of ' GB/T5009.33-2008 ' determination of nitrite and nitrate in food The reagent is treated by the method described in' Wangzhongyong, Shixian, and the rapid detection of the enemy mice in the polluted food [ J ], Chinese public health, 2004, 20(007): 793-; 2 parts of serum sample subjected to the purification treatment) is placed in a full-automatic biochemical analyzer (the model is BS-600) for simultaneous detection, and the content of 5 substances such as paraquat, nitrite, diphacinone sodium salt, organic phosphorus substances and organic chlorine substances can be detected simultaneously.
Example 6: application of full-automatic biochemical analyzer in chemical poisoning serum sample detection
Compared with the embodiment 4, the difference of the embodiment 6 is only that 1 part of the serum sample to be detected for detecting the sodium salt of the diphacinone is added with 5mL of acetonitrile according to the calculation of each milliliter of the serum sample to be detected before the detection is carried out by using a full-automatic biochemical analyzer, and then the diphacinone is rapidly detected in the literature' Wangzhongyong, Shixian, contaminated food [ J ], China public health, 2004, 20(007): 793-. "detection of diphacinone sodium salt by the method described.
Product effectiveness testing
Examples 4-6 above were conducted to simultaneously detect the levels of paraquat, nitrite, diphacinone, organophosphorous substance and organochlorine substance class 5, and the lowest concentration of paraquat that could be detected was 0.55. mu.g/mL, the lowest concentration of nitrite was 1.24. mu.g/mL, the lowest concentration of diphacinone sodium salt was 0.029. mu.g/mL, the lowest concentration of organophosphorous substance (dichlorvos) was 3.68. mu.g/mL, and the lowest concentration of organochlorine substance was 1.16. mu.g/mL. Moreover, the method described in examples 4 to 6 takes 120 minutes to simultaneously detect 5 types of paraquat, nitrite, diphacinone sodium salt, organic phosphorus and organic chlorine, and the prior art needs more than 5 hours to detect 5 types of paraquat, nitrite, diphacinone sodium salt, organic phosphorus and organic chlorine in sequence. Therefore, the detection efficiency of the invention is higher.
Claims (10)
1. The application of the full-automatic biochemical analyzer in the detection of chemically poisoned serum samples is characterized in that the full-automatic biochemical analyzer and the nanofibers are used in the application process; the raw material components for preparing the nano-fiber comprise: cellulose acetate, functional materials and solvents; the functional material contains phenolic hydroxyl and carboxyl.
2. Use according to claim 1, wherein the cellulose acetate has an acetyl content of 38-41%; the functional material is p-hydroxybenzoic acid.
3. The use according to claim 1, wherein the mass ratio of cellulose acetate to functional material in the raw material component is 1-6: 1.
4. Use according to claim 1, characterized in that the preparation of said nanofibres comprises the following steps:
and mixing the cellulose acetate, the functional material and a solvent, heating and stirring to prepare an electrospinning solution, and then spinning to prepare the nanofiber.
5. The use according to claim 4, wherein the temperature of the heating and stirring is 90-105 ℃; the stirring speed of the heating and stirring is 900-1200 r/min.
6. The use according to claim 4, wherein the spinning process utilizes an electrospinning apparatus; in the spinning process of the electrospinning device, the working parameters of the electrospinning device are as follows: the needle point voltage is 15-25kV, the receiving polar plate voltage is-2 to-0.5 kV, and the receiving distance is 15-25 cm.
7. The use according to any of claims 1-4, characterized in that it comprises the following steps:
dividing a serum sample to be detected into 5 parts, purifying 2 parts of the serum sample by using the nano fibers to obtain 2 parts of the processed serum sample, and then placing the serum sample to be detected in a full-automatic biochemical analyzer for detection.
8. The use according to claim 7, wherein the purification treatment is carried out by: and attaching the nano fibers to a stirring rod, stirring and purifying the to-be-detected serum sample by using the stirring rod, adsorbing organic phosphorus substances and/or organic chlorine substances in the to-be-detected serum sample, and eluting to obtain 2 parts of the treated serum sample.
9. The use according to claim 7, wherein a nitric acid solution or acetonitrile is added to the test serum sample.
10. The use according to any one of claims 1 to 6 for the detection of a test serum sample containing paraquat, nitrite, diphacinone, organophosphorous substance and/or organochlorine substance.
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Application publication date: 20210810 |