CN1131421C - Miniature biochemical analyzer using dual-spectrum detection - Google Patents
Miniature biochemical analyzer using dual-spectrum detection Download PDFInfo
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- CN1131421C CN1131421C CN 01136250 CN01136250A CN1131421C CN 1131421 C CN1131421 C CN 1131421C CN 01136250 CN01136250 CN 01136250 CN 01136250 A CN01136250 A CN 01136250A CN 1131421 C CN1131421 C CN 1131421C
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Abstract
The present invention relates to a miniature biochemical analyzer using dual-spectrum detection, which belongs to the technical field of photoelectric light spectrum detection and light spectrum biochemical analysis and comprises a self-adaptive adjustable light source, an optical attenuator, a first sample chamber, a first integrated optical micro spectrometer and a first analogue-to-digital converter which are orderly connected to the same light path by optical elements; the analyzer also comprises a second sample chamber, a second integrated optical micro spectrometer, a second analogue-to-digital converter and a computer data processing system, wherein the second sample chamber, the second integrated optical micro spectrometer and the second analogue-to-digital converter are connected to the other light path by optical elements and the optical attenuator, and the computer data processing system is respectively connected with the two analogue-to-digital converters, the optical attenuator and the self-adaptive adjustable light source. The analyzer can enlarge the dynamic range of photoelectric light spectrum detection and realize the functions of self calibration, real-time error correction, etc. of a self-adaptive dynamic regulation system. The miniature biochemical analyzer system using dual-spectrum detection can be used for conventional biochemical detection and field biochemical analysis; furthermore, the analyzer has the potential application on the fields of biological medical treatment, environmental monitoring, material analysis, etc.
Description
Technical field the invention belongs to photoelectric spectrum and surveys and spectrum biochemical analysis technical field; Be particularly related to the structural design of spectral detection Biochemical Analyzer.
Common biochemical instruments of background technology and the similar instrument of spectrophotometer all have only a cover monochromator system, and the system that has has two cover monochromators, but detector to be two-way shared and do not possess the performance of linear array detector.
A kind of two spectrometer systems as color sensor (USP5,793,486) are the instruments of measuring as paper color.System has adopted two spectrometers.A high-reflection region (white area) of aiming at paper, another aims at the high absorbing area (black area) of paper.Two spectrometers are measured the full spectrum in two zones respectively simultaneously.This system is suitable for the color and the characteristic relevant with color of on-line measurement campaign paper.System carries out contrast ratio reflection measurement, and the real-time correction of the full spectrum of opaque article is provided.Because the structure of this system is limit and can not be adopted common light channel structure, simultaneously-measured is the spectrum of sample zones of different.Lack the function that calibration system baseline and noise are eliminated automatically.Therefore this system is not suitable for carrying out the measurement and the biochemical analysis of biological sample.
Summary of the invention the objective of the invention is to overcome the weak point of prior art, a kind of miniature biochemical analyzer using dual-spectrum detection is proposed, functions such as dynamic range, the realization self-adaptation that can enlarge the photoelectric spectrum detection dynamically adjusted, system's demarcation certainly, Real-time Error correction.
A kind of miniature biochemical analyzer using dual-spectrum detection that the present invention proposes, it is characterized in that, comprise by optical element being connected to self-adaptation tunable light source, optical attenuator, first sample chamber, the first integrated optics low-light spectrometer and first analog to digital converter in the same light path in turn, also comprise by optical element and this optical attenuator being connected second sample chamber, the second integrated optics low-light spectrometer and second analog to digital converter in another light path; Be connected in the computer data processing system of these two analog to digital converters, said optical attenuator and self-adaptation tunable light source respectively; Said self-adaptation tunable light source makes it produce the brightness that adapts with sample in order to regulate supply voltage by feedback signal; Said optical attenuator is realized the self-adaptation of light beam is regulated by transmitance and attenuation change; Said first, second integrated optics low-light spectrometer forms the outgoing beam of sample and standard model in first and second sample chamber the light distribution of continuous spectrum respectively, as measuring-signal and reference signal, and deliver to said first and second analog to digital converter and convert corresponding digital signal to; This reference signal is used to carry out real-time FEEDBACK CONTROL, system from demarcation, error correction; Said computer data processing system will be handled through two kinds of digital signals after the analog to digital converter conversion, realize detection in real time, Measurement and Data Processing, biochemical indicator analysis, intelligent data diagnosis and evaluation, document management.
Light source in the self-adaptation tunable light source of the present invention can adopt the wide range lamps such as Halogen lamp LED of launching the wide range light beam; Controllable power can change output power by regulating output voltage, and the power supply supply that makes light source obtain different voltages and power produces different brightness.Heat sink can adopt fan, is installed near the light source, and the heat that light source is sent by forced draft in time distributes, and reduces environment temperature.Hygrosensor, spectral detector, light intensity detector and light source controller all can adopt conventional products, and wherein, hygrosensor is installed near the light source, becomes electric signal to give controller the temperature transition that detects.Light intensity detector is installed in the light source back side, gives controller with the light intensity signal that detects.Spectral detector detects particular spectral lines, gives controller with detectable signal.Behind the measuring-signal that hygrosensor, light intensity detector and the spectral detector that light source controller receives sent here, reduce or the voltage of rising power supply and blower, increase and reduce its power input, reach the purpose of control ambient humidity, light and temperature by the circuit feedback.For example when detector measurement when environment temperature is too high in system, controller improves the fan power input and increases exhaust air rate, reduces light source power and reduces heating, and environment temperature is reduced.When detector measurement was too high to brightness, controller can reduce light source power power, reduces brightness.When spectral detector measures the saturated signal to noise ratio (S/N ratio) of spectral line when too low, controller can reduce and improve light source power, or the prompting user that gives the alarm more renews spare parts such as bulb.Also can set the controlled variable of light source according to the requirement of measuring samples, as given voltage, power etc.By the gamut dynamic range optimize, light intensity/spectrum corresponding point range is optimized, other optimize criterion, makes measuring system be in optimum condition all the time.Avoided to obtain peak-data, also avoided simultaneously having flooded useful signal because of signal to noise ratio (S/N ratio) is too poor because of saturated.This self-adaptation FEEDBACK CONTROL has improved measurement sensitivity and enlarged measures dynamic range.
Continuous or the stepped change transmissivity optical element of said optical attenuator can be that rectangle glass adopts known technology to plate the metallic diaphragm of uneven gradual change, and its transmittance also is gradual change.Light beam shines diverse location and has different light transmission rates.By mechanical adjustment mechanism, as adopt known miniature transfer table to move continuously or stepped change transmissivity optical element, then can realize the transmitance and the attenuation change of light beam.Continuously same or stepped change transmissivity optical element also can be made circular discoid continuous gradation transmitance and distribute.To be contained in the rotating shaft of a mechanical adjustment mechanism with rotating shaft, light beam is mapped on the optical attenuation dish, and when the optical attenuation disc spins, transmitance changes, and has realized the adjusting to light beam.If the present invention is the optical attenuator that is made of components and parts such as other photoelectricity, acousto-optic, optical polarizations.By changing the polarizer and analyzer relative angle etc., light beam is changed by the attenuation coefficient of optical attenuator.Its governor motion can be subjected to the FEEDBACK CONTROL of detection signal by controller, by machinery move, rotation, acousto-optic modulator frequency adjustment, optical polarization device relative angle change and adjust light transmission rate, realize light intensity is carried out special parameter or real-time adjusting.
The integrated optics low-light spectrometer that the present invention adopts is the photoelectric spectrum basic components, and light beam is directive diffraction colour astigmatism grid after optical fiber is coupled into spectrometer, and the light distribution that forms continuous spectrum through the chromatic dispersion grating scattering arrives array photelectric receiver surface.The corresponding different spectral line composition in each unit of receiver detects corresponding line strength signal.Usually adopt 256 cellular array detectors.Spectral resolution is better than 7nm.Dynamic range is better than 104.A low-light spectrometer is used for the measuring samples spectral distribution, and another low-light spectrometer is used to measure comparative sample.Comparative sample can be vacuum, air, standard model and other are with reference to sample etc.Reference signal is used to carry out real-time FEEDBACK CONTROL, system from demarcation, error correction etc.Also can measure a sample simultaneously with two low-light spectrometers.Obtain the multiline data and carry out data processing, realize reducing functions such as noise.
Said data handling system can be a mini-computer and detection, analysis, the evaluation calculation machine software systems that are stored in the custom-designed biochemical indicator of employing routine techniques wherein.And possess necessary parameter and sample data storehouse.Can detect in real time, Measurement and Data Processing, biochemical indicator analysis, intelligent data diagnosis and evaluation, document management etc.
Principle of work of the present invention is as follows:
The light that wide spectrum light source sends is coupled into optical system.Light beam passes sample by absorption of sample, scattering and reflection.Light beam through sample box is coupled into spectrometer through optical fiber.Light beam arrives the detector array surface through the scattering of micro spectrometer dispersion element, and the detector array receiving spectrum distributes, and spectral signal is transformed into electric signal.
Light beam passes optical attenuator before entering the sample chamber.This optical attenuator is made up of continuous or stepped change transmissivity optical element and mechanical adjustment mechanism.Also can form by components and parts such as other photoelectricity, acousto-optic, polarizations.Optical attenuator makes the light beam that enters the sample chamber be subjected to certain decay.Governor motion is subjected to the FEEDBACK CONTROL of detection signal, carries out special parameter or real-time adjusting to seeing through light intensity.One side sample difference, its transmitance difference; The strength difference of each spectral line is very big on the other hand, and the dynamic range of detector is limited.For the detection that adapts to various light intensity with all spectral lines are characterized clear, adjust optical attenuator according to the variation of absorption spectrum and the intensity of spectral line, make to arrive detector intensity and make it always work in best dynamic.
Measuring method of the present invention is identical with spectrophotometer dual-wavelength measurement method based on same principle.
Common single, double light beam spectrophotometer can not be eliminated the influence of non-characteristic absorption signal, thereby causes measuring error.The dual-wavelength measurement method has improved sensitivity and accuracy when measuring the quantitative test of high concentration sample and muddy sample and polycomponent biased sample.
The dual-wavelength spectrophotomer principle is as follows: the light from same light source is divided into two bundles, respectively through two monochromators, obtains two bundles and has different wave length (λ
1, λ
2) monochromatic light, utilize chopper to make the same sample cell that shines that two-beam replaces at a certain time interval.The absorbance difference Δ A=A of the two-beam that measures
12One A
11As long as λ
1, λ
2Select suitably, promptly a wavelength is the absorbing material maximum, and another wavelength is for absorbing seldom or not absorbing, and Δ A is an absorbance (having deducted " background absorption ") of having eliminated non-characteristic absorption influence.
Be located in the dual-wavelength measurement, the two-beam that incides sample cell is identical by force, and corresponding wavelength is respectively λ
1And λ
2, the absorbance school timetable of two kinds of samples is shown:
A
11＝ε
11Cb+A
S1
A
12＝ε
12Cb+A
S2
A in the formula
SBe background absorption.ε
11And ε
12Be molar absorption coefficient, when solution concentration was unit with volumetric molar concentration (mol/L), printing opacity thickness was unit with cm, and absorption law is expressed as: A=ε Cb.ε represents that solution concentration is 1mol/L, the absorbance of this material during photic zone thickness 1cm, and this material extinction of the big more expression of ε ability is strong more.C is a solution concentration, and b is the printing opacity liquid layer thickness.
If λ
1And λ
2, select suitably, then can think A
S1=A
S2=A
S, promptly background absorption is identical, and the absorbance log difference of the two-beam in transmission sample pond is:
ΔA＝A
12-A
11＝(ε
12-ε
11)Cb
Obvious Δ A ∝ C.The foundation of dual-wavelength spectrophotometry quantitative test that Here it is.
Miniature biochemical instruments of the present invention is made up of two structure integrated optics low-light spectrometers identical with performance.A low-light spectrometer is used for the spectral distribution of measuring samples, and spectral signal is changed into electric signal, sends into computing machine and handles.Another low-light spectrometer is used to measure comparative sample.The comparison sample can be vacuum, air, standard model, reference sample etc.Measured reference signal enters computing machine through opto-electronic conversion and handles.The reference signal data are as the foundation of carrying out optical attenuator FEEDBACK CONTROL, system's demarcation certainly, Real-time Error correction etc.Also can measure a sample simultaneously with two low-light spectrometers.Obtain the multiline data and carry out data processing, realize reducing functions such as noise.
The present invention has following characteristics:
1. the self-adaptation tunable light source can be to temperature when measuring, and the requirement of brightness etc. is carried out feedback adjusting to power.Its role is to improve sensitivity and enlarge and measure dynamic range.
2. optical attenuator is subjected to the FEEDBACK CONTROL of detection signal, carries out special parameter or real-time adjusting to seeing through light intensity.Its role is to adjust incident intensity, is that measuring system is in optimum condition.
3. two cover performances identical integrated optics low-light spectrometers are formed this miniature biochemical instruments system.One is used for measuring samples, and another is used for carrying out real-time FEEDBACK CONTROL, demarcation, error correction etc. certainly as the reference signal.
Data handling system can detect in real time, Measurement and Data Processing, biochemical indicator analysis, intelligent data diagnosis and evaluation, document management etc.And possess whole measuring system, comprise light source, optical attenuator, low-light spectrometer parameter setting etc., carry out the function of real-time adaptive control.
5. owing to adopted two integrated optics low-light spectrometer systems, system calibrating, Real-time Error are proofreaied and correct and are realized full-automation, need not manual intervention.Instrument is stable more more reliable.
6. owing to adopted integrated optics low-light spectrometer technology, the whole instrument system bulk is little, compact conformation, be easy to carry.
The present invention can be used for routine biochemistry detection, on-the-spot biochemical analysis.In many fields such as biologic medical, environmental monitoring, material analysiss potential application is arranged all.
Description of drawings:
Fig. 1 is miniature biochemical analyzer using dual-spectrum detection embodiment 1 structural representation of the present invention.
Fig. 2 is miniature biochemical analyzer using dual-spectrum detection embodiment 2 structural representations of the present invention.
Fig. 3 is a self-adaptation tunable light source embodiment synoptic diagram of the present invention.
Fig. 4 is an optical attenuator embodiment synoptic diagram of the present invention.
Fig. 5 is an integrated optics micro spectrometer example structure synoptic diagram of the present invention.
Fig. 6 is the data processing software flow chart of present embodiment.
Embodiment
The miniature biochemical analyzer using dual-spectrum detection that the present invention proposes reaches accompanying drawing in conjunction with the embodiments and is described in detail as follows:
The structure of embodiment 1 as shown in Figure 1.The miniature biochemical instruments of present embodiment is made up of two structure integrated optics low-light spectrometers identical with performance.One is used for measuring samples, another witness mark comparison sample.Also can measure a sample simultaneously with two low-light spectrometers.Can carry out real-time FEEDBACK CONTROL, demarcation certainly, error correction, reduction noise function etc. by the processing of reference signal.
Self-adaptation tunable light source 1 is launched the wide range light beam among Fig. 1, and line focus collimation lens 2 and optical attenuator 3 arrive spectroscope 4.Transmitted light beam enters sample chamber 5, by absorption of sample, reflection and scattering.Enter optical fiber 7 from the light beam of sample chamber outgoing through convergent lens 6.Optical fiber 7 is introduced the first integrated optics micro spectrometer 8 with light beam.Light beam is received and converts to electric signal by array photodetectors 9 after the dispersion element scattering.Electric signal converts digital signal to through analog to digital converter 10 and sends into computing machine 18.After catoptron 11 reflections, enter comparison sample chamber 12 through spectroscope 4 beam reflected, emergent light is coupled into low-light spectrometer 15 after condenser 13 enters optical fiber 14, convert electric signal to through array photodetectors 16 and send into analog to digital converter 17, send into computing machine 18 again.The measurement data that 18 pairs of two spectrometers of computing machine are sent here is handled, analyzes, is estimated.Data such as simultaneous computer is also saturated to light intensity, signal, signal to noise ratio (S/N ratio) are analyzed, and send feedback control signal 19 and 20 according to measurement requirement to self-adaptation tunable light source 1 and optical attenuator 2.
A kind of modified embodiment 2 of the present invention, as shown in Figure 2.Present embodiment comprises two low-light spectrometers independently, and its structure mainly is to add spectroscope 101 and spectroscope 102 in the basic light path of embodiment 1.Low- light spectrometer 8 and 15 can be respectively measured simultaneously sample 5 and reference sample 12 and is compared, and also can carry out the continuous spectrum scanning survey simultaneously by low- light spectrometer 8 and 15 pairs of same samples 5.Owing to adopt array optoelectronic scanning device, spectral scan and photosignal conversion are carried out with very high frequency.For example: more than the 1KHz.Make the instable influence of environment and sample very little, noise is very low.
Low-light spectrometer among the present invention is continuous spectrum scanning, according to any component characteristics absorption data of measured sample, adopts any amount and any specific wavelength numerical value to carry out data processing, realizes the data processing more complicated more than dual wavelength.As described in the principle part, if λ
1And λ
2, select suitably, then can think A
S1=A
S2=A, promptly background absorption is identical, through aforesaid simple computation, then can eliminate ground unrest, improves sensitivity.
The self-adaptation tunable light source embodiment that the above-mentioned two kinds of embodiment of the present invention adopt is made up of light source 35, controllable power 37, fan 32, hygrosensor 36, light intensity detector 33, spectral detector 34, light source controller 38 as shown in Figure 3.Wherein, light source 35 can be wide range lamps such as Halogen lamp LED, launches the wide range light beam as light source of the present invention.Controllable power 37 can change output power by regulating output voltage, and the power supply supply that makes light source 35 obtain different voltages and power produces different brightness.Fan 32 is installed near the light source 35, and the heat that light source 35 is sent by forced draft in time distributes, and reduces environment temperature.Hygrosensor 36 is installed near the light source 35, becomes electric signal to give controller 38 temperature transition that detects.Light intensity detector 33 is installed in the light source back side, gives controller 38 with the light intensity signal that detects.34 pairs of particular spectral lines of spectral detector detect, and give controller 38 with detectable signal.Controller 38 is connected with computing machine 39.Behind the measuring-signal that hygrosensor 36, light intensity detector 33 and the spectral detector 34 that light source controller 38 receives sent here, signal is sent into computing machine 39, send the voltage of corresponding instruction by computing machine by light source controller 38 reductions or rising power supply 35 and fan 32, increase and reduce its power input, reach the purpose of control ambient humidity, light and temperature.For example when detector measurement when environment temperature is too high in system, controller 38 improves fans 32 power inputs and increases exhaust air rate, reduces light source power and reduces heating, and environment temperature is reduced.When detector 33 measures brightness when too high, controller 38 can reduce light sources 35 powers, reduces brightness.When spectral detector 34 measures the saturated signal to noise ratio (S/N ratio) of spectral line when too low, controller 38 can reduce and improve light source power, or the prompting user that gives the alarm more renews spare parts such as bulb.Also can set the controlled variable of light source according to the requirement of measuring samples, as given voltage, power etc.By the gamut dynamic range optimize, light intensity/spectrum corresponding point range is optimized, other optimize criterion, makes measuring system be in optimum condition all the time.Avoided to obtain peak-data, also avoided simultaneously having flooded useful signal because of signal to noise ratio (S/N ratio) is too poor because of saturated.This self-adaptation FEEDBACK CONTROL has improved measurement sensitivity and enlarged measures dynamic range.
The embodiment of three kinds of optical attenuators of the present invention as shown in Figure 4.
Embodiment 3 is shown in Fig. 4 c, and wherein, optical attenuator is made of the polarizer 48 that places coaxial position and analyzer 49, and when the polarizer 48 centered on axis 50 relative rotations with analyzer 49 by drive unit, transmitted light can change.
The position of the optical attenuator 42 of above-mentioned three kinds of embodiment, the rotation angle of disk attenuator 45 and the polarizer 48 and all corresponding transmissivity parameter of analyzer 49 relative rotation angle also are subjected to the control of adaptive system.Displacement drive is connected with computing machine with rotating driving device, sends control signal by computing machine and realizes FEEDBACK CONTROL.
The example structure of integrated optics micro spectrometer of the present invention and principle are as shown in Figure 5.Its structure is made of integrated optics chromatic dispersion grating 53 and array photodetectors 54.Whole micro spectrometer be adopt integrated optics fabrication techniques in a slice integrated electro chip, compact conformation, volume are little, reliability improves.Its principle of work is: incident beam 51 is coupled into optical fiber 52, and optical fiber imports light beam into the low-light spectrometer.The light beam 55 that enters spectrometer is mapped on the integrated optics chromatic dispersion grating 53.Through the dispersion interaction and the reflection of grating, the spectrum of light beam 56 is presented on the array photodetectors 54.Array photodetectors 54 converts spectral distribution to electric signal 57 and delivers to computing machine.The optical fiber coupling improves stability of layout.
Above-mentioned low-light spectrometer can be chosen the low-light spectrometer chip product of German MicroParts company.The technical indicator of low-light spectrometer can be as follows: spectral range: 300-800nm or be increased to 280-1900nm; Blaze wavelength: 560nm; Level is inferior: the first order; Resolution: 7nm; Physical dimension: 14mm * 23mm; Coupled fiber: 50-125 μ m; Numerical aperture: 0.22; Fiber lengths: 1-1.5m; Detector array: 256 points; Field-scanning period 1KHz.
The computing machine that the present invention adopts can be general microcomputer, and wherein Cun Chu data processing software program as shown in Figure 6.This data processor is detection, interpretation of result, the data evaluation computer software that adopts the custom-designed biochemical indicator of routine techniques means.And possess necessary parameter and sample data storehouse.Software is made up of 3 parts: measure and data processing; The reagent sample storehouse; Light source and optical attenuator control module.Measure with data processing module and be provided with by system's initial parameter; Measurement data; The characteristic spectrum data analysis; Biochemical indicator is analyzed; The interpretation of result medical diagnosis on disease; The result shows; Set up data file; Data output; Compositions such as data network transmission.The reagent sample library module is by the reagent database; The sample data storehouse; Correction coefficient; Compositions such as computing formula.Light source and optical attenuator control module are by the light source detection data; The light source control data; Compositions such as optical attenuator control.
System's initial parameter is provided with and can comprises: light source setting, measurement range, measuring period, sample temperature, instrument reference data, user profile data etc.Measurement data is absorption of sample data, sample temperature data, ambient temperature data of apparatus measures etc.The characteristic spectrum data analysis module is analyzed measurement data.Call standard reagent database, sample data storehouse, correction coefficient, computing formula during analysis.These all adopt biochemical analysis normal data and method.Carry out the biochemical indicator analysis according to the absorptiometry data.Measurement result is analyzed and possible disease diagnosed.Measurement and analysis result can show and save as document output and Network Transmission.Database can upgrade, and does statistical study, sets up statistical sample and template etc.
These software systems can be sent the light source control data according to system parameter setting and light source detection data simultaneously, for example: improve and reduce bulb voltage, improve and reduce rotation speed of the fan etc.Also can send the control data about attenuator, optical attenuator is moved or turn to ad-hoc location, transmitted light is by needed certain proportion decay.
The present invention can finish the test of various biochemical indicators; Optical fiber is coupled into the low-light spectrometer can realize the microscopic spectrum analysis; Two spectrometers can be realized dynamically adjusting from demarcation, light intensity, the automatic treatment for correcting of detection equal error and self-adaptation; The field scan speed of 256 point measurement 1KHz obtains continuous spectrum information can realize light intensity one spectral analysis; The intelligent data system carries out the data analysis and the processing of spectrum and biochemical indicator.
Miniature biochemical analyzer using dual-spectrum detection of the present invention system can be used for that routine biochemistry detects, on-the-spot biochemical analysis.All has potential using value in many fields such as biologic medical, environmental monitoring, nutrition, medicine and pharmacology, material analysis, semiconductor, judicial expertises.
Claims (7)
1. miniature biochemical analyzer using dual-spectrum detection, it is characterized in that, comprise by optical element being connected to self-adaptation tunable light source, optical attenuator, first sample chamber, the first integrated optics low-light spectrometer and first analog to digital converter in the same light path in turn, also comprise by optical element and this optical attenuator being connected second sample chamber, the second integrated optics low-light spectrometer and second analog to digital converter in another light path; Be connected in the computer data processing system of these two analog to digital converters, said optical attenuator and self-adaptation tunable light source respectively; Said self-adaptation tunable light source makes it produce the brightness that adapts with sample in order to regulate supply voltage by feedback signal; Said optical attenuator is realized the self-adaptation of light beam is regulated by transmitance and attenuation change; Said first, second integrated optics low-light spectrometer forms the outgoing beam of sample and standard model in first and second sample chamber the light distribution of continuous spectrum respectively, as measuring-signal and reference signal, and deliver to said first and second analog to digital converter and convert corresponding digital signal to; This reference signal is used to carry out real-time FEEDBACK CONTROL, system from demarcation, error correction; Said computer data processing system will be handled through two kinds of digital signals after the analog to digital converter conversion, realize detection in real time, Measurement and Data Processing, biochemical indicator analysis, intelligent data diagnosis and evaluation, document management.
2. miniature biochemical analyzer using dual-spectrum detection as claimed in claim 1, it is characterized in that, said self-adaptation tunable light source comprises light source, the control power supply of adjustable output voltage links to each other with this light source, near heat sink, hygrosensor, spectral detector, the light intensity detector of this light source setting, and the light source controller that links to each other with said heat sink, hygrosensor, spectral detector, light intensity detector; Said light source is the wide range lamp.
3. miniature Biochemical Analyzer as claimed in claim 1 is characterized in that, said optical attenuator comprises continuously or the mechanical adjustment mechanism of stepped change transmissivity optical element and coupled examined signal feedback control forms.
4. miniature Biochemical Analyzer as claimed in claim 1 is characterized in that, said optical attenuator comprises that the mechanical adjustment mechanism that photoelectricity, acousto-optic or optical polarization element and coupled examined signal feedback are controlled forms.
5. miniature Biochemical Analyzer as claimed in claim 1 is characterized in that said computer data processing system comprises that a microcomputer and preliminary election are stored in real-time detection, Measurement and Data Processing, biochemical indicator analysis, intelligent data diagnosis and evaluation, document management wherein; Software module.
6. miniature Biochemical Analyzer as claimed in claim 1, it is characterized in that, said optical element comprises the focussed collimated lens that are arranged between this light source and the optical attenuator, be separately positioned on first, second spectroscope of this optical attenuator and first, second sample chamber one side, be separately positioned on two convergent lenses of this first, second sample chamber opposite side, and be arranged on the optical fiber that two ends between two convergent lenses and two micro spectrometers have coupling mechanism.
7. the integrated optics low-light spectrometer of stating as claim 6 is characterized in that, said optical element also comprises the 3rd, the 4th spectroscope that is separately positioned between these two sample chambers and two convergent lenses.
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| CN 01136250 CN1131421C (en) | 2001-10-12 | 2001-10-12 | Miniature biochemical analyzer using dual-spectrum detection |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2013185282A1 (en) * | 2012-06-11 | 2013-12-19 | 保生国际生医股份有限公司 | Biochemical detecting system and light source module thereof |
| CN102901707A (en) * | 2012-07-26 | 2013-01-30 | 苏州天和自动化系统有限公司 | Spectrum detection rod capable of continuously measuring |
| CN102914449A (en) * | 2012-09-17 | 2013-02-06 | 广州市怡文环境科技股份有限公司 | Double-light path colorimetric real-time sampling device |
| CN103499391B (en) * | 2013-09-06 | 2016-08-10 | 清华大学 | Spectral measurement system |
| CN103499393B (en) * | 2013-09-06 | 2015-10-21 | 清华大学 | The measuring method of spectrum |
| CN103776787A (en) * | 2014-02-25 | 2014-05-07 | 杭州纳宏光电科技有限公司 | Double-spectrum water quality analyzer |
| CN103968943B (en) * | 2014-04-24 | 2016-02-03 | 中国电子科技集团公司第四十一研究所 | A kind of accurate measurement method of fiber spectrometer signal to noise ratio (S/N ratio) |
| US9739661B2 (en) * | 2015-06-30 | 2017-08-22 | Agilent Technologies, Inc. | Infrared imaging system with automatic referencing |
| WO2018134723A1 (en) | 2017-01-19 | 2018-07-26 | Agilent Technologies, Inc. | Optical spectrometer modules, systems and methods for optical analysis with multiple light beams |
| CN108955881A (en) * | 2018-06-28 | 2018-12-07 | 佛山市方垣机仪设备有限公司 | A kind of novel spectrometer |
| CN109405971A (en) * | 2018-10-18 | 2019-03-01 | 南京邮电大学 | A kind of micro- polarization spectrum analysis system and method |
| CN110296974A (en) * | 2019-07-16 | 2019-10-01 | 钇斯特激光科技(天津)有限责任公司 | Quantitative LIBS laser system and quantitative approach |
| CN110530516A (en) * | 2019-09-12 | 2019-12-03 | 山东东仪光电仪器有限公司 | A kind of light splitting dispersion means for inductive coupling plasma emission spectrograph |
| CN113736654A (en) * | 2021-09-03 | 2021-12-03 | 重庆市盛佰昱科技有限公司 | Light source assembly and observation system for time difference incubator |
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