CN112986602B - Full-automatic blood analysis and component separation system - Google Patents
Full-automatic blood analysis and component separation system Download PDFInfo
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- CN112986602B CN112986602B CN202110166565.7A CN202110166565A CN112986602B CN 112986602 B CN112986602 B CN 112986602B CN 202110166565 A CN202110166565 A CN 202110166565A CN 112986602 B CN112986602 B CN 112986602B
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- 238000000926 separation method Methods 0.000 title claims abstract description 70
- 238000004159 blood analysis Methods 0.000 title claims abstract description 18
- 210000004369 blood Anatomy 0.000 claims abstract description 282
- 239000008280 blood Substances 0.000 claims abstract description 282
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 47
- 230000001954 sterilising effect Effects 0.000 claims abstract description 29
- 238000005070 sampling Methods 0.000 claims abstract description 17
- 238000007689 inspection Methods 0.000 claims abstract description 16
- 238000000034 method Methods 0.000 claims abstract description 16
- 210000002381 plasma Anatomy 0.000 claims description 53
- 238000007710 freezing Methods 0.000 claims description 29
- 238000005119 centrifugation Methods 0.000 claims description 28
- 210000003743 erythrocyte Anatomy 0.000 claims description 25
- 210000001772 blood platelet Anatomy 0.000 claims description 23
- 239000007788 liquid Substances 0.000 claims description 19
- 238000012423 maintenance Methods 0.000 claims description 13
- 230000010355 oscillation Effects 0.000 claims description 13
- 238000004321 preservation Methods 0.000 claims description 13
- 238000001514 detection method Methods 0.000 claims description 12
- 210000004623 platelet-rich plasma Anatomy 0.000 claims description 12
- 238000012360 testing method Methods 0.000 claims description 9
- 239000000725 suspension Substances 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 4
- 230000000249 desinfective effect Effects 0.000 claims description 3
- 238000010241 blood sampling Methods 0.000 abstract description 3
- 239000000306 component Substances 0.000 description 60
- 230000008014 freezing Effects 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 230000001276 controlling effect Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 230000000740 bleeding effect Effects 0.000 description 2
- 239000012503 blood component Substances 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 229940127219 anticoagulant drug Drugs 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000001174 ascending effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000004023 fresh frozen plasma Substances 0.000 description 1
- 238000005213 imbibition Methods 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000003761 preservation solution Substances 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/00722—Communications; Identification
- G01N35/00732—Identification of carriers, materials or components in automatic analysers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/08—Radiation
- A61L2/10—Ultraviolet radiation
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
- B01D17/02—Separation of non-miscible liquids
- B01D17/0217—Separation of non-miscible liquids by centrifugal force
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5002—Partitioning blood components
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1095—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers
- G01N35/1097—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers characterised by the valves
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2202/00—Aspects relating to methods or apparatus for disinfecting or sterilising materials or objects
- A61L2202/20—Targets to be treated
- A61L2202/22—Blood or products thereof
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N2001/002—Devices for supplying or distributing samples to an analysing apparatus
- G01N2001/005—Packages for mailing or similar transport of samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/00722—Communications; Identification
- G01N35/00732—Identification of carriers, materials or components in automatic analysers
- G01N2035/00821—Identification of carriers, materials or components in automatic analysers nature of coded information
- G01N2035/00831—Identification of carriers, materials or components in automatic analysers nature of coded information identification of the sample, e.g. patient identity, place of sampling
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
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Abstract
The invention relates to the technical field of whole blood treatment, and particularly discloses a full-automatic blood analysis and component separation system, which comprises a blood bag temperature control sterilizing box, a blood sample temperature control sterilizing box, a sterilizing conveying channel, a sampling and checking mechanism and a blood bag component separation mechanism, wherein the blood bag temperature control sterilizing box and the blood sample temperature control sterilizing box respectively store whole blood bags and whole blood small samples obtained by all blood sampling stations, sterilize and keep cold for batch transportation; the disinfection conveying channel conveys the blood bag temperature control disinfection box and the blood sample temperature control disinfection box to specific positions, so that the sampling and inspection mechanism can acquire the whole blood sample for inspection, and the blood bag component separation mechanism can acquire the whole blood bag passing the whole blood sample inspection for component separation. The whole checking and separating process does not need human participation, the labor cost is low, the checking and separating efficiency is high, and the separating precision is also high.
Description
Technical Field
The invention relates to the technical field of whole blood treatment, in particular to a full-automatic blood analysis and component separation system.
Background
The main process of separating the collected whole blood into various component blood comprises the following steps:
1) Extracting 200-400 ml and trace amount of whole blood from a donor, respectively storing the whole blood in empty bags and empty vials added with anticoagulant and other maintenance components, and uniformly storing the whole blood in a special storage box;
2) At the blood station, firstly, a small amount of blood in a small tube is inspected, and the whole blood bag corresponding to the qualified blood sample is subjected to multi-step component separation.
The separation method has the advantages of dispersed steps, higher human participation, high labor cost, low separation efficiency, timeliness of certain blood components, preparation within 6-8 hours after whole blood collection, short preparation time after the intermediate transportation time is removed, and higher pressure on related staff.
Disclosure of Invention
The invention provides a full-automatic blood analysis and component separation system, which solves the technical problems that: how to automatically realize the blood analysis and component separation of whole blood.
In order to solve the above technical problems, the present invention provides a full-automatic blood analysis and component separation system, comprising: a blood bag temperature control sterilizing box, a blood sample temperature control sterilizing box, a sterilizing conveying channel, a sampling and checking mechanism and a blood bag component separating mechanism;
the blood bag temperature control disinfection box is used for storing collected whole blood bags, and is used for storing whole blood small samples which are in one-to-one correspondence with the whole blood bags stored in the blood bag temperature control disinfection box; the disinfection conveying channel is used for disinfecting the blood bag temperature control disinfection box and the blood sample temperature control disinfection box and conveying the blood bag temperature control disinfection box and the blood sample temperature control disinfection box to a preset position; the sampling and checking mechanism is used for extracting blood in the whole blood sample and checking to confirm whether the corresponding whole blood bag is available or not; the blood bag component separation mechanism is used for separating components of the available whole blood bags.
Specifically, the whole blood bag comprises a bag body, a bag opening and a switch valve arranged at the bag opening, wherein the bag opening is made of hard materials, and the bag opening is opened or closed by operating the switch valve;
the blood bag temperature control disinfection box is uniformly provided with a plurality of blood bag compartments matched with the whole blood bag in shape, and one blood bag compartment corresponds to one whole blood bag;
the blood sample temperature control disinfection box is uniformly provided with a plurality of blood sample compartments matched with the shape of the whole blood sample, and one blood sample compartment corresponds to one whole blood sample.
Specifically, the sampling inspection mechanism comprises a first upward clamping structure, a first label reading structure, a puncture liquid suction structure and an analyzer;
the first upward clamping structure is used for clamping whole blood small samples in the blood sample temperature control disinfection box in batches;
the first label reading structure is used for reading the identification labels of a plurality of whole blood platelets in the process of upwards clamping by the first upwards clamping structure and synchronizing the identification labels to the analyzer and a background server connected with the analyzer;
the puncture liquid suction structure is used for entering a plurality of whole blood samples in a needle tube puncture mode when the first upward clamping structure reaches a fixed station, and sucking corresponding blood into the analyzer;
the analyzer is used for testing the blood in a plurality of whole blood platelets and marking the results and reasons of passing and failing tests in the background server.
Specifically, the blood bag component separation structure comprises a second upward clamping structure, a second label reading structure, a flow dividing structure, a centrifugal structure, an upper and lower clamping structure, a switch valve control structure, a component separation structure and a preservation structure;
the second upward clamping structure is used for batchwise clamping the whole blood bags in the blood bag temperature control disinfection box in a bag clamping opening mode;
the second label reading structure is used for reading the identification labels of the whole blood bags in the process of upwards clamping by the second upwards clamping structure and synchronizing the identification labels to the background server;
the split-flow structure is used for matching the detection result of the analyzer with the whole blood bags clamped by the second upward clamping structure in a background server, conveying the whole blood bags which do not pass the detection and pass the detection separately, and placing the whole blood bags which pass the detection into the centrifugal structure;
the centrifugal structure is used for carrying out heavy centrifugation or light centrifugation treatment on the placed whole blood bags;
the upper and lower clamping structure is used for clamping the whole blood bag in the centrifugal structure after centrifugation to the switch valve adjusting position or putting the whole blood bag in the centrifugal structure again;
the on-off valve control structure is used for controlling the on-off valve of the whole blood bag at the on-off valve adjusting position, and the control content comprises opening or closing of the bag mouth of the corresponding whole blood bag;
the component separation structure is used for separating components from the whole blood bag with the bag opening opened by the switch valve control structure;
the preservation structure is used for preserving the component blood bags separated by the component separation structure.
Specifically, the centrifugal structure comprises a first centrifuge, and the component separation structure comprises a first separator;
the first centrifugal machine is used for carrying out first re-centrifugal treatment on the placed whole blood bags;
the first separator is used for sucking the red blood cells in the whole blood bag after the first re-centrifugation is opened into a blank blood bag added with maintenance liquid to obtain a first suspended red blood cell blood bag; at this time, the whole blood bag is sealed by the on-off valve control structure as the first blood plasma bag.
Specifically, when macroscopic red blood cells are still mixed in the first plasma blood bag, the component separation structure further comprises a second separator;
the upper and lower clamping structure is also used for placing the first plasma blood bag into the first centrifugal machine;
the first centrifuge is also used for carrying out secondary heavy centrifugation treatment on the first blood plasma bag;
and the second separator is used for sucking the blood plasma in the process into the blank blood bag added with the maintenance liquid after the first blood plasma blood bag after the second re-centrifugation is opened, so as to obtain a second blood plasma blood bag.
Specifically, the preservation structure comprises a first quick-freezing box, and the first quick-freezing box is used for quick-freezing and preserving the first blood plasma blood bag and/or the second blood plasma blood bag.
Specifically, the centrifugal structure comprises a second centrifuge, and the component separation structure comprises a third centrifuge;
the second centrifugal machine is used for carrying out light centrifugal treatment on the placed whole blood bags for the first time;
the third separator is used for sucking the red blood cells in the whole blood bag after the first light centrifugation into a blank blood bag added with maintenance liquid after the whole blood bag is opened to obtain a second suspended red blood cell blood bag; at this time, the whole blood bag is sealed by the on-off valve control structure as a platelet-rich plasma blood bag.
Specifically, the component separation structure further comprises a fourth separator;
the upper and lower clamping structure is also used for placing the platelet-rich plasma blood bag into the second centrifugal machine;
the second centrifuge is also used for carrying out first re-centrifugation treatment on the placed platelet-rich plasma blood bag;
the fourth separator is used for sucking the blood plasma in the blood plasma bag containing the maintenance liquid after the platelet-rich blood plasma blood bag after the first re-centrifugation is opened, so as to obtain a third blood plasma blood bag; leaving the platelet rich plasma blood bag room warm for at least 1 hour to serve as a concentrated platelet suspension blood bag.
Specifically, the preservation structure comprises a second quick-freezing box and an oscillation constant temperature device, wherein the second quick-freezing box is used for quick-freezing and preserving the third blood plasma blood bag, and the oscillation constant temperature device is used for carrying out oscillation preservation on the concentrated platelet suspension blood bag in an environment of 22+/-2 degrees.
The invention provides a full-automatic blood analysis and component separation system, which comprises a blood bag temperature control sterilizing box, a blood sample temperature control sterilizing box, a sterilizing transmission channel, a sampling inspection mechanism and a blood bag component separation mechanism, wherein the blood bag temperature control sterilizing box and the blood sample temperature control sterilizing box respectively store whole blood bags and whole blood small samples obtained by all blood sampling stations, sterilize and cool the whole blood bags and the whole blood small samples, and are convenient for batch transportation; the disinfection conveying channel conveys the blood bag temperature control disinfection box and the blood sample temperature control disinfection box to specific positions, so that the sampling and inspection mechanism can acquire the whole blood sample for inspection, and the blood bag component separation mechanism can acquire the whole blood bag passing the whole blood sample inspection for component separation. The whole checking and separating process does not need human participation, the labor cost is low, the checking and separating efficiency is high, and the separating precision is also high.
Drawings
FIG. 1 is a block diagram of a fully automated blood analysis and component separation system provided in an embodiment of the present invention;
FIG. 2 is a block diagram of the sample inspection mechanism of FIG. 1 provided in accordance with an embodiment of the present invention;
FIG. 3 is a block diagram of the blood bag component separation mechanism of FIG. 1 provided in an embodiment of the present invention;
FIG. 4 is a flow chart of a blood bag component separation mechanism for separating components of whole blood bags to obtain suspended red blood cell blood bags and plasma blood bags according to an embodiment of the present invention;
fig. 5 is a flowchart of a blood bag component separation mechanism according to an embodiment of the present invention for separating components from a whole blood bag to obtain a suspended red blood cell blood bag, a concentrated platelet blood bag, and a plasma blood bag.
Detailed Description
The following examples are given for the purpose of illustration only and are not to be construed as limiting the invention, including the drawings for reference and description only, and are not to be construed as limiting the scope of the invention as many variations thereof are possible without departing from the spirit and scope of the invention.
The embodiment of the invention provides a full-automatic blood analysis and component separation system, as shown in a structure diagram in fig. 1, comprising: a blood bag temperature control sterilizing box, a blood sample temperature control sterilizing box, a sterilizing conveying channel, a sampling and checking mechanism and a blood bag component separating mechanism.
The blood bag temperature control disinfection box is used for storing collected whole blood bags, and the blood sample temperature control disinfection box is used for storing whole blood small samples which are in one-to-one correspondence with the whole blood bags stored in the blood bag temperature control disinfection box; the disinfection conveying channel is used for disinfecting (such as ultraviolet disinfection) the blood bag temperature control disinfection box and the blood sample temperature control disinfection box and conveying the blood bag temperature control disinfection box and the blood sample temperature control disinfection box to a preset position; the sampling and checking mechanism is used for extracting blood in the whole blood sample and checking to confirm whether the corresponding whole blood bag is available or not; the blood bag component separation mechanism is used for separating components of the available whole blood bags.
Specifically, the whole blood bag comprises a bag body, a bag opening and a switch valve arranged at the bag opening, wherein the bag opening is made of hard materials, and the bag opening is opened or closed by operating the switch valve. The bag mouth adopts hard materials to facilitate the sampling inspection mechanism and the blood bag component separation mechanism to carry out clamping, and is convenient to move according to a preset program. The design of the switch valve is convenient for controlling the opening and closing of the bag opening when the blood bag component separation mechanism performs component separation, and is convenient for transporting the whole blood bag and centrifuging when the bag opening is closed, and the matched straw stretches into the blood which is layered after centrifuging when the bag opening is opened so as to absorb specific components. And the control of the bag opening and closing can lead the blood bag component separation mechanism to carry out centrifugation and separation for a plurality of times, thereby meeting the requirements of preparing the blood with various components.
Specifically, the blood bag temperature control disinfection box is uniformly provided with a plurality of blood bag compartments matched with the shape of the whole blood bag, and one blood bag compartment corresponds to one whole blood bag. The temperature control disinfection box for the blood bag is designed to be convenient for fixing the position of the whole blood bag, limit the bag mouth of the whole blood bag to be always positioned at the upper fixed position and facilitate the clamping of the follow-up blood bag component separating mechanism.
Specifically, the blood sample temperature control disinfection box of the embodiment is uniformly provided with a plurality of blood sample compartments matched with the shape of the whole blood sample, one blood sample compartment corresponds to one whole blood sample and is also positioned at a position for limiting the whole blood sample, so that a sampling and checking mechanism is convenient to clamp and puncture for taking liquid.
For the disinfection conveying channel, a mode of ultraviolet disinfection is adopted, and conveying belts are adopted for conveying. When the blood sample temperature control sterilizing box and the blood bag temperature control sterilizing box are placed at the set positions of the sterilizing conveying channels, the doors of the blood sample temperature control sterilizing box and the blood bag temperature control sterilizing box are required to be opened.
Specifically, in this embodiment, as shown in fig. 2, the sampling inspection mechanism includes a first upward gripping structure, a first tag reading structure, a puncture pipetting structure and an analyzer;
the first upward clamping structure is used for clamping and taking whole blood platelets in the blood sample temperature control disinfection box in batches;
the first label reading structure is used for reading the identification labels of a plurality of whole blood platelets in the process of upwards clamping by the first upwards clamping structure and synchronizing the identification labels to a background server connected with the analyzer;
the puncture liquid suction structure is used for entering a plurality of whole blood samples in a needle tube puncture mode when the first upward clamping structure reaches the fixed station, and sucking corresponding blood into the analyzer;
the analyzer is used to test blood in a plurality of whole blood platelets and to mark the results and reasons of the test passing and failing in a background server.
Here, the tong of structure is got to upwards pressing from both sides suits with the bottle lid of whole blood sample, and the syringe needle of puncture imbibition structure is comparatively sharp-pointed, can easily impale the bottle lid of whole blood sample, based on the height of bottle, and the syringe needle stays in the bottom 4mm department apart from the whole blood sample. The first tag reading structure is then set based on the tag on the whole blood sample. In order to read the label on the whole blood sample at one hundred percent, after the whole blood sample is clamped by the clamping hand of the first upward clamping structure, the whole blood sample is rotated at a slower speed by the first upward clamping structure in the rising process. It should also be noted that the device that is in direct contact with the blood during the test is disposable and the entire test is in a sterile environment.
The analyzer detects the blood sample, and for a qualified blood sample, sends corresponding information to the component separation structure, which separates components only for a qualified whole blood bag and conveys an unqualified whole blood bag to an unqualified area.
Specifically, in this embodiment, as shown in fig. 3, the blood bag component separation structure includes a second upward gripping structure, a second tag reading structure, a diverting structure, a centrifugal structure, an up-down gripping structure, a switching valve control structure, a component separation structure, and a preserving structure;
the second upward clamping structure is used for batchwise clamping the whole blood bags in the temperature control disinfection box of the blood bags in a mode of clamping the bag openings;
the second label reading structure is used for reading the identification labels of the plurality of whole blood bags in the process of upwards clamping by the second upwards clamping structure and synchronizing the identification labels to the background server;
the split-flow structure is used for matching the detection result of the analyzer with the whole blood bag clamped by the second upward clamping structure in the background server, conveying the whole blood bags which do not pass the detection and pass the detection separately, and placing the whole blood bags which pass the detection into the centrifugal structure;
the centrifugal structure is used for carrying out heavy centrifugation or light centrifugation treatment on the placed whole blood bags;
the upper and lower clamping structure is used for clamping the whole blood bag in the centrifugal structure after centrifugation to the regulating position of the switch valve or putting the whole blood bag in the centrifugal structure again;
the on-off valve control structure is used for controlling the on-off valve of the whole blood bag at the on-off valve adjusting position, and the control content comprises opening or closing of the bag mouth of the corresponding whole blood bag;
the component separation structure is used for separating components from the whole blood bag with the bag opening opened by the switch valve control structure;
the preservation structure is used for preserving the component blood bags separated by the component separation structure.
The second upward clamping structure clamping hand is matched with the mouth of the whole blood bag. The second label reading structure is provided based on the label on the whole blood bag (the label on the whole blood sample and the whole blood bag are identical). Also, in order to read one hundred percent of the label on the whole blood sample, the whole blood bag is rotated at a slower speed in the ascending process after the whole blood bag is gripped by the grip of the second upward gripping structure. It should also be noted that the devices that are in direct contact with the blood during the separation process are disposable and the entire testing process is in a sterile environment.
The suspension red blood cells are also called as red blood cell suspension, are prepared by adding red blood cell preservation solution after most of plasma is removed from whole blood through centrifugation, and are the red blood cell preparation with the largest clinical dosage at present. Fresh frozen plasma (quick frozen plasma) is prepared by separating plasma within 6-8 hours after collection, and quick freezing in an alcohol bath or a quick-frozen refrigerator with an air cooling device at below-30deg.C, or quick freezing with liquid nitrogen at-196 deg.C.
Taking fig. 4 as an example, in order to successfully prepare suspended red blood cells and quick frozen plasma, the centrifugal structure of the present embodiment includes a first centrifuge, and the component separation structure includes a first separator;
the first centrifugal machine is used for carrying out first re-centrifugal treatment on the placed whole blood bag, wherein the upper part is blood plasma (faint yellow), the lower part is red blood cells (dark red), and a very thin layer of white blood cells and platelets (white) are arranged at the joint of the two parts;
the first separator is used for sucking the red blood cells in the whole blood bag after the first re-centrifugation into a blank blood bag added with maintenance liquid after the whole blood bag is opened to obtain a first suspended red blood cell blood bag; at this time, the whole blood bag is sealed with the on-off valve control structure as the first blood plasma bag.
The component separation structure further comprises a second separator when macroscopic red blood cells are still mixed in the first plasma blood bag;
the upper and lower clamping structure is also used for placing the first plasma blood bag into a first centrifugal machine;
the first centrifugal machine is also used for carrying out secondary re-centrifugation treatment on the placed first plasma blood bag;
the second separator is used for sucking the blood plasma in the first blood plasma blood bag after the second re-centrifugation into the blank blood bag added with the maintenance liquid after the first blood plasma blood bag is opened, so as to obtain a second blood plasma blood bag.
The preservation structure comprises a first quick-freezing box, and the first quick-freezing box is used for quick-freezing and preserving the first blood plasma blood bag and/or the second blood plasma blood bag.
It should be noted that the "macroscopic red blood cells" are not necessarily human to observe in the present embodiment, and this can be determined by an image processing technique.
The concentrated platelet is mainly used for stopping bleeding and preventing bleeding clinically, and the existing preparation method generally comprises the following steps: whole blood is collected by using a multi-connected blood collection bag, and then platelets are separated by using a high-capacity centrifuge at 20-24 ℃ within 6-8 hours and suspended in plasma to prepare concentrated platelets. While the present embodiment takes an additional approach.
Taking fig. 5 as an example, in order to successfully produce suspended red blood cells, concentrated platelet suspensions, and frozen plasma, the centrifuge structure includes a second centrifuge and the component separation structure includes a third separator;
the second centrifugal machine is used for carrying out a first light centrifugal treatment on the placed whole blood bags;
the third separator is used for sucking the red blood cells in the whole blood bag after the first light centrifugation into a blank blood bag added with maintenance liquid after the whole blood bag is opened to obtain a second suspended red blood cell blood bag; at this time, the whole blood bag is sealed with the on-off valve control structure as the platelet-rich plasma blood bag.
The component separation structure further comprises a fourth separator;
the upper and lower clamping structure is also used for placing the platelet-rich plasma blood bag into a second centrifugal machine;
the second centrifugal machine is also used for carrying out first re-centrifugal treatment on the placed platelet-rich plasma blood bag;
the fourth separator is used for sucking the blood plasma in the blood plasma bag which is rich in platelets and added with maintenance liquid after the platelet-rich blood plasma bag after the first re-centrifugation is opened, so as to obtain a third blood plasma bag; the remaining platelet rich plasma blood bag is left to stand for at least 1 hour (typically 1-2 hours) as a concentrated platelet suspension blood bag.
The preservation structure comprises a second quick-freezing box and an oscillation constant temperature device, wherein the second quick-freezing box is used for quick-freezing and preserving the third plasma blood bag, and the oscillation constant temperature device is used for carrying out oscillation preservation on the concentrated platelet suspension blood bag in an environment of 22+/-2 degrees.
The first to fourth separators of this embodiment adopt the same structure, and first quick freezing case and second quick freezing case adopt the same structure, before putting into quick freezing case, to the blood bag that does not paste the label, automatic for it paste the same label with original blood bag, convenient tracing to the source. The blood bag of each component obtained after separation is also required to be labeled with a component label, so that the subsequent use is facilitated. It should also be noted that the whole blood component separation process is performed in a single use as long as the device is in direct contact with the blood, and the whole separation process is in a sterile environment.
The first quick-freezing box, the second quick-freezing box and the oscillation constant temperature device are not limited in quantity, and because the blood is separated in batches, the first quick-freezing box, the second quick-freezing box and the oscillation constant temperature device can be designed corresponding to each batch, and each first quick-freezing box, each second quick-freezing box and each oscillation constant temperature device are designed in a layered type, so that the blood bag can be taken and placed conveniently. In particular, for the oscillation constant temperature device, the embodiment designs a vibration device at the bottom of the box body, which can drive the whole box body with the blood bag to vibrate stably.
In summary, the full-automatic blood analysis and component separation system provided by the embodiment of the invention comprises a blood bag temperature control sterilizing box, a blood sample temperature control sterilizing box, a sterilizing conveying channel, a sampling inspection mechanism and a blood bag component separation mechanism, wherein the blood bag temperature control sterilizing box and the blood sample temperature control sterilizing box respectively store whole blood bags and whole blood small samples obtained by all blood sampling stations, sterilize and keep cold, and facilitate batch transportation; the disinfection conveying channel conveys the blood bag temperature control disinfection box and the blood sample temperature control disinfection box to specific positions, so that the sampling and inspection mechanism can acquire the whole blood sample for inspection, and the blood bag component separation mechanism can acquire the whole blood bag passing the whole blood sample inspection for component separation. The whole checking and separating process does not need human participation, the labor cost is low, the checking and separating efficiency is high, and the separating precision is also high.
The above examples are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above examples, and any other changes, modifications, substitutions, combinations, and simplifications that do not depart from the spirit and principle of the present invention should be made in the equivalent manner, and the embodiments are included in the protection scope of the present invention.
Claims (7)
1. A fully automated blood analysis and component separation system comprising: a blood bag temperature control sterilizing box, a blood sample temperature control sterilizing box, a sterilizing conveying channel, a sampling and checking mechanism and a blood bag component separating mechanism;
the blood bag temperature control disinfection box is used for storing collected whole blood bags, and is used for storing whole blood small samples which are in one-to-one correspondence with the whole blood bags stored in the blood bag temperature control disinfection box; the disinfection conveying channel is used for disinfecting the blood bag temperature control disinfection box and the blood sample temperature control disinfection box and conveying the blood bag temperature control disinfection box and the blood sample temperature control disinfection box to a preset position; the sampling and checking mechanism is used for extracting blood in the whole blood sample and checking to confirm whether the corresponding whole blood bag is available or not; the blood bag component separation mechanism is used for separating components of the available whole blood bags;
the whole blood bag comprises a bag body, a bag opening and a switch valve arranged at the bag opening, wherein the bag opening is made of hard materials, and the bag opening is opened or closed by operating the switch valve;
the blood bag temperature control disinfection box is uniformly provided with a plurality of blood bag compartments matched with the whole blood bag in shape, and one blood bag compartment corresponds to one whole blood bag;
the blood sample temperature control disinfection box is uniformly provided with a plurality of blood sample compartments matched with the shape of the whole blood sample, and one blood sample compartment corresponds to one whole blood sample;
the sampling inspection mechanism comprises a first upward clamping structure, a first label reading structure, a puncture liquid suction structure and an analyzer;
the first upward clamping structure is used for clamping whole blood small samples in the blood sample temperature control disinfection box in batches;
the first label reading structure is used for reading the identification labels of a plurality of whole blood platelets in the process of upwards clamping by the first upwards clamping structure and synchronizing the identification labels to the analyzer and a background server connected with the analyzer;
the puncture liquid suction structure is used for entering a plurality of whole blood samples in a needle tube puncture mode when the first upward clamping structure reaches a fixed station, and sucking corresponding blood into the analyzer;
the analyzer is used for testing the blood in a plurality of whole blood platelets and marking the results and reasons of passing and failing tests in the background server;
the blood bag component separation structure comprises a second upward clamping structure, a second label reading structure, a flow dividing structure, a centrifugal structure, an upper and lower clamping structure, a switch valve control structure, a component separation structure and a preservation structure;
the second upward clamping structure is used for batchwise clamping the whole blood bags in the blood bag temperature control disinfection box in a bag clamping opening mode;
the second label reading structure is used for reading the identification labels of the whole blood bags in the process of upwards clamping by the second upwards clamping structure and synchronizing the identification labels to the background server;
the split-flow structure is used for matching the detection result of the analyzer with the whole blood bags clamped by the second upward clamping structure in a background server, conveying the whole blood bags which do not pass the detection and pass the detection separately, and placing the whole blood bags which pass the detection into the centrifugal structure;
the centrifugal structure is used for carrying out heavy centrifugation or light centrifugation treatment on the placed whole blood bags;
the upper and lower clamping structure is used for clamping the whole blood bag in the centrifugal structure after centrifugation to the switch valve adjusting position or putting the whole blood bag in the centrifugal structure again;
the on-off valve control structure is used for controlling the on-off valve of the whole blood bag at the on-off valve adjusting position, and the control content comprises opening or closing of the bag mouth of the corresponding whole blood bag;
the component separation structure is used for separating components from the whole blood bag with the bag opening opened by the switch valve control structure;
the preservation structure is used for preserving the component blood bags separated by the component separation structure.
2. A fully automated blood analysis and component separation system according to claim 1, wherein: the centrifuge structure comprises a first centrifuge, and the component separation structure comprises a first separator;
the first centrifugal machine is used for carrying out first re-centrifugal treatment on the placed whole blood bags;
the first separator is used for sucking the red blood cells in the whole blood bag after the first re-centrifugation is opened into a blank blood bag added with maintenance liquid to obtain a first suspended red blood cell blood bag; at this time, the whole blood bag is sealed by the on-off valve control structure as the first blood plasma bag.
3. The fully automated blood analysis and component separation system of claim 2, wherein the component separation structure further comprises a second separator while still mixing macroscopic red blood cells in the first plasma blood bag;
the upper and lower clamping structure is also used for placing the first plasma blood bag into the first centrifugal machine;
the first centrifuge is also used for carrying out secondary heavy centrifugation treatment on the first blood plasma bag;
and the second separator is used for sucking the blood plasma in the process into the blank blood bag added with the maintenance liquid after the first blood plasma blood bag after the second re-centrifugation is opened, so as to obtain a second blood plasma blood bag.
4. A fully automated blood analysis and component separation system according to claim 3, wherein:
the preservation structure comprises a first quick-freezing box, and the first quick-freezing box is used for quick-freezing and preserving the first blood plasma blood bag and/or the second blood plasma blood bag.
5. A fully automated blood analysis and component separation system according to any one of claims 1 to 4, wherein: the centrifugal structure comprises a second centrifuge, and the component separation structure comprises a third separator;
the second centrifugal machine is used for carrying out light centrifugal treatment on the placed whole blood bags for the first time;
the third separator is used for sucking the red blood cells in the whole blood bag after the first light centrifugation into a blank blood bag added with maintenance liquid after the whole blood bag is opened to obtain a second suspended red blood cell blood bag; at this time, the whole blood bag is sealed by the on-off valve control structure as a platelet-rich plasma blood bag.
6. A fully automated blood analysis and component separation system according to claim 5, wherein: the component separation structure further comprises a fourth separator;
the upper and lower clamping structure is also used for placing the platelet-rich plasma blood bag into the second centrifugal machine;
the second centrifuge is also used for carrying out first re-centrifugation treatment on the placed platelet-rich plasma blood bag;
the fourth separator is used for sucking the blood plasma in the blood plasma bag containing the maintenance liquid after the platelet-rich blood plasma blood bag after the first re-centrifugation is opened, so as to obtain a third blood plasma blood bag; leaving the platelet rich plasma blood bag room warm for at least 1 hour to serve as a concentrated platelet suspension blood bag.
7. A fully automated blood analysis and component separation system according to claim 6, wherein: the preservation structure comprises a second quick-freezing box and an oscillation constant temperature device, wherein the second quick-freezing box is used for quick-freezing and preserving the third blood plasma blood bag, and the oscillation constant temperature device is used for carrying out oscillation and preserving on the concentrated platelet suspension blood bag.
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| CN117679569B (en) * | 2024-02-04 | 2024-05-03 | 北京麦邦天工医疗技术有限公司 | Blood treatment apparatus |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202119662U (en) * | 2011-05-04 | 2012-01-18 | 北京上惠好生物技术有限公司 | Platelet-rich plasma preparing and detecting device |
| CN103354755A (en) * | 2010-12-30 | 2013-10-16 | 美国血液技术公司 | System and method for collecting platelets and anticipating plasma return |
| CN103501853A (en) * | 2011-05-06 | 2014-01-08 | 美国血液技术公司 | System and method for automated separation of whole blood |
| CN203535690U (en) * | 2013-09-30 | 2014-04-09 | 烟台海深威医学技术有限公司 | Full-automatic blood reserved sample checking system |
| CN104155435A (en) * | 2013-04-15 | 2014-11-19 | 贝克顿·迪金森公司 | Biological fluid collection device and biological fluid separation and testing system |
| CN105816928A (en) * | 2016-05-27 | 2016-08-03 | 四川南格尔生物科技有限公司 | Blood composition acquisition device and method |
| CN108414777A (en) * | 2018-02-08 | 2018-08-17 | 前海瑞智捷自动化科技（深圳）有限公司 | A kind of blood sample test assembly line and method go out sample equipment |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2547436T3 (en) * | 2010-03-19 | 2015-10-06 | Asahi Kasei Medical Co., Ltd. | Cell Extraction Procedure |
| JP7495360B2 (en) * | 2019-02-15 | 2024-06-04 | テルモ株式会社 | Bag set |
-
2021
- 2021-02-04 CN CN202110166565.7A patent/CN112986602B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103354755A (en) * | 2010-12-30 | 2013-10-16 | 美国血液技术公司 | System and method for collecting platelets and anticipating plasma return |
| CN202119662U (en) * | 2011-05-04 | 2012-01-18 | 北京上惠好生物技术有限公司 | Platelet-rich plasma preparing and detecting device |
| CN103501853A (en) * | 2011-05-06 | 2014-01-08 | 美国血液技术公司 | System and method for automated separation of whole blood |
| CN104155435A (en) * | 2013-04-15 | 2014-11-19 | 贝克顿·迪金森公司 | Biological fluid collection device and biological fluid separation and testing system |
| CN203535690U (en) * | 2013-09-30 | 2014-04-09 | 烟台海深威医学技术有限公司 | Full-automatic blood reserved sample checking system |
| CN105816928A (en) * | 2016-05-27 | 2016-08-03 | 四川南格尔生物科技有限公司 | Blood composition acquisition device and method |
| CN108414777A (en) * | 2018-02-08 | 2018-08-17 | 前海瑞智捷自动化科技（深圳）有限公司 | A kind of blood sample test assembly line and method go out sample equipment |
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